標(biāo)題: Titlebook: Catharanthus roseus; Methods and Protocol Vincent Courdavault,Sebastien Besseau Book 2022 The Editor(s) (if applicable) and The Author(s), [打印本頁] 作者: 武士精神 時(shí)間: 2025-3-21 19:47
書目名稱Catharanthus roseus影響因子(影響力)
書目名稱Catharanthus roseus影響因子(影響力)學(xué)科排名
書目名稱Catharanthus roseus網(wǎng)絡(luò)公開度
書目名稱Catharanthus roseus網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Catharanthus roseus被引頻次
書目名稱Catharanthus roseus被引頻次學(xué)科排名
書目名稱Catharanthus roseus年度引用
書目名稱Catharanthus roseus年度引用學(xué)科排名
書目名稱Catharanthus roseus讀者反饋
書目名稱Catharanthus roseus讀者反饋學(xué)科排名
作者: Lipohypertrophy 時(shí)間: 2025-3-21 22:09
Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/222567.jpg作者: Myocyte 時(shí)間: 2025-3-22 00:23
https://doi.org/10.1007/978-1-0716-2349-7Madagascar periwinkle; EMS mutant library; protoplasting; agrotransformation; monoterpene indole alkaloi作者: 光滑 時(shí)間: 2025-3-22 07:40 作者: certain 時(shí)間: 2025-3-22 11:43 作者: 闡明 時(shí)間: 2025-3-22 13:58
From Methylome to Integrative Analysis of Tissue Specificity,ase resolution is whole-genome bisulfite sequencing (WGBS). Here, we describe an improved procedure for WGBS and original bioinformatic workflows applied to unravel tissue-specific variations of the methylome in relation to gene expression and accumulation of secondary metabolites in the medicinal plant ..作者: 闡明 時(shí)間: 2025-3-22 19:04
Studies in Systems, Decision and Controlpatial organization may reach complex sequential multicellular compartmentation. The most complex example so far characterized is the sequential multicellular biosynthesis of the anticancer monoterpene indole alkaloids in .. RNA in situ hybridization has proven a key technological approach to unrave作者: 王得到 時(shí)間: 2025-3-22 22:45
https://doi.org/10.1007/978-3-319-42372-2omics approaches in plants have shown remarkable development over the last several years, and this data has been instrumental in gene discovery efforts for enzymes and transporters involved in metabolism. For metabolomics, Imaging Mass Spectrometry (IMS) is a powerful tool to map the spatial distrib作者: Panacea 時(shí)間: 2025-3-23 03:46
Telmo Ad?o,Luís Magalh?es,Emanuel Peresitable for analyzing small molecules due to the absence of interfering signals in the low . range originating from the organic matrix. Imaging mass spectrometry (IMS) is a versatile imaging approach with high spatial resolution for analyzing various molecular species, but its application depends hea作者: nonchalance 時(shí)間: 2025-3-23 06:47 作者: 使害怕 時(shí)間: 2025-3-23 11:29
Introduction to Off-road Vehicles,llular levels, collectively suggested by RNA in situ hybridization, enzymatic and transcriptomic studies using leaf epidermis, and fluorescent protein tagging studies. Although documented in other plant species, the long-distance transport of iridoid glycosides, such as secologanin, has not been kno作者: 殘忍 時(shí)間: 2025-3-23 17:25
https://doi.org/10.1007/978-3-319-42542-9from secologanin is described via a bioinspired Pictet–Spengler reaction. Mild conditions and purification by crystallization and flash chromatography allow access to the targeted product in fair yield.作者: Ointment 時(shí)間: 2025-3-23 18:57
Frank Gurski,Jochen Rethmann,Eda Yilmazreatly increasing the probabilities of isolating new chemical entities. To pinpoint and streamline the isolation of new Monoterpene Indole Alkaloids (MIAs) in producing plants, we rendered publicly available the MIA database (MIADB), comprising MS. data for ca. 200 structurally diverse MIA, by uploa作者: Aggressive 時(shí)間: 2025-3-23 23:35 作者: 杠桿支點(diǎn) 時(shí)間: 2025-3-24 02:48 作者: Incommensurate 時(shí)間: 2025-3-24 10:29
Marloes Lamain,Jaap Scheerens,Peter Noortto heterologous hosts. Many steps have already been identified using conventional approaches, enlarging the space of known possible chemical steps. In the recent past years, identification of missing steps has been fueled by the generation of genomic and transcriptomic data for nonmodel species. The作者: 彎彎曲曲 時(shí)間: 2025-3-24 13:54 作者: 延期 時(shí)間: 2025-3-24 17:26 作者: 露天歷史劇 時(shí)間: 2025-3-24 19:14
