作者: 小歌劇 時(shí)間: 2025-3-21 23:47
Smart Learning with Educational Roboticsxpression systems have been developed in order to construct such libraries containing tens to hundreds of mlllions of random peptlde sequences that can be screened for their abihty to bind particular antibodies (.). In essence these phage-expressed peptides mimic the determinants of the antigen that are recognized by the antibodies.作者: DIS 時(shí)間: 2025-3-22 02:42 作者: intelligible 時(shí)間: 2025-3-22 07:06 作者: LATHE 時(shí)間: 2025-3-22 10:19
https://doi.org/10.1007/978-3-031-54207-7 same compound in and on the same bead (.–.). With an appropriate detection scheme, compound-beads with specific biological, physical, or chemical properties can be identified, and physically isolated, and then their chemical structure can be determined. In biological systems, one important property作者: Esophagus 時(shí)間: 2025-3-22 14:18
https://doi.org/10.1007/978-3-031-54207-7ing blocks used are represented. The development and verification of the utility of combinatorial libraries represent a dramatic advance in the drug discovery process by greatly reducing the time needed to identify new drug leads. Positional scanning (PS) SCLs (.,.) represent a modified format of th作者: Esophagus 時(shí)間: 2025-3-22 20:44
https://doi.org/10.1007/978-3-031-54207-7 detection of epitopes as well as for the identification of peptides that act as antagonists of medically relevant proteins. We have prepared different types of cellulose-bound peptide libraries by spot synthesis (.), which is a powerful tool for the simultaneous and positionally addressable synthes作者: 無力更進(jìn) 時(shí)間: 2025-3-22 22:01 作者: isotope 時(shí)間: 2025-3-23 03:47 作者: 失敗主義者 時(shí)間: 2025-3-23 07:16
Authoring Tools to Design Smart LOscommercially available bis-2-aminopropyl-PEG.. With the use of this PEG., permeability with proteins up to 50 kDa has been achieved, as demonstrated by gel permeation chromatography (.). A resin-bound fluoroescencequenched peptide substrate showed 80% cleavage with subtihsin Carlsberg (MW 27 kDa) in作者: Mendicant 時(shí)間: 2025-3-23 12:59 作者: laxative 時(shí)間: 2025-3-23 17:19 作者: 沒有準(zhǔn)備 時(shí)間: 2025-3-23 19:54
Diana Tavares,Sandra Mota,Manuela Amorimlation sites reported (.), and many protein kinases have been cloned and expressed. The sites of phosphorylation are usually serine, threonine, tyrosine, or occastonally histrdine. The two major classes of protein kinases are serme-threonine protein kmase and protein tyrosine kinase. Substrate motif作者: Transfusion 時(shí)間: 2025-3-24 00:22 作者: 悄悄移動 時(shí)間: 2025-3-24 04:26
Smart Learning in Classroom Environmentnases exhibit specificities that are often primarily determined by the amino acrds around the phosphorylation sites (.). Identification of amino acids that contribute to substrate motifs are essential for the understanding of signal transduction pathways and for the development of specific peptide s作者: Malfunction 時(shí)間: 2025-3-24 08:07
Contexts of Smart Learning Environmentsupporting matrix in a controlled manner so that fractions are available for multiple independent tests, free of interference from other constituents of the library. A method was developed to study the functional responses arlsing from individual constituent beads in a synthetic combinatorial peptide作者: disparage 時(shí)間: 2025-3-24 12:18 作者: Incumbent 時(shí)間: 2025-3-24 15:36
https://doi.org/10.1007/978-981-10-4343-7f1,fd,and M13, all of which Infect . cells through their F pill. This group of F-spectfic phage share 98% homology in their DNA sequence. Their nme coding genes (coding for ten proteins), as well as then two mtergenic regions, are ordered and oriented in the same way. The phage particle has a filame作者: 我不重要 時(shí)間: 2025-3-24 19:30 作者: Nefarious 時(shí)間: 2025-3-24 23:42 作者: 間接 時(shí)間: 2025-3-25 04:44 作者: Adenocarcinoma 時(shí)間: 2025-3-25 10:37 作者: GIBE 時(shí)間: 2025-3-25 12:19
