標(biāo)題: Titlebook: Circadian Clocks; Methods and Protocol Steven A. Brown Book 2021 Springer Science+Business Media, LLC, part of Springer Nature 2021 Neurosp [打印本頁(yè)] 作者: 法官所用 時(shí)間: 2025-3-21 18:56
書目名稱Circadian Clocks影響因子(影響力)
書目名稱Circadian Clocks影響因子(影響力)學(xué)科排名
書目名稱Circadian Clocks網(wǎng)絡(luò)公開度
書目名稱Circadian Clocks網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Circadian Clocks被引頻次
書目名稱Circadian Clocks被引頻次學(xué)科排名
書目名稱Circadian Clocks年度引用
書目名稱Circadian Clocks年度引用學(xué)科排名
書目名稱Circadian Clocks讀者反饋
書目名稱Circadian Clocks讀者反饋學(xué)科排名
作者: Somber 時(shí)間: 2025-3-21 22:38
Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/226566.jpg作者: 動(dòng)作謎 時(shí)間: 2025-3-22 03:34 作者: Lipohypertrophy 時(shí)間: 2025-3-22 07:36 作者: 陰謀 時(shí)間: 2025-3-22 10:55
Real-Time In Vitro Fluorescence Anisotropy of the Cyanobacterial Circadian Clock randomization process is slower for larger molecules, fluorescence anisotropy is effective for measuring the kinetics of protein-binding events. Here, we describe how to use the technique to carry out real-time observations in vitro of the cyanobacterial circadian clock.作者: 神圣將軍 時(shí)間: 2025-3-22 15:38 作者: 神圣將軍 時(shí)間: 2025-3-22 19:15
https://doi.org/10.1007/978-1-349-05789-4 randomization process is slower for larger molecules, fluorescence anisotropy is effective for measuring the kinetics of protein-binding events. Here, we describe how to use the technique to carry out real-time observations in vitro of the cyanobacterial circadian clock.作者: ESO 時(shí)間: 2025-3-22 23:00
Regional Organization and Regional Economicsainst pathogens) and the adaptive immunity (a more specific set of responses, which lead to immune memory). Here, to illustrate principles to be taken into account when working on circadian rhythms in immunology experiments, two protocols will be presented. The first one aims to analyze immune param作者: Blood-Clot 時(shí)間: 2025-3-23 01:44 作者: doxazosin 時(shí)間: 2025-3-23 07:34
Luk Van Langenhove,Stephen Kingahcohorts imposes itself. Here, we describe potentials and limitations of using questionnaires, specifically the Munich ChronoType Questionnaire (MCTQ), to characterize such circadian phenotypes. We also discuss scenarios when alternative methods might be more appropriate.作者: 飛來飛去真休 時(shí)間: 2025-3-23 10:59
Tullo Vigevani,Juliano A. S. AragusukuMathematical modeling supports understanding available heterogeneous kinetic data, highlights basic mechanisms, and can guide experimental research. Here, we introduce the basic steps from a biological question to simple models providing insight into gene-regulatory mechanisms. We illustrate the gen作者: DAFT 時(shí)間: 2025-3-23 14:25 作者: 背帶 時(shí)間: 2025-3-23 19:18 作者: 指耕作 時(shí)間: 2025-3-24 01:56
https://doi.org/10.1057/9781137490353at oscillating enhancer activity is a major driving force of rhythmic gene transcription. Functional circadian enhancers can be identified in an unbiased manner by correlation with the rhythms of nearby gene transcription..Global run-on sequencing (GRO-seq) measures nascent transcription of both pre作者: inflame 時(shí)間: 2025-3-24 05:52
Palgrave Studies in International Relationsanels of small biomolecules fluctuating in a circadian fashion, thus enabling a more precise determination of inner time and understanding of how circadian clock operates at the molecular level. Emerging analytical techniques allowing for the determination of exhaled metabolites in breath show promi作者: photophobia 時(shí)間: 2025-3-24 07:47 作者: 單純 時(shí)間: 2025-3-24 10:54
An Overview of the State of Media in Africa, lipid metabolic changes into this analysis allows to access yet another important aspect of lipid regulation. The resulting methodology, circadian lipidomics, has thus emerged as a novel tool to address the enormous complexity, which is present among cellular lipids. Here, we describe how mass spec作者: bronchodilator 時(shí)間: 2025-3-24 15:39
Historical Landscape of Nigerian Newspapers,nslational modifications (PTMs) in relatively short times. Therefore, in the last few years, this technology has proven successful in the circadian field to characterize temporal oscillations of the proteome and more recently PTMs in cellular systems and in tissues. In this chapter, we describe a ro作者: ADAGE 時(shí)間: 2025-3-24 21:59 作者: itinerary 時(shí)間: 2025-3-25 03:14