,Renewable Energy Sources—Overview,inblastine from geraniol and tryptophan precursors. Several approaches have been used to identify the enzymes involved in this iconic and remarkably complex pathway. This chapter describes the use of the classic ethyl methanesulfonate (EMS) mutagenesis to create a selfed M2 mutant population, which 作者: Intercept 時(shí)間: 2025-3-25 01:27 作者: Suppository 時(shí)間: 2025-3-25 03:31
Chris McKee,Ima Itikarai,Hugh Daviesase resolution is whole-genome bisulfite sequencing (WGBS). Here, we describe an improved procedure for WGBS and original bioinformatic workflows applied to unravel tissue-specific variations of the methylome in relation to gene expression and accumulation of secondary metabolites in the medicinal p作者: 兩種語言 時(shí)間: 2025-3-25 07:36 作者: 冒號(hào) 時(shí)間: 2025-3-25 13:39 作者: Limousine 時(shí)間: 2025-3-25 17:34
https://doi.org/10.1007/978-3-319-44144-3lant . (Apocynaceae). No stable transformation and regeneration of plantlets have been achieved with a high efficiency in this plant to date. In addition, while virus-mediated transient gene silencing has been reported a decade ago in ., tools for transient overexpression remain scarce. Here, we des作者: VOK 時(shí)間: 2025-3-25 23:59
https://doi.org/10.1007/978-3-319-43908-2Transcriptional regulation is a central piece of the highly valuable monoterpenoid indole alkaloid pathway of .. and the ultimate tool for its understanding and manipulation. Here, we describe the adaptation of the TARGET methodology to identify specific and genome-wide leaf targets of . candidate transcription factors (TFs).作者: 橢圓 時(shí)間: 2025-3-26 01:06 作者: 支架 時(shí)間: 2025-3-26 07:37 作者: 飾帶 時(shí)間: 2025-3-26 09:06 作者: Pelvic-Floor 時(shí)間: 2025-3-26 12:52
Chris McKee,Ima Itikarai,Hugh Daviesase resolution is whole-genome bisulfite sequencing (WGBS). Here, we describe an improved procedure for WGBS and original bioinformatic workflows applied to unravel tissue-specific variations of the methylome in relation to gene expression and accumulation of secondary metabolites in the medicinal plant ..作者: Graduated 時(shí)間: 2025-3-26 18:24
RNA In Situ Hybridization of Paraffin Sections to Characterize the Multicellular Compartmentation ole of most of the nearly 30 genes involved in this pathway. We introduce here a comparison of advantages and drawbacks of in situ hybridization and more popular promoter: GUS strategies. Two main advantages of in situ hybridization are the suitability to any plant species and the direct localization作者: 惹人反感 時(shí)間: 2025-3-26 21:56 作者: Obstacle 時(shí)間: 2025-3-27 03:20
,Semisynthesis of Bis-Indole Alkaloid (?)-Melodinine K Enabled by a Combination of Biotransformationmodified . yeast strain expressing a tabersonine 16-hydroxylase enzyme?to enable regioselective oxidation on multigram scale, and (?)-pachysiphine is produced through stereoselective and regioselective epoxidation of the disubstituted alkene.作者: MONY 時(shí)間: 2025-3-27 08:06 作者: 斜坡 時(shí)間: 2025-3-27 11:55
Discovery and Characterization of Oxidative Enzymes Involved in Monoterpenoid Indole Alkaloid Biosyering enzymes involved in MIA biosynthesis. Here, we discuss the latest progress in understanding the roles of oxidative enzymes in MIA metabolism and describe a bioinformatic and biochemical pipeline to identify, characterize, and make use of these plant biocatalysts.作者: GLEAN 時(shí)間: 2025-3-27 17:19 作者: 扔掉掐死你 時(shí)間: 2025-3-27 21:31 作者: syncope 時(shí)間: 2025-3-27 23:08 作者: malign 時(shí)間: 2025-3-28 04:30 作者: finale 時(shí)間: 2025-3-28 09:02 作者: Ataxia 時(shí)間: 2025-3-28 13:24
Marloes Lamain,Jaap Scheerens,Peter Noortidentified biosynthetic steps can be used as baits to capture the remaining unknown steps. The present protocol shows how supervised machine learning in the form of artificial neural networks (ANNs) can efficiently classify genes as specialized metabolism related or not according to their expression作者: 樂章 時(shí)間: 2025-3-28 16:39
https://doi.org/10.1007/978-3-319-43249-6ering enzymes involved in MIA biosynthesis. Here, we discuss the latest progress in understanding the roles of oxidative enzymes in MIA metabolism and describe a bioinformatic and biochemical pipeline to identify, characterize, and make use of these plant biocatalysts.作者: flourish 時(shí)間: 2025-3-28 21:33
