標(biāo)題: Titlebook: Cell Viability Assays; Methods and Protocol Oliver Friedrich,Daniel F. Gilbert Book 2023Latest edition The Editor(s) (if applicable) and Th [打印本頁(yè)] 作者: Reticent 時(shí)間: 2025-3-21 19:11
書(shū)目名稱Cell Viability Assays影響因子(影響力)
作者: 擴(kuò)張 時(shí)間: 2025-3-21 21:45 作者: 方便 時(shí)間: 2025-3-22 01:59
Assaying Proliferation Characteristics of Cells Cultured Under Static Versus Periodic Conditions Common in vitro culture models are typically maintained under static conditions, where the surrounding culture medium is replaced every few days—typically every 48 to 72?h—with the aim to remove metabolites and to replenish nutrients. Although this approach is sufficient for supporting cellular sur作者: intertwine 時(shí)間: 2025-3-22 04:41 作者: Preserve 時(shí)間: 2025-3-22 12:46
Assaying Mitochondrial Function by Multiparametric Flow Cytometryhemical attributes of individual cells within a larger population. More recently, advances in flow cytometry have enabled nanoparticle detection. This is particularly applicable to mitochondria, which, as intracellular organelles have distinct subpopulations that can be evaluated based on difference作者: CEDE 時(shí)間: 2025-3-22 13:17 作者: CEDE 時(shí)間: 2025-3-22 17:12 作者: encomiast 時(shí)間: 2025-3-22 23:08
Average Rheological Quantities of Cells in Monolayersgether in a single layer. Here we describe step-by-step procedure as to how to employ a modified commercial rotational rheometer to run rheological measurement and detect average viscoelastic properties of cells while maintaining the necessary precision level at the same time.作者: echnic 時(shí)間: 2025-3-23 04:18
Assaying Spontaneous Network Activity and Cellular Viability Using Multi-Well Microelectrode Arraysmine chemical effects thereon. Following assessment of compound effects on multiple endpoints that evaluate network function, a cell viability endpoint in the same well is determined using a multiplexed approach. Recently, it has become possible to measure electrical impedance of cells attached to t作者: GLADE 時(shí)間: 2025-3-23 08:44 作者: parasite 時(shí)間: 2025-3-23 09:49
Assessment of Cell Viability in Electrically Excitable Muscle Cells Through Intact Twitch Stimulationvolves intact membrane integrity with polarized membrane, functional ion channels for action potential generation and conduction, an intact electro-chemical interface at the level of the fiber’s triad, followed by sarcoplasmic reticulum Ca. release, and subsequent activation of the chemico-mechanic作者: Incorruptible 時(shí)間: 2025-3-23 17:02
Evaluating Cellular Viability by iTRAQ Proteomic Profilingproteomes and metabolomes. These “omic” profiles are in constant flux even during normal cellular functioning, to maintain cellular homeostasis, in response to small environmental changes and maintenance of optimal cell viability. However proteomic “fingerprints” can also provide insight into cellul作者: muster 時(shí)間: 2025-3-23 22:06
Quantifying pH in Malaria Using pHluorin and Flow Cytometryation within cells. Slight pH changes can impact the regulation of diverse molecular processes, including enzymatic activities, ion channels, and transporters, which all play a role in cell functions. Methods for quantifying pH. continue to evolve and include various optical methods using fluorescen作者: HEDGE 時(shí)間: 2025-3-23 22:13
Cell Membrane State, Permeability, and Elasticity Assessment for Single Cells and Cell Ensemblesrmeability and bending modulus. Although the main transition of lipid membranes is typically detected employing differential scanning calorimetry (DSC), this technique is not suitable for many biological membranes. Moreover, often single cell data on the membrane state or order is of interest. We he作者: Glaci冰 時(shí)間: 2025-3-24 04:58
Neutral Red Uptake Assay to Assess Cytotoxicity In Vitroability of living cells to incorporate neutral red, a weak cationic dye, in lysosomes. The quantification of xenobiotic-induced cytotoxicity is expressed as a concentration-dependent reduction of the uptake of neutral red when compared to cells exposed to corresponding vehicle controls. The NRU assa作者: 放棄 時(shí)間: 2025-3-24 09:26 作者: misanthrope 時(shí)間: 2025-3-24 10:48 作者: colostrum 時(shí)間: 2025-3-24 16:09 作者: staging 時(shí)間: 2025-3-24 21:53
Cell Viability Assays978-1-0716-3052-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: APEX 時(shí)間: 2025-3-24 23:23
Colin J. Bushnell,Irving Reinergether in a single layer. Here we describe step-by-step procedure as to how to employ a modified commercial rotational rheometer to run rheological measurement and detect average viscoelastic properties of cells while maintaining the necessary precision level at the same time.作者: FOLLY 時(shí)間: 2025-3-25 04:10 作者: VOC 時(shí)間: 2025-3-25 08:35 作者: Subjugate 時(shí)間: 2025-3-25 13:31 作者: Finasteride 時(shí)間: 2025-3-25 19:47 作者: 低能兒 時(shí)間: 2025-3-25 20:03 作者: ligature 時(shí)間: 2025-3-26 02:40 作者: certitude 時(shí)間: 2025-3-26 07:46
Inverse methods for reflector imaging,hemical attributes of individual cells within a larger population. More recently, advances in flow cytometry have enabled nanoparticle detection. This is particularly applicable to mitochondria, which, as intracellular organelles have distinct subpopulations that can be evaluated based on difference作者: Thrombolysis 時(shí)間: 2025-3-26 10:56 作者: corporate 時(shí)間: 2025-3-26 15:44 作者: Nonconformist 時(shí)間: 2025-3-26 19:33 作者: Nomogram 時(shí)間: 2025-3-26 21:39 作者: 乞丐 時(shí)間: 2025-3-27 01:13
