標題: Titlebook: Cilia; Methods and Protocol Peter Satir,S?ren Tvorup Christensen Book 2016 Springer Science+Business Media New York 2016 intraflagellar tra [打印本頁] 作者: 遠見 時間: 2025-3-21 17:33
書目名稱Cilia影響因子(影響力)
書目名稱Cilia影響因子(影響力)學(xué)科排名
書目名稱Cilia網(wǎng)絡(luò)公開度
書目名稱Cilia網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Cilia被引頻次
書目名稱Cilia被引頻次學(xué)科排名
書目名稱Cilia年度引用
書目名稱Cilia年度引用學(xué)科排名
書目名稱Cilia讀者反饋
書目名稱Cilia讀者反饋學(xué)科排名
作者: 占卜者 時間: 2025-3-21 22:42
Targeting of ASH Domain-Containing Proteins to the Centrosome,he identification of a vast number of candidate ciliary or centrosomal proteins, whose subcellular localization needs to be investigated and validated. Here, we describe a simple and inexpensive method for analyzing the subcellular localization of candidate cilium- or centrosome-associated proteins,作者: 類似思想 時間: 2025-3-22 03:22 作者: Mnemonics 時間: 2025-3-22 07:23
Methods to Study Interactions Between Ciliogenesis and Autophagy,autophagy activity can be monitored by a variety of biochemical and functional assays that should be used in combination. Recently, it has been described that signaling from the primary cilium modulates autophagy. This novel and reciprocal interaction will impact diverse aspects of the cell biology 作者: CLASH 時間: 2025-3-22 12:28
Recombinant Reconstitution and Purification of the IFT-B Core Complex from ,,ary base and tip. IFT is mediated by the multi-subunit IFT complex, which simultaneously binds cargo proteins and the ciliary motors. So far 22 subunits of the IFT complex have been identified, but insights into the biochemical architecture and especially the three-dimensional structure of this mach作者: preeclampsia 時間: 2025-3-22 16:51 作者: preeclampsia 時間: 2025-3-22 19:13 作者: 啪心兒跳動 時間: 2025-3-22 22:25 作者: 大溝 時間: 2025-3-23 04:08 作者: 招致 時間: 2025-3-23 09:27
Methods for Studying Ciliary-Mediated Chemoresponse in ,,emoresponse..Unicellular organisms like the ciliated protozoan . sense and respond to chemicals in their environment (Van Houten, Ann Rev Physiol 54:639–663, 1992; Van Houten, Trends Neurosci 17:62–71, 1994). A thousand or more cilia that cover . cells serve as antennae for chemical signals, similar作者: 跑過 時間: 2025-3-23 12:36
STED and STORM Superresolution Imaging of Primary Cilia,tudies of ciliary proteins. Superresolution microscopy can reach up to a 20 nm resolution, significantly improving the ability to map molecules in primary cilia. Here we describe detailed experimental procedure of STED microscopy imaging and dSTORM imaging, two of the most powerful superresolution i作者: grandiose 時間: 2025-3-23 15:50 作者: DEFER 時間: 2025-3-23 18:05
Methods for Visualization of Neuronal Cilia,es in neuronal specification, maintenance, and function. In mammals, the most common cilia marker, acetylated α-tubulin, is extremely difficult to detect in neuronal cilia. Here, we describe methods to detect neuronal cilia in culture, in fixed sections, and in vivo, taking advantage of transgenic m作者: FLING 時間: 2025-3-24 01:16 作者: 可耕種 時間: 2025-3-24 04:37
Analysis of Axonemal Assembly During Ciliary Regeneration in ,,. to analyze the sequence in which ciliary proteins assemble. In addition, the methods described allow analysis of the mechanisms involved in regulation of ciliary length, the proteins required for ciliary assembly, and the temporal expression of genes encoding ciliary proteins. Ultimately, these me作者: Evolve 時間: 2025-3-24 07:50
, as a Model System for the Analysis of Ciliary Assembly and Motility,lia are exposed to the extracellular environment. These beat in a synchronized manner against secreted mucus thereby propelling the animal forward. Similar to the nematode ., the planarian . is easy to maintain in the laboratory and is highly amenable to simple RNAi approaches through feeding with d作者: 神秘 時間: 2025-3-24 11:10 作者: 有權(quán) 時間: 2025-3-24 16:02
Dimensionen informellen Regierenses in neuronal specification, maintenance, and function. In mammals, the most common cilia marker, acetylated α-tubulin, is extremely difficult to detect in neuronal cilia. Here, we describe methods to detect neuronal cilia in culture, in fixed sections, and in vivo, taking advantage of transgenic mice carrying fluorescently tagged cilia proteins.作者: Harrowing 時間: 2025-3-24 20:12 作者: Glossy 時間: 2025-3-24 23:58 作者: 切碎 時間: 2025-3-25 05:59