William J. McGuire,Carina J. Fearnleyl expression as the pathway genes, and (3) isolation of homologs of TFs known to regulate structurally similar or diverse specialized metabolites in different plant species. TFs regulating TIA pathway have been isolated using either an individual or a combination of the three approaches. Here we des作者: altruism 時(shí)間: 2025-3-29 01:24
Book 2022 reproducible protocols..?Authoritative and cutting-edge,?.Catharanthus roseus: Methods and Protocols .aims to be a guidebook to all researchers working at characterizing alkaloid biosynthesis and more broadly specialized metabolisms.作者: sinoatrial-node 時(shí)間: 2025-3-29 06:16 作者: 認(rèn)為 時(shí)間: 2025-3-29 09:21
https://doi.org/10.1007/978-3-319-44144-3omoter transactivation studies or transgene overexpression paired with downstream analyses like quantitative PCR or metabolite analysis. Our protocol takes about 16?days from sowing seeds to obtaining the results of the experiment.作者: dainty 時(shí)間: 2025-3-29 14:27
https://doi.org/10.1007/978-3-319-44144-3st, easy to perform, and results in high amount of transient expression in .. This protocol is expected to serve as valuable tool to enhance the in planta characterization of gene functions or even transiently knock-in novel enzymatic activities.作者: 小爭(zhēng)吵 時(shí)間: 2025-3-29 15:39 作者: Hemiparesis 時(shí)間: 2025-3-29 20:31 作者: INERT 時(shí)間: 2025-3-30 03:33
A Rapid and Efficient Vacuum-Based Agroinfiltration Protocol for Transient Gene Overexpression in Lst, easy to perform, and results in high amount of transient expression in .. This protocol is expected to serve as valuable tool to enhance the in planta characterization of gene functions or even transiently knock-in novel enzymatic activities.作者: 共同給與 時(shí)間: 2025-3-30 07:01
1064-3745 ation advice from the experts.This volume provides updated technical approaches that have been developed to characterize monoterpene indole alkaloid metabolism in .C. roseus. from metabolite/gene product localization, alkaloid chemical synthesis, candidate gene prediction, transcription factor chara作者: 陰謀 時(shí)間: 2025-3-30 08:48
https://doi.org/10.1007/978-3-319-42372-2s for enzymes and transporters involved in metabolism. For metabolomics, Imaging Mass Spectrometry (IMS) is a powerful tool to map the spatial distribution of molecules in the?tissue. Here, we describe the methods which we used to reveal where secondary metabolites, primarily alkaloids, localize in . stem and leaf tissues.作者: Urologist 時(shí)間: 2025-3-30 15:13
Carla Oliveira Henriques,Dulce Coelhoown metabolites, more appropriate and precise approaches need to be developed. Here, a procedure to analyze .N-labeled and nonlabeled LC-MS/MS data for identification of monoterpene indole alkaloids was developed.作者: 解凍 時(shí)間: 2025-3-30 20:06
Imaging MS Analysis in ,s for enzymes and transporters involved in metabolism. For metabolomics, Imaging Mass Spectrometry (IMS) is a powerful tool to map the spatial distribution of molecules in the?tissue. Here, we describe the methods which we used to reveal where secondary metabolites, primarily alkaloids, localize in . stem and leaf tissues.作者: Prophylaxis 時(shí)間: 2025-3-30 23:23 作者: Muffle 時(shí)間: 2025-3-31 02:07 作者: 意外的成功 時(shí)間: 2025-3-31 05:51
Introduction to Off-road Vehicles,ock plants. This chapter describes the in vitro cultivation of . plants and grafting techniques to enable studies concerning iridoid/MIA transport between organs. The iridoid and MIA analysis methods are also provided.作者: anthesis 時(shí)間: 2025-3-31 10:40 作者: 癡呆 時(shí)間: 2025-3-31 14:35
Studying Iridoid Transport in , by Grafting,ock plants. This chapter describes the in vitro cultivation of . plants and grafting techniques to enable studies concerning iridoid/MIA transport between organs. The iridoid and MIA analysis methods are also provided.作者: Negotiate 時(shí)間: 2025-3-31 21:16 作者: 混合,攙雜 時(shí)間: 2025-3-31 23:09
Ancestral Sequence Reconstruction for Exploring Alkaloid Evolution,ormation of complex alkaloids arose over evolutionary time. I discuss the steps of ASR, including sequence selection, multiple sequence alignment, tree inference, and the generation and characterization of inferred ancestral enzymes.作者: Palatial 時(shí)間: 2025-4-1 03:36 作者: consent 時(shí)間: 2025-4-1 08:11 作者: GROWL 時(shí)間: 2025-4-1 11:51
