標(biāo)題: Titlebook: Connexin Hemichannels; Methods and Protocol Fabio Mammano,Mauricio Retamal Book 2024 The Editor(s) (if applicable) and The Author(s), under [打印本頁] 作者: 板條箱 時(shí)間: 2025-3-21 18:32
書目名稱Connexin Hemichannels影響因子(影響力)
作者: GNAT 時(shí)間: 2025-3-21 22:37
Expression and Function of Connexins in Extracellular Vesicles,y explored for diagnosis and therapeutic purposes. The presence of multiple connexin (Cx) proteins has been described in EVs, where they might facilitate EV–cell communication. However, quantitative changes in Cx levels and functional assessment of Cx channels have only been established for Cx43. In作者: 命令變成大炮 時(shí)間: 2025-3-22 01:31
Expression, Purification, and Nanodisc Reconstitution of Connexin-43 Hemichannels for Structural Chterface of two neighboring cells. The HCs may be involved in ion and small-molecule transport across the cellular plasma membrane in response to various stimuli. Despite their importance, relatively few structures of connexin HCs are available to date, compared to the structures of the GJCs. Here, w作者: Pander 時(shí)間: 2025-3-22 05:31
Molecular Dynamics Simulation of Permeation Through Connexin Channels, level. They offer possibilities that are often unavailable to experimental methods, making MD an ideal complementary technique for the understanding a plethora of biological processes. Thanks to significant efforts by many groups of developers around the world, setting up and running MD simulations作者: Postulate 時(shí)間: 2025-3-22 10:16
Spatial and Temporal Localization of Connexins in Cells Using Confocal Microscopy,ing intercellular channels. These large-pore channels are dynamic and typically have a short life span of only a few hours. Imaging connexins from the time of synthesis in the endoplasmic reticulum through to their degradation can be challenging given their distinct assembly states and transient res作者: onlooker 時(shí)間: 2025-3-22 15:54 作者: onlooker 時(shí)間: 2025-3-22 17:11 作者: WAX 時(shí)間: 2025-3-22 21:24 作者: Constrain 時(shí)間: 2025-3-23 04:36
Evaluation of Connexin Hemichannel Activity In Vivo,ive flow of small hydrophilic molecules and ions (≤1?kDa) between the cytosol and the extracellular environment. Activation of Cx HCs is highly dependent on pathological conditions. HC activity provokes changes in the microenvironment, inducing the dissemination of signaling molecules in both an aut作者: 事與愿違 時(shí)間: 2025-3-23 08:46
Protocol for the Study of Connexin and DNA Interactions, of ions and molecules between the intra- and extracellular space and between cytoplasm of adjacent cells, respectively. The channel function of Cx assemblies has been extensively studied; however, “noncanonical” functions have emerged in the last few decades and have capture the attentions of many 作者: 射手座 時(shí)間: 2025-3-23 12:14
,Enhanced Methodologies for?Investigating the Electrophysiological Characteristics of Endogenous Panpotential distribution across cellular networks. The family of?Pannexin (Panx) proteins, in particular Pannexin1 (Panx1), are widely?expressed in vertebrate cells and exhibit sequence homology with innexins, the invertebrate gap junction channel?constituents. Despite being ubiquitously expressed, de作者: Tremor 時(shí)間: 2025-3-23 17:57 作者: 變形 時(shí)間: 2025-3-23 19:32
A Protocol for the Automated Assessment of Cutaneous Pathology in a Mouse Model of Hemichannel Dysfhyperactive connexin 26 hemichannels. Reflectance imaging is a versatile and powerful tool in dermatology, offering noninvasive, high-resolution insights into skin pathology, which is essential for both clinical practice and research. This approach offers several advantages and applications. Unlike 作者: 顯而易見 時(shí)間: 2025-3-23 23:13
