標(biāo)題: Titlebook: Circular RNAs; Christoph Dieterich,Marie-Laure Baudet Book 2024Latest edition The Editor(s) (if applicable) and The Author(s), under exclu [打印本頁(yè)] 作者: proptosis 時(shí)間: 2025-3-21 19:51
書(shū)目名稱(chēng)Circular RNAs影響因子(影響力)
作者: 安撫 時(shí)間: 2025-3-21 20:50 作者: 使熄滅 時(shí)間: 2025-3-22 02:53
Exploring Circular RNA Profile and Expression in Extracellular Vesiclesotential to modulate biological processes and are being investigated for their diagnostic and therapeutic applications. Circular RNAs (circRNAs), generated through back-splicing of exons, are enriched in EVs. Given their unique characteristics and diverse functions, EV-circRNAs are important players作者: congenial 時(shí)間: 2025-3-22 05:13
In Situ Hybridization of circRNAs in Cells and Tissues through BaseScope? Strategyecules. As for RNA, it is becoming clear how important is its intracellular localization for the control of proper cell differentiation and development and how its perturbation can be linked to several pathological states. This aspect is even more important if one thinks of highly polarized cells su作者: Cultivate 時(shí)間: 2025-3-22 09:12
Sanger Sequencing to Determine the Full-Length Sequence of Circular RNAsies using high throughput RNA-sequencing (RNA-seq) techniques and novel computational programs reported the abundant and ubiquitous expression of circRNAs originating by pre-mRNA backsplicing. CircRNAs are mostly involved in gene expression by regulating functions of interacting microRNAs (miRNAs) a作者: aptitude 時(shí)間: 2025-3-22 14:51 作者: aptitude 時(shí)間: 2025-3-22 17:37
Targeted Sequencing of Circular RNAs for Illumina-Based Counting and Nanopore Structure Determinatioe same linear transcripts that are canonically spliced to produce, for example, mature mRNAs. They exhibit tissue-specific expression pattern and are potentially involved in many diseases, among them cardiovascular diseases. However, despite the tremendous efforts to establish circRNA catalogues, mu作者: UNT 時(shí)間: 2025-3-22 22:40
Nanopore-Mediated Sequencing of Circular RNAcircRNAs are composed of more than one exon, which are spliced together in a linear fashion. This protocol describes methods to sequence full-length circRNA across the back-splicing junction, allowing unambiguous characterization of circRNA-specific exon-intron structures by long-read sequencing (LR作者: FLAG 時(shí)間: 2025-3-23 04:40
In Vivo Tissue-Specific Knockdown of circRNAs Using shRNAs in ar counterparts. This is particularly challenging as the back-splice junction is the only sequence not shared between the linear and circular version. In this chapter, we describe a method to study circRNA function in vivo targeting shRNAs against the desired back-splice junction to achieve knockdow作者: 山崩 時(shí)間: 2025-3-23 05:42
The Functional Circular RNA Screening via RfxCas13d/BSJ-gRNA Systemly. As circRNAs share overlapping sequences with their cognate linear RNAs, except for the back-splicing junction sites, it is difficult to distinguish circRNAs from cognate mRNAs in functional studies. In this chapter, we describe a programmable method for the large-scale functional circRNA screeni作者: 未完成 時(shí)間: 2025-3-23 09:59
Base-Editor-Mediated circRNA Knockout by Targeting Predominantly Back-Splice Sitesg sequences to their cognate linear RNAs from the same gene loci, leading to difficulties in distinguishing them from each other. A recent study has shown that some circRNAs can be specifically depleted by using base editing systems to target their predominantly back-splice sites for circularization作者: Minatory 時(shí)間: 2025-3-23 17:33 作者: 污點(diǎn) 時(shí)間: 2025-3-23 20:57 作者: covert 時(shí)間: 2025-3-24 00:05 作者: 教義 時(shí)間: 2025-3-24 05:02
Book 2024Latest editionation, circRNA detection, sequence validation, quantification , techniques related to? gain- and loss-of-function approaches, circular RNA synthesis, split ligation, engineering, nanoparticle packaging, RNA modifications on circular RNA biogenesis, RNA translation potential, and vaccines based on ci作者: 不適當(dāng) 時(shí)間: 2025-3-24 09:47
