標題: Titlebook: Cells and Culture; Proceedings of the 2 Thomas Noll Conference proceedings 2010 Springer Science+Business Media B.V. 2010 Proteomics.bioinf [打印本頁] 作者: Strategy 時間: 2025-3-21 16:06
書目名稱Cells and Culture影響因子(影響力)
書目名稱Cells and Culture影響因子(影響力)學(xué)科排名
書目名稱Cells and Culture網(wǎng)絡(luò)公開度
書目名稱Cells and Culture網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Cells and Culture被引頻次
書目名稱Cells and Culture被引頻次學(xué)科排名
書目名稱Cells and Culture年度引用
書目名稱Cells and Culture年度引用學(xué)科排名
書目名稱Cells and Culture讀者反饋
書目名稱Cells and Culture讀者反饋學(xué)科排名
作者: 肌肉 時間: 2025-3-21 20:17 作者: nostrum 時間: 2025-3-22 01:03
Targeted Gene Knockdown Effects on Recombinant Protein Secretion in CHO Cellss of the LEAP instrument to quantify recombinant protein secretion from individual cells. The same siRNAs were co-transfected into parental CHO cells along with the IgG light and heavy chain genes to confirm the results using a transient expression system. These experiments further our understanding作者: Isometric 時間: 2025-3-22 08:17
Towards the Use of CHO Produced Recombinant Extracellular Matrix Proteins as Bioactive Elements in ample fluorescence measurement. Subsequently, we cloned genes encoding two main ECM proteins (collagen I and tropoelastin) into mammalian expression vectors. We showed that recombinant elastin and collagen are both expressed and secreted by CHO DG44 cells. In a next step, CHO cells genetically modifi作者: insightful 時間: 2025-3-22 09:11 作者: 健談的人 時間: 2025-3-22 16:37
Cell Xpress? Technology Facilitates High-Producing Chinese Hamster Ovary Cell Line Generation Using exhibited different degrees of secretion heterogeneity. In conclusion, Cell Xpress? enables high-throughput multi-parameter clone analysis and selection based on IgG secretion intensity and heterogeneity. Single-cell clones may develop secretion heterogeneity during expansion, which may contribute t作者: 健談的人 時間: 2025-3-22 17:50 作者: 鑒賞家 時間: 2025-3-22 23:26
Gene Modified Hematopoietic Stem Cells for the Treatment of Primary Immunodeficiency Diseasese protein products of PID genes are highly expressed in the hematopoietic system, addition of correct copies of the gene to hematopoietic stem cells (HSC) can be expected to restore immune function. Following recent advances in the technology of gene transfer three PID, X-linked severe combined immu作者: 極微小 時間: 2025-3-23 05:19 作者: 圓錐體 時間: 2025-3-23 05:44
https://doi.org/10.1007/978-3-319-73392-0s of the LEAP instrument to quantify recombinant protein secretion from individual cells. The same siRNAs were co-transfected into parental CHO cells along with the IgG light and heavy chain genes to confirm the results using a transient expression system. These experiments further our understanding作者: Essential 時間: 2025-3-23 11:32
Madhura P. Nijsure,Vipuil Kishoremple fluorescence measurement. Subsequently, we cloned genes encoding two main ECM proteins (collagen I and tropoelastin) into mammalian expression vectors. We showed that recombinant elastin and collagen are both expressed and secreted by CHO DG44 cells. In a next step, CHO cells genetically modifi作者: Angiogenesis 時間: 2025-3-23 16:23
https://doi.org/10.1007/978-3-319-73664-8f hMSC at higher population doubling numbers may be of interest due to the requirement of less frequently isolations of cells. Therefore hMSC were cultured in monolayer until the 37th population doubling. Cells of different passages were cultured as pellets for two weeks in transforming growth facto作者: Intact 時間: 2025-3-23 20:32 作者: 土產(chǎn) 時間: 2025-3-24 00:13 作者: 無瑕疵 時間: 2025-3-24 05:23 作者: 潰爛 時間: 2025-3-24 06:34 作者: 易彎曲 時間: 2025-3-24 13:35 作者: 遵循的規(guī)范 時間: 2025-3-24 16:10
al cell technology for industrialists..Practical informationRegeneration of tissue to replace damaged or injured tissue is the goal of t- sue engineering. Biomaterials like polyglycolic acid, collagen and small-intestinal submuscosa provide a temporary scaffold to guide new tissue growth and or- niz作者: aggressor 時間: 2025-3-24 22:02 作者: intricacy 時間: 2025-3-25 03:11
