標(biāo)題: Titlebook: Chromatin; Methods and Protocol Julia Horsfield,Judith Marsman Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive l [打印本頁(yè)] 作者: papyrus 時(shí)間: 2025-3-21 20:07
書目名稱Chromatin影響因子(影響力)
書目名稱Chromatin影響因子(影響力)學(xué)科排名
書目名稱Chromatin網(wǎng)絡(luò)公開度
書目名稱Chromatin網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Chromatin被引頻次
書目名稱Chromatin被引頻次學(xué)科排名
書目名稱Chromatin年度引用
書目名稱Chromatin年度引用學(xué)科排名
書目名稱Chromatin讀者反饋
書目名稱Chromatin讀者反饋學(xué)科排名
作者: 馬籠頭 時(shí)間: 2025-3-21 21:40 作者: Boycott 時(shí)間: 2025-3-22 03:33 作者: Discrete 時(shí)間: 2025-3-22 05:05 作者: opportune 時(shí)間: 2025-3-22 11:47 作者: 灌輸 時(shí)間: 2025-3-22 16:03 作者: 灌輸 時(shí)間: 2025-3-22 18:07 作者: ECG769 時(shí)間: 2025-3-22 23:38
High-Resolution ATAC-Seq Analysis of Frozen Clinical Tissuesintra-tissue heterogeneity. Here we describe our method for nuclei isolation from frozen specimens with wide applicability across tissue types, producing nuclei suitable for a number of molecular profiling methods including ATAC-seq in bulk and at a single-cell resolution.作者: 脖子 時(shí)間: 2025-3-23 03:46 作者: 積習(xí)已深 時(shí)間: 2025-3-23 07:55
Reconstituting the State in Africant of CRISPR-based technologies has revolutionized our potential for site-specific epigenomic editing. Here, we provide a detailed protocol for the design, construction, and utilization of a transient, CRISPR-based DNA methylation-editing system in mammalian cells.作者: 模仿 時(shí)間: 2025-3-23 13:35
Editing of DNA Methylation Patterns Using CRISPR-Based Toolsnt of CRISPR-based technologies has revolutionized our potential for site-specific epigenomic editing. Here, we provide a detailed protocol for the design, construction, and utilization of a transient, CRISPR-based DNA methylation-editing system in mammalian cells.作者: AVERT 時(shí)間: 2025-3-23 15:32 作者: 并入 時(shí)間: 2025-3-23 20:47
‘Education’ and the Pruning of the Mindsolution mass spectrometry, TINC enables the identification and characterization of protein complexes formed at any RE of interest. Using the . promoter in mouse embryonic stem cells as proof of concept, this chapter describes in detail the novel TINC methodology as well as subsequent mass spectrometric considerations.作者: 壓艙物 時(shí)間: 2025-3-24 00:48
‘Education’ and the Pruning of the Mindt form large chromatin domains or that are part of distinct nuclear structures such as the nuclear lamina. This chapter describes the pA-DamID procedure from cell harvesting to the preparation of microscopy slides and high-throughput sequencing libraries.作者: 一大塊 時(shí)間: 2025-3-24 06:22
A Protocol for Studying Transcription Factor Dynamics Using Fast Single-Particle Tracking and Spot-Olyze these trajectories using the kinetic modeling package Spot-On. We discuss how to use Spot-On to fit histograms of displacements and extract useful information such as the fraction of TFs that are bound and freely diffusing, and their associated diffusion coefficients.作者: 帶子 時(shí)間: 2025-3-24 08:20 作者: 幻影 時(shí)間: 2025-3-24 14:30
Genome-Wide Mapping and Microscopy Visualization of Protein–DNA Interactions by pA-DamIDt form large chromatin domains or that are part of distinct nuclear structures such as the nuclear lamina. This chapter describes the pA-DamID procedure from cell harvesting to the preparation of microscopy slides and high-throughput sequencing libraries.作者: 征兵 時(shí)間: 2025-3-24 17:14
Book 2022d proteins affect the transcriptional output of the genome..Chapter Genome-wide mapping and microscopy visualization of protein-DNA interactions by pA-DamID?[Chapter 12] is available open access undera Creative Commons Attribution 4.0 International License via link.springer.com..作者: violate 時(shí)間: 2025-3-24 21:24 作者: 凝視 時(shí)間: 2025-3-24 23:28
Generating Sequencing-Based DNA Methylation Maps from Low DNA Input Samplesoduced to focus on CpG-rich regions that are likely to be of most interest for epigenetic regulation, such as gene promoters and enhancer sequence elements (Meissner et al., Nature 454:766–770, 2008). This “reduced representation” lowers the cost of sequencing and also gives increased depth of cover作者: agnostic 時(shí)間: 2025-3-25 06:58 作者: Tdd526 時(shí)間: 2025-3-25 09:15 作者: 點(diǎn)燃 時(shí)間: 2025-3-25 13:56 作者: unstable-angina 時(shí)間: 2025-3-25 16:46
