標(biāo)題: Titlebook: Cell Penetrating Peptides; Methods and Protocol ülo Langel Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under e [打印本頁] 作者: 小巷 時(shí)間: 2025-3-21 16:04
書目名稱Cell Penetrating Peptides影響因子(影響力)
作者: 枯萎將要 時(shí)間: 2025-3-22 00:16
Optimization of Elliptic Systemsg (SAXS) as a suitable method for unveiling their nanoscale organization. A roadmap for standard SAXS measurements in CPP/DNA samples is presented alongside a selection of observations from our own experience dealing with SAXS applied to the investigation of CPPs.作者: Extort 時(shí)間: 2025-3-22 03:06
Methods for Small-Angle Scattering Measurements on Peptiplexes of DNA with Cell-Penetrating Peptidesg (SAXS) as a suitable method for unveiling their nanoscale organization. A roadmap for standard SAXS measurements in CPP/DNA samples is presented alongside a selection of observations from our own experience dealing with SAXS applied to the investigation of CPPs.作者: irradicable 時(shí)間: 2025-3-22 07:23
Modelling of Sewer Network Flow,section some features of the thermodynamics of CPP interaction with the membrane is also presented. Finally, we present different cargoes that can be transferred into the cells by CPPs and briefly discuss the effect of cargo on the rate and efficiency of penetration into the cells.作者: Neutral-Spine 時(shí)間: 2025-3-22 10:50 作者: optional 時(shí)間: 2025-3-22 15:14 作者: optional 時(shí)間: 2025-3-22 17:08 作者: anatomical 時(shí)間: 2025-3-22 21:48 作者: 轉(zhuǎn)換 時(shí)間: 2025-3-23 03:14
Optimization of Elliptic Systems generation and purification of eGFP attached to the cyclic form of the newly discovered CPP EJP18 in . We also demonstrate a novel microscopy method for quantifying its subcellular distribution in leukemia cells.作者: 粗鄙的人 時(shí)間: 2025-3-23 06:12
K. Fekete,J. Manninger,P. Cserháti a wide variety of proteins, including enzymes, transcription factors, tumor suppressor proteins, and many more. TAT-fusion proteins are generated cloning in-frame into the pTAT-HA plasmid, then transformed into . for expression, and purified by the 6-His affinity tag over Ni-NTA column, followed by a final IEX FPLC purification step.作者: BROTH 時(shí)間: 2025-3-23 12:29
Synthesis of Cell-Penetrating Peptide Coated Silica Nanoparticles and Their Physicochemical and Biol-known inorganic drug delivery vehicle type, and their surface modification with cell-penetrating peptides using sC18 and derivatives thereof. Moreover, physicochemical as well as biological characterization methods, including cellular uptake measurements, of particle-peptide conjugates are described.作者: glisten 時(shí)間: 2025-3-23 17:48 作者: INCUR 時(shí)間: 2025-3-23 19:13
Quantitative Subcellular Analysis of Cyclic Cell-Penetrating Peptide EJP18 in Nonadherent Cells generation and purification of eGFP attached to the cyclic form of the newly discovered CPP EJP18 in . We also demonstrate a novel microscopy method for quantifying its subcellular distribution in leukemia cells.作者: 別炫耀 時(shí)間: 2025-3-24 00:05
Protein Delivery by PTDs/CPPs a wide variety of proteins, including enzymes, transcription factors, tumor suppressor proteins, and many more. TAT-fusion proteins are generated cloning in-frame into the pTAT-HA plasmid, then transformed into . for expression, and purified by the 6-His affinity tag over Ni-NTA column, followed by a final IEX FPLC purification step.作者: 釘牢 時(shí)間: 2025-3-24 03:56
Magdalene Marinaki,Markos Papageorgiouand animals, it is likely that this signaling activity appeared before the separation between plants, fungi, and animals, and is therefore very ancient. These aspects are discussed in the present review, with an accent placed on evolution and on the comparison of homeoprotein signaling between species belonging to distinct phyla.作者: 尾隨 時(shí)間: 2025-3-24 09:46
Magdalene Marinaki,Markos Papageorgiousk can be achieved in a few rounds of breeding. The procedure is simple and straightforward on the synthetic site but requires robust, highly reproducible and close-to-reality biological assays to yield realistic functional output. With this technology even top-performing peptides can be further improved and functionally adjusted.作者: 小教堂 時(shí)間: 2025-3-24 14:27 作者: abject 時(shí)間: 2025-3-24 15:25
Pekka Neittaanmaki,Dan Tiba,Jürgen Sprekelsmapping of trafficking routes. In this chapter, we describe protocols for a highly defined formation of polyplexes. We then point out the use of fluorescent fusion proteins to identify subcellular trafficking compartments and image analysis protocols to obtain quantitative information on trafficking routes and endosomal release.作者: FLAT 時(shí)間: 2025-3-24 19:57