https://doi.org/10.1007/978-981-10-0242-7 molecular clock and neuronal communication within individual neurons and throughout the entire circadian circuitry. Here we describe how to establish brain explants and dissociated neuron culture from . larvae, guidelines for time-lapse fluorescence microscopy, and the method of image analysis. Thi作者: GLIDE 時(shí)間: 2025-3-25 06:07 作者: insightful 時(shí)間: 2025-3-25 10:21
https://doi.org/10.1007/978-3-030-90398-5The forced swim and tail suspension tests are commonly used to determine the effects of circadian-related pharmacological, genetic, and environmental manipulations on depression-like behavior in rodents. Both tests involve scoring immobility of rodents in an inescapable condition. Here we describe how to set up and carry out these tests.作者: Meditative 時(shí)間: 2025-3-25 12:45
Inductively Coupled Plasma Mass Spectrometry for Elemental Analysis in Circadian BiologyInductively coupled plasma mass spectrometry (ICP-MS) is a sensitive instrumental analysis technique used for multielemental and isotopic determination. Here we provide a sample preparation and circadian ICP-MS analysis protocol for use with mammalian tissues and cells, using mouse fibroblasts as a case study.作者: INERT 時(shí)間: 2025-3-25 19:37
Measuring the Effects of Circadian Rhythm-Related Manipulations on Depression-Like Behavior in RodenThe forced swim and tail suspension tests are commonly used to determine the effects of circadian-related pharmacological, genetic, and environmental manipulations on depression-like behavior in rodents. Both tests involve scoring immobility of rodents in an inescapable condition. Here we describe how to set up and carry out these tests.作者: 榮幸 時(shí)間: 2025-3-25 23:28
Circadian Clocks978-1-0716-0381-9Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: AMEND 時(shí)間: 2025-3-26 03:17
https://doi.org/10.1007/978-1-349-05789-4 randomization process is slower for larger molecules, fluorescence anisotropy is effective for measuring the kinetics of protein-binding events. Here, we describe how to use the technique to carry out real-time observations in vitro of the cyanobacterial circadian clock.作者: inquisitive 時(shí)間: 2025-3-26 05:17 作者: 我不明白 時(shí)間: 2025-3-26 11:52
Real-Time In Vitro Fluorescence Anisotropy of the Cyanobacterial Circadian Clock randomization process is slower for larger molecules, fluorescence anisotropy is effective for measuring the kinetics of protein-binding events. Here, we describe how to use the technique to carry out real-time observations in vitro of the cyanobacterial circadian clock.作者: ALOFT 時(shí)間: 2025-3-26 16:26
The Assessment of Circadian Rhythms Within the Immune Systemainst pathogens) and the adaptive immunity (a more specific set of responses, which lead to immune memory). Here, to illustrate principles to be taken into account when working on circadian rhythms in immunology experiments, two protocols will be presented. The first one aims to analyze immune param作者: dainty 時(shí)間: 2025-3-26 19:42
Measuring Circadian Rhythms in Human Cellssources that bridge the gap between animal models and human subjects in basic as well as clinical research. The cells also retain molecular circadian clocks that reflect subject-specific differences in circadian physiology, and the cellular rhythms can be measured easily in large scale. This is a se作者: cravat 時(shí)間: 2025-3-26 21:31 作者: 思考而得 時(shí)間: 2025-3-27 04:26
Simple Kinetic Models in Molecular ChronobiologyMathematical modeling supports understanding available heterogeneous kinetic data, highlights basic mechanisms, and can guide experimental research. Here, we introduce the basic steps from a biological question to simple models providing insight into gene-regulatory mechanisms. We illustrate the gen作者: forbid 時(shí)間: 2025-3-27 08:27 作者: BATE 時(shí)間: 2025-3-27 13:24
