作者: 裂口 時(shí)間: 2025-3-21 22:33
Model Organisms for Studying the Cell Cycle, a conserved theme of cell cycle regulation has emerged from research in diverse model organisms. A comparison of distinct features of several diverse model organisms commonly used in cell cycle studies highlights their suitability for various experimental approaches, and recaptures their contribut作者: 惡意 時(shí)間: 2025-3-22 01:27
Role of Computational Modeling in Understanding Cell Cycle Oscillatorsamical systems, such as oscillators, have been investigated by various theoretical and computational modeling methods. Thanks to the insights achieved by these modeling efforts we have gained considerable insights about the underlying molecular regulatory networks that can drive cell cycle oscillati作者: 輕快走過 時(shí)間: 2025-3-22 07:39 作者: Counteract 時(shí)間: 2025-3-22 12:43 作者: 脖子 時(shí)間: 2025-3-22 14:49 作者: 脖子 時(shí)間: 2025-3-22 19:06 作者: PAEAN 時(shí)間: 2025-3-22 22:41 作者: Hemoptysis 時(shí)間: 2025-3-23 02:37
Cell Cycle Dynamics of Proteins and Post-translational Modifications Using Quantitative Immunofluored immunofluorescence provides only a still picture and thus lacks temporal information. Here, we describe a method to extract temporal information from immunofluorescence images of fixed cells. In addition, we provide an optional protocol that uses micropatterns, which increases the accuracy of the 作者: 法律 時(shí)間: 2025-3-23 08:37
Building a Synthetic Transcriptional OscillatorA polymerase and a ribonuclease. The oscillatory behavior of the genelets is driven by the periodic production and degradation of RNA effector molecules. Here, we describe the preparation, assembly, and testing of a synthetic, transcriptional two-node negative-feedback oscillator, whose dynamics can作者: 鎮(zhèn)壓 時(shí)間: 2025-3-23 12:47
The Use of SNAP Labeling to Study Cell Cycle Oscillatory Proteinstion and an equal distribution of chromosomes to provide genomic stability and avoid tumorigenesis. To study mitotic control at the metaphase-to-anaphase transition, a histone H2-GFP-based reporter system was established, allowing simultaneous monitoring of the alignment of mitotic chromosomes and c作者: Mortar 時(shí)間: 2025-3-23 15:48 作者: 坦白 時(shí)間: 2025-3-23 19:36
Measuring Activity and Specificity of Protein Phosphatasespposing kinase and protein phosphatase activities is often complex and major challenges exist in identifying the direct substrates of these enzymes and the specific sites at which they act. While cell cycle kinases are known to exhibit strict substrate specificities important for coordinating the co作者: Minuet 時(shí)間: 2025-3-24 01:12
Combining the Optimized Yeast Cytosine Deaminase Protein Fragment Complementation Assay and an In Viraordinary temporal and spatial specificity by complexing with one of the nine cyclin regulatory subunits. The identification of the cyclin required for targeting Cdk1 to a substrate can help to place the regulation of that protein at a specific time point during the cell cycle and reveal informatio作者: spondylosis 時(shí)間: 2025-3-24 04:33 作者: effrontery 時(shí)間: 2025-3-24 09:37
Detection of Protein–Protein Interactions in Tobacco BY-2 Cells Using Bimolecular Fluorescence Compl the methods available for the study of these interactions, such as yeast two-hybrid and co-immunoprecipitation assays, rely on in vitro techniques. Here we describe the use of bimolecular fluorescence complementation for the study of protein–protein interactions in vivo, using simple techniques and作者: 親屬 時(shí)間: 2025-3-24 12:44 作者: 遺傳學(xué) 時(shí)間: 2025-3-24 18:23
Brian Greer,Swapna Mukhopadhyayons. Here we review the basic features and characteristics of biological oscillators, discussing from a computational modeling point of view their specific architectures and the current knowledge about the dynamics that the life evolution selected to drive cell cycle oscillations.作者: abduction 時(shí)間: 2025-3-24 20:03