1064-3745 ation advice from the experts.This second edition details new and updated methods on circular RNA. Chapter guide readers through circular RNA purification, .in silico. characterization, circRNA detection, sequence validation, quantification , techniques related to? gain- and loss-of-function approac作者: 貪婪的人 時(shí)間: 2025-3-24 13:56 作者: candle 時(shí)間: 2025-3-24 18:14 作者: 鴕鳥(niǎo) 時(shí)間: 2025-3-24 21:58
Directed Circularization of a Short RNAelf-cleavage poorly. We designed an activator-polyamine complex to complete cleavage as a prerequisite for subsequent circularization. The developed protocol allows synthesizing circRNA without external enzymatic assistance and adds a controllable way of circularization to the existing methods.作者: COKE 時(shí)間: 2025-3-24 23:20 作者: 撤退 時(shí)間: 2025-3-25 04:42
https://doi.org/10.1007/978-3-030-43527-1 in disease pathology. This chapter describes a workflow for investigating the expression profile of EV-circRNAs, which includes EVs separation, library preparation, and bioinformatics analysis. This workflow can aid the investigation of EV-circRNAs and their potential role in disease pathology.作者: Jejune 時(shí)間: 2025-3-25 10:23
Cleveland S. Patterson,Nancy D. Urselng based on the RNA-guided, RNA-targeting CRISPR-Cas13 (RfxCas13d) system. This method can be applied both in vivo and in cell to explore highly expressed circRNAs that may influence cell growth, either under natural conditions or in response to environmental stimulation, without disturbing cognate linear mRNAs.作者: 遺留之物 時(shí)間: 2025-3-25 12:17 作者: glomeruli 時(shí)間: 2025-3-25 16:15 作者: Jogging 時(shí)間: 2025-3-25 21:52
Exploring Circular RNA Profile and Expression in Extracellular Vesicles in disease pathology. This chapter describes a workflow for investigating the expression profile of EV-circRNAs, which includes EVs separation, library preparation, and bioinformatics analysis. This workflow can aid the investigation of EV-circRNAs and their potential role in disease pathology.作者: UTTER 時(shí)間: 2025-3-26 01:47 作者: 潛移默化 時(shí)間: 2025-3-26 06:19
Base-Editor-Mediated circRNA Knockout by Targeting Predominantly Back-Splice Sites, suggesting an efficient approach for circRNA knockout (KO). Here, we describe the detailed protocol for applying base editors to disrupt back-splice sites of predominantly circularized exons for circRNA KO at the genomic DNA level.作者: Charitable 時(shí)間: 2025-3-26 10:37 作者: 上釉彩 時(shí)間: 2025-3-26 15:23
Book 2024Latest edition lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls...?..Authoritative and cutting-edge,?.Circular RNAs, Second Edition .aims to ensure successful results in the further study of this vital field..作者: indignant 時(shí)間: 2025-3-26 19:31 作者: LAP 時(shí)間: 2025-3-27 00:19
Nanopore-Mediated Sequencing of Circular RNAircRNA are sequenced without prior circRNA enrichment. Both methods were originally described in Karim et al. (Rahimi et al., Nat Commun 12: 4825, 2021) where they were applied to characterize the exon-intron structure of >10.000 circRNAs in mouse and human brains.作者: 噱頭 時(shí)間: 2025-3-27 02:48 作者: BET 時(shí)間: 2025-3-27 06:11
Regulating Technological Innovation to higher levels than their cognate linear mRNAs, but the vast majority are expressed at low levels. Hence, for most circRNAs, only a handful of sequencing reads, if any, that span the backsplicing junction are observed in standard RNA-seq libraries. It thus has become common to use the 3′–5′ exonu作者: 事先無(wú)準(zhǔn)備 時(shí)間: 2025-3-27 12:54
https://doi.org/10.1007/978-3-030-43527-1otic cells. Unlike canonical linear RNA molecules, circRNAs form a covalently closed continuous loop structure without a 5′ or 3′ end. They are generated by a process called back-splicing, in which a downstream splice donor site is joined to an upstream splice acceptor site. CircRNAs have been found作者: wreathe 時(shí)間: 2025-3-27 15:43