Chromatin Immunoprecipitation (ChIP) from Mouse Liver Nucleihe circadian oscillator. Chromatin immunoprecipitation (ChIP) enables the detection and temporal analysis of such binding events in vivo. Here, we describe the individual steps from the generation of formaldehyde-cross-linked chromatin from mouse liver nuclei, fragmentation thereof, immunoprecipitat作者: collagenase 時(shí)間: 2025-3-27 15:34 作者: 不規(guī)則的跳動(dòng) 時(shí)間: 2025-3-27 19:01
Circadian Metabolomics from Breathanels of small biomolecules fluctuating in a circadian fashion, thus enabling a more precise determination of inner time and understanding of how circadian clock operates at the molecular level. Emerging analytical techniques allowing for the determination of exhaled metabolites in breath show promi作者: 忘川河 時(shí)間: 2025-3-27 22:24 作者: 瑣碎 時(shí)間: 2025-3-28 04:55 作者: Inflammation 時(shí)間: 2025-3-28 09:30 作者: 復(fù)習(xí) 時(shí)間: 2025-3-28 10:36 作者: 鞠躬 時(shí)間: 2025-3-28 17:56 作者: resuscitation 時(shí)間: 2025-3-28 22:26
Book 2021stems. The chapters are organized into three parts and cover topics such as measuring and modeling physiological and behavioral rhythms; genome-wide analyses in circadian biology, and imaging and manipulating brain clocks. Written in the highly successful .Methods in Molecular Biology. series format作者: 投射 時(shí)間: 2025-3-29 01:30
Measuring Circadian Rhythms in Human Cellsries of protocols that describes the procedure to measure circadian rhythms in these cells, starting from deriving fibroblasts from skin punch biopsy, to generation of stable cells expressing a circadian reporter, and finally measurement of cellular rhythms in large scale.作者: Offbeat 時(shí)間: 2025-3-29 03:25 作者: MIRE 時(shí)間: 2025-3-29 07:40 作者: 竊喜 時(shí)間: 2025-3-29 14:45 作者: 寡頭政治 時(shí)間: 2025-3-29 17:54
Fluorescence Live Imaging of , Circadian Pacemaker Neuronss approach enables the long-term monitoring of fluorescence signals of circadian reporters at single-cell resolution and can be also applicable to analyze real-time expression of other fluorescent probes in . neurons.作者: 詞匯記憶方法 時(shí)間: 2025-3-29 20:32
Governance and Limited Statehoodries of protocols that describes the procedure to measure circadian rhythms in these cells, starting from deriving fibroblasts from skin punch biopsy, to generation of stable cells expressing a circadian reporter, and finally measurement of cellular rhythms in large scale.作者: 對(duì)待 時(shí)間: 2025-3-30 00:14
Andrea C. Bianculli,Andrea Ribeiro Hoffmannion, reversal of cross-links, fragment cleanup, and detection of binding sites by real-time PCR. Depending on the quality of the employed antibody, a clear enrichment signal over the background is expected with a resolution of about 500–800 base pairs around the selected primer–probe pair.作者: 樸素 時(shí)間: 2025-3-30 08:05 作者: 誘使 時(shí)間: 2025-3-30 09:41
Historical Landscape of Nigerian Newspapers,bust and simple protocol, based on the EasyPhos workflow, to enable preparation of large number of proteomes and phosphoproteomes from mouse tissues for MS-based quantitative analysis. We additionally discuss computational methods to analyze proteome and phosphoproteome time series to determine circadian oscillations.作者: corn732 時(shí)間: 2025-3-30 13:36 作者: 煤渣 時(shí)間: 2025-3-30 20:04 作者: OCTO 時(shí)間: 2025-3-30 21:02
Regional Organization and Regional Economicsafter immunization of mice with antigen presenting cells loaded with a peptide antigen. These two protocols are optimized for circadian experiments, and outcome measures are mainly based on flow cytometry, which allows analysis of different parameters in the same cells.作者: opprobrious 時(shí)間: 2025-3-31 03:11
https://doi.org/10.1057/9781137490353. Moreover, using gene-eRNA correlation, we detail the steps necessary to identify functional enhancers and transcription factors (TFs) that control circadian gene expression in vivo. While we use mouse liver as an example, this protocol is applicable for multiple tissues.作者: 的’ 時(shí)間: 2025-3-31 08:36
The Assessment of Circadian Rhythms Within the Immune Systemafter immunization of mice with antigen presenting cells loaded with a peptide antigen. These two protocols are optimized for circadian experiments, and outcome measures are mainly based on flow cytometry, which allows analysis of different parameters in the same cells.
