標題: Titlebook: Chemokine Protocols; Amanda E. I. Proudfoot,Timothy N. C. Wells,Christi Book 2000 Humana Press 2000 [打印本頁] 作者: breath-focus 時間: 2025-3-21 16:31
書目名稱Chemokine Protocols影響因子(影響力)
書目名稱Chemokine Protocols影響因子(影響力)學科排名
書目名稱Chemokine Protocols網(wǎng)絡(luò)公開度
書目名稱Chemokine Protocols網(wǎng)絡(luò)公開度學科排名
書目名稱Chemokine Protocols被引頻次
書目名稱Chemokine Protocols被引頻次學科排名
書目名稱Chemokine Protocols年度引用
書目名稱Chemokine Protocols年度引用學科排名
書目名稱Chemokine Protocols讀者反饋
書目名稱Chemokine Protocols讀者反饋學科排名
作者: obeisance 時間: 2025-3-21 20:22 作者: Yourself 時間: 2025-3-22 00:30
Chemokine Expression in Insect Cells,ay be predicted from deduced amino acid sequence, a rigorous proof of identity of a gene will require a demonstration of the appropriate biological activities of gene products. Thus, efficient expression of recombinant proteins of cloned genes is essential to obtain proteins sufficient for detailed 作者: 周興旺 時間: 2025-3-22 05:49 作者: 草率男 時間: 2025-3-22 08:54
Expression of Chemokines in the Periplasmic Space of E. coli,ations, in the case of RANTES, and with a yield of several hundred micrograms from a 1-L culture. Other expression systems that are generally used for the production of chemokines have several disadvantages. Bacterial expression in the cytoplasm of . (.,.) gives large yields, but sometimes leads to 作者: Atrium 時間: 2025-3-22 16:33
Synthesis of Chemokines,ning has resulted in an explosion in the number of new chemokines from 1995-1998. However, for studies of the protein, knowing the DNA sequence is only the first step. The chemokine must be generated and in its correctly processed and folded form, and then purified to homogeneity. Expression of the 作者: Atrium 時間: 2025-3-22 17:44
Identification of Novel Chemokines From Expressed Sequence Tag Databases,hree-dimensional subunit structure. The original members of the superfamily, Interleukin-8 and MCP-1 were purified over 10 years ago. Following on from these discoveries, many more of these proteins were identified because of their ability to selectively recruit and activate specific leukocyte popul作者: EWER 時間: 2025-3-23 00:49 作者: 高度 時間: 2025-3-23 04:10 作者: 有法律效應(yīng) 時間: 2025-3-23 06:10
Generation of Stable Cell Lines Expressing Chemokine Receptors, expressing specific chemokine receptors greatly facilitates chemokine receptor characterization, particularly in terms of ligand binding specificity, analysis of signal transduction pathways, and for diverse functional assays ranging from chemotaxis to HIV infection. The availability of stable cell作者: defile 時間: 2025-3-23 11:33
Modified Microchemotaxis Assays,motactic molecules. Upon encountering a chemotactic molecule, responding leukocytes begin to migrate directionally from regions of low ligand concentrations toward the sites of chemoattractant production that typically possess more substantial levels of soluble chemotactic factors. A number of endog作者: FOLD 時間: 2025-3-23 14:43
Transwell Chemotaxis,in activation, chemotaxis, lipid mediator biosynthesis, superoxide radical production, and granule enzyme release (reviewed in ..–.), chemokines have been shown to suppress and stimulate angiogenesis (.–.), suppress hematopoiesis (.–.), suppress apoptosis (.), control viral infection (.,.), and effe作者: Aggregate 時間: 2025-3-23 22:04 作者: pericardium 時間: 2025-3-23 23:54
Radiolabeled Chemokine Binding Assays,ine system represents a striking example as most receptors in the family bind multiple chemokines, often with complex overlapping selectivities. Two basic methodologies are used to characterize the binding properties of a receptor. In saturation protocols, the binding of a given ligand, usually labe作者: 無能性 時間: 2025-3-24 03:13
Scintillation Proximity Binding Assay,urthermore, receptor-ligand binding assays are used extensively for drug screening. The Scintillation Proximity Assay (SPA) is now a well established screening technology and a considerable number of SPA based receptor screening assays have been run by pharmaceutical companies. This homogeneous radi作者: 廚房里面 時間: 2025-3-24 08:25
Calcium Mobilization,gen, sodium, and potassium concentrations. It is generally recognized that cells spend a great amount of their energy on stringent regulation of the level of cytosolic free Ca.+, and that Ca.+ plays a very important regulatory role in cellular functions作者: 6Applepolish 時間: 2025-3-24 13:11
