標題: Titlebook: Contemporary Approaches to the Study of Pain; From Molecules to Ne Rebecca P. Seal Book 2022 Springer Science+Business Media, LLC, part of [打印本頁] 作者: Cleveland 時間: 2025-3-21 17:14
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作者: Semblance 時間: 2025-3-21 23:40 作者: Consequence 時間: 2025-3-22 03:29
Visualizing Synaptic Connectivity Using Confocal and Electron Microscopy : Neuroanatomical Approachpter are to provide detailed descriptions of some of these protocols, to highlight both advantages and potential difficulties that may be encountered when using these approaches, and to reference several studies where these have been applied to study spinal circuits in normal and chronic pain states.作者: Urologist 時間: 2025-3-22 05:45
Optical Imaging of the Spinal Cord for the Study of Pain : From Molecules to Neural Networks,alized in these preparations as can the inputs from primary sensory neurons. We also include a detailed protocol used by our laboratory to monitor cells in laminae I and II outer in mice as well as a discussion of the limitations of cell imaging in the spinal cord.作者: CREEK 時間: 2025-3-22 12:44 作者: aerobic 時間: 2025-3-22 16:51
Nachweis und Bestimmung von Fermenten,nd how they may influence sensory transduction. In this chapter, we describe a powerful electrophysiological technique that allows for ex vivo recordings from intact primary muscle afferents from two target muscle groups, similar to that previously described for cutaneous afferents.作者: aerobic 時間: 2025-3-22 17:34
https://doi.org/10.1007/978-3-663-04972-2e the lumbar spinal cord by laminectomy and then stereotaxically inject the dissociated cells into the dorsal horn. Our studies have demonstrated that 2?weeks posttransplantation, MGE cells are fully differentiated into mature GABAergic interneurons and have functionally integrated into the host spinal circuitry.作者: 阻礙 時間: 2025-3-23 00:49
Wilhelm Sturtzel,Hermann Schmidt-Stiebitzenerate a Type17 immune response and that this response was protective against the spread of injury (Cohen et al. Cell 178(4):919–32 e14, 2019). Here, we describe the mouse models, stimulation protocols and equipment used in these studies.作者: jaundiced 時間: 2025-3-23 03:42 作者: FEAS 時間: 2025-3-23 09:32
Single-Unit Electrophysiological Recordings of Primary Muscle Sensory Neurons Using a Novel Ex Vivond how they may influence sensory transduction. In this chapter, we describe a powerful electrophysiological technique that allows for ex vivo recordings from intact primary muscle afferents from two target muscle groups, similar to that previously described for cutaneous afferents.作者: 連鎖 時間: 2025-3-23 09:46
Intraspinal Transplantation of Precursors of Cortical GABAergic Interneurons to Treat Neuropathic Pe the lumbar spinal cord by laminectomy and then stereotaxically inject the dissociated cells into the dorsal horn. Our studies have demonstrated that 2?weeks posttransplantation, MGE cells are fully differentiated into mature GABAergic interneurons and have functionally integrated into the host spinal circuitry.作者: 價值在貶值 時間: 2025-3-23 16:09
,Use of Optogenetics for the Study of Skin–Nerve Communication,enerate a Type17 immune response and that this response was protective against the spread of injury (Cohen et al. Cell 178(4):919–32 e14, 2019). Here, we describe the mouse models, stimulation protocols and equipment used in these studies.作者: DIS 時間: 2025-3-23 20:19
Book 2022our laboratory..Cutting-edge and comprehensive,?.Contemporary Approaches to the Study of Pain: From Molecules to Neural Networks?.is a valuable resource for scientists and researchers interested in making impactful contributions to our understanding of pain..作者: 贊成你 時間: 2025-3-24 02:16
Book 2022y cutting-edge methods including the identification of gene expression profiles, transcriptomes or translatomes, from individual cells or defined groups of cells in rodents and primates; ?the electrophysiological investigation of human tissues, such as human dorsal root ganglion neurons; ways to ass作者: 雀斑 時間: 2025-3-24 04:17
0893-2336 ation advice from the experts.This volume contains experimental approaches that are currently revolutionizing our understanding of the neurobiology of pain. The chapters cover many cutting-edge methods including the identification of gene expression profiles, transcriptomes or translatomes, from ind作者: GROWL 時間: 2025-3-24 06:55
