標題: Titlebook: Cellular Quiescence; Methods and Protocol H. Daniel Lacorazza Book 2018 Springer Science+Business Media, LLC, part of Springer Nature 2018 [打印本頁] 作者: VIRAL 時間: 2025-3-21 19:12
書目名稱Cellular Quiescence影響因子(影響力)
書目名稱Cellular Quiescence影響因子(影響力)學(xué)科排名
書目名稱Cellular Quiescence網(wǎng)絡(luò)公開度
書目名稱Cellular Quiescence網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Cellular Quiescence被引頻次
書目名稱Cellular Quiescence被引頻次學(xué)科排名
書目名稱Cellular Quiescence年度引用
書目名稱Cellular Quiescence年度引用學(xué)科排名
書目名稱Cellular Quiescence讀者反饋
書目名稱Cellular Quiescence讀者反饋學(xué)科排名
作者: patriarch 時間: 2025-3-21 23:11
Pathological Conditions of the Vocal Foldby density gradient. We also describe approaches for DAPI staining the chromatin of quiescent cells, measuring quiescent cell viability, and extracting RNA from quiescent cells for use in genomics experiments.作者: 描繪 時間: 2025-3-22 03:27
https://doi.org/10.1007/978-1-4939-7371-2Cellular dormancy; Stem cells; Prokaryotes; Eukaryotes; Genomic regulation作者: 燈泡 時間: 2025-3-22 08:00
978-1-4939-8465-7Springer Science+Business Media, LLC, part of Springer Nature 2018作者: 禮節(jié) 時間: 2025-3-22 10:09
Anatomy and Physiology of the Larynxve the same amount of DNA as cells at G1 phase but lower RNA levels compared to proliferating cells. Therefore, resting cells in G0 phase can be distinguished from proliferating cells in G1, S, and G2?M phases. This chapter describes a protocol for double staining of live cells with Hoechst 33342 an作者: arboretum 時間: 2025-3-22 14:26 作者: arboretum 時間: 2025-3-22 20:37
Anatomy and Physiology of the Larynxonal precursors in the adult mammalian brain. Various approaches of fluorescent-activated cell sorting (FACS) have emerged to allow the distinction between quiescent NSCs (qNSCs), their activated counterpart (aNSCs), and the resulting progeny. In this article, we review two FACS techniques that can 作者: 暴露他抗議 時間: 2025-3-22 21:43 作者: 倒轉(zhuǎn) 時間: 2025-3-23 03:05
Perioperative Care for Phonomicrosurgeryical in many studies. However, measuring the proliferation rate of largely quiescent and rare populations of cells can be challenging. Bromo-deoxyuridine (BrdU) incorporation into replicating DNA is a commonly used and highly reproducible method to detect cell division history. Here, we describe a p作者: Annotate 時間: 2025-3-23 09:15 作者: 大罵 時間: 2025-3-23 11:14
Pathological Conditions of the Vocal Foldby density gradient. We also describe approaches for DAPI staining the chromatin of quiescent cells, measuring quiescent cell viability, and extracting RNA from quiescent cells for use in genomics experiments.作者: deceive 時間: 2025-3-23 17:33 作者: 使更活躍 時間: 2025-3-23 19:57 作者: 責(zé)任 時間: 2025-3-24 01:04 作者: Awning 時間: 2025-3-24 05:06 作者: 是貪求 時間: 2025-3-24 09:59
Pathological Conditions of the Vocal Foldycle activity in murine bone marrow during inflammation is often complicated by the relative rarity of HSCs and shifts in Sca-1, a key cell surface marker used to identify HSCs. Here, we report a method to analyze HSC proliferation and cell cycle distribution under inflammatory conditions. Our appro作者: 侵害 時間: 2025-3-24 11:35 作者: 責(zé)任 時間: 2025-3-24 16:37 作者: 顧客 時間: 2025-3-24 21:52 作者: Relinquish 時間: 2025-3-25 02:49 作者: Progesterone 時間: 2025-3-25 07:23 作者: JUST 時間: 2025-3-25 10:25 作者: bifurcate 時間: 2025-3-25 12:24 作者: 珍奇 時間: 2025-3-25 18:50
Preparation and Analysis of , Quiescent Cells,by density gradient. We also describe approaches for DAPI staining the chromatin of quiescent cells, measuring quiescent cell viability, and extracting RNA from quiescent cells for use in genomics experiments.作者: Intrepid 時間: 2025-3-25 22:53
Book 2018d how cells exit quiescence and re-enter differentiating cell divisions to restore damaged tissues, essential for developing new approaches in regenerative medicine in the future. The chapters in this book were designed to address cellular quiescence in prokaryote and eukaryote organisms, detection 作者: 彎彎曲曲 時間: 2025-3-26 02:26 作者: Mirage 時間: 2025-3-26 06:04 作者: Frisky 時間: 2025-3-26 11:06 作者: perpetual 時間: 2025-3-26 15:01 作者: 無所不知 時間: 2025-3-26 17:23