Actin Polymerization, of cells into the inflamed tissue, they have to pass the endothelium. This process involves several types of adhesion molecule interactions between eosinophils and endothelial cells. Selectins mediate two steps, initial tethering to the vessel wall and their subsequent rolling. Integrins then bind 作者: CLEAR 時間: 2025-3-24 16:37 作者: 出血 時間: 2025-3-24 20:51
Histamine Release,tory role in immediate hypersensitivity reactions. Some of the mediators are preformed and stored in granules. An example is histamine. Other mediators are generated ., e.g., eicosanoids. Mast cells and basophils produce some important cytokines, e.g., IL-4, IL-13, TNF-α (.–.) and the chemokines MlP作者: 睨視 時間: 2025-3-25 00:51 作者: Clumsy 時間: 2025-3-25 05:52 作者: Jargon 時間: 2025-3-25 08:52
Chemokine Expression in Insect Cells,tivities of gene products. Thus, efficient expression of recombinant proteins of cloned genes is essential to obtain proteins sufficient for detailed biological and biochemical analysis. In this chapter, I will describe a rapid and efficient method to generate recombinant chemokines in insect cells (.,.).作者: Mitigate 時間: 2025-3-25 13:24
Purification of Chemokines from Natural Sources,walking strategy, we have discovered an ever-increasing number of novel genes encoding chemokines that were expressed in bacteria or eukaryotic cells and subsequently tested as the recombinant form for its biological activity.作者: cushion 時間: 2025-3-25 19:14
Cloning of Novel Chemokines Using a Signal Sequence Trap Method,tively clone cDNA species encoding intercellular signal-transducing molecules without biologic assays. In this chapter, an efficient signal sequence trap method based on an Epstein-Barr virus shuttle vector is described (.).作者: DEMN 時間: 2025-3-25 21:33
Chemokine Receptor Cloning,to bind chemokines (.). Reverse methods, which involve cloning a cDNA or gene without primary knowledge of the structure of the encoded protein, can be divided into two types: expression and homology hybridization cloning.作者: 書法 時間: 2025-3-26 01:07
Generation of Stable Cell Lines Expressing Chemokine Receptors, lines expressing high levels of chemokine receptors is also becoming increasingly important in high throughput screening approaches for the identification of chemokine receptor antagonists and agonists. The basic steps in the generation of stable cell lines expressing chemokine receptors are as follows:作者: Resign 時間: 2025-3-26 07:01
Transwell Chemotaxis,ct leukocyte differentiation (.). Among the proinflammatory activities, chemotaxis in particular has received considerable attention as a target for novel antiinflammatory therapeutics (reviewed in ..).作者: CLAY 時間: 2025-3-26 11:38
Actin Polymerization,to endothelial receptors, increasing adhesion which results in the arrest of the rolling leukocytes, which can then cross the endothelial layer of the blood vessel and enter the tissue using integrins for traction (.–.).作者: lipids 時間: 2025-3-26 12:48 作者: Adulate 時間: 2025-3-26 17:09 作者: 恩惠 時間: 2025-3-26 21:27
Svetlana G. Lukishova,Luke J. Bissellfide, and the CX.C chemokine neurotactin or fraktalkine which has three amino acids between the first cysteine pair, both adopt the same fold. Modeling in fact shows that either one or three residues between the first two Cys can be adopted, but not two, probably explaining why examples of CX.C chemokines do not exist.作者: 北京人起源 時間: 2025-3-27 01:24
https://doi.org/10.1007/978-3-319-99798-8ion of leaky blood vessels. In other pathological states, such as cancer, the tumor itself appears to tightly regulate new vessel formation which results in its maintenance, and often continued growth.作者: 無瑕疵 時間: 2025-3-27 07:37
Expression of Chemokines in the Periplasmic Space of E. coli,xpression gives rise to correctly folded proteins, but has low yields, and secreted chemokines are often subject to proteolysis. In the case of RANTES expressed in CHO cells, a high proportion of the protein lacks the first two or three N-terminal amino acids.作者: enchant 時間: 2025-3-27 11:36