,Die Verzinnungsf?higkeit von Feinblechen,cribe a robust, relatively fast and low cost, turnkey in situ hybridization technology (ISH) to identify one or more RNA targets together with immunohistochemical analyses. Specifically, we present a customized version of the protocol that works particularly well for spinal cord and primary sensory ganglia tissues.作者: 神圣將軍 時間: 2025-3-24 14:29 作者: detach 時間: 2025-3-24 15:45
Contemporary Approaches to the Study of Pain978-1-0716-2039-7Series ISSN 0893-2336 Series E-ISSN 1940-6045 作者: 明智的人 時間: 2025-3-24 21:42
https://doi.org/10.1007/978-3-663-04975-3ffer significantly to the environments in intact animals. Also, traditional methods like patch clamp electrophysiology are very low throughput. In vivo calcium imaging of peripheral ganglia strives to overcome these limitations. In this chapter, we will discuss the development and procedures associated with this powerful new technique.作者: 辮子帶來幫助 時間: 2025-3-24 23:54 作者: Demonstrate 時間: 2025-3-25 03:25
978-1-0716-2041-0Springer Science+Business Media, LLC, part of Springer Nature 2022作者: Negligible 時間: 2025-3-25 10:33 作者: 短程旅游 時間: 2025-3-25 15:07 作者: Feedback 時間: 2025-3-25 19:42 作者: Offstage 時間: 2025-3-25 22:29
Gemeinschaftsausschu? Verzinken, Düsseldorfd with tagged ribosomes in specific cell types, in vivo. We have used Nav1.8-Cre mice crossed with mice that have a floxed L10a-GFP fusion. Because L10a protein is associated with translating ribosomes, this crossing generates mice with tagged ribosomes exclusively expressed in Nav1.8-positive neuro作者: cathartic 時間: 2025-3-26 00:34
Nachweis und Bestimmung von Fermenten,both normal and pathological conditions. With these strategies, experimenters are able to quantify afferent response properties, such as threshold and firing rates, and determine the functional classifications of individual cells. New research techniques have been developed that allow for both chara作者: 生來 時間: 2025-3-26 04:52
https://doi.org/10.1007/978-3-663-04504-5istently high rate at which therapeutic targets fail to translate into novel treatments for human patients has forced some to identify ways to validate preclinical findings prior to initiating expensive and time-consuming clinical trials. Human tissue recovered from patients undergoing surgery and/o作者: 抒情短詩 時間: 2025-3-26 10:28
https://doi.org/10.1007/978-3-663-04972-2ll research landscape tremendously. Their discovery opened the unprecedented opportunity to work with human-induced pluripotent stem cells and the differentiated progeny thereof, without major ethical restrictions. Additionally, the new method offers the possibility to generate pluripotent stem cell作者: 大溝 時間: 2025-3-26 14:52 作者: enlist 時間: 2025-3-26 20:23 作者: 休閑 時間: 2025-3-26 23:23 作者: 細節(jié) 時間: 2025-3-27 01:58 作者: bypass 時間: 2025-3-27 08:09 作者: ostracize 時間: 2025-3-27 11:38
https://doi.org/10.1007/978-3-663-04975-3ffer significantly to the environments in intact animals. Also, traditional methods like patch clamp electrophysiology are very low throughput. In vivo calcium imaging of peripheral ganglia strives to overcome these limitations. In this chapter, we will discuss the development and procedures associa作者: Admire 時間: 2025-3-27 16:26 作者: Ossification 時間: 2025-3-27 19:38 作者: Chronic 時間: 2025-3-27 23:22 作者: 假設(shè) 時間: 2025-3-28 05:19
Wilhelm Sturtzel,Werner Graff,Klaus Suhrbier in the analysis of somatosensory spinal circuits as it allows for the local restriction of transgene expression and determination of the onset of expression. The combination of recombinase-dependent AAV vectors with transgenic mice enables cell type-specific expression of any protein, within the li作者: Foregery 時間: 2025-3-28 06:21
Rebecca P. SealIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: Adherent 時間: 2025-3-28 14:14 作者: irradicable 時間: 2025-3-28 17:27
In Vivo Calcium Imaging of Peripheral Ganglia,ffer significantly to the environments in intact animals. Also, traditional methods like patch clamp electrophysiology are very low throughput. In vivo calcium imaging of peripheral ganglia strives to overcome these limitations. In this chapter, we will discuss the development and procedures associated with this powerful new technique.作者: 贊成你 時間: 2025-3-28 20:15