Towards Grounded Enterprise Modellingractivity mainly due to substantial time requirements. However, multiple copies are not limited to gene amplification. Transfection techniques such as calcium phosphate co-precipitation, PEI transfection and even electroporation result in multicopy integrates without the stepwise increase of selection pressure that induce amplification.作者: jabber 時間: 2025-3-25 04:45
https://doi.org/10.1007/978-3-319-73814-7y during this period and remained highly glycosylated contrary to batch processes. The same phenomenon was observed when CHO cells were cultivated in batch process in RPMI medium but supplemented with specific components of the PF BDM medium.作者: brassy 時間: 2025-3-25 11:14 作者: 解凍 時間: 2025-3-25 13:24 作者: 分離 時間: 2025-3-25 17:26 作者: 豐滿中國 時間: 2025-3-25 20:33 作者: 勤勉 時間: 2025-3-26 03:13 作者: OVER 時間: 2025-3-26 05:23
https://doi.org/10.1007/978-3-319-73814-7tric measurement of cell metabolism (Wolf et al., 1998). Extracellular acidification and oxygen consumption by the cells can be directly monitored over several days (Brischwein et al., 2003). The basis of this system is a small sensor chip made of low cost materials combined with highly integrated and sensitive measurement electronics.作者: dagger 時間: 2025-3-26 12:04
Transient Gene Expression in Chinese Hamster Ovary Cells at Low Temperature – The Effects of Cold-Inent. Cold shock proteins are also known to act in a post-transcriptional manner, but the overexpression of the cold-induced RNA binding protein (CIRBP) and the RNA binding motif 3 (Rbm3) had no effect on the enhancement of transient recombinant protein production in CHO cells.作者: 拔出 時間: 2025-3-26 15:43
An Avian Cell Line for Production of Highly Attenuated Viral Vectorsa currently maximum burst size exceeding 16000), the sensitivity of AGE1 CR.pIX to inducers of the antiviral response is elevated, the infectious cycle is not accelerated and cellular proliferation rate is decreased.作者: 晚來的提名 時間: 2025-3-26 18:04 作者: transplantation 時間: 2025-3-26 22:34
https://doi.org/10.1007/978-3-319-73664-8s, thereby allowing the virus to reach higher titers..We employed viral RSSs for improving the productivity of eukaryotic production systems. We here describe the application of viral RSSs in enhancing the production of pharmaceutical proteins including monoclonal antibodies and virus particles in mammalian cell lines.作者: 發(fā)微光 時間: 2025-3-27 02:06
Peptides as Orthopedic Biomaterialsom the highest 25% of secretors based on Cell Xpress? data. These populations were analyzed via transcriptional profiling, copy number analysis, and epigenetic characterization to identify defining properties of cells that maintain or lose short-term production stability.作者: 做作 時間: 2025-3-27 06:43
RNA Silencing Suppressors Boost the Production of Recombinant Proteins and Virusess, thereby allowing the virus to reach higher titers..We employed viral RSSs for improving the productivity of eukaryotic production systems. We here describe the application of viral RSSs in enhancing the production of pharmaceutical proteins including monoclonal antibodies and virus particles in mammalian cell lines.作者: 進取心 時間: 2025-3-27 11:03 作者: 壓艙物 時間: 2025-3-27 16:47
Mahdi Zouch,Wided Guedria,Riadh Ben Halimasecretion of recombinant human IgG in transiently transfected CHOK1SV, ECACC K1 and CHO DG44 parental cell lines. By analyzing these production trends in a transient transfection system, we were able to compare recombinant protein production and secretion between the different CHO parental cell lines independent of integration site effect.作者: 富足女人 時間: 2025-3-27 17:59
https://doi.org/10.1007/978-90-481-3419-9Proteomics; bioinformatics; biotechnology; cell; cell culture; glycoprotein; microarray; physiology; protein作者: SEEK 時間: 2025-3-27 23:06 作者: 柳樹；枯黃 時間: 2025-3-28 04:27 作者: 軟膏 時間: 2025-3-28 07:57