On Meaning and Mental Representation from mice present the opportunity to study the effects of defined genetic modifications on a normal cell cycle. However, synchronization of these cells has often been challenging. In this chapter we outline three basic protocols for isolating mouse fibroblasts at the G1-to-S-phase transition, in S phase, and during mitosis.作者: Geyser 時(shí)間: 2025-3-24 23:37
Understanding Standardized Testsyclin B proteolysis. To depict the proteolytic profile, a chimeric cyclin B-SNAP reporter molecule that can be labeled with a fluorochrome-carrying SNAP substrate was generated for measurement of the decline in fluorescence intensity via live-cell imaging. This reporter system can be adapted for other cell cycle oscillatory proteins.作者: 不可比擬 時(shí)間: 2025-3-25 05:36 作者: 單調(diào)女 時(shí)間: 2025-3-25 10:27
Role of Computational Modeling in Understanding Cell Cycle Oscillatorsons. Here we review the basic features and characteristics of biological oscillators, discussing from a computational modeling point of view their specific architectures and the current knowledge about the dynamics that the life evolution selected to drive cell cycle oscillations.作者: Intact 時(shí)間: 2025-3-25 13:13 作者: 談判 時(shí)間: 2025-3-25 19:20
The Use of SNAP Labeling to Study Cell Cycle Oscillatory Proteinsyclin B proteolysis. To depict the proteolytic profile, a chimeric cyclin B-SNAP reporter molecule that can be labeled with a fluorochrome-carrying SNAP substrate was generated for measurement of the decline in fluorescence intensity via live-cell imaging. This reporter system can be adapted for other cell cycle oscillatory proteins.作者: 腐蝕 時(shí)間: 2025-3-25 22:43 作者: Nutrient 時(shí)間: 2025-3-26 00:42 作者: Wallow 時(shí)間: 2025-3-26 04:49
Unaware That I was Walking Backwardsorylation during cell cycle progression in live single cell assays. Because this approach proved to be powerful, we provide here guidelines for the development of biosensors for any phosphorylation site of interest.作者: 運(yùn)氣 時(shí)間: 2025-3-26 08:50
Understanding Where You Came Fromycle TF, our method also assigned specific cell cycle phases in which the TF functions and identified the time lag for the TF to exert regulatory effects on its target genes. Moreover, our method can identify novel cell cycle-regulated genes as a by-product.作者: anthropologist 時(shí)間: 2025-3-26 13:16
Spatiotemporal Investigation of Phosphorylation Events During Cell Cycle Progressionorylation during cell cycle progression in live single cell assays. Because this approach proved to be powerful, we provide here guidelines for the development of biosensors for any phosphorylation site of interest.作者: Lyme-disease 時(shí)間: 2025-3-26 20:36 作者: 有效 時(shí)間: 2025-3-26 22:36 作者: Veneer 時(shí)間: 2025-3-27 04:23 作者: Restenosis 時(shí)間: 2025-3-27 05:23 作者: ANTIC 時(shí)間: 2025-3-27 11:53
Combining the Optimized Yeast Cytosine Deaminase Protein Fragment Complementation Assay and an In Vireliance of the OyCD interaction between Cdk1 and budding yeast γ-tubulin on the Clb3 cyclin, relative to the mitotic Clb2 cyclin, was performed by an in vitro kinase assay using the γ-tubulin complex as a substrate.作者: 核心 時(shí)間: 2025-3-27 14:49
Book 2016ell Cycle Oscillators: Methods and Protocols .will serve as an invaluable reference to gain further insight into the complex and incompletely understood processes that are involved in the cell cycle and its regulation by oscillatory networks..作者: 寬大 時(shí)間: 2025-3-27 20:15
1064-3745 ation advice from the experts? This volume brings together a unique collection of protocols that cover standard, novel, and specialized techniques. .Cell Cycle Oscillators: Methods and Protocols. guides readers through recent progress in the field from both holistic and reductionist perspectives, pr作者: LUMEN 時(shí)間: 2025-3-27 22:41
The Trademan’s Door to the Ivory Towerm immunofluorescence images of fixed cells. In addition, we provide an optional protocol that uses micropatterns, which increases the accuracy of the method. These methods allow assessing how protein levels, intracellular localization, and post-translational modifications change through the cell cycle.作者: Pandemic 時(shí)間: 2025-3-28 03:24
