標(biāo)題: Titlebook: Caspases,Paracaspases, and Metacaspases; Methods and Protocol Peter V. Bozhkov,Guy Salvesen Book 2014 Springer Science+Business Media New Y [打印本頁(yè)] 作者: 法庭 時(shí)間: 2025-3-21 18:56
書目名稱Caspases,Paracaspases, and Metacaspases影響因子(影響力)
書目名稱Caspases,Paracaspases, and Metacaspases影響因子(影響力)學(xué)科排名
書目名稱Caspases,Paracaspases, and Metacaspases網(wǎng)絡(luò)公開(kāi)度
書目名稱Caspases,Paracaspases, and Metacaspases網(wǎng)絡(luò)公開(kāi)度學(xué)科排名
書目名稱Caspases,Paracaspases, and Metacaspases被引頻次
書目名稱Caspases,Paracaspases, and Metacaspases被引頻次學(xué)科排名
書目名稱Caspases,Paracaspases, and Metacaspases年度引用
書目名稱Caspases,Paracaspases, and Metacaspases年度引用學(xué)科排名
書目名稱Caspases,Paracaspases, and Metacaspases讀者反饋
書目名稱Caspases,Paracaspases, and Metacaspases讀者反饋學(xué)科排名
作者: 內(nèi)行 時(shí)間: 2025-3-21 21:50
Positional Scanning Substrate Combinatorial Library (PS-SCL) Approach to Define Caspase Substrate Spscribe the protocol for analyzing S4-S2 pockets preferences of caspases using PS-SCL. Additionally, we describe procedures for the identification of optimal substrates sequence after PS-SCL, solid phase synthesis, and purification of selected fluorogenic substrates, as well as their kinetic analysis作者: 暫時(shí)別動(dòng) 時(shí)間: 2025-3-22 04:19 作者: Console 時(shí)間: 2025-3-22 08:21 作者: 輕浮思想 時(shí)間: 2025-3-22 12:38 作者: pulse-pressure 時(shí)間: 2025-3-22 14:23
Methods for the Study of Caspase Activation in the , Oocyte and Egg Extractificantly contributed to these advances. Twenty years ago, Newmeyer and colleagues first showed that the . egg extract, when incubated at room temperature, reconstituted the key molecular events of cellular apoptosis including cytochrome . release, nuclear condensation, internucleosomal fragmentatio作者: pulse-pressure 時(shí)間: 2025-3-22 20:50
Caspase Protocols in Mice apoptotic pathway. Since the discovery of caspases, many methods have been developed to detect their activation and are widely used in basic and clinical studies. In a mouse tissue, caspase activation can be monitored by cleavage of caspase-specific synthetic substrates and by detecting cleaved cas作者: 廣口瓶 時(shí)間: 2025-3-22 22:45
Measurement of Caspase Activation in Mammalian Cell Culturesleaving protein substrates harboring specific target motifs. Basically all biochemical and morphological changes in an apoptotic cell, including cell shrinkage, chromatin condensation, DNA fragmentation, and plasma membrane blebbing, are consequence of caspase-mediated proteolysis. Thus, uncovering 作者: CLASP 時(shí)間: 2025-3-23 02:22
Detection and Measurement of Paracaspase MALT1 Activity the immune response. Oncogenic activation of MALT1 is associated with the development of specific forms of B-cell lymphomas. Through specific cleavage of its substrates, MALT1 controls various aspects of lymphocyte activation, including the activation of transcriptional pathways, the stabilization 作者: Decrepit 時(shí)間: 2025-3-23 06:55
Metacaspase: An Arginine-Specific Peptidaseole of metacaspase in . is still a matter of debate, whereas its peptidase enzymatic activity has been well characterized. Among the different possible expression systems, metacaspase-deficient yeast cells (.) have been instrumental in studying the activity of . metacaspase (LmjMCA). Here, we descri作者: pulse-pressure 時(shí)間: 2025-3-23 10:19 作者: Corporeal 時(shí)間: 2025-3-23 16:58 作者: Cardiac-Output 時(shí)間: 2025-3-23 22:00 作者: Invertebrate 時(shí)間: 2025-3-24 00:56
Preparation of , Seedling Proteomes for Identifying Metacaspase Substrates by N-terminal COFRADIC by comparing the N-terminal proteomes (or N-terminomes) of wild-type plants to transgenic plants not expressing a given metacaspase. In this chapter we describe a protocol for the preparation of plant tissue proteomes, including differential isotopic labelling allowing for a comparison of in vivo N作者: antiquated 時(shí)間: 2025-3-24 03:00 作者: ASSET 時(shí)間: 2025-3-24 09:56 作者: PANIC 時(shí)間: 2025-3-24 11:55
