標題: Titlebook: Cell-Free Translation Systems; Alexander S. Spirin Book 2002 Springer-Verlag Berlin Heidelberg 2002 Expression.Polymerasekettenreaktion.Pr [打印本頁] 作者: antihistamine 時間: 2025-3-21 16:22
書目名稱Cell-Free Translation Systems影響因子(影響力)
書目名稱Cell-Free Translation Systems影響因子(影響力)學科排名
書目名稱Cell-Free Translation Systems網(wǎng)絡公開度
書目名稱Cell-Free Translation Systems網(wǎng)絡公開度學科排名
書目名稱Cell-Free Translation Systems被引頻次
書目名稱Cell-Free Translation Systems被引頻次學科排名
書目名稱Cell-Free Translation Systems年度引用
書目名稱Cell-Free Translation Systems年度引用學科排名
書目名稱Cell-Free Translation Systems讀者反饋
書目名稱Cell-Free Translation Systems讀者反饋學科排名
作者: 聯(lián)合 時間: 2025-3-22 00:04 作者: FLASK 時間: 2025-3-22 01:01 作者: 匍匐前進 時間: 2025-3-22 05:40 作者: 極大的痛苦 時間: 2025-3-22 09:01 作者: 無意 時間: 2025-3-22 13:20
Yeast Cell-Free Translation Systemsare easy to handle and amenable to very powerful genetic and biochemical analysis, the yeast system represents an attractive model system for studies on the mechanism and regulation of eukaryotic translation. The first cell-free systems capable of initiating translation on exogenous mRNA were prepar作者: 無意 時間: 2025-3-22 17:16
Poly(A)-Dependent Cell-Free Translation Systems from Animal Cellscial class of RNA molecule: messenger RNA. Extracts derived from rabbit reticulocytes, Krebs II ascites cells and HeLa cells were instrumental in the identification of messenger RNA as the ultimate carrier of information to the protein synthesis machinery (Labrie 1969). Subsequently, in vitro system作者: lavish 時間: 2025-3-23 01:04
Continuous-Flow and Continuous-Exchange Cell-Free Translation Systems and Reactors expression of foreign genes in bacterial or eukaryotic cells are efficient and widely used. However, difficulties associated with cytotoxicity, proteolytic degradation or improper folding and aggregation of synthesized proteins are often encountered .. The . expression systems are restricted by mec作者: 教義 時間: 2025-3-23 02:50 作者: 口味 時間: 2025-3-23 09:25 作者: 無能力 時間: 2025-3-23 11:31
Expression of an Aggregation-Prone Protein in the RTS 500 Systeme and aggregated forms or inclusion bodies. The conventional molecular mechanism of this process suggests that nascent polypeptide chains have two alternative and competitive pathways between folding and aggregation (King et al. 1996). Although solubilization/refolding procedures exist to obtain pro作者: 紳士 時間: 2025-3-23 14:46 作者: 手段 時間: 2025-3-23 21:24 作者: 拋棄的貨物 時間: 2025-3-24 00:40 作者: defray 時間: 2025-3-24 06:22 作者: 史前 時間: 2025-3-24 09:25 作者: miscreant 時間: 2025-3-24 13:07 作者: 加入 時間: 2025-3-24 18:36
Planning and Management Concepts,For a long time, cell-free protein synthesis was more or less an analytical method due to low synthesis rate and productivity. However, several key improvements over the last decades have lead to protocols providing real preparative amounts of protein:作者: preeclampsia 時間: 2025-3-24 19:39 作者: Entirety 時間: 2025-3-25 01:21
Miriam Adebibe,Simon W. Coppackd can continue after cell disruption. Thus, disrupted cells or their isolated fractions were reported to be capable of synthesizing proteins (Borsook 1950; Winnick 1950a, 1950b; Siekevitz and Zamecnik 1951; Siekevitz 1952; Peterson and Greenberg 1952; Khesin 1953; Gale and Folkes 1954). In the meant作者: 產(chǎn)生 時間: 2025-3-25 06:14
Simon P. L. Dexter,Mervyn Deiteltrafiltration membranes has some remaining limitations. These can be overcome by introducing an affinity system. A cell-free protein synthesis system has therefore been employed to produce bovine heart fatty acid binding protein (FABP) and bacterial chloramphenicol acetyltransferase (CAT) with and w作者: ADORE 時間: 2025-3-25 10:00
Condylar and Supracondylar Fracture,on and mammalian or insect cell culture. In contrast to the . gene expression methods where protein synthesis is carried out in the context of cell physiology and is surrounded by cell walls and membranes, cell-free protein synthesis provides a completely open system. This allows direct access to th作者: 真繁榮 時間: 2025-3-25 12:17
Symmetric and Isometric Relationsof producing more than 0.1 mg protein in 1 ml reaction within one hour and has increased durability compared with conventional cell-extract in a batch system. The PURE system produced active enzymes without the aid of molecular chaperones. The PURE system that is reinforced with trans-translation sy作者: FILTH 時間: 2025-3-25 18:09 作者: 眨眼 時間: 2025-3-25 22:55
Symmetric and Isometric Relationsare easy to handle and amenable to very powerful genetic and biochemical analysis, the yeast system represents an attractive model system for studies on the mechanism and regulation of eukaryotic translation. The first cell-free systems capable of initiating translation on exogenous mRNA were prepar作者: SENT 時間: 2025-3-26 00:08