Understanding Where You Came Fromes. Here, we describe the preparation, assembly, and testing of a synthetic, transcriptional two-node negative-feedback oscillator, whose dynamics can be followed in real-time by fluorescence read-out.作者: alabaster 時(shí)間: 2025-3-28 07:36
Cell Cycle Dynamics of Proteins and Post-translational Modifications Using Quantitative Immunofluorem immunofluorescence images of fixed cells. In addition, we provide an optional protocol that uses micropatterns, which increases the accuracy of the method. These methods allow assessing how protein levels, intracellular localization, and post-translational modifications change through the cell cycle.作者: 開花期女 時(shí)間: 2025-3-28 12:27
Building a Synthetic Transcriptional Oscillatores. Here, we describe the preparation, assembly, and testing of a synthetic, transcriptional two-node negative-feedback oscillator, whose dynamics can be followed in real-time by fluorescence read-out.作者: PAGAN 時(shí)間: 2025-3-28 18:21 作者: 鐵砧 時(shí)間: 2025-3-28 22:36
The Usa Mathematics Advisory Panelantitative methodologies, and approaches of systems biology are redefining the traditional concept of what constitutes a model organism and have established a new era for development of novel, and refinement of the established model organisms. Researchers working in the field are no longer separated作者: blithe 時(shí)間: 2025-3-29 00:20 作者: LAIR 時(shí)間: 2025-3-29 05:53
Appreciating Group Processes and Dynamicsndividual phosphorylation sites on physiologically relevant substrates. Here, using the cell cycle phosphatase Cdc14 as an example, we describe two methods for studying phosphatase specificity, one using synthetic phosphopeptide substrates and the other using intact phosphoprotein substrates. These 作者: COLIC 時(shí)間: 2025-3-29 08:27 作者: Gingivitis 時(shí)間: 2025-3-29 12:32
1064-3745 lex and incompletely understood processes that are involved in the cell cycle and its regulation by oscillatory networks..978-1-4939-4958-8978-1-4939-2957-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 流動(dòng)性 時(shí)間: 2025-3-29 17:52 作者: commute 時(shí)間: 2025-3-29 20:39
Model Organisms for Studying the Cell Cycleantitative methodologies, and approaches of systems biology are redefining the traditional concept of what constitutes a model organism and have established a new era for development of novel, and refinement of the established model organisms. Researchers working in the field are no longer separated作者: Fraudulent 時(shí)間: 2025-3-30 00:32 作者: 樂章 時(shí)間: 2025-3-30 07:45 作者: Palatial 時(shí)間: 2025-3-30 11:44 作者: Cerebrovascular 時(shí)間: 2025-3-30 13:09
https://doi.org/10.1007/978-1-4939-2957-3cell cycle; cell division cycle; living systems; oscillatory networks; proliferative diseases; reproducti作者: 起來了 時(shí)間: 2025-3-30 17:15 作者: Generalize 時(shí)間: 2025-3-31 00:02
Gelsa Knijnik,Fernanda Wandererllators comprising cyclin-dependent kinases and other kinases. Different cell cycle oscillators are inextricably linked to ensure orderly activation of oscillators. A recurring theme in their regulation is the abundance of auto-amplifying loops that ensure switch-like and unidirectional cell cycle t作者: PRISE 時(shí)間: 2025-3-31 02:47 作者: Palliation 時(shí)間: 2025-3-31 05:24
Brian Greer,Swapna Mukhopadhyayamical systems, such as oscillators, have been investigated by various theoretical and computational modeling methods. Thanks to the insights achieved by these modeling efforts we have gained considerable insights about the underlying molecular regulatory networks that can drive cell cycle oscillati作者: COW 時(shí)間: 2025-3-31 11:33 作者: 得罪人 時(shí)間: 2025-3-31 17:00
On Meaning and Mental Representations complicated by the variable proliferative properties and responses to synchronizing methods of different cancer-derived cell lines. Paradoxically, cell lines with genetic defects in cell cycle control are sometimes chosen because they are amenable to chemical synchronization. Embryonic fibroblasts作者: 保全 時(shí)間: 2025-3-31 19:58 作者: Cervical-Spine 時(shí)間: 2025-4-1 00:45
