標題: Titlebook: Confocal Microscopy; Methods and Protocol Joseph Brzostowski,Haewon Sohn Book 2021 This is a U.S. government work and not under copyright p [打印本頁] 作者: 我贊成 時間: 2025-3-21 17:27
書目名稱Confocal Microscopy影響因子(影響力)
書目名稱Confocal Microscopy影響因子(影響力)學科排名
書目名稱Confocal Microscopy網(wǎng)絡(luò)公開度
書目名稱Confocal Microscopy網(wǎng)絡(luò)公開度學科排名
書目名稱Confocal Microscopy被引頻次
書目名稱Confocal Microscopy被引頻次學科排名
書目名稱Confocal Microscopy年度引用
書目名稱Confocal Microscopy年度引用學科排名
書目名稱Confocal Microscopy讀者反饋
書目名稱Confocal Microscopy讀者反饋學科排名
作者: LAP 時間: 2025-3-21 22:59
Joseph Brzostowski,Haewon SohnIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: Handedness 時間: 2025-3-22 02:03 作者: 狗舍 時間: 2025-3-22 06:37
Fluorescent biochemical sensors allow probing metabolic states in a living cell with high spatiotemporal dynamics. This chapter describes a method for the in situ detection of changes in NAD. level in living cells using fluorescence lifetime imaging (FLIM).作者: 精美食品 時間: 2025-3-22 10:59
FLIM Imaging for Metabolic Studies in Live Cells,Fluorescent biochemical sensors allow probing metabolic states in a living cell with high spatiotemporal dynamics. This chapter describes a method for the in situ detection of changes in NAD. level in living cells using fluorescence lifetime imaging (FLIM).作者: Defense 時間: 2025-3-22 14:47 作者: Defense 時間: 2025-3-22 17:58
978-1-0716-1404-4This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright pro作者: STIT 時間: 2025-3-22 21:53
and widespread accessibility of confocal microscopy ensure that it will have a prominent place in biological imaging for many years to come, even with the recent advances in light sheet and field synthesis microscopy. Since these more advanced technologies still require significant expertise to effe作者: Benzodiazepines 時間: 2025-3-23 02:20 作者: 揉雜 時間: 2025-3-23 08:43 作者: incubus 時間: 2025-3-23 12:34 作者: HAVOC 時間: 2025-3-23 15:16 作者: 哀悼 時間: 2025-3-23 20:15 作者: 宴會 時間: 2025-3-23 23:38
expansion is uniform across observable length scales, enabling imaging of structures previously too small to resolve. ExM is compatible with any microscope and does not require expensive materials or specialized software, offering effectively sub-diffraction-limited imaging capabilities to labs that作者: brachial-plexus 時間: 2025-3-24 04:33 作者: 尖酸一點 時間: 2025-3-24 10:31
the exocytic vesicle networks, shuttle material in and out the cell, respectively. The?substantial development of cell biological imaging techniques, along with improved fluorescent probes and image analysis tools, has been instrumental in increasing our understanding of various functions and regul作者: Esalate 時間: 2025-3-24 12:23 作者: acrimony 時間: 2025-3-24 16:56
a well-established model to study both processes. Recent studies show that a G-protein-coupled receptor (fAR1) mediate a signaling network to control reorganization of the actin cytoskeleton leading both the directional cell movement and the engulfment of bacteria. Many live cell imaging methods ha作者: abracadabra 時間: 2025-3-24 19:55 作者: paradigm 時間: 2025-3-25 02:23
es that directly or indirectly compromise cytoskeletal stability. While the large size and complexity of the neurons grown in culture poses certain challenges for imaging, live-cell imaging is an excellent approach to determine the morphological consequences of such mutants. This protocol details th作者: 珠寶 時間: 2025-3-25 06:11 作者: 維持 時間: 2025-3-25 11:17
ning to the ground state. It is a state-of-the-art and noninvasive technique that has the potential to obtain signature physiological information during malaria blood-stage infection. The use of autofluorescence signals from intrinsic fluorophores obviates the need to tag the cells with synthetic mo作者: 懸掛 時間: 2025-3-25 14:29 作者: Coordinate 時間: 2025-3-25 19:39 作者: Insubordinate 時間: 2025-3-25 20:04 作者: 持續(xù) 時間: 2025-3-26 02:25
High-Resolution Multicolor Imaging of Mitochondria in Lymphocytes,e use of Mitotracker dyes) while efficiently excluding nonviable cells. Our novel imaging strategy offers a powerful tool to study changes in mitochondrial morphology and complements any research focusing on lymphocyte metabolism.作者: 套索 時間: 2025-3-26 04:40 作者: 排出 時間: 2025-3-26 09:26 作者: 鎮(zhèn)壓 時間: 2025-3-26 16:00 作者: 臭了生氣 時間: 2025-3-26 17:29