Peter V. Bozhkov,Guy SalvesenIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the expert.Includes supplementary material作者: 緩和 時(shí)間: 2025-3-24 14:54
Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/222387.jpg作者: GUILT 時(shí)間: 2025-3-24 22:52
Positional Scanning Substrate Combinatorial Library (PS-SCL) Approach to Define Caspase Substrate Spscribe the protocol for analyzing S4-S2 pockets preferences of caspases using PS-SCL. Additionally, we describe procedures for the identification of optimal substrates sequence after PS-SCL, solid phase synthesis, and purification of selected fluorogenic substrates, as well as their kinetic analysis.作者: Ptsd429 時(shí)間: 2025-3-25 03:10 作者: modish 時(shí)間: 2025-3-25 05:02 作者: caldron 時(shí)間: 2025-3-25 07:52 作者: Circumscribe 時(shí)間: 2025-3-25 11:57 作者: 雕鏤 時(shí)間: 2025-3-25 17:25 作者: Inflammation 時(shí)間: 2025-3-25 20:30 作者: fabricate 時(shí)間: 2025-3-26 04:01
Rasheedat M. Mahamood,Esther T. Akinlabi apoptotic pathway. Since the discovery of caspases, many methods have been developed to detect their activation and are widely used in basic and clinical studies. In a mouse tissue, caspase activation can be monitored by cleavage of caspase-specific synthetic substrates and by detecting cleaved cas作者: 陶瓷 時(shí)間: 2025-3-26 07:34
Springer Series in Advanced Manufacturingleaving protein substrates harboring specific target motifs. Basically all biochemical and morphological changes in an apoptotic cell, including cell shrinkage, chromatin condensation, DNA fragmentation, and plasma membrane blebbing, are consequence of caspase-mediated proteolysis. Thus, uncovering 作者: Ligneous 時(shí)間: 2025-3-26 12:19 作者: 花束 時(shí)間: 2025-3-26 16:33 作者: 祖?zhèn)髫?cái)產(chǎn) 時(shí)間: 2025-3-26 18:45 作者: 鐵塔等 時(shí)間: 2025-3-26 21:25
On the Decline of the Genteel Virtuesion to their canonical role in apoptosis/programmed cell death. While the budding yeast metacaspase Yca1 has been well characterized for its role in cell death regulation, more recent examinations suggest that the protease may be involved in key processes that increase survival and fitness. More spe作者: apiary 時(shí)間: 2025-3-27 01:33
On the Decline of the Genteel Virtuesplant biology requires a set of robust methods for detection of metacaspase activation and quantitative analysis of corresponding proteolytic activity. Here we describe methods for purification of recombinant metacaspases, measurement of enzymatic activity of recombinant and endogenous metacaspases 作者: ineptitude 時(shí)間: 2025-3-27 08:54 作者: intimate 時(shí)間: 2025-3-27 12:55 作者: 軟膏 時(shí)間: 2025-3-27 13:50
https://doi.org/10.1007/978-3-030-18500-8scribe the protocol for analyzing S4-S2 pockets preferences of caspases using PS-SCL. Additionally, we describe procedures for the identification of optimal substrates sequence after PS-SCL, solid phase synthesis, and purification of selected fluorogenic substrates, as well as their kinetic analysis.作者: moratorium 時(shí)間: 2025-3-27 20:43 作者: 定點(diǎn) 時(shí)間: 2025-3-27 23:24 作者: 描繪 時(shí)間: 2025-3-28 03:05
Operations Research Proceedingsn migration indicative of proteolytic processing. When applied to cells undergoing apoptosis, this unbiased global method provides a snapshot of the topography and magnitude of proteolytic events associated with programmed cell death.作者: 圓木可阻礙 時(shí)間: 2025-3-28 07:41 作者: 音樂(lè)會(huì) 時(shí)間: 2025-3-28 12:53
https://doi.org/10.1007/978-3-030-20419-8we describe a protocol for the preparation of plant tissue proteomes, including differential isotopic labelling allowing for a comparison of in vivo N-terminomes that serves as the starting point for N-terminal COFRADIC studies.作者: Carcinogen 時(shí)間: 2025-3-28 15:56
