標題: Titlebook: Chemosensitivity; Volume II: In Vivo M Rosalyn D. Blumenthal Book 2005 Humana Press 2005 DNA.apoptosis.cell.cell death.chemoresistance.geno [打印本頁] 作者: ODE 時間: 2025-3-21 16:35
書目名稱Chemosensitivity影響因子(影響力)
書目名稱Chemosensitivity影響因子(影響力)學科排名
書目名稱Chemosensitivity網(wǎng)絡公開度
書目名稱Chemosensitivity網(wǎng)絡公開度學科排名
書目名稱Chemosensitivity被引頻次
書目名稱Chemosensitivity被引頻次學科排名
書目名稱Chemosensitivity年度引用
書目名稱Chemosensitivity年度引用學科排名
書目名稱Chemosensitivity讀者反饋
書目名稱Chemosensitivity讀者反饋學科排名
作者: 導師 時間: 2025-3-21 21:09
Immunodetecting Members of the Bcl-2 Family of Proteinsacellular levels of multiple members of the Bcl-2 protein family can be analyzed simultaneously by immunoblotting and how protein-protein interactions can be studied using immunoprecipitation techniques. In addition, the methodology describes how conformational changes can be observed by immunofluor作者: instructive 時間: 2025-3-22 01:39
Correlation of Telomerase Activity and Telomere Length to Chemosensitivityerapeutic intervention. Although a telomerase-specific inhibitor has not been found yet, the possible effect of anticancer agents on telomerase inhibition or the alteration of telomere length has been proposed. Recent development of TRAP assay not only increased the sensitivity but also allowed fast作者: Dendritic-Cells 時間: 2025-3-22 08:05 作者: CRACK 時間: 2025-3-22 10:49
Overview of Tumor Cell Chemoresistance Mechanismsression. The relevant mechanisms that can contribute to cellular resistance include: increased expression of defense factors involved in reducing intracellular drug concentration; alterations in drug-target interaction; and changes in cellular response, in particular increased cell ability to repair作者: debunk 時間: 2025-3-22 14:04
Flow Cytometric Monitoring of Fluorescent Drug Retention and Effluxnce in the presence or absence of an efflux blocker such as verapamil. The present chapter discusses some of the flow cytometric methods used for the study of cellular drug retention and the artifacts that may arise in such analysis.作者: debunk 時間: 2025-3-22 18:02
Limit Cycles and Structural Stability,tic damage, screening of chemicals for genotoxic potential, and for specific purposes such as prediction of the radiosensitivity of tumors and interindividual variation in radiosensi tivity. In its current basic form the CBMN assay can provide, using simple morphological crite ria, the following mea作者: dagger 時間: 2025-3-22 22:41
https://doi.org/10.1007/978-3-540-33308-1acellular levels of multiple members of the Bcl-2 protein family can be analyzed simultaneously by immunoblotting and how protein-protein interactions can be studied using immunoprecipitation techniques. In addition, the methodology describes how conformational changes can be observed by immunofluor作者: 沒有貧窮 時間: 2025-3-23 05:06
Bj?rn Rasch,Wilhelm Hofmann,Ewald Naumannerapeutic intervention. Although a telomerase-specific inhibitor has not been found yet, the possible effect of anticancer agents on telomerase inhibition or the alteration of telomere length has been proposed. Recent development of TRAP assay not only increased the sensitivity but also allowed fast作者: climax 時間: 2025-3-23 06:25
Varianzanalyse mit Messwiederholung,tion. Basically, three procedures are described: (1) tissue dissociation and further cultivation on the sensor chip or on Transwell inserts; (2) preparation of tissue slices (300 μm thick) and attachment to the sensor chip or to inserts, and (3) cultivation of cells in dialysis tubes, a procedure ne作者: 以煙熏消毒 時間: 2025-3-23 09:41 作者: 賠償 時間: 2025-3-23 16:42 作者: TSH582 時間: 2025-3-23 21:47 作者: Inferior 時間: 2025-3-24 01:25 作者: 少量 時間: 2025-3-24 03:17
Bj?rn Rasch,Wilhelm Hofmann,Ewald Naumannapoptosis following chemotherapy by TUNEL in cells in culture and in tissue biopsied from patients or laboratory animals that received chemotherapy. It lists the reagents, pro cedures for cell and tissue handling, labeling techniques, caveats, and appropriate controls for labeling 3′OH termini with TdT and quantifying the results.作者: 上坡 時間: 2025-3-24 08:41