Nanopore Sequencing and Data Analysis for Base-Resolution Genome-Wide 5-Methylcytosine Profilinge nature of sodium bisulfite results in DNA fragmentation and subsequent biases in sequencing data. Such issues have led to the development of bisulfite-free methods for 5mC detection. Nanopore sequencing is a long read nondestructive approach that directly analyzes DNA and RNA fragments in real tim作者: 砍伐 時(shí)間: 2025-3-25 22:50
Chromatin Immunoprecipitation Sequencing (ChIP-seq) Protocol for Small Amounts of Frozen Biobanked Cng histone modifications and DNA–protein interactions. It provides valuable insights to better understand disease mechanisms. Here we present an optimized ChIP-seq protocol suitable for human cardiac tissues, especially the frozen biobanked small biopsy samples.作者: modest 時(shí)間: 2025-3-26 00:23
A Robust Protocol for Investigating the Cohesin Complex by ChIP-SequencingCTCF collaborate to form chromatin loops and to gain insight in the intricate regulation of cohesin. Here we describe a detailed ChIP protocol that has been successfully used for different cohesin subunits and cohesin regulators in various cell lines.作者: antipsychotic 時(shí)間: 2025-3-26 07:43
Epi-Decoder: Decoding the Local Proteome of a Genomic Locus by Massive Parallel Chromatin Immunoprecnd their complexes have been identified before, but how each genomic locus interacts with its surrounding proteins in the nucleus over time and in changing conditions remains poorly described. Measuring protein–DNA interactions at a specific locus in the genome is challenging and current techniques 作者: 詳細(xì)目錄 時(shí)間: 2025-3-26 09:38
A Protocol for Studying Transcription Factor Dynamics Using Fast Single-Particle Tracking and Spot-Od as a powerful method to quantify the dynamics of nuclear proteins such as transcription factors (TFs). Here, we provide a protocol for conducting and analyzing SPT experiments with a focus on fast tracking (“fastSPT”) of TFs in mammalian cells. First, we explore how to engineer and prepare cells f作者: Compass 時(shí)間: 2025-3-26 16:35
Characterization of Mammalian Regulatory Complexes at Single-Locus Resolution Using TINCresponsible for cellular identity. Consequently, insight into the molecular composition of these regulatory complexes is of major importance for our understanding of any physiological or pathological cellular state or transition. However, it remains extremely difficult to identify the protein comple作者: organism 時(shí)間: 2025-3-26 16:58
Profiling Protein–DNA Interactions Cell-Type-Specifically with Targeted DamIDolymerase, and chromatin-modifying proteins. The technique is highly sensitive, highly reproducible, requires no mechanical disruption, cell isolation or antibody purification, and can be performed by anyone with basic molecular biology knowledge. Here, we describe the TaDa method and downstream bio作者: 強(qiáng)所 時(shí)間: 2025-3-26 23:54
Genome-Wide Mapping and Microscopy Visualization of Protein–DNA Interactions by pA-DamIDo this list with distinct advantages. pA-DamID relies on antibody-based targeting of the bacterial Dam enzyme, resulting in adenine methylation of DNA in contact with the protein of interest. This .A can then be visualized by microscopy, or mapped genome-wide. The main advantages of pA-DamID are an 作者: tariff 時(shí)間: 2025-3-27 04:06 作者: medium 時(shí)間: 2025-3-27 06:59
High-Resolution ATAC-Seq Analysis of Frozen Clinical TissuesAC-seq analysis of clinical specimens at a single-cell resolution reveals the cellular composition of the tissue contributing to the understanding of intra-tissue heterogeneity. Here we describe our method for nuclei isolation from frozen specimens with wide applicability across tissue types, produc作者: Overstate 時(shí)間: 2025-3-27 11:03
Single-Molecule Multikilobase-Scale Profiling of Chromatin Accessibility Using m6A-SMAC-Seq and m6A-ymatic treatment. This property has been the basis of a number of sequencing-based assays for genome-wide identification and tracking the activity of CREs across different biological conditions, such as DNAse-seq, ATAC-seq, NOMeseq, and others. However, the fragmentation of DNA inherent to many of t作者: GRAZE 時(shí)間: 2025-3-27 13:57 作者: BIDE 時(shí)間: 2025-3-27 18:56 作者: 影響帶來(lái) 時(shí)間: 2025-3-27 22:01