Construction and Use of a 20-mer Phage Display Epitope Library,xpression systems have been developed in order to construct such libraries containing tens to hundreds of mlllions of random peptlde sequences that can be screened for their abihty to bind particular antibodies (.). In essence these phage-expressed peptides mimic the determinants of the antigen that are recognized by the antibodies.作者: 光亮 時(shí)間: 2025-3-25 18:27 作者: avulsion 時(shí)間: 2025-3-25 21:08
Kusum Lata,Priyanka Kumar,Arpita Banerjeenique for screening combinatorial libraries employing recombinant receptors expressed in the plasma membrane of transfected melanophors (.,. and Chapters 13,14). Their technique is based on the biological activity of the expressed receptors and it enables the selection of library members with agonistic or antagonistic activities.作者: 頂點(diǎn) 時(shí)間: 2025-3-26 01:38
Peralkylation,CLs used as starting materials. The screening of a SCL composed of 50 million peptidomimetics (.) has yielded individual active compounds that had no homology to those found in the starting SCLs. We describe here improved methods (.) developed for the transformation of such libraries.作者: Asymptomatic 時(shí)間: 2025-3-26 05:19 作者: Exaggerate 時(shí)間: 2025-3-26 09:58
Synthesis and Screening of Peptide Libraries on Continuous Cellulose Membrane Supports,is of thousands of peptrdes or peptide mixtures bound to continuous cellulose membrane supports. Presently up to 8000 different spots (peptides or peptide mixtures) can be automatically synthesized on a 20 *x 30-cm cellulose membrane and screened for ligand binding within l–2 wk.作者: Fortuitous 時(shí)間: 2025-3-26 14:25
Construction of Disulfide-Constrained Random Peptide Libraries Displayed on Phage Coat Protein VIIIing both recombinant and wild-type proteins. The high copy number of recombinant pVIII molecules per phage particle provides a highly sensitive system, and linear and constrained peptide libraries in pVIII have been successfully used for the selection of specific ligands for several different monoclonal and polyclonal antibodies (.).作者: Omnipotent 時(shí)間: 2025-3-26 17:42
1064-3745 ving organisms, such as bacteria and viruses. This imbalance has given the latter the advantage of generating, relatively quickly, molecules with unexpected structures and features that carry a threat to vertebrates. To compensate for their weakness, vertebrates have accelerated their own evolutiona作者: ectropion 時(shí)間: 2025-3-26 23:49 作者: SMART 時(shí)間: 2025-3-27 01:27 作者: Notorious 時(shí)間: 2025-3-27 06:59 作者: 胖人手藝好 時(shí)間: 2025-3-27 13:31 作者: 獨(dú)行者 時(shí)間: 2025-3-27 13:37 作者: 共同給與 時(shí)間: 2025-3-27 21:04 作者: ineffectual 時(shí)間: 2025-3-27 22:01
The Solid-Phase Enzyme Inhibitor Library Assay,tial inhibitors remained dark while enzyme cleaved the substrate in beads containing noninhibitors, resulting in a dramatic increase in fluorescence. The dark beads can be collected and analyzed by sequence analysis.作者: Concomitant 時(shí)間: 2025-3-28 04:12 作者: 我要沮喪 時(shí)間: 2025-3-28 08:56 作者: Anonymous 時(shí)間: 2025-3-28 12:35
Linda Daniela,Raimonds Strods,Ilze Franceing both recombinant and wild-type proteins. The high copy number of recombinant pVIII molecules per phage particle provides a highly sensitive system, and linear and constrained peptide libraries in pVIII have been successfully used for the selection of specific ligands for several different monoclonal and polyclonal antibodies (.).作者: humectant 時(shí)間: 2025-3-28 17:50 作者: Lament 時(shí)間: 2025-3-28 19:28 作者: AFFIX 時(shí)間: 2025-3-29 01:19