Fourier Transforms in Clifford Analysis,cial class of RNA molecule: messenger RNA. Extracts derived from rabbit reticulocytes, Krebs II ascites cells and HeLa cells were instrumental in the identification of messenger RNA as the ultimate carrier of information to the protein synthesis machinery (Labrie 1969). Subsequently, in vitro system作者: 苦笑 時間: 2025-3-26 07:57
Martin F. Kraus,Joachim Hornegger expression of foreign genes in bacterial or eukaryotic cells are efficient and widely used. However, difficulties associated with cytotoxicity, proteolytic degradation or improper folding and aggregation of synthesized proteins are often encountered .. The . expression systems are restricted by mec作者: 擺動 時間: 2025-3-26 09:06 作者: 細微差別 時間: 2025-3-26 13:04
ICU Staffing, Models, and Outcomessis of biologically active products. At the same time, a lot of experimental data indicate that the folding of newly synthesized proteins differs significantly from the . process observed by Anfinsen in his classical experiments on the refolding of ribonuclease (Anfinsen 1973) and studied in detail 作者: 愛社交 時間: 2025-3-26 19:01 作者: libertine 時間: 2025-3-26 23:05
ICU Staffing, Models, and Outcomestein produced as inclusion bodies in . can be expressed as a soluble and active protein in an .-based cell-free expression system, such as the RTS [1]. However, if aggregation also occurs in cell-free systems, the conditions can easily be adjusted to overcome the problem. For example, the addition o作者: 細菌等 時間: 2025-3-27 02:20 作者: 關節(jié)炎 時間: 2025-3-27 07:21 作者: Obstreperous 時間: 2025-3-27 12:53
Improved Batch Translation System Based on , Extract (SFCF) reactor was achieved. The results document that it is possible to avoid the limitations of the ultrafiltration membranes during product removal from a CFCF reactor. The data presented show that the affinity system is also well suited for the development of a novel protein bioreactor.作者: 上腭 時間: 2025-3-27 15:17 作者: 滑稽 時間: 2025-3-27 20:03 作者: 通便 時間: 2025-3-27 22:43
Co-Translational Protein Folding in Prokaryotic and Eukaryotic Cell-Free Translation Systemst peptide starts its folding as soon as it is synthesized and emerges from the ribosome prior to the formation of the entire polypeptide chain (see refs below). The existence of stepwise protein folding starting from the N-terminal section of a growing polypeptide and progressively proceeding toward作者: indoctrinate 時間: 2025-3-28 04:34
Expression of an Aggregation-Prone Protein in the RTS 500 Systemch serves as periplasmic receptor for the high-affinity transport of maltodextrins and for a defective MalE folding mutant, MalE31, that forms inclusion bodies when expressed in bacteria (Betton and Hofnung 1996).作者: 澄清 時間: 2025-3-28 09:10 作者: 誘惑 時間: 2025-3-28 10:42 作者: FEAT 時間: 2025-3-28 14:48 作者: MAIM 時間: 2025-3-28 20:56 作者: 畸形 時間: 2025-3-29 00:24 作者: 厚顏 時間: 2025-3-29 04:14
Miriam Adebibe,Simon W. Coppack also papers in Roberts 1958). The protein-synthesizing ability of these particles was experimentally proved (Littlefield et al. 1955; Littlefield and Keller 1957; McQuillen et al. 1959). The word “ribosome”, was proposed to designate the protein-synthesizing ribonucleoprotein particles.作者: goodwill 時間: 2025-3-29 09:57 作者: 意見一致 時間: 2025-3-29 14:15 作者: 文字 時間: 2025-3-29 17:45
Yeast Cell-Free Translation Systemsor activity through various means, including downregulation of factor synthesis, expression of conditional lethal factor or knock-out mutants and inhibition of factor activity by antibodies. Such systems have been used to extend and deepen our knowledge of the mechanism of translation initiation, protein targeting and translation termination.作者: OMIT 時間: 2025-3-29 20:09 作者: gusher 時間: 2025-3-30 03:06
Symmetric and Isometric Relations system. The PURE system produced active enzymes without the aid of molecular chaperones. The PURE system that is reinforced with trans-translation system shows increased productivity and addition of human placental RNase inhibitor also resulted in prolonged production time.作者: Patrimony 時間: 2025-3-30 06:24
ICU Staffing, Models, and Outcomes. However, if aggregation also occurs in cell-free systems, the conditions can easily be adjusted to overcome the problem. For example, the addition of detergents, cofactors, or molecular chaperones can often enhance or achieve protein solubility.作者: Popcorn 時間: 2025-3-30 10:00
Alberto Alonso-González,Irena Hajnsekomerase also counteracts progressive shortening due to telomeric loss during cellular replication. Cells with inactivated telomerase show a decreased capacity for replication followed by senescence and cell death (Feng et al. 1995).作者: 固定某物 時間: 2025-3-30 14:24 作者: 禁止 時間: 2025-3-30 18:18 作者: 不連貫 時間: 2025-3-31 00:46
Cell-Free Expression of a 127 kDa Protein: The Catalytic Subunit of Human Telomeraseomerase also counteracts progressive shortening due to telomeric loss during cellular replication. Cells with inactivated telomerase show a decreased capacity for replication followed by senescence and cell death (Feng et al. 1995).
