標(biāo)題: Titlebook: Cell‘Cell Interactions; Methods and Protocol Sean P. Colgan Book 2006 Humana Press 2006 bacteria.biology.biosynthesis.cell.cloning.membrane [打印本頁(yè)] 作者: HEM 時(shí)間: 2025-3-21 19:34
書(shū)目名稱(chēng)Cell‘Cell Interactions影響因子(影響力)
作者: placebo-effect 時(shí)間: 2025-3-22 00:09
Analysis of Mammalian Sperm-Egg Membrane Interactions During In Vitro Fertilization,The interactions between egg and sperm are among the most fascinating in cell biology. These interactions include Cell-Cell adhesion and then membrane fusion between the two gametes. This chapter details the experimental methods used to for gamete culture and in vitro fertilization using mouse sperm and eggs.作者: CLASP 時(shí)間: 2025-3-22 01:33 作者: 責(zé)任 時(shí)間: 2025-3-22 06:33 作者: Chronic 時(shí)間: 2025-3-22 12:33
Okinawa and Jeju: Bases of Discontentat brain endothelial cell line 4 (RBE4) express many properties that are expressed by brain endothelial cells in vivo. Tissue culture methods allow the investigator to design experiments for studying transporters and permeability that would be much more difficult in vivo. A method for making prepara作者: 面包屑 時(shí)間: 2025-3-22 13:46
Okinawa and Jeju: Bases of Discontential cells (IECs) represent an important barrier between lamina propria cells and the potentially harmful lumenal contents. In addition, IECs are important immunoeffector cells with the capacity to release cytokines, chemokines, and other molecules involved in antigen presentation and immune defense.作者: 面包屑 時(shí)間: 2025-3-22 20:57 作者: 奴才 時(shí)間: 2025-3-23 00:30 作者: figment 時(shí)間: 2025-3-23 01:25 作者: FID 時(shí)間: 2025-3-23 08:37 作者: Carbon-Monoxide 時(shí)間: 2025-3-23 13:29 作者: aristocracy 時(shí)間: 2025-3-23 14:51 作者: Eructation 時(shí)間: 2025-3-23 21:43 作者: adipose-tissue 時(shí)間: 2025-3-24 00:16 作者: GLUT 時(shí)間: 2025-3-24 04:58
https://doi.org/10.1057/9781137390233hanisms that mediate leukocyte recruitment during an inflammatory response. In this chapter, we present a detailed method for the isolation and in vitro culture of murine vascular endothelial cells from the lung and heart. The endothelial cells are of high purity (85–99%) and retain certain of their作者: BOLT 時(shí)間: 2025-3-24 09:27 作者: Barrister 時(shí)間: 2025-3-24 11:10 作者: 陰險(xiǎn) 時(shí)間: 2025-3-24 18:19
Open Innovation through Strategic Alliancesecrosis factor-α. Increased mucosal levels of these cytokines have been observed in mucosal biopsies from patients with a chronic inflammatory condition referred to as inflammatory bowel disease. Paracellular permeability across epithelial cells is regulated by tight junctions (TJ.), which are the a作者: 混雜人 時(shí)間: 2025-3-24 22:33 作者: BROTH 時(shí)間: 2025-3-25 02:18 作者: Throttle 時(shí)間: 2025-3-25 05:51 作者: Affectation 時(shí)間: 2025-3-25 10:00 作者: 剛開(kāi)始 時(shí)間: 2025-3-25 13:33
Sean P. ColganIncludes supplementary material: 作者: 黃瓜 時(shí)間: 2025-3-25 17:15 作者: 容易生皺紋 時(shí)間: 2025-3-25 20:37
Okinawa and Jeju: Bases of Discontente families of efflux transporters. These in vitro methods will complement many of the in vivo techniques, and they may be used as screening for more timely and expensive experiments, and also reducing the need for experimental animals.作者: 巨頭 時(shí)間: 2025-3-26 00:48 作者: 印第安人 時(shí)間: 2025-3-26 05:24 作者: CHIDE 時(shí)間: 2025-3-26 09:59
Overcoming the Poststructuralist Critique?enzymes, the ecto-apyrase (CD39, metabolizes ATP to AMP) and the 5’-ecto-nucleotidase (CD73, metabolizes AMP to adenosine). Adenosine generated in this fashion can activate endothelial adenosine receptors, leading to increases in intracellular cyclic AMP and resealing of the endothelial junctions, t作者: Anonymous 時(shí)間: 2025-3-26 12:57
Framing Power and the Need to Reverse reside within the gastrointestinal tract. Additionally, the extracellular matrix is composed of a complex infrastructure that includes collagen and other molecules. The manner in which these two components interact is not certain, but the use of recent model systems has provided insights into these作者: Instrumental 時(shí)間: 2025-3-26 20:17 作者: cognizant 時(shí)間: 2025-3-26 23:00
https://doi.org/10.1057/9781137393081n bcl-2. Our strategy described in this chapter uses flow cytometry and a reporter construct in which the bcl-2 promoter is linked to enhanced green fluorescence protein. The advantage of using flow cytometry in expression cloning is that it increases the sensitivity of the screen by enabling us to 作者: Decibel 時(shí)間: 2025-3-27 02:32 作者: 熱烈的歡迎 時(shí)間: 2025-3-27 06:19 作者: 向下五度才偏 時(shí)間: 2025-3-27 13:26 作者: Condescending 時(shí)間: 2025-3-27 15:41 作者: anus928 時(shí)間: 2025-3-27 20:11 作者: Customary 時(shí)間: 2025-3-27 23:31 作者: Between 時(shí)間: 2025-3-28 02:26 作者: amygdala 時(shí)間: 2025-3-28 07:23
Collagen Gel Contraction Assay, reside within the gastrointestinal tract. Additionally, the extracellular matrix is composed of a complex infrastructure that includes collagen and other molecules. The manner in which these two components interact is not certain, but the use of recent model systems has provided insights into these作者: Chipmunk 時(shí)間: 2025-3-28 14:29
