作者: 消息靈通 時(shí)間: 2025-3-21 21:21
A Brief Historical Account of the Family , taxon comprising plant viruses that possess very long helically constructed filamentous particles and a positive-sense single-stranded, monopartite or bipartite RNA genome and are transmitted either by aphids (genus .), pseudococcid mealybugs/soft scale insects (genus .), or whiteflies (genus .) or作者: 彈藥 時(shí)間: 2025-3-22 00:33
Phenotyping Biological Properties of CTV Isolates,ogical properties of a single isolate of . complex (CTV). It enables the phenotypic profile of the isolates to be defined and to infer the associated tristeza diseases (decline, seedling yellows, or stem pitting), to assess their aggressiveness or potential cross protectiveness (if any), and to moni作者: fructose 時(shí)間: 2025-3-22 07:26 作者: 逢迎春日 時(shí)間: 2025-3-22 09:09
Tissue-Print and Squash Capture Real-Time RT-PCR Method for Direct Detection of , (CTV) in Plant orto polymerase chain reaction (RT-PCR) neither with extract preparation nor nucleic acid purification. Immobilized CTV targets are amplified from fresh sections of plant tissues or squashes of fresh or already caught vectors onto paper, nitrocellulose, or positively charged nylon membranes. The print作者: 含鐵 時(shí)間: 2025-3-22 13:12
Detection of , and Coinfecting Viroids, programs require pathogen detection systems which must be economical and sensitive to maintain a healthy citrus industry. Rapid diagnostic tests for simultaneous detection of major graft-transmissible disease agents enable reduction of cost and time. The genetic and biological features of viruses a作者: 含鐵 時(shí)間: 2025-3-22 20:00
Assessment of Genetic Variability of , by SSCP and CE-SSCP,tes. It is a simple, low-cost, and highly specific method for mutation detection of specific genes, mostly of the CTV major coat protein gene (.). The technique is based on a comparison on polyacrylamide gel of electrophoretic profiles of single-stranded (ss) DNA sequences in terms of their spatial 作者: HEPA-filter 時(shí)間: 2025-3-22 21:36 作者: Inordinate 時(shí)間: 2025-3-23 05:16 作者: 颶風(fēng) 時(shí)間: 2025-3-23 06:56
Rapid and Sensitive Detection of , Using Reverse Transcription Loop-Mediated Isothermal Amplificatil for early detection of nucleic acid targets. The LAMP technique works on the principle of strand displacement activity of . polymerase. It contains a set of four specially designed primers, which recognizes six different regions on the target nucleotide sequence. In the LAMP reaction, amplificatio作者: RODE 時(shí)間: 2025-3-23 13:22 作者: 幻想 時(shí)間: 2025-3-23 16:52
Bioinformatic Tools and Genome Analysis of ,and the study of host-pathogen interactions. Virus detection has been an important application of this technology, which has resulted in the discovery of novel viruses or viral strains as well as for the detection of known?viruses in a plant sample. Here we describe the entire process that needs to 作者: Nutrient 時(shí)間: 2025-3-23 21:27 作者: 重畫(huà)只能放棄 時(shí)間: 2025-3-24 01:23
: Host RNA Silencing and Virus Counteraction,d by the virus via its three RNA silencing suppressors (RSS), the small RNAs (sRNAs) of three virus-host combinations were deep sequenced. The subsequent analysis indicated that CTV sRNAs (1) constitute more than half of the total sRNAs in the susceptible Mexican lime and sweet orange, while only 3.作者: 下邊深陷 時(shí)間: 2025-3-24 05:39
Proteomic Response of Host Plants to ,roteins to obtain two-dimensional gels with a large number of spots, well distributed, and with good quality. The extraction process that associates successive washes with ultrasonication steps proved to be efficient for the detection of proteins. With this technique it was possible to obtain clear 作者: 宣傳 時(shí)間: 2025-3-24 07:37