https://doi.org/10.1007/978-3-531-90375-0he identification of a vast number of candidate ciliary or centrosomal proteins, whose subcellular localization needs to be investigated and validated. Here, we describe a simple and inexpensive method for analyzing the subcellular localization of candidate cilium- or centrosome-associated proteins,作者: Occlusion 時間: 2025-3-25 09:42 作者: 刺耳的聲音 時間: 2025-3-25 13:51
Reformpotenzial von Regierungssystemen,autophagy activity can be monitored by a variety of biochemical and functional assays that should be used in combination. Recently, it has been described that signaling from the primary cilium modulates autophagy. This novel and reciprocal interaction will impact diverse aspects of the cell biology 作者: LVAD360 時間: 2025-3-25 18:08 作者: fatuity 時間: 2025-3-25 23:51
https://doi.org/10.1007/978-3-531-90375-0anelle. Proteins move into and inside cilia by diffusion and by motor-based intraflagellar transport (IFT). Many aspects of ciliary protein transport such as the distribution of unloading sites and the frequency of transport can be analyzed using direct in vivo imaging of fluorescently tagged protei作者: Clumsy 時間: 2025-3-26 01:50
Postkommunistische Regierungssystemvarianz,ime and effective vectorial transport to the cilium is essential for the maintenance of the photoreceptor cell function. Defects in molecules of transport modules lead to severe retinal ciliopathies. We have recently established a fluorescence recovery after photobleaching (FRAP)-based method to mon作者: tolerance 時間: 2025-3-26 05:04
Postkommunistische Regierungssystemvarianz, of a single anterograde IFT motor, heterotrimeric kinesin-II, is required to generate two identical motile flagella. The function of this canonical anterograde IFT motor is conserved among all eukaryotes, yet multicellular organisms can generate cilia of diverse structures and functions, ranging fr作者: induct 時間: 2025-3-26 10:28
https://doi.org/10.1007/978-3-663-11278-5ght organizer (LRO). Perception of cilia-directed flow triggers a calcium signaling cascade which originates within the cilium itself and then is relayed into the surrounding mesendodermal tissue, thereby directing organ situs via the nodal pathway. Two types of cilia, motile and immotile, function 作者: 禁止,切斷 時間: 2025-3-26 14:14
https://doi.org/10.1007/978-3-663-11278-5emoresponse..Unicellular organisms like the ciliated protozoan . sense and respond to chemicals in their environment (Van Houten, Ann Rev Physiol 54:639–663, 1992; Van Houten, Trends Neurosci 17:62–71, 1994). A thousand or more cilia that cover . cells serve as antennae for chemical signals, similar作者: 反復(fù)拉緊 時間: 2025-3-26 19:07 作者: 冒煙 時間: 2025-3-26 21:19 作者: 奴才 時間: 2025-3-27 01:52 作者: 刪減 時間: 2025-3-27 06:09 作者: 似少年 時間: 2025-3-27 13:28
https://doi.org/10.1007/978-981-99-3272-6. to analyze the sequence in which ciliary proteins assemble. In addition, the methods described allow analysis of the mechanisms involved in regulation of ciliary length, the proteins required for ciliary assembly, and the temporal expression of genes encoding ciliary proteins. Ultimately, these me作者: 翻布尋找 時間: 2025-3-27 17:09 作者: semiskilled 時間: 2025-3-27 18:22 作者: impale 時間: 2025-3-27 23:29
Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/226526.jpg作者: Allowance 時間: 2025-3-28 04:53
https://doi.org/10.1007/978-1-4939-3789-9intraflagellar transport (IFT); cell organelle; motile cilia; sensory cilia; flagellate algae; ciliates; p作者: occurrence 時間: 2025-3-28 10:16
978-1-4939-8139-7Springer Science+Business Media New York 2016作者: 決定性 時間: 2025-3-28 11:55
https://doi.org/10.1007/978-3-531-90375-0ative immunofluorescence microscopy (IFM) analysis in cultured human telomerase-immortalized retinal pigmented epithelial (hTERT-RPE1) cells, we could confirm that TRAPPC13 and NPHP4 are highly enriched at the base of primary cilia, whereas DLEC1 seems to associate specifically with motile cilia. Re作者: 退出可食用 時間: 2025-3-28 15:59
Postkommunistische Regierungssystemvarianz,of motor molecules..Here, we describe in detail our method for measuring the motility of proteins in cilia or dendrites of . male-specific CEM ciliated sensory neurons using time-lapse microscopy and kymography of green fluorescent protein (GFP)-tagged motors, receptors, and cargos. We describe, as 作者: MUTE 時間: 2025-3-28 20:37 作者: 使成波狀 時間: 2025-3-28 23:18