Chromatin978-1-0716-2140-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 移植 時(shí)間: 2025-3-28 05:32 作者: Externalize 時(shí)間: 2025-3-28 08:57 作者: Efflorescent 時(shí)間: 2025-3-28 13:58 作者: 相同 時(shí)間: 2025-3-28 15:10
https://doi.org/10.1007/978-1-0716-2140-0DNAse sensitivity; ChIP +; SMACseq; immunocleavage sequencing; immunoprecipitation作者: Definitive 時(shí)間: 2025-3-28 21:35
978-1-0716-2142-4The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines作者: Bravura 時(shí)間: 2025-3-29 00:55
Chromatin Immunoprecipitation Sequencing (ChIP-seq) Protocol for Small Amounts of Frozen Biobanked Cng histone modifications and DNA–protein interactions. It provides valuable insights to better understand disease mechanisms. Here we present an optimized ChIP-seq protocol suitable for human cardiac tissues, especially the frozen biobanked small biopsy samples.作者: Immunotherapy 時(shí)間: 2025-3-29 04:10
A Robust Protocol for Investigating the Cohesin Complex by ChIP-SequencingCTCF collaborate to form chromatin loops and to gain insight in the intricate regulation of cohesin. Here we describe a detailed ChIP protocol that has been successfully used for different cohesin subunits and cohesin regulators in various cell lines.作者: laxative 時(shí)間: 2025-3-29 09:42
Profiling Protein–DNA Interactions Cell-Type-Specifically with Targeted DamIDolymerase, and chromatin-modifying proteins. The technique is highly sensitive, highly reproducible, requires no mechanical disruption, cell isolation or antibody purification, and can be performed by anyone with basic molecular biology knowledge. Here, we describe the TaDa method and downstream bioinformatics data processing.作者: 集中營(yíng) 時(shí)間: 2025-3-29 13:14 作者: galley 時(shí)間: 2025-3-29 19:26 作者: 對(duì)待 時(shí)間: 2025-3-29 21:32
A Better Democracy, Thanks to MMPation sequencing technology, we can document methylation from many thousands of individual reads (equivalent to alleles or “cells”), for multiple target regions and from many samples simultaneously. Here, we describe a next-generation bisulfite-sequencing assay for targeted DNA methylation analysis 作者: Intact 時(shí)間: 2025-3-30 03:19
Reconstituting the State in Africazed associations between aberrant DNA methylation changes and gene expression, evidence for a causal relationship in this context has been difficult to obtain. Early techniques for interrogating the role of DNA methylation in the regulation of gene transcription lack specificity and, where more spec作者: Narcissist 時(shí)間: 2025-3-30 04:37
State Renewal in Africa: The Lessonse nature of sodium bisulfite results in DNA fragmentation and subsequent biases in sequencing data. Such issues have led to the development of bisulfite-free methods for 5mC detection. Nanopore sequencing is a long read nondestructive approach that directly analyzes DNA and RNA fragments in real tim作者: extinguish 時(shí)間: 2025-3-30 11:17
State Renewal in Africa: The Lessonsng histone modifications and DNA–protein interactions. It provides valuable insights to better understand disease mechanisms. Here we present an optimized ChIP-seq protocol suitable for human cardiac tissues, especially the frozen biobanked small biopsy samples.作者: 初學(xué)者 時(shí)間: 2025-3-30 14:27 作者: FADE 時(shí)間: 2025-3-30 18:00
https://doi.org/10.1057/978-1-137-58782-4nd their complexes have been identified before, but how each genomic locus interacts with its surrounding proteins in the nucleus over time and in changing conditions remains poorly described. Measuring protein–DNA interactions at a specific locus in the genome is challenging and current techniques 作者: 甜得發(fā)膩 時(shí)間: 2025-3-30 23:37
‘Education’ and the Pruning of the Mindd as a powerful method to quantify the dynamics of nuclear proteins such as transcription factors (TFs). Here, we provide a protocol for conducting and analyzing SPT experiments with a focus on fast tracking (“fastSPT”) of TFs in mammalian cells. First, we explore how to engineer and prepare cells f