Springer Series in Chemical Physicsere we focus on methods developed in fission yeast with particular reference to gene disruption, transformation, and mutagenesis approaches. These methods could be useful in an attempt to develop target-specific therapeutic strategies.作者: FRET 時間: 2025-3-24 11:45 作者: 未成熟 時間: 2025-3-24 17:54 作者: Arthropathy 時間: 2025-3-24 21:00
1543-1894 vitro and in vivo, and for assessing the parameters that modulate chemosensitivity in individual tumors. Chemosensitivity,Volume 2: In Vivo Models, Imaging, and Molecular Regulators contains cutting-edge protocols for classifying tumors into response categories and for customizing therapy to individ作者: Conduit 時間: 2025-3-24 23:50 作者: FLIC 時間: 2025-3-25 05:30
Measurement of Ceramide and Sphingolipid Metabolism in Tumorsnvestigating the mode of action of antitumor drugs. This chapter summarizes protocols for quantitating the level of apoptosis, the activities of acidic sphingomyelinase, neutral sphingomyelinase, glycosylceramide synthase, sphingomyelin synthase, and ceramidase, and the amount of ceramide in tumor xenografts in nude mice.作者: reflection 時間: 2025-3-25 10:34 作者: 抓住他投降 時間: 2025-3-25 14:52 作者: 未開化 時間: 2025-3-25 19:14
Polynomial Planar Phase Portraits,cycle, followed by induction of cell death by anticancer drugs. We also describ three methods (flow cytometry, Western blot, and DNA fragmentation) to measure the cell cycle-associated cytotoxic effects of chemotherapeutic agents.作者: 惡意 時間: 2025-3-25 20:14
Bj?rn Rasch,Wilhelm Hofmann,Ewald Naumannolution of high salt, detergents, and alkali. DNA was precipitated in microgels by ethanol and spermine. Staining of DNA was done with an intense fluorescent dye, YOYO-1. Apoptotic cells show a circular gradient of granular DNA with a dense central zone and a lighter and hazy outer zone, giving the overall appearance of a halo.作者: laxative 時間: 2025-3-26 02:47
https://doi.org/10.1007/978-3-540-33308-1thway. Diphenylamine assay enables the quantitation of degraded DNA. The protocol is simple and allows manipulation of all types of cell cultures. Resulting colorimetric reaction is easily quantitated, and the assay is highly reproducible.作者: 值得尊敬 時間: 2025-3-26 05:13
Bj?rn Rasch,Malte Friese,Ewald Naumanncute Myeloid Leukaemia and High Risk Myelodysplastic Syndrome (?10% blasts). These assays are performed in more than one UK Centre, and hence each assay has been subjected to rigorous reproducibility testing (.).作者: 成績上升 時間: 2025-3-26 11:20 作者: 豎琴 時間: 2025-3-26 16:40
Apoptosis Assessment by the DNA Diffusion Assayolution of high salt, detergents, and alkali. DNA was precipitated in microgels by ethanol and spermine. Staining of DNA was done with an intense fluorescent dye, YOYO-1. Apoptotic cells show a circular gradient of granular DNA with a dense central zone and a lighter and hazy outer zone, giving the overall appearance of a halo.作者: harmony 時間: 2025-3-26 19:17 作者: cardiopulmonary 時間: 2025-3-26 23:25
Flow Cytometric Measurement of Functional and Phenotypic P-Glycoproteincute Myeloid Leukaemia and High Risk Myelodysplastic Syndrome (?10% blasts). These assays are performed in more than one UK Centre, and hence each assay has been subjected to rigorous reproducibility testing (.).作者: 頭盔 時間: 2025-3-27 03:13
Book 2005rameters that modulate chemosensitivity in individual tumors. Chemosensitivity,Volume 2: In Vivo Models, Imaging, and Molecular Regulators contains cutting-edge protocols for classifying tumors into response categories and for customizing therapy to individuals. These readily reproducible techniques作者: Malfunction 時間: 2025-3-27 07:07
https://doi.org/10.1007/978-3-540-33308-1tect procaspases and caspases and their activity. Immunodetection and fluorescent substrates are widely accepted methods that are utilized for this purpose. These methods are described in detail in this chapter.作者: 實施生效 時間: 2025-3-27 11:43