Shuttling Homeoproteins and Their Biological Significanceand animals, it is likely that this signaling activity appeared before the separation between plants, fungi, and animals, and is therefore very ancient. These aspects are discussed in the present review, with an accent placed on evolution and on the comparison of homeoprotein signaling between species belonging to distinct phyla.作者: 發(fā)出眩目光芒 時(shí)間: 2025-3-25 03:08
Artificial Evolutionary Optimization Process to Improve the Functionality of Cell Penetrating Peptidsk can be achieved in a few rounds of breeding. The procedure is simple and straightforward on the synthetic site but requires robust, highly reproducible and close-to-reality biological assays to yield realistic functional output. With this technology even top-performing peptides can be further improved and functionally adjusted.作者: N斯巴達(dá)人 時(shí)間: 2025-3-25 04:08
In Silico Design of Chemically Modified Cell-Penetrating Peptidesen developed by our group to predict cell-penetration potential of chemically modified peptides. This chapter is dedicated for designing therapeutically important cell-penetrating peptides using CellPPDMod (.).作者: Munificent 時(shí)間: 2025-3-25 11:03 作者: Carcinogen 時(shí)間: 2025-3-25 13:54 作者: 價(jià)值在貶值 時(shí)間: 2025-3-25 15:48
Cell-Penetrating Peptidessection some features of the thermodynamics of CPP interaction with the membrane is also presented. Finally, we present different cargoes that can be transferred into the cells by CPPs and briefly discuss the effect of cargo on the rate and efficiency of penetration into the cells.作者: 詞匯記憶方法 時(shí)間: 2025-3-25 19:58
Synthetic Molecular Evolution of Cell Penetrating Peptidesis of iterative, knowledge-based peptide libraries and the screening of such libraries in complex orthogonal cell-based screens for improved activity. In this chapter, we describe methods for synthesizing powerful combinatorial peptide libraries for synthetic molecular evolution.作者: Agility 時(shí)間: 2025-3-26 02:39
ülo LangelIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible result.Contains key notes and implementation advice from the experts?作者: 事先無準(zhǔn)備 時(shí)間: 2025-3-26 05:40 作者: 圓木可阻礙 時(shí)間: 2025-3-26 11:38
Utilization of Cell-Penetrating Peptides for In Vivo Delivery of Bioactive Cargo: The Effect of Nano determined by their physical properties. Therefore, generation of stable particles with strictly defined characteristics is highly essential. Here we describe a formulation protocol of stable and homogenous CPP/pDNA nanoparticles for in vivo applications.作者: 高貴領(lǐng)導(dǎo) 時(shí)間: 2025-3-26 16:27 作者: 鄙視讀作 時(shí)間: 2025-3-26 20:45 作者: –FER 時(shí)間: 2025-3-26 23:29
Magdalene Marinaki,Markos Papageorgioudness complicates identification of candidates suited best for a specific application. To facilitate the screening of this enormous molecular space in a supervised manner we here present a method to “breed” the desired molecules by applying the rules of Darwinian evolution. With this mate-and-check 作者: miniature 時(shí)間: 2025-3-27 04:40
Flow Control in Sewer Networks,ng drug delivery vehicles. Numerous in silico tools have been developed over the years to design and predict the cell-penetrating peptides that contain natural amino acids. The majority of natural cell-penetrating peptides have several limitations including stability, immunogenicity as well as got e作者: BLINK 時(shí)間: 2025-3-27 08:19 作者: palpitate 時(shí)間: 2025-3-27 12:42 作者: 寡頭政治 時(shí)間: 2025-3-27 13:54 作者: cochlea 時(shí)間: 2025-3-27 20:27
Modelling of Sewer Network Flow, EVs are able to transfer their contents (including nucleic acids, proteins, lipids, and small molecules) to recipient cells, and thus hold great promise as drug delivery vehicles. However, their therapeutic application is limited by lack of efficient cargo loading strategies, a need to improve EV t作者: Vulnerary 時(shí)間: 2025-3-27 23:33 作者: Vertebra 時(shí)間: 2025-3-28 02:40
Pekka Neittaanmaki,Dan Tiba,Jürgen Sprekelsal properties, they are classified as three main groups, namely hydrophobic, amphipathic, and hydrophilic. They are capable of interacting with the cell membrane without inducing serious toxicity, and they can carry cargo molecules across the membrane. Cargo molecules could be different therapeutics作者: Assemble 時(shí)間: 2025-3-28 08:14 作者: jocular 時(shí)間: 2025-3-28 13:30