,Visualizing the Dynamics of T Cell–Dendritic Cell Interactions in Intact Lymph Nodes by Multiphoton作者: Insufficient 時間: 2025-3-27 00:07
1064-3745 ding known pitfalls..?..Authoritative and cutting-edge,?.Confocal Microscopy: Methods and Protocols .aims to ensure successful results in the further study of this vital field..978-1-0716-1404-4978-1-0716-1402-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: FIG 時間: 2025-3-27 04:58
tional confocal function, the Airyscan unit comes as an add-on to the conventional Zeiss laser scanning confocal microscope. This protocol is for the first generation Airyscan Zeiss 800 series microscope.作者: jungle 時間: 2025-3-27 08:38
e use of Mitotracker dyes) while efficiently excluding nonviable cells. Our novel imaging strategy offers a powerful tool to study changes in mitochondrial morphology and complements any research focusing on lymphocyte metabolism.作者: 歡呼 時間: 2025-3-27 12:11
these cells. Lastly, we describe two types of quantitative analysis: vesicle distribution/clustering toward?the microtubule organizing center (MTOC), and colocalization analysis with endolysosomal markers.作者: 大罵 時間: 2025-3-27 14:38
.infected RBCs. The data shared with this protocol reveals that there is a significant overall increase in autofluorescence lifetime in infected erythrocytes compared to the healthy uninfected ones. We include a metabolic experiment that confirms that the signals obtained from this imaging technique作者: 使糾纏 時間: 2025-3-27 19:32 作者: DEFER 時間: 2025-3-27 23:17 作者: Ebct207 時間: 2025-3-28 05:20
Choosing Fluorescent Probes and Labeling Systems,s when designing an experiment is the selection of an appropriate fluorescent probe. Equally important is deciding how the probe will be attached to the protein of interest. The advantages and disadvantages of different fluorescent probe types and their respective labeling methods are discussed to p作者: ostracize 時間: 2025-3-28 07:49 作者: Antigen 時間: 2025-3-28 13:01
Microfabricated Devices for Confocal Microscopy on Biological Samples,d and multiple commercial devices now available. This chapter is intended to provide an overview of the available options for devices compatible with confocal microscopy, including an overview of fabrication techniques and some examples of device use. Although there are times when off-the-shelf devi作者: 使成整體 時間: 2025-3-28 17:40
ZEISS Airyscan: Optimizing Usage for Fast, Gentle, Super-Resolution Imaging,zed 32-channel Airyscan detector. By improving resolution twofold and signal-to-noise ratio eightfold relative to conventional confocal microscopes while retaining confocal functionality, the Airyscan microscope has become a very popular super-resolution imaging tool for cell biologists. In this cha作者: 放氣 時間: 2025-3-28 22:17 作者: 脫毛 時間: 2025-3-29 01:57
Protein-Retention Expansion Microscopy (ExM): Scalable and Convenient Super-Resolution Microscopy,expansion is uniform across observable length scales, enabling imaging of structures previously too small to resolve. ExM is compatible with any microscope and does not require expensive materials or specialized software, offering effectively sub-diffraction-limited imaging capabilities to labs that作者: 改進 時間: 2025-3-29 03:38 作者: 小畫像 時間: 2025-3-29 09:06 作者: 新星 時間: 2025-3-29 14:01
Visualizing Key Signaling Components of Macropinocytosis and Phagocytosis Using Confocal Microscopyrise to an internal compartment called the macropinosomes or phagosome, respectively. . provides a powerful system to understand the molecular mechanism of these two fundamental cellular processes that impact human health and disease. Recent developments in fluorescence microscopy allow direct visua作者: 警告 時間: 2025-3-29 19:20
Imaging GPCR-Mediated Signal Events Leading to Chemotaxis and Phagocytosis, a well-established model to study both processes. Recent studies show that a G-protein-coupled receptor (fAR1) mediate a signaling network to control reorganization of the actin cytoskeleton leading both the directional cell movement and the engulfment of bacteria. Many live cell imaging methods ha作者: eulogize 時間: 2025-3-29 22:48