ESACT Proceedingshttp://image.papertrans.cn/c/image/222961.jpg作者: Electrolysis 時間: 2025-3-28 11:14
An Evaluation of the Intrinsic IgG Production Capabilities of Different Chinese Hamster Ovary Parentsecretion of recombinant human IgG in transiently transfected CHOK1SV, ECACC K1 and CHO DG44 parental cell lines. By analyzing these production trends in a transient transfection system, we were able to compare recombinant protein production and secretion between the different CHO parental cell lines independent of integration site effect.作者: JOG 時間: 2025-3-28 15:11
https://doi.org/10.1007/978-3-319-73392-0etter understand the molecular characteristics of high-producing CHO clones, we developed a high throughput assay using RNAi and the Cell Xpress? technology driven by LEAP (Laser Enabled Analysis and Processing) to evaluate the effects of targeted gene knockdown on therapeutic protein expression. Sh作者: alliance 時間: 2025-3-28 22:29 作者: 團結(jié) 時間: 2025-3-29 00:48 作者: 鬧劇 時間: 2025-3-29 05:56 作者: 消息靈通 時間: 2025-3-29 08:06
Peptides as Orthopedic Biomaterialss occurring during influenza A vaccine production in Madin-Darby canine kidney microcarrier culture. Flow cytometry was employed for single-cell based analysis of infection, apoptosis and intracellular accumulation of viral nucleoprotein. Apoptotic DNA strand breaks were observed especially during l作者: PANT 時間: 2025-3-29 14:10
https://doi.org/10.1007/978-3-319-73664-8tial cell source in cartilage tissue engineering. Because of the requirement of high cell amounts for the generation of cartilage implants or for the extensive experimental studies to investigate the culture parameters the initial cells have to be expanded which leads to high population doubling num作者: terazosin 時間: 2025-3-29 18:06
Peptides as Orthopedic Biomaterialsy investigates the molecular basis for the onset of clonal heterogeneity in different recombinant protein (h-IgG) secreting CHO clones by characterizing specific mechanisms of transgene silencing, such as genetic drift, epigenetic modifications, and biochemical pathway perturbations. Using the Cell 作者: conjunctiva 時間: 2025-3-29 22:19 作者: 斷言 時間: 2025-3-30 01:44 作者: DIKE 時間: 2025-3-30 04:09 作者: 繁榮地區(qū) 時間: 2025-3-30 09:00
Towards Grounded Enterprise Modellingof expression units. This simple logic was the rationale for gene amplification strategies that have been applied extensively in the past but lost attractivity mainly due to substantial time requirements. However, multiple copies are not limited to gene amplification. Transfection techniques such as作者: sterilization 時間: 2025-3-30 15:01
https://doi.org/10.1007/978-3-319-73814-7n be rescued with allogeneic stem cell transplantation (SCT). This has resulted in improvement of patient survival, primarily when using HLA identical sibling or unrelated donors. In the absence of HLA identical donors patients can receive an HLA haploidentical SCT from a parent, provided donor stem作者: 使無效 時間: 2025-3-30 19:05 作者: obsolete 時間: 2025-3-31 00:20 作者: 可忽略 時間: 2025-3-31 04:35 作者: 暴露他抗議 時間: 2025-3-31 09:07 作者: Scintillations 時間: 2025-3-31 11:26
Lecture Notes in Computer SciencePharmaceutical and biotechnology industries are experiencing increasing pressures with new drug development timelines. As well as securing a “first to market” commercial advantage, the ability to make rapid decisions during the development cycle of new molecules is crucial.作者: Glutinous 時間: 2025-3-31 13:33
Single Use Bioreactors: Expressing Protein in Mammalian Cell SuspensionPharmaceutical and biotechnology industries are experiencing increasing pressures with new drug development timelines. As well as securing a “first to market” commercial advantage, the ability to make rapid decisions during the development cycle of new molecules is crucial.