General In Vitro Caspase Assay Procedures their activation mechanisms, and the identification of their substrates were made possible by the availability of sufficient amounts of enzymatically pure caspases. The current chapter describes at length the expression, purification, and basic enzymatic characterization of apoptotic caspases.作者: 和諧 時(shí)間: 2025-3-28 22:26
Global Identification of Caspase Substrates Using PROTOMAP (Protein Topography and Migration Analysin migration indicative of proteolytic processing. When applied to cells undergoing apoptosis, this unbiased global method provides a snapshot of the topography and magnitude of proteolytic events associated with programmed cell death.作者: REIGN 時(shí)間: 2025-3-29 00:51
Metacaspase: An Arginine-Specific Peptidasee expression systems, metacaspase-deficient yeast cells (.) have been instrumental in studying the activity of . metacaspase (LmjMCA). Here, we describe techniques for purification of LmjMCA and its activity measurement, providing a platform for further identification of LmjMCA substrates.作者: ANNUL 時(shí)間: 2025-3-29 05:24
Preparation of , Seedling Proteomes for Identifying Metacaspase Substrates by N-terminal COFRADICwe describe a protocol for the preparation of plant tissue proteomes, including differential isotopic labelling allowing for a comparison of in vivo N-terminomes that serves as the starting point for N-terminal COFRADIC studies.作者: cumber 時(shí)間: 2025-3-29 08:33
Measurement of Caspase Activation in Mammalian Cell Culturesactivities of unique caspases are key determinants of the apoptotic process. This chapter describes a set of experimental protocols available for characterization, quantification and inhibition of caspase activities in mammalian cell cultures, including immunoblotting, usage of synthetic substrates, flow cytometry, and microscopic techniques.作者: 秘密會(huì)議 時(shí)間: 2025-3-29 14:40 作者: 宮殿般 時(shí)間: 2025-3-29 17:50 作者: 商品 時(shí)間: 2025-3-29 23:05
Robust Evacuation Planning for Urban Areas cell death execution events. If a protein is cleaved by CED-3 in vitro, this protein could be a potential CED-3 substrate in vivo. Here, we describe the method for purification of active CED-3 caspase. We will also describe in vitro assays for determining CED-3 proteolytic activity, CED-3 substrates, and CED-3 cleavage sites in the substrates.作者: Lethargic 時(shí)間: 2025-3-30 02:20
Imaging Oral Biofilm and Plaque,ot full-length, Caspase-3. Although raised against human cleaved Caspase-3, the CC3 antibody cross-reacts in other species and detects cleaved caspases, most notably DrICE and Dcp-1, in .. This protocol describes the procedure for use of the CC3 antibody to detect caspase activity in larval imaginal discs in ..作者: Fillet,Filet 時(shí)間: 2025-3-30 06:24 作者: canvass 時(shí)間: 2025-3-30 10:41 作者: 脖子 時(shí)間: 2025-3-30 14:47
Rasheedat M. Mahamood,Esther T. Akinlabirates such as PARP, lamin A, and cytokeratin-18 can be monitored in tissue sections by immunostaining as well as western blots of tissue extracts. In general, more than one method should be used to ascertain detection of activation of caspases in a mouse tissue.作者: Directed 時(shí)間: 2025-3-30 18:38 作者: 開(kāi)始沒(méi)有 時(shí)間: 2025-3-31 00:00 作者: Hamper 時(shí)間: 2025-3-31 03:48 作者: 前面 時(shí)間: 2025-3-31 06:54
Springer Series in Advanced Manufacturingactivities of unique caspases are key determinants of the apoptotic process. This chapter describes a set of experimental protocols available for characterization, quantification and inhibition of caspase activities in mammalian cell cultures, including immunoblotting, usage of synthetic substrates, flow cytometry, and microscopic techniques.作者: chemoprevention 時(shí)間: 2025-3-31 11:45
Santiago Gallino,Antonio Morenoof mRNAs, and an increase in cellular adhesion. In lymphocytes, the activity of MALT1 is tightly controlled by its inducible monoubiquitination, which promotes the dimerization of MALT1. Here, we describe both in vitro and in vivo assays that have been developed to assess MALT1 activity.作者: 爭(zhēng)議的蘋果 時(shí)間: 2025-3-31 14:26