High-Throughput Imaging of Arrays of Fluorescently Tagged Yeast Mutant Strains,artments of choice using automated confocal microscopy. This procedure, which combines HTP genetics and microscopy, results in the acquisition of thousands of images that can be analyzed in a systematic and quantitative way to identify morphology defects in the tagged subcellular compartments. This 作者: ingenue 時間: 2025-3-30 03:39
Studying Neuronal Biology Using Spinning Disc Confocal Microscopy,es that directly or indirectly compromise cytoskeletal stability. While the large size and complexity of the neurons grown in culture poses certain challenges for imaging, live-cell imaging is an excellent approach to determine the morphological consequences of such mutants. This protocol details th作者: 一罵死割除 時間: 2025-3-30 06:56 作者: delusion 時間: 2025-3-30 10:25 作者: 蚊帳 時間: 2025-3-30 14:00 作者: 機警 時間: 2025-3-30 18:15
A Step-by-Step Guide to Instant Structured Illumination Microscopy (iSIM),ng twice the diffraction limit. Here we briefly describe the theory of iSIM and outline a typical hardware setup. We also provide step-by-step guides for generating a cellular-based fluorescent standard, obtaining a multicolor image with iSIM, and the post-processing steps of de-striping and deconvo作者: 樂意 時間: 2025-3-30 20:51
1064-3745 ation advice from the experts.This volume provides a wide range of imaging protocols that can be tailored to specific organisms or cell-types.? Chapters guide readers through fixed-cell, live-cell, phenotype screening, super-resolution, intravital imaging techniques, and fluorescence life-time imagi作者: Catheter 時間: 2025-3-31 03:54 作者: EVADE 時間: 2025-3-31 08:03
opy. This basic protocol steps the user through a typical three-color imaging experiment using a Zeiss LSM 880 confocal microscope for the example. The extensive Notes section attempts to generalize the method so that concepts and considerations can be applied to other laser scanning confocal systems.作者: 伙伴 時間: 2025-3-31 12:50
reorganization of the actin cytoskeleton leading both the directional cell movement and the engulfment of bacteria. Many live cell imaging methods have been developed and applied to monitor these signaling events. In this chapter, we will introduce how to measure GPCR-mediated signaling events for cell migration and phagocytosis in ..作者: 整潔漂亮 時間: 2025-3-31 15:08 作者: manifestation 時間: 2025-3-31 18:03
Choosing Fluorescent Probes and Labeling Systems,he protein of interest. The advantages and disadvantages of different fluorescent probe types and their respective labeling methods are discussed to provide an overview on selecting appropriate fluorophores and labeling systems for fluorescence-based assays. Protocols are outlined when appropriate.作者: 強制令 時間: 2025-4-1 00:58
General Considerations for Acquiring a Three-Color Image by Laser Scanning Confocal Microscopy,opy. This basic protocol steps the user through a typical three-color imaging experiment using a Zeiss LSM 880 confocal microscope for the example. The extensive Notes section attempts to generalize the method so that concepts and considerations can be applied to other laser scanning confocal systems.作者: 不來 時間: 2025-4-1 03:20 作者: 聯(lián)合 時間: 2025-4-1 07:00
High-Throughput Imaging of Arrays of Fluorescently Tagged Yeast Mutant Strains,sands of images that can be analyzed in a systematic and quantitative way to identify morphology defects in the tagged subcellular compartments. This HTP protocol is readily adapted for screening any combination of markers and can be expanded to different growth conditions or higher order mutant genetic backgrounds.作者: Infantry 時間: 2025-4-1 14:00 作者: 銀版照相 時間: 2025-4-1 15:13 作者: 保守黨 時間: 2025-4-1 20:48
e use of spinning disk confocal microscopy and image analysis tools to evaluate branching and neurite length of healthy iPSC-derived glutamatergic neurons that express specific fluorescent proteins. The protocols can be adapted to neuronal cell lines of choice by the investigator.作者: Vasodilation 時間: 2025-4-2 02:20