Single EDL Myofiber Isolation for Analyses of Quiescent and Activated Muscle Stem Cells,, we describe a protocol to isolate single myofibers from the . muscle. Moreover, we detail experimental conditions for analyzing satellite cells in quiescence and progression through the myogenic lineage.作者: maladorit 時間: 2025-3-26 22:34
Investigating Cellular Quiescence of T Lymphocytes and Antigen-Induced Exit from Quiescence,ent regulation of quiescence has also been implicated in the differentiation and function of memory T cells. In this chapter, we describe techniques to assess quiescent state of na?ve T cells under steady state and exit from quiescence upon TCR stimulation.作者: 不在灌木叢中 時間: 2025-3-27 03:40 作者: osteopath 時間: 2025-3-27 08:31
Pathological Conditions of the Vocal Foldong-lived GFP label retaining cells. This method is free from confounding factors of previous methodologies based on radioactive tracers and also enables functional studies not previously possible using the radioactive tracer techniques described in the literature.作者: uveitis 時間: 2025-3-27 11:08 作者: Cardioversion 時間: 2025-3-27 17:25
Anatomy and Physiology of the Larynxed tet-regulatable transgenic mouse model can be used to express histone H2B-GFP in epithelial proliferative cells and their dilution and retention of the GFP signal can be followed. In this chapter, we detail the steps to perform BrdU pulse-chase and H2B-GFP pulse-chase experiments to identify quiescent cells in the hair follicle.作者: 輕率看法 時間: 2025-3-27 20:47 作者: 節(jié)約 時間: 2025-3-28 00:37 作者: Palpitation 時間: 2025-3-28 04:41
Cell Cycle Analysis by Mass Cytometry,es. The method utilizes incorporation of 5-Iodo-2′-deoxyuridine (IdU), combined with measurement of phosphorylated retinoblastoma protein (pRb), cyclin B1, and phosphorylated histone H3 (p-HH3). These measurements can be integrated into a gating strategy that allows for clear separation of all five phases of the cell cycle.作者: 下邊深陷 時間: 2025-3-28 08:30
Identifying Quiescent Stem Cells in Hair Follicles,ed tet-regulatable transgenic mouse model can be used to express histone H2B-GFP in epithelial proliferative cells and their dilution and retention of the GFP signal can be followed. In this chapter, we detail the steps to perform BrdU pulse-chase and H2B-GFP pulse-chase experiments to identify quiescent cells in the hair follicle.作者: 尖酸一點 時間: 2025-3-28 10:36
A Facile, In Vitro 384-Well Plate System to Model Disseminated Tumor Cells in the Bone Marrow Micros. We successfully screened for compounds that selectively eliminated cancer cells versus supportive stromal cells and verified results with comparison to efficacy in vivo. The spheroid coculture system successfully modeled key aspects of DTCs in the bone marrow microenvironment, facilitating testing for compounds to selectively eliminate DTCs.作者: finale 時間: 2025-3-28 15:33
Flow Cytometric Detection of G0 in Live Cells by Hoechst 33342 and Pyronin Y Staining,d Pyronin Y. Combined with immunophenotyping of intact and live cells Hoechst 33342 and Pyronin Y staining is a powerful noninvasive method for the analysis and isolation of quiescent cells from any defined cell population.作者: Bernstein-test 時間: 2025-3-28 20:23 作者: Foolproof 時間: 2025-3-29 02:05 作者: RAGE 時間: 2025-3-29 05:11 作者: scrutiny 時間: 2025-3-29 10:42
Analysis of lncRNA-Protein Interactions by RNA-Protein Pull-Down Assays and RNA Immunoprecipitationecipitated with a protein of interest. Both the methods can be applied to material from proliferating and quiescent cells, thus providing insights into how lncRNA-protein interactions are altered between these two cellular states.作者: 思想上升 時間: 2025-3-29 12:21 作者: 等級的上升 時間: 2025-3-29 19:27 作者: Apoptosis 時間: 2025-3-29 20:11
Perioperative Care for Phonomicrosurgeryrotocol for BrdU incorporation analysis in hematopoietic stem and progenitor cells that can provide a sensitive measure of cell division even in rare cell populations. In combination with flow cytometry, this method can be generalized to analyze other cell populations and other tissues as identified by cell surface markers.作者: ABIDE 時間: 2025-3-30 01:37