Measuring Connexin Hemichannel Opening in Response to an InsP3-Mediated Cytosolic Ca2+ Increase,ncluding extracellular and intracellular Ca. ions. Submicromolar variations of the cytosolic Ca. concentration ([Ca.].) are per se sufficient to trigger extracellular bursts of messenger molecules through connexin HCs, thus mediating paracrine signaling. In this chapter, we present a quantitative me作者: ADORN 時(shí)間: 2025-3-24 03:57 作者: 紀(jì)念 時(shí)間: 2025-3-24 07:02
1064-3745 ation advice from the experts.This volume explores the latest developments in the study of connexin hemichannels (HCs). The chapters in this book cover topics such as purification, reconstitution, and functional analysis of connexin HCs; spatial and temporal localization of connexins in cells using 作者: Fabric 時(shí)間: 2025-3-24 11:49 作者: neoplasm 時(shí)間: 2025-3-24 17:03 作者: pester 時(shí)間: 2025-3-24 20:19 作者: 內(nèi)疚 時(shí)間: 2025-3-24 23:35
Mordechai Feingold,Victor Navarro-Brotonsd?cause deafness. Studies using purified isolated systems under well-controlled conditions are essential for a full understanding of molecular mechanisms of hemichannel function under normal conditions and in disease, and here, we present methodology for the expression, purification, and functional analysis of hemichannels formed by Cx26.作者: BLANK 時(shí)間: 2025-3-25 05:31
https://doi.org/10.1007/1-4020-4418-6ed with fluorescent proteins and when immunolabeled with high affinity anti-connexin antibodies in fixed cells. Temporal and spatial localization of multiple connexins and disease-linked connexin mutants at the subcellular level extensively informs on the mechanisms governing connexin regulation in health and disease.作者: 集聚成團(tuán) 時(shí)間: 2025-3-25 09:04 作者: Tracheotomy 時(shí)間: 2025-3-25 13:23
Uncertainty, Rationality, and Agencyion of Cx46 with DNA in HeLa cells. These methods can facilitate understanding the role of Cxs in physiological processes and pathological mechanisms, including, for example, the contribution of Cx46 in maintaining stemness of glioma cancer stem cells.作者: 慢跑鞋 時(shí)間: 2025-3-25 19:49 作者: Bernstein-test 時(shí)間: 2025-3-25 21:44
Spatial and Temporal Localization of Connexins in Cells Using Confocal Microscopy,ed with fluorescent proteins and when immunolabeled with high affinity anti-connexin antibodies in fixed cells. Temporal and spatial localization of multiple connexins and disease-linked connexin mutants at the subcellular level extensively informs on the mechanisms governing connexin regulation in health and disease.作者: 細(xì)頸瓶 時(shí)間: 2025-3-26 02:53 作者: 滋養(yǎng) 時(shí)間: 2025-3-26 07:25 作者: amenity 時(shí)間: 2025-3-26 08:32 作者: 縱火 時(shí)間: 2025-3-26 12:45 作者: 反叛者 時(shí)間: 2025-3-26 18:11 作者: 潰爛 時(shí)間: 2025-3-27 00:47 作者: LIMN 時(shí)間: 2025-3-27 03:27
Frank A. G. den Butter,Marjan W. Hofkesracellular and intracellular regions are quite different. In this chapter, we provide a detailed instruction to perform MD simulations aimed at computationally modeling the permeation of inorganic ions and larger molecules through Cx HCs.作者: 遺產(chǎn) 時(shí)間: 2025-3-27 06:15
https://doi.org/10.1007/1-4020-4631-6ls, a human oligodendroglioma cell line that naturally expresses Panx1. We anticipate these?refined protocols will significantly contribute?to future characterizations of Panx1-based intercellular?cell–cell channels?across diverse cell types and?offer valuable insights into both normal?cellular physiology and pathophysiology.作者: 表示問 時(shí)間: 2025-3-27 09:42
Yu.H. Israfilov,M.A. Asadov,T.M. Rashidov contribution to understanding the structure–function relationship of connexin HCs. The protocol is also suitable to screen candidate therapeutic compounds to treat connexin-related diseases linked to HC dysfunction.作者: CROAK 時(shí)間: 2025-3-27 15:11 作者: 坦白 時(shí)間: 2025-3-27 18:08 作者: BROOK 時(shí)間: 2025-3-28 00:02
,Enhanced Methodologies for?Investigating the Electrophysiological Characteristics of Endogenous Panls, a human oligodendroglioma cell line that naturally expresses Panx1. We anticipate these?refined protocols will significantly contribute?to future characterizations of Panx1-based intercellular?cell–cell channels?across diverse cell types and?offer valuable insights into both normal?cellular physiology and pathophysiology.作者: 自戀 時(shí)間: 2025-3-28 05:50 作者: 留戀 時(shí)間: 2025-3-28 06:20