https://doi.org/10.1007/978-3-030-43527-1otential to modulate biological processes and are being investigated for their diagnostic and therapeutic applications. Circular RNAs (circRNAs), generated through back-splicing of exons, are enriched in EVs. Given their unique characteristics and diverse functions, EV-circRNAs are important players作者: Devastate 時(shí)間: 2025-3-27 19:48 作者: 能量守恒 時(shí)間: 2025-3-27 22:00
Problems in Covert Investigationsies using high throughput RNA-sequencing (RNA-seq) techniques and novel computational programs reported the abundant and ubiquitous expression of circRNAs originating by pre-mRNA backsplicing. CircRNAs are mostly involved in gene expression by regulating functions of interacting microRNAs (miRNAs) a作者: AGGER 時(shí)間: 2025-3-28 02:28 作者: 獨(dú)白 時(shí)間: 2025-3-28 06:53 作者: languor 時(shí)間: 2025-3-28 12:25 作者: 流浪 時(shí)間: 2025-3-28 17:21
https://doi.org/10.1007/978-1-349-08714-3ar counterparts. This is particularly challenging as the back-splice junction is the only sequence not shared between the linear and circular version. In this chapter, we describe a method to study circRNA function in vivo targeting shRNAs against the desired back-splice junction to achieve knockdow作者: Abnormal 時(shí)間: 2025-3-28 20:56 作者: Recessive 時(shí)間: 2025-3-29 01:03
Joaquín Bernáldez,Rocío Juliana Herrerag sequences to their cognate linear RNAs from the same gene loci, leading to difficulties in distinguishing them from each other. A recent study has shown that some circRNAs can be specifically depleted by using base editing systems to target their predominantly back-splice sites for circularization作者: 十字架 時(shí)間: 2025-3-29 05:18 作者: 使聲音降低 時(shí)間: 2025-3-29 08:49
James D. Bradbury,Courtney Cox Smithrpart and can be designed for efficient expression in different cell and tissue types. In this chapter, we developed different backsplicing circRNA cassettes that can enable efficient gene expression in various cell and tissue types. Furthermore, we packaged cassettes encoding circRNAs into adeno-as作者: Mangle 時(shí)間: 2025-3-29 15:18 作者: Brocas-Area 時(shí)間: 2025-3-29 19:06
https://doi.org/10.1007/978-1-0716-3678-7splice-aware pseudo-alignment scheme; paired-end RNA-sequencing; ddPCR; CLIP-seq; Bioinformatics作者: Fluctuate 時(shí)間: 2025-3-29 20:42 作者: 不朽中國(guó) 時(shí)間: 2025-3-30 00:39
Christoph Dieterich,Marie-Laure BaudetIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: 遷移 時(shí)間: 2025-3-30 07:52 作者: 柳樹(shù)；枯黃 時(shí)間: 2025-3-30 09:22
Circular RNAs978-1-0716-3678-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: originality 時(shí)間: 2025-3-30 13:47
https://doi.org/10.1007/978-1-349-08714-3ar counterparts. This is particularly challenging as the back-splice junction is the only sequence not shared between the linear and circular version. In this chapter, we describe a method to study circRNA function in vivo targeting shRNAs against the desired back-splice junction to achieve knockdown with tissue-specific resolution in flies.作者: 減至最低 時(shí)間: 2025-3-30 20:17 作者: 變白 時(shí)間: 2025-3-30 23:33
https://doi.org/10.1007/978-3-030-43527-1ilico from RNA sequencing data using the . circRNA analytics software suite. The protocol starts from raw sequencing data with circRNA detection via back-splice events and includes statistical testing of circRNAs as well as primer design for follow-up wet lab experiments.作者: 上下連貫 時(shí)間: 2025-3-31 04:56 作者: 召集 時(shí)間: 2025-3-31 05:21 作者: capillaries 時(shí)間: 2025-3-31 12:20
https://doi.org/10.1007/978-981-33-6381-6l PCR (ddPCR) is rapidly taking over for the more popular qPCR. In this chapter, we describe the detailed procedure based on droplets partitioning to quantify both linear and circRNA abundancy and demonstrate the circularity of the transcript under study with high precision, in a single experiment.