,Gene Expression in Citrus Plant Cells Using Helios? Gene Gun System for Particle Bombardment,teins. However, due to technical limitations, so far these studies have been limited to the nonnatural host ...Particle bombardment is a physical method to deliver nucleic acid and other biomolecules into the cells directly. The Helios. gene gun (Bio-Rad, Hercules, CA) is a handheld device that uses作者: ELUDE 時(shí)間: 2025-3-24 14:19 作者: aplomb 時(shí)間: 2025-3-24 16:26
Book 2019, a member of the genus .Closterovirus., family .Closteroviridae.. Thanks to the highly sensitive and specific diagnostic procedures developed, knowledge of the molecular characteristics, expression strategies, genetic variability, and epidemiology of the virus has improved significantly, as this vo作者: 莎草 時(shí)間: 2025-3-24 21:49 作者: SPALL 時(shí)間: 2025-3-24 23:51 作者: 奴才 時(shí)間: 2025-3-25 06:28
Detection of , and Coinfecting Viroids,t can allow to selectively discriminate severe strain(s) is a powerful tool to intercept the most harmful strains and to reduce the need for biological indexing. Moreover a combination of these detection methods will facilitate the studies on the interactions between CTV and viroids, a research topic only partially explored so far.作者: Wordlist 時(shí)間: 2025-3-25 11:10 作者: electrolyte 時(shí)間: 2025-3-25 14:01 作者: 美色花錢(qián) 時(shí)間: 2025-3-25 19:37 作者: Common-Migraine 時(shí)間: 2025-3-25 22:24 作者: 損壞 時(shí)間: 2025-3-26 03:10
Kai-Yuan Cai,Chuan-Yuan Wen,Ming-Lian Zhang of novel viruses or viral strains as well as for the detection of known?viruses in a plant sample. Here we describe the entire process that needs to be considered for the genome analysis of . (CTV) by HTS, including the experimental design, sample preparation, nucleic acid purification, HTS library construction, and bioinformatic analysis.作者: 他去就結(jié)束 時(shí)間: 2025-3-26 05:13 作者: 藕床生厭倦 時(shí)間: 2025-3-26 10:53
Phenotyping Biological Properties of CTV Isolates,tristeza diseases (decline, seedling yellows, or stem pitting), to assess their aggressiveness or potential cross protectiveness (if any), and to monitor their movement into the host plants and the transmissibility by aphids.作者: 牌帶來(lái) 時(shí)間: 2025-3-26 14:06 作者: GRIN 時(shí)間: 2025-3-26 18:49
Bioinformatic Tools and Genome Analysis of , of novel viruses or viral strains as well as for the detection of known?viruses in a plant sample. Here we describe the entire process that needs to be considered for the genome analysis of . (CTV) by HTS, including the experimental design, sample preparation, nucleic acid purification, HTS library construction, and bioinformatic analysis.作者: 問(wèn)到了燒瓶 時(shí)間: 2025-3-26 21:09 作者: Modicum 時(shí)間: 2025-3-27 04:38 作者: 大都市 時(shí)間: 2025-3-27 07:09
Is Software Engineering , Engineering?ure can be performed in automated sequencers equipped with an appropriate capillary (CE-SSCP), which increases the potential of high-throughput analysis, precision, and the reproducibility of results. CE-SSCP can be also directly applied to the virus particles obtained by elution from ELISA plates or tissue-print membranes.作者: 平躺 時(shí)間: 2025-3-27 12:59 作者: 凹槽 時(shí)間: 2025-3-27 14:54
Takehisa Onisawa,Janusz Kacprzykniques used in previous studies yield very limited information about the strain composition of populations and the relative titer of their components. In this chapter we describe a protocol for the characterization of CTV populations, based on the use of the next-generation sequencing Illumina MiSeq platform of p33 gene amplicons.作者: Pert敏捷 時(shí)間: 2025-3-27 18:04 作者: 同來(lái)核對(duì) 時(shí)間: 2025-3-27 23:35