Purification of Recombinant Chemokines from ,,fide, and the CX.C chemokine neurotactin or fraktalkine which has three amino acids between the first cysteine pair, both adopt the same fold. Modeling in fact shows that either one or three residues between the first two Cys can be adopted, but not two, probably explaining why examples of CX.C chemokines do not exist.作者: 和藹 時間: 2025-3-27 16:14 作者: 背書 時間: 2025-3-27 20:29 作者: 擁護 時間: 2025-3-27 22:31
Owoicho Adogwa,Joseph Cheng,John E. O’Tooletively clone cDNA species encoding intercellular signal-transducing molecules without biologic assays. In this chapter, an efficient signal sequence trap method based on an Epstein-Barr virus shuttle vector is described (.).作者: Heart-Rate 時間: 2025-3-28 04:24
Svetlana G. Lukishova,Luke J. Bissellto bind chemokines (.). Reverse methods, which involve cloning a cDNA or gene without primary knowledge of the structure of the encoded protein, can be divided into two types: expression and homology hybridization cloning.作者: Cholecystokinin 時間: 2025-3-28 06:16
A Short Introduction to Survey Research, lines expressing high levels of chemokine receptors is also becoming increasingly important in high throughput screening approaches for the identification of chemokine receptor antagonists and agonists. The basic steps in the generation of stable cell lines expressing chemokine receptors are as follows:作者: 留戀 時間: 2025-3-28 12:40 作者: aspersion 時間: 2025-3-28 17:53
Soziologisch-theoretische Voraussetzungen,to endothelial receptors, increasing adhesion which results in the arrest of the rolling leukocytes, which can then cross the endothelial layer of the blood vessel and enter the tissue using integrins for traction (.–.).作者: 偏見 時間: 2025-3-28 20:11
,Qualit?tspolitik und Qualit?tswirtschaft,.), with NADPH serving as the electron donor. In the presence of eosinophil peroxidase, H.O. may then give rise to the potentially cytotoxic hypohalous acids such as HOBr. The increase in oxygen consumption is termed the respiratory burst (.,.).作者: elucidate 時間: 2025-3-29 00:28
A Short Introduction to Survey Research,tokines (e.g., IL-1, TNFα, IFN-γ), and neuroendocrine hormones (.). Although many of these factors are believed to play some role in inflammation, their relevance to leukocyte infiltration into inflammatory sites or homing to lymphoid organs has been brought into question.作者: helper-T-cells 時間: 2025-3-29 03:36 作者: MOCK 時間: 2025-3-29 09:52 作者: TIA742 時間: 2025-3-29 14:18
,Die Qualit?t in der Kostentheorie,activation mechanism basophils respond to a wide variety of stimuli. They include C5a, C3a, FMLP, hyperosmolar solutions, neuropeptides, histamine releasing factor, and CC chemokines (.). The most potent basophil-activating CC chemokines are MCP-1, MCP-3, MCP-4, eotaxin, RANTES, and MlP-lα (.).作者: Repetitions 時間: 2025-3-29 16:13 作者: obsolete 時間: 2025-3-29 23:22
Radiolabeled Chemokine Binding Assays,ng concentrations of an unlabeled ligand to inhibit the binding of a fixed concentration of a labeled ligand. Although saturation protocols have the advantage of theoretical simplicity, they have three significant disadvantages:作者: 外貌 時間: 2025-3-30 02:56 作者: 斥責 時間: 2025-3-30 04:26 作者: amenity 時間: 2025-3-30 11:22
Book 2000afficking to the extravasation and tissue infiltration of leukocytes in response to inflammatory agents, tissue damage, and bacterial or viral infection. Chemokines exert their effects through a family of seven G-protein coupled transmembrane receptors. Worldwide interest in the chemokine field surg作者: 浮雕 時間: 2025-3-30 14:09 作者: 縮影 時間: 2025-3-30 18:42 作者: Gesture 時間: 2025-3-30 23:49 作者: NADIR 時間: 2025-3-31 04:25 作者: 行業(yè) 時間: 2025-3-31 07:03 作者: Fibrillation 時間: 2025-3-31 13:03
Queen Caroline and Sir William Gellning has resulted in an explosion in the number of new chemokines from 1995-1998. However, for studies of the protein, knowing the DNA sequence is only the first step. The chemokine must be generated and in its correctly processed and folded form, and then purified to homogeneity. Expression of the 作者: fertilizer 時間: 2025-3-31 15:14 作者: Homocystinuria 時間: 2025-3-31 18:58
Svetlana G. Lukishova,Luke J. Bissellvel of homology at the primary sequence level, which can be as low as 20%, although it can also be as high as 90%, the three-dimensional structure of all the chemokines solved to date has shown that they have a superimposable monomeric fold. This fold is imposed on the family by the four cysteine mo