Optimization of Elliptic Systemsinvolves electrostatic anchoring followed by endocytosis, the mode of action of these transporters remains elusive in many cases, and proper understanding of mechanisms behind their penetrating capabilities necessarily entails structural data at the nanoscopic scale. In this chapter, we examine the 作者: 提升 時(shí)間: 2025-3-28 16:50 作者: Minikin 時(shí)間: 2025-3-28 21:49
Optimization of Elliptic Systemshe distinct subcellular localization of them, and their linear counterparts, remains a challenge. Here we describe an optimized method for recombinant generation and purification of eGFP attached to the cyclic form of the newly discovered CPP EJP18 in . We also demonstrate a novel microscopy method 作者: Infantry 時(shí)間: 2025-3-29 02:04
https://doi.org/10.1007/b138797 C′ terminus and the amino acid sequence except for lysines that in PepFect14 are substituted with ornithines. Being non-proteinogenic amino acids, ornithines make PepFect14 less sensitive to serum proteases and due to its positive charges the CPP can form complexes with negatively charged cargos, s作者: 致敬 時(shí)間: 2025-3-29 03:32
Optimization of Curved Mechanical Systems, determined by their physical properties. Therefore, generation of stable particles with strictly defined characteristics is highly essential. Here we describe a formulation protocol of stable and homogenous CPP/pDNA nanoparticles for in vivo applications.作者: 表兩個(gè) 時(shí)間: 2025-3-29 11:03 作者: 圓錐 時(shí)間: 2025-3-29 14:56
Cell Penetrating Peptides978-1-0716-1752-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Fracture 時(shí)間: 2025-3-29 16:28
Optimization of Curved Mechanical Systems, determined by their physical properties. Therefore, generation of stable particles with strictly defined characteristics is highly essential. Here we describe a formulation protocol of stable and homogenous CPP/pDNA nanoparticles for in vivo applications.作者: 松馳 時(shí)間: 2025-3-29 21:38
https://doi.org/10.1007/978-1-0716-1752-6virtual screening; nanoparticles; Arginine-Rich Cell-Penetrating Peptide; antisense oligonucleotides; Si作者: commodity 時(shí)間: 2025-3-30 00:52
978-1-0716-1754-0The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines作者: FEMUR 時(shí)間: 2025-3-30 04:03 作者: EXALT 時(shí)間: 2025-3-30 08:16 作者: 厚臉皮 時(shí)間: 2025-3-30 15:05 作者: nautical 時(shí)間: 2025-3-30 20:24
In Silico Design of Chemically Modified Cell-Penetrating Peptidesng drug delivery vehicles. Numerous in silico tools have been developed over the years to design and predict the cell-penetrating peptides that contain natural amino acids. The majority of natural cell-penetrating peptides have several limitations including stability, immunogenicity as well as got e作者: RENIN 時(shí)間: 2025-3-30 21:46 作者: Neolithic 時(shí)間: 2025-3-31 04:51
A Native CPP from Rattlesnake with Therapeutic and Theranostic Propertiestides can rely on a high content of positive charges, as it is the case of the native CPP crotamine. Crotamine is a polypeptide of about 42 amino acid residues with high content of basic residues as Arg and Lys. Although most of known CPPs are linear peptides,?native crotamine from the venom of a So作者: Lasting 時(shí)間: 2025-3-31 08:54 作者: 使激動(dòng) 時(shí)間: 2025-3-31 09:42
Enhancing the Therapeutic Potential of Extracellular Vesicles Using Peptide Technology EVs are able to transfer their contents (including nucleic acids, proteins, lipids, and small molecules) to recipient cells, and thus hold great promise as drug delivery vehicles. However, their therapeutic application is limited by lack of efficient cargo loading strategies, a need to improve EV t作者: 審問 時(shí)間: 2025-3-31 15:10 作者: 迷住 時(shí)間: 2025-3-31 17:48
Membrane Molecular Interactions and Induced Structures of CPPsal properties, they are classified as three main groups, namely hydrophobic, amphipathic, and hydrophilic. They are capable of interacting with the cell membrane without inducing serious toxicity, and they can carry cargo molecules across the membrane. Cargo molecules could be different therapeutics作者: 小步舞 時(shí)間: 2025-4-1 00:23 作者: 倔強(qiáng)不能 時(shí)間: 2025-4-1 02:19
Methods for Small-Angle Scattering Measurements on Peptiplexes of DNA with Cell-Penetrating Peptidesinvolves electrostatic anchoring followed by endocytosis, the mode of action of these transporters remains elusive in many cases, and proper understanding of mechanisms behind their penetrating capabilities necessarily entails structural data at the nanoscopic scale. In this chapter, we examine the