,Bacterial–Bacterial Cell Interactions in Biofilms: Detection of Polysaccharide Intercellular Adhesi polymer of .-acetyl-glucosamine. In this chapter, we outline two methods that use wheat germ agglutinin, a lectin that binds to .-acetyl-glucosamine, to evaluate extracellular polysaccharide production by a variety of bacterial species.作者: Resign 時(shí)間: 2025-3-28 18:36 作者: 暗諷 時(shí)間: 2025-3-28 20:01 作者: tinnitus 時(shí)間: 2025-3-29 02:57 作者: enumaerate 時(shí)間: 2025-3-29 06:37
,Cell-Cell Interaction in the Transcellular Biosynthesis of Novel ω-3-Derived Lipid Mediators,erpart generated without aspirin, namely the 17S-RvDs. In this chapter, we provide an overview and detail protocols of the biosynthesis and bioactions of these newly uncovered pathways and products that include three distinct series: 18Rresolvins of the E series derived from EPA (i.e., RvE1); 17R-re作者: fastness 時(shí)間: 2025-3-29 08:29 作者: generic 時(shí)間: 2025-3-29 13:03 作者: 召集 時(shí)間: 2025-3-29 18:13
The Role of Junctional Adhesion Molecules in Interactions Between Vascular Cells, are regulated by adhesion receptors and can be homotypic, that is, between cells of the same type, for example, between adjacent endothelial cells in the vasculature, as well as heterotypic, that is, between different cells, such as the leukocyte endothelial interactions that take place during leuk作者: 中止 時(shí)間: 2025-3-29 20:09 作者: Nucleate 時(shí)間: 2025-3-30 01:46 作者: 激怒某人 時(shí)間: 2025-3-30 07:40
Nucleotide Metabolism and Cell-Cell Interactions,l–endothelial crosstalk pathways that modulate inflammatory responses and vascular barrier function. For example, during episodes of inflammation, the transendothelial migration (TEM) of PMNs potentially disturbs vascular barrier and gives rise to intravascular fluid extravasation and edema. However作者: 取回 時(shí)間: 2025-3-30 10:22
Collagen Gel Contraction Assay,hmic pattern of contractions controlled by its intrinsic neuronal innvervation. In chronic inflammatory diseases such as Crohn’s disease, the intestine can become stiff and fibrotic, losing much of its normal motility. Although muscle fiber contraction accounts for much of this activity, contraction作者: 值得 時(shí)間: 2025-3-30 14:01 作者: 譏諷 時(shí)間: 2025-3-30 17:00
,Bacterial–Bacterial Cell Interactions in Biofilms: Detection of Polysaccharide Intercellular Adhesibiofilm formation in the pathogenesis of certain types of infection, especially those involving indwelling medical devices, is becoming increasingly apparent. Critical to the development of a biofilm is the elaboration of exopolysaccharide that contributes to substrate and intercellular adhesion. Th作者: Acquired 時(shí)間: 2025-3-30 20:59
A Biochemical Method for Tracking Cholera Toxin Transport From Plasma Membrane to Golgi and Endoplaenterotoxin produced by the bacterium .. The toxin must enter the intestinal cell to cause disease. Entry is achieved by the B-subunit binding to a membrane lipid that carries the toxin all the way from the plasma mem-brane through the .-Golgi to the endoplasmic reticulum (ER). Once in the ER, a por作者: gregarious 時(shí)間: 2025-3-31 04:56 作者: palliative-care 時(shí)間: 2025-3-31 06:10
Expression Cloning of Signaling Proteins Regulated by Cell Adhesion,ation. Adhesion-related signal transduction is important throughout development, hemostasis, immunity, and in diseases such as cancer. Therefore, the identification of the various signaling pathways that are involved is crucial. Expression cloning is an unbiased way to isolate proteins with specific作者: tattle 時(shí)間: 2025-3-31 11:52
Assays for the Calcium Sensitivity of Desmosomes,ogenesis, or if they are taking part in invasion and metastasis. Desmosomes are one of the principal types of adhesive junctions in epithelia and are responsible for maintaining tissue integrity. It is likely that modulation of desmosomal adhesion is required to facilitate cell motility in response 作者: 誹謗 時(shí)間: 2025-3-31 14:03
,Tight Junctions and Cell–Cell Interactions,ecrosis factor-α. Increased mucosal levels of these cytokines have been observed in mucosal biopsies from patients with a chronic inflammatory condition referred to as inflammatory bowel disease. Paracellular permeability across epithelial cells is regulated by tight junctions (TJ.), which are the a作者: 某人 時(shí)間: 2025-3-31 18:56 作者: 喚醒 時(shí)間: 2025-3-31 23:02 作者: 沙漠 時(shí)間: 2025-4-1 04:28
The Blot Rolling Assay: A Method for Identifying Adhesion Molecules Mediating Binding Under Shear Crafficking, tumor metastasis, and atherosclerosis. We have developed a new technique for the observation of binding interactions under shear, which we have termed the “blot rolling assay.” In this method, molecules in a complex mixture are resolved by gel electrophoresis and transferred to a membran作者: 官僚統(tǒng)治 時(shí)間: 2025-4-1 07:48