https://doi.org/10.1007/978-3-663-04974-6Neural circuit tracing methods that take advantage of viral vectors and transgenic mice provide opportunities to gain insight into functional connectivity. Here we describe several protocols for identifying synaptic connections made by projection neurons, specific cell types, and neural ensembles.作者: 畢業(yè)典禮 時間: 2025-3-29 01:52
Using Viral Vectors to Visualize Pain-Related Neural Circuits in Mice,Neural circuit tracing methods that take advantage of viral vectors and transgenic mice provide opportunities to gain insight into functional connectivity. Here we describe several protocols for identifying synaptic connections made by projection neurons, specific cell types, and neural ensembles.作者: 倒轉(zhuǎn) 時間: 2025-3-29 06:49
0893-2336 etworks?.is a valuable resource for scientists and researchers interested in making impactful contributions to our understanding of pain..978-1-0716-2041-0978-1-0716-2039-7Series ISSN 0893-2336 Series E-ISSN 1940-6045 作者: Ledger 時間: 2025-3-29 10:20
Ex Vivo Skin-Teased Fiber Recordings from Tibial Nerve,作者: 馬賽克 時間: 2025-3-29 13:19
Single-Cell RNA Sequencing of Somatosensory Neurons,ally, we will highlight potential pitfalls and strategies when working with this sensory tissue, in particular spinal cord with its highly sensitive neurons. In summary, we present a comprehensive description on how to perform single-cell RNA-sequencing including suggestions on how to choose and des作者: cyanosis 時間: 2025-3-29 16:03 作者: 鑲嵌細工 時間: 2025-3-29 23:24 作者: 失敗主義者 時間: 2025-3-30 03:24 作者: Thyroiditis 時間: 2025-3-30 04:14 作者: CURT 時間: 2025-3-30 08:44
Recording Pain-Related Brain Activity in Behaving Animals Using Calcium Imaging and Miniature Microocol that uses recent advances in in vivo microscopy and computational techniques to address these questions. Miniaturized fluorescence microscopes (miniscopes) using microendoscopy allow for imaging of intracellular Ca. transients, which function as a proxy for neural activity. This innovative tech作者: duplicate 時間: 2025-3-30 15:18 作者: 低三下四之人 時間: 2025-3-30 18:28 作者: 注意到 時間: 2025-3-31 00:15 作者: Euthyroid 時間: 2025-3-31 03:07 作者: 確定方向 時間: 2025-3-31 07:17
,Die Passivierung von Wei?blech,many of these issues. An additional benefit of snRNA-seq is that it can be performed on frozen tissues, thus opening the door to the study of biobanked pathological human tissues. We present here a straightforward protocol to isolate both murine and human spinal cord nuclei for transcriptomic analys作者: sperse 時間: 2025-3-31 11:47 作者: 要求比…更好 時間: 2025-3-31 14:41
https://doi.org/10.1007/978-3-663-04972-2ertheless, the work with sensory-like neurons derived from human pluripotent stem cells has become a more widely used tool also in the field of pain research, as during the past years several differentiation procedures have been published that describe the generation of different types of sensory-li作者: 鋸齒狀 時間: 2025-3-31 18:57 作者: FISC 時間: 2025-3-31 21:47
https://doi.org/10.1007/978-3-663-04974-6ocol that uses recent advances in in vivo microscopy and computational techniques to address these questions. Miniaturized fluorescence microscopes (miniscopes) using microendoscopy allow for imaging of intracellular Ca. transients, which function as a proxy for neural activity. This innovative tech作者: FANG 時間: 2025-4-1 05:33
https://doi.org/10.1007/978-3-663-04976-0oped techniques utilizing optogenetic mouse models. These techniques will enable researchers to define how subtypes of afferent neurons and nonneuronal cell populations contribute to visceral pain in both normal and pathological conditions. This chapter details how optogenetics can be integrated int作者: 灰姑娘 時間: 2025-4-1 07:35 作者: 白楊 時間: 2025-4-1 10:26 作者: 斷言 時間: 2025-4-1 16:04
Single-Cell RNA Sequencing of Somatosensory Neurons,ns limited. However, single-cell transcriptomics has provided a completely new insight into the cellular complexity of sensory tissue. Thus, recent publications describe the transcriptome of spinal cord and dorsal root ganglia including the expression profile of cell types. Essential for successful 作者: 否認 時間: 2025-4-1 18:34
Preparation of Human and Rodent Spinal Cord Nuclei for Single-Nucleus Transcriptomic Analysis,ed as a powerful method to study heterogeneous tissues by allowing for the capture and analysis of individual cells. A key step in the analysis of solid tissues is the dissociation of the tissue into single cells. While many tissues can be readily dissociated by enzymatic or mechanical methods, high作者: 引起痛苦 時間: 2025-4-1 23:59 作者: cataract 時間: 2025-4-2 05:37