Book 2024iding known pitfalls..Cutting-edge and comprehensive, .Connexin Hemichannels: Methods and Protocols. is a valuable resource that discusses the importance of HCs as drug targets, and how studying them is essential to identifying newcompounds capable of modulating HC function selectively and specifically.. . .作者: 吸引力 時(shí)間: 2025-3-28 10:31
Fabio Mammano,Mauricio RetamalIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: inspired 時(shí)間: 2025-3-28 16:11 作者: 征服 時(shí)間: 2025-3-28 20:26
https://doi.org/10.1007/978-1-0716-3842-2membrane proteins; bacteria; exosomes; cellular physiology; ATP release作者: invert 時(shí)間: 2025-3-28 23:19 作者: CLASH 時(shí)間: 2025-3-29 07:02
Mordechai Feingold,Victor Navarro-Brotons-head docking of hemichannels (each from one of two adjacent cells). Free “undocked” hemichannels at the plasma membrane are mostly closed, although they are still important under physiological conditions. However, abnormal and sustained increase in hemichannel activity due to connexin mutations or 作者: Salivary-Gland 時(shí)間: 2025-3-29 10:02 作者: 紀(jì)念 時(shí)間: 2025-3-29 13:11 作者: 在前面 時(shí)間: 2025-3-29 16:24
Frank A. G. den Butter,Marjan W. Hofkes level. They offer possibilities that are often unavailable to experimental methods, making MD an ideal complementary technique for the understanding a plethora of biological processes. Thanks to significant efforts by many groups of developers around the world, setting up and running MD simulations作者: 沒收 時(shí)間: 2025-3-29 19:46
https://doi.org/10.1007/1-4020-4418-6ing intercellular channels. These large-pore channels are dynamic and typically have a short life span of only a few hours. Imaging connexins from the time of synthesis in the endoplasmic reticulum through to their degradation can be challenging given their distinct assembly states and transient res作者: Ardent 時(shí)間: 2025-3-30 03:50
Roque Corral,Nélida Cerezal,Cárlos Vasco support intercellular communication mechanisms and regulate homeostasis. In contrast, open connexin hemichannels connect the intracellular compartment and the extracellular environment, and their activation fuels inflammation and cell death. The development of clinically applicable connexin hemicha作者: 繁忙 時(shí)間: 2025-3-30 07:29
Junichi Kazawa,Toshinori Watanabey several protein families with little or no evolutionary linkages that include connexins (Cxs), pannexins (Panxs), innexins (Inxs), CALHM1, and LRRC8 proteins. Recently, we have described the unnexins (Unxs) proteins expressed in . (.) that also is like to form large-pore channels at the plasma mem作者: overture 時(shí)間: 2025-3-30 10:17
https://doi.org/10.1007/1-4020-4605-7in-of-function etiopathogenetic mechanism for several diseases, ranging from skin disorders to nervous system defects. Furthermore, the opening of nonmutated Cx HCs is associated with an impressive list of widespread diseases including, but not limited to, ischemia/stroke, Alzheimer’s disease, and e作者: Lacerate 時(shí)間: 2025-3-30 14:21
https://doi.org/10.1007/1-4020-4605-7ive flow of small hydrophilic molecules and ions (≤1?kDa) between the cytosol and the extracellular environment. Activation of Cx HCs is highly dependent on pathological conditions. HC activity provokes changes in the microenvironment, inducing the dissemination of signaling molecules in both an aut作者: moribund 時(shí)間: 2025-3-30 18:20 作者: JADED 時(shí)間: 2025-3-30 22:54 作者: 使高興 時(shí)間: 2025-3-31 03:59
URBAN GROUNDWATER POLLUTION IN TURKEYent expression from a plasmid transfection. Stably expressing a transgene of interest in cells under induction is a powerful way to (switch on and) study a gene function in both in vitro and in vivo assays. Taking advantage of the ability of lentivirus (LV) to promote transgene delivery, and genomic