https://doi.org/10.1007/BFb0074922 and responding to mechanical forces and pathophysiological variations in these processes have become an emerging research field in recent years. An important signaling molecule involved in mechanotransduction as in many cellular processes is Ca.. New experimental methods to probe cellular Ca. signa作者: Anticonvulsants 時(shí)間: 2025-3-27 08:16 作者: APO 時(shí)間: 2025-3-27 12:54 作者: 迷住 時(shí)間: 2025-3-27 16:41 作者: Insulin 時(shí)間: 2025-3-27 18:24
Optimization and Related Fieldsrmeability and bending modulus. Although the main transition of lipid membranes is typically detected employing differential scanning calorimetry (DSC), this technique is not suitable for many biological membranes. Moreover, often single cell data on the membrane state or order is of interest. We he作者: hereditary 時(shí)間: 2025-3-27 22:44 作者: 儀式 時(shí)間: 2025-3-28 02:47 作者: Gudgeon 時(shí)間: 2025-3-28 09:31 作者: 翅膀拍動(dòng) 時(shí)間: 2025-3-28 10:26 作者: Asperity 時(shí)間: 2025-3-28 17:52
Oliver Friedrich,Daniel F. GilbertIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts作者: linear 時(shí)間: 2025-3-28 22:20
Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/222896.jpg作者: 使習(xí)慣于 時(shí)間: 2025-3-29 00:55
Average Rheological Quantities of Cells in Monolayersgether in a single layer. Here we describe step-by-step procedure as to how to employ a modified commercial rotational rheometer to run rheological measurement and detect average viscoelastic properties of cells while maintaining the necessary precision level at the same time.作者: 彎彎曲曲 時(shí)間: 2025-3-29 04:30
Assaying Proliferation Characteristics of Cells Cultured Under Static Versus Periodic Conditionsf cellular growth under static versus pulsed-perfused conditions, mimicking continuous replacement of extracellular fluid in the physiological environment. The protocol involves long-term life-cell high-content time-lapse imaging of fluorescent cells at 37?°C and ambient CO. concentration using mult作者: braggadocio 時(shí)間: 2025-3-29 09:39
Network Reconstruction as a Novel High-Level Marker of Functional Neuronal Viabilityas the modularity, the centrality, or the characteristic path length. In summary, these parameters describe the network and how it is influenced by experimental modulations, for example, hypoxia, nutrient deficiency, co-culture models, or application of drugs and other factors.作者: 激怒 時(shí)間: 2025-3-29 15:15 作者: CHOP 時(shí)間: 2025-3-29 19:10 作者: 為現(xiàn)場(chǎng) 時(shí)間: 2025-3-29 22:15
Assessment of Cell Viability in Electrically Excitable Muscle Cells Through Intact Twitch Stimulatio this chapter, we describe step-by-step protocols to (i) obtain intact single muscle fibers from freshly dissected muscle tissue using an enzymatic digestion procedure and (ii) provide a workflow for the assessment of twitch response of single fibers that can be ultimately classified as viable. For 作者: LATHE 時(shí)間: 2025-3-30 00:49
Ordinary and Partial Differential Equationsf cellular growth under static versus pulsed-perfused conditions, mimicking continuous replacement of extracellular fluid in the physiological environment. The protocol involves long-term life-cell high-content time-lapse imaging of fluorescent cells at 37?°C and ambient CO. concentration using mult作者: 發(fā)炎 時(shí)間: 2025-3-30 07:35 作者: 鴿子 時(shí)間: 2025-3-30 09:30 作者: 離開(kāi) 時(shí)間: 2025-3-30 15:41
https://doi.org/10.1007/BFb0074922 show a protocol for functional screening of mechanosensitive ion channels and related drug screenings using BJ cells, a foreskin fibroblast cell line that strongly reacts to acute mechanical stimulation.作者: 不近人情 時(shí)間: 2025-3-30 19:53 作者: 喧鬧 時(shí)間: 2025-3-30 20:41 作者: 無(wú)孔 時(shí)間: 2025-3-31 01:29
Book 2023Latest edition and up-to-date, .Cell Viability Assays: Methods and Protocols, Second Edition. serves as a valuable resource to the growing community in bioinspired life sciences, biomedical sciences, and biotechnology by providing more standardized protocols to probe the “wellbeing” of cells in various environmen作者: 庇護(hù) 時(shí)間: 2025-3-31 05:26 作者: excrete 時(shí)間: 2025-3-31 10:35
Optimization and Related Fieldss can be used to determine qualitative and quantitative proteomic change. In this chapter, we will focus on a labeling method called isobaric tags for relative and absolute quantification (iTRAQ), which is frequently used to identify and quantify proteomic expression changes in cells and tissues.作者: 四目在模仿 時(shí)間: 2025-3-31 13:54
A scenario for local properness,s and step-by-step protocols to guide the processes of obtaining SHG microscopy image data from samples, as well as extracting characteristic values from the image data to quantify the cellular microarchitecture using characteristic patterns of myofibrillar lattice alignments.作者: pancreas 時(shí)間: 2025-3-31 21:31 作者: Talkative 時(shí)間: 2025-4-1 00:27 作者: 條街道往前推 時(shí)間: 2025-4-1 05:08 作者: dithiolethione 時(shí)間: 2025-4-1 09:44 作者: 監(jiān)禁 時(shí)間: 2025-4-1 12:55