https://doi.org/10.1007/978-3-540-33310-4nvestigating the mode of action of antitumor drugs. This chapter summarizes protocols for quantitating the level of apoptosis, the activities of acidic sphingomyelinase, neutral sphingomyelinase, glycosylceramide synthase, sphingomyelin synthase, and ceramidase, and the amount of ceramide in tumor xenografts in nude mice.作者: 同步左右 時間: 2025-3-27 14:44
Springer Series in Chemical Physicses are used as test examples to predict their associated mechanistic categories. This method of correlating drugs and genes provides a strategy for finding novel biologically significant relationships for molec ular pharmacology.作者: 哭得清醒了 時間: 2025-3-27 21:21 作者: 好色 時間: 2025-3-28 00:15 作者: 膽小鬼 時間: 2025-3-28 02:39 作者: FLUSH 時間: 2025-3-28 07:47 作者: 使虛弱 時間: 2025-3-28 11:58
Rosalyn D. BlumenthalIncludes supplementary material: 作者: 莎草 時間: 2025-3-28 16:54
Methods in Molecular Medicinehttp://image.papertrans.cn/c/image/224938.jpg作者: Collision 時間: 2025-3-28 22:20
Limit Cycles and Structural Stability,nogenesis. The micronucleus assays have emerged as one of the preferred methods for assessing chromosome damage because they enable both chromosome loss and chromosome breakage to be measured reliably. Because micronuclei can only be expressed in cells that complete nuclear division a special method作者: DEBT 時間: 2025-3-29 00:11 作者: Infirm 時間: 2025-3-29 04:38
Bj?rn Rasch,Wilhelm Hofmann,Ewald Naumanncan be a useful tool to understanding the mechanisms of action of chemotherapy agents, drug resistance, and tumor biology. Cells undergoing apoptosis eventually cleave their DNA with nucleases in a character istic pattern that leaves free 3′OH ends. This permits the identification of cells undergoin作者: 沉著 時間: 2025-3-29 08:35 作者: MIRTH 時間: 2025-3-29 14:58 作者: 難理解 時間: 2025-3-29 18:23 作者: Generosity 時間: 2025-3-29 21:49 作者: Concomitant 時間: 2025-3-30 01:21 作者: 樂意 時間: 2025-3-30 06:39 作者: PAC 時間: 2025-3-30 10:54 作者: confederacy 時間: 2025-3-30 15:28
https://doi.org/10.1007/978-3-540-33310-4n) and fluorochromes used for the detection of cellular drug efflux and resistance (e.g., rhodamine 123, Hoechst 33342). Multi parametric flow cytometry can be used for identification of tumor cell subpopulations based on their drug retention profiles with or without the presence of an efflux blocke作者: Brocas-Area 時間: 2025-3-30 16:37
Bj?rn Rasch,Malte Friese,Ewald Naumann blood and bone marrow cells. We describe a functional assay for P-glycoprotein using rhodamine 123, an assay for daunorubicin accumulation, and an assay to measure P-glycoprotein levels using the MRK16 antibody. Our protocols include the use of an anti-CD45 antibody for the identification of leukem作者: eustachian-tube 時間: 2025-3-30 20:51
https://doi.org/10.1007/978-3-540-33310-4iosides, and sphingosine. Ceramide production is increased in response to stress and toxic agents. Because modulation of ceramide levels has been shown to affect sensitivity and/or resistance to therapeutic agents, it will be important to assess the activity of sphingolipid metabolic pathways when i作者: Deference 時間: 2025-3-31 04:02
Bj?rn Rasch,Malte Friese,Ewald Naumannotocol includes steps for drug exposure, RNA isolation, preparation of fluorescently labeled samples, microarray hybridization, data pro cessing, and data analysis. In addition to the detailed protocol, important experimental design issues are discussed, and some preliminary experiments are recommen作者: Manifest 時間: 2025-3-31 07:20
Springer Series in Chemical Physics the same mechanism, a supervised machine learning approach called support vector machine (SVM) is used to associate genes with any of five predefined anticancer drug mechanistic categories. The drug activity profiles are used as training examples to train the SVM and then the gene expression profil作者: GROUP 時間: 2025-3-31 09:50
Springer Series in Chemical Physicsmodel systems for the identification of determinants of chemosensitivity. Indeed, yeast mutants of DNA repair and cell cycle checkpoint pathways exhibit increased sensitivity to selected antitumor drugs, thereby allowing us to establish the role of specific genes in drug response. The basic cellular作者: habitat 時間: 2025-3-31 16:43