A Short Note on Reflections and Publications on , (CTV) Methodologies,hybridization probes, the genetic diversity among local CTV isolates was demonstrated. With the emergence of the PCR technology, we developed a CTV-dsRNA cloning method based on the ligation of known oligonucleotide molecules to dsRNA ends and the use of complementary oligonucleotides for cDNA synth作者: 衍生 時(shí)間: 2025-3-28 04:16
CTV Vectors and Interactions with the Virus and Host Plants,?h for each. CTV infection is readily determined by serology using a polyclonal antiserum or a monoclonal antiserum cocktail; whereas, molecular genotyping is conducted with reverse transcription polymerase chain (RT-PCR) or real time quantitavtive RT-PCR (RT-qPCR) with strain-specific primers and p作者: Expurgate 時(shí)間: 2025-3-28 06:15 作者: BUOY 時(shí)間: 2025-3-28 11:47 作者: 全等 時(shí)間: 2025-3-28 16:33 作者: 財(cái)主 時(shí)間: 2025-3-28 20:48
Amplification and Cloning of Large cDNA Fragments of the , Genome, CTV obtained from two RT-PCR amplified products. These 5′- and 3′-genomic halves, which were designed to be overlapped in 15?nt in their 3′- and 5′-ends, respectively, were used as templates for further overlapped PCR to amplify the entire ~20?kb CTV genome. The resultant full cDNA PCR product was 作者: Credence 時(shí)間: 2025-3-29 00:53 作者: GIST 時(shí)間: 2025-3-29 06:10 作者: 瑪瑙 時(shí)間: 2025-3-29 09:40
1064-3745 rologists, molecular biologists, and graduate students interested in performing qualitative and quantitative tests as well as recently-developed diagnostic methods in order to find solutions to improve the management of this disease..978-1-4939-9560-8978-1-4939-9558-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 引起 時(shí)間: 2025-3-29 13:59 作者: 羞辱 時(shí)間: 2025-3-29 18:34 作者: 惡意 時(shí)間: 2025-3-29 22:25
R. A. Adey,R. Harfield,C. A. Brebbiater, tissue-printing and squashing capture methods for direct sample preparation without extract preparation or nucleic acid extraction and purification were coupled with validated real-time RT-PCR detection protocols based on TaqMan chemistry for CTV detection.作者: Microgram 時(shí)間: 2025-3-30 02:39 作者: paleolithic 時(shí)間: 2025-3-30 04:24
Kai-Yuan Cai,Chuan-Yuan Wen,Ming-Lian Zhangully applied for rapid and sensitive detection of . (CTV) by using four oligo-primers, targeting a conserved coat protein gene (CPG) of an Indian CTV isolate. The result of assay is visible in naked eyes easily in the presence of SYBR Green I (100×) or on 1.5% agarose gel electrophoresis. CTV-RT-LAM作者: Ringworm 時(shí)間: 2025-3-30 08:31
Fuzzy Fault Tree Analysis : Case Studies CTV obtained from two RT-PCR amplified products. These 5′- and 3′-genomic halves, which were designed to be overlapped in 15?nt in their 3′- and 5′-ends, respectively, were used as templates for further overlapped PCR to amplify the entire ~20?kb CTV genome. The resultant full cDNA PCR product was 作者: 致命 時(shí)間: 2025-3-30 13:14 作者: CORD 時(shí)間: 2025-3-30 18:19
https://doi.org/10.1007/978-3-662-03041-7gth, untranslatable versions of the genes ., ., and . from the virus. Using it, we have generated full resistance against CTV in Mexican lime. Moreover, this strategy is applicable to all those citrus varieties amenable to mature transformation, including sweet oranges, sour oranges, mandarins, ., a作者: 有惡意 時(shí)間: 2025-3-30 22:25
Antonino F. Catara,Moshe Bar-Joseph,Grazia LicciarIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts作者: Intellectual 時(shí)間: 2025-3-31 03:57
Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/226799.jpg作者: orient 時(shí)間: 2025-3-31 06:16
Citrus Tristeza Virus978-1-4939-9558-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