Sokly Eav,Chawalit Jeenanunta,Yau Yan Wongy conserved, suggesting a common ancestor for these kinases (.) However, different kinases have evolved to function distinctly in response to diverse cellular stimuli. The specificities of protein kinases has thus become a critical issue in understanding signal transduction.作者: 雜色 時(shí)間: 2025-3-29 07:02
Smart Learning in Classroom Environmentas well as mhrbrtors (.). Peptide libraries offer the possibility to investigate the sequence dependence of the phosphorylation more thoroughly and systematically and may even allow the . delineation of peptide substrates of uncharacterized protein kinases.作者: 鬼魂 時(shí)間: 2025-3-29 09:38 作者: Bother 時(shí)間: 2025-3-29 14:01
The Use of Peptide Library for the Determination of Kinase Peptide Substrates,y conserved, suggesting a common ancestor for these kinases (.) However, different kinases have evolved to function distinctly in response to diverse cellular stimuli. The specificities of protein kinases has thus become a critical issue in understanding signal transduction.作者: VEST 時(shí)間: 2025-3-29 16:55 作者: aviator 時(shí)間: 2025-3-29 23:35 作者: 不可磨滅 時(shí)間: 2025-3-30 03:39 作者: 寬容 時(shí)間: 2025-3-30 06:47 作者: Innovative 時(shí)間: 2025-3-30 11:57 作者: 引起痛苦 時(shí)間: 2025-3-30 14:35 作者: 劇本 時(shí)間: 2025-3-30 18:28
Introduction to Combinatorial Solid-Phase Assays for Enzyme Activity and Inhibition,ic enzymes has been expressed by the pharmaceutical industry, and the investment in the development of specific enzyme mhlbltors remains an important challenge. This chapter describes combinatorial approaches to the characterization of enzyme specificity and identification of enzyme inhibitors or le作者: 背景 時(shí)間: 2025-3-30 21:24 作者: 捏造 時(shí)間: 2025-3-31 01:42 作者: altruism 時(shí)間: 2025-3-31 05:33 作者: 溝通 時(shí)間: 2025-3-31 10:54
,Determination of Peptide Substrate Motifs for Protein Kinases Using a “One-Bead One-Compound” Combilation sites reported (.), and many protein kinases have been cloned and expressed. The sites of phosphorylation are usually serine, threonine, tyrosine, or occastonally histrdine. The two major classes of protein kinases are serme-threonine protein kmase and protein tyrosine kinase. Substrate motif作者: Bother 時(shí)間: 2025-3-31 17:13 作者: lipids 時(shí)間: 2025-3-31 18:37
Analysis of Protein Kinase Substrate Specificity by the Use of Peptide Libraries on Cellulose Papernases exhibit specificities that are often primarily determined by the amino acrds around the phosphorylation sites (.). Identification of amino acids that contribute to substrate motifs are essential for the understanding of signal transduction pathways and for the development of specific peptide s作者: 承認(rèn) 時(shí)間: 2025-3-31 22:34
Generation of Multiuse Peptide Libraries for Functional Screenings,upporting matrix in a controlled manner so that fractions are available for multiple independent tests, free of interference from other constituents of the library. A method was developed to study the functional responses arlsing from individual constituent beads in a synthetic combinatorial peptide作者: Chemotherapy 時(shí)間: 2025-4-1 03:52
Functional Screening of Multiuse Peptide Libraries Using Melanophore Bioassay,ry (MUPL). In the melanophores, a cell hne derived from . skin, pigment dispersion can be effected via actrvation of adenyl cyclase (.,.) or phospholipase C (.), while pigment aggregation results from inhibition of adenyl cyclase (.,.). It has been shown that melanophore cells contain a wide range o作者: 無關(guān)緊要 時(shí)間: 2025-4-1 09:33
The Basic Structure of Filamentous Phage and Its Use in the Display of Combinatorial Peptide Librarf1,fd,and M13, all of which Infect . cells through their F pill. This group of F-spectfic phage share 98% homology in their DNA sequence. Their nme coding genes (coding for ten proteins), as well as then two mtergenic regions, are ordered and oriented in the same way. The phage particle has a filame