https://doi.org/10.1007/978-981-99-3272-6bined, these feature make planaria a useful model system in which to examine ciliary assembly and motility, especially in the context of a ciliated epithelium where many organelles beat in a hydrodynamically coupled synchronized manner. In addition, as planaria secrete mucus against which the cilia 作者: 啜泣 時間: 2025-3-29 05:43 作者: Afflict 時間: 2025-3-29 09:45 作者: muscle-fibers 時間: 2025-3-29 12:08
Targeting of ASH Domain-Containing Proteins to the Centrosome,ative immunofluorescence microscopy (IFM) analysis in cultured human telomerase-immortalized retinal pigmented epithelial (hTERT-RPE1) cells, we could confirm that TRAPPC13 and NPHP4 are highly enriched at the base of primary cilia, whereas DLEC1 seems to associate specifically with motile cilia. Re作者: infantile 時間: 2025-3-29 15:43
Kymographic Analysis of Transport in an Individual Neuronal Sensory Cilium in ,,of motor molecules..Here, we describe in detail our method for measuring the motility of proteins in cilia or dendrites of . male-specific CEM ciliated sensory neurons using time-lapse microscopy and kymography of green fluorescent protein (GFP)-tagged motors, receptors, and cargos. We describe, as 作者: 沙草紙 時間: 2025-3-29 22:02
Methods for Studying Ciliary-Mediated Chemoresponse in ,,(1) assaying populations of cells for their behavioral responses to chemicals (attraction and repulsion), (2) characterization of the chemoreceptors and associated channels of the cilia using proteomics and binding assays, and (3) electrophysiological analysis of individual cells’ responses to chemi作者: HARP 時間: 2025-3-30 03:57
, as a Model System for the Analysis of Ciliary Assembly and Motility,bined, these feature make planaria a useful model system in which to examine ciliary assembly and motility, especially in the context of a ciliated epithelium where many organelles beat in a hydrodynamically coupled synchronized manner. In addition, as planaria secrete mucus against which the cilia 作者: hegemony 時間: 2025-3-30 07:50 作者: 不如樂死去 時間: 2025-3-30 10:52
CLEM Methods for Studying Primary Cilia,zes image manipulation in correlating the fluorescent and SEM images. Alternative methods prepare samples for fluorescence imaging followed by processing for SEM then observation in the SEM. This method is ideal for optimal fluorescence imaging, particularly live cell imaging.作者: 流出 時間: 2025-3-30 16:02 作者: 妨礙 時間: 2025-3-30 17:09 作者: aesthetician 時間: 2025-3-30 20:56 作者: Ostrich 時間: 2025-3-31 03:05 作者: 有危險 時間: 2025-3-31 07:00 作者: 招惹 時間: 2025-3-31 10:01 作者: 尾巴 時間: 2025-3-31 15:20
Postkommunistische Regierungssystemvarianz,photoreceptor cells of native vibratome sections through the retina by high laser intensity. In the laser scanning microscope, the recovery of the fluorescent signal is monitored over time and the kinetics of movements of molecules can be quantitatively ascertained.作者: 矛盾心理 時間: 2025-3-31 19:19 作者: Rinne-Test 時間: 2025-3-31 23:42
Methods for Studying Ciliary Import Mechanisms,s into ciliated cells. We also describe a fluorescence recovery after photobleaching (FRAP) assay to measure the entry of ciliary proteins into the ciliary compartment. These assays can be used to determine the molecular mechanisms that regulate the formation and function of cilia in mammalian cells.作者: 喚起 時間: 2025-4-1 04:35
Recombinant Reconstitution and Purification of the IFT-B Core Complex from ,,ocol for the purification and reconstitution of a complex containing nine . IFT proteins, commonly known as the IFT-B core complex. In our hands, this protocol routinely yields several milligrams of pure complex suitable for structural analysis by X-ray crystallography and single-particle cryo-electron microscopy.作者: 惡心 時間: 2025-4-1 08:55 作者: 殺菌劑 時間: 2025-4-1 11:01 作者: COM 時間: 2025-4-1 17:15
1064-3745 ation advice from the experts.This volume is the result of an explosion ofmolecular-based research on Cilia, which began with the discovery of theuniversality of intraflagellar transport (IFT) and ciliary genomics/proteomics.The chapters in this book cover topics such as: high resolution imaging and作者: NIP 時間: 2025-4-1 22:13 作者: analogous 時間: 2025-4-1 23:48
Reformpotenzial von Regierungssystemen,bed that signaling from the primary cilium modulates autophagy. This novel and reciprocal interaction will impact diverse aspects of the cell biology in healthy and pathophysiological conditions. Here, we describe methods to monitor autophagy activity in cilia mutants, as well as the use of autophagy mutants to monitor ciliogenesis.