Pathological Conditions of the Vocal Foldach uses EdU incorporation and Ki67 staining coupled with DNA content quantification by DAPI. We also incorporate the surface marker ESAM to help minimize the potential for contaminating events that may confound analysis in the HSC compartment.作者: 人類 時間: 2025-3-30 07:27
The Handbook of Environmental Chemistryecipitated with a protein of interest. Both the methods can be applied to material from proliferating and quiescent cells, thus providing insights into how lncRNA-protein interactions are altered between these two cellular states.作者: 大猩猩 時間: 2025-3-30 11:57 作者: 來這真柔軟 時間: 2025-3-30 14:19
Molecular Regulation of Cellular Quiescence: A Perspective from Adult Stem Cells and Its Niches,作者: 對待 時間: 2025-3-30 18:34 作者: 群居動物 時間: 2025-3-30 21:48
Using Carboxy Fluorescein Succinimidyl Ester (CFSE) to Identify Quiescent Glioblastoma Stem-Like Ceuorescein succinimidyl ester (CFSE) staining and flow cytometric analysis. Quiescent glioblastoma cells with stem-like features are characterized and subsequently isolated based on their ability to retain the CFSE labeling.作者: Irascible 時間: 2025-3-31 01:14
Distinguishing States of Arrest: Genome-Wide Descriptions of Cellular Quiescence Using ChIP-Seq andand loss of hallmarks of quiescence. Thus, in vitro models that recapitulate the dynamic reversibly arrested stem cell state in culture and lend themselves to comparison with the activated or differentiated state are useful templates for genome-wide analysis of the quiescence network..In this chapte作者: SIT 時間: 2025-3-31 05:01
Analysis of MicroRNA-Mediated Translation Activation of In Vitro Transcribed Reporters in QuiescentG0 leukemic cells. Nucleofection of a microRNA target reporter and either cognate, targeting microRNA, or control microRNA, into the nucleus of G0 cells, enables analysis of translation activation by microRNAs in G0. We discuss a modified protocol that we developed for transfection of mRNAs along wi作者: Confound 時間: 2025-3-31 13:10
Genome-Wide Identification of Transcription Factor-Binding Sites in Quiescent Adult Neural Stem Cel the genome can be obtained by assessing DNA-protein interaction through next-generation chromatin immunoprecipitation sequencing technology (ChIP-seq). This chapter outlines the protocol to perform, analyze, and validate ChIP-seq experiments that can be used to identify protein-DNA interactions and作者: 殺子女者 時間: 2025-3-31 17:23
1064-3745 ndtips on troubleshooting and avoiding known pitfalls..Authoritative and practical,?.Cellular Quiescence: Methods and Protocols.?offers a broad view of basic and cutting-edge technology to inspire research in t978-1-4939-8465-7978-1-4939-7371-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 書法 時間: 2025-3-31 19:07
Anatomy and Physiology of the Larynxuorescein succinimidyl ester (CFSE) staining and flow cytometric analysis. Quiescent glioblastoma cells with stem-like features are characterized and subsequently isolated based on their ability to retain the CFSE labeling.作者: Enervate 時間: 2025-3-31 23:02 作者: impale 時間: 2025-4-1 02:14 作者: Infuriate 時間: 2025-4-1 09:20 作者: 委屈 時間: 2025-4-1 11:30
Flow Cytometric Detection of G0 in Live Cells by Hoechst 33342 and Pyronin Y Staining,ve the same amount of DNA as cells at G1 phase but lower RNA levels compared to proliferating cells. Therefore, resting cells in G0 phase can be distinguished from proliferating cells in G1, S, and G2?M phases. This chapter describes a protocol for double staining of live cells with Hoechst 33342 an作者: Infuriate 時間: 2025-4-1 16:38 作者: ANTIC 時間: 2025-4-1 18:38
Isolation of Neural Stem and Progenitor Cells from the Adult Brain and Live Imaging of Their Cell Conal precursors in the adult mammalian brain. Various approaches of fluorescent-activated cell sorting (FACS) have emerged to allow the distinction between quiescent NSCs (qNSCs), their activated counterpart (aNSCs), and the resulting progeny. In this article, we review two FACS techniques that can
