作者: 手銬 時(shí)間: 2025-3-21 20:15
Western Array Analysis of Human Atherosclerotic Plaques作者: sperse 時(shí)間: 2025-3-22 04:14 作者: Incompetent 時(shí)間: 2025-3-22 05:43 作者: 有偏見 時(shí)間: 2025-3-22 12:49 作者: 使成整體 時(shí)間: 2025-3-22 13:44
Number Theory and the Periodicity of Matterf proteins that are only secreted by pathological or normal arterial walls. By focusing only on the secreted proteins found in the tissue culture media, there is an intended bias toward those molecules that would have a higher probability of later being found in plasma. Using this approach, we have 作者: 使成整體 時(shí)間: 2025-3-22 20:09 作者: Monolithic 時(shí)間: 2025-3-22 22:16 作者: 帶來的感覺 時(shí)間: 2025-3-23 02:52 作者: declamation 時(shí)間: 2025-3-23 08:21
Characterization of the Human Atheroma Plaque Secretome by Proteomic Analysisf proteins that are only secreted by pathological or normal arterial walls. By focusing only on the secreted proteins found in the tissue culture media, there is an intended bias toward those molecules that would have a higher probability of later being found in plasma. Using this approach, we have 作者: 主講人 時(shí)間: 2025-3-23 13:37
The Proteome of Endothelial CellsWe have selected 233 proteins by cross-matching the gels, and found that 70% showed an increase and 30% a decrease of expression levels in activated cells. Subsequent identification of 35 altered peptides is made by matrix-assisted laser desorption and ionization time-of-flight mass spectrometry, as作者: 允許 時(shí)間: 2025-3-23 16:24
Book 2007lure. Cardiovascular Proteomics: Methods and Protocols covers many of the above aspects of the proteomic approach in the cardiovascular field. This v- ume takes the reader through the complete process of proteomic analysis, from the obtention of specific heart proteins (troponin I) to the new techni作者: hypotension 時(shí)間: 2025-3-23 19:12
Some Extensions of the Lucas Functions, more than twofold and two additional spots related to these spots were detected. Five of the spots were identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry as protein disulfide isomerase, one as 60 kDa heat-shock protein, and two as elongation factor Tu.作者: HAIRY 時(shí)間: 2025-3-23 23:16 作者: opalescence 時(shí)間: 2025-3-24 04:36
Periodic Table of the Elements,ased on solubility at different pHs: (1) cytoplasmic-enriched extract (neutral pH), (2) myofilament-enriched extract (acidic pH), and (3) membrane protein-enriched pellet. Fractionation of heart tissue in this manner provides the basis for in-depth proteomic analysis.作者: 匯總 時(shí)間: 2025-3-24 10:29 作者: 上釉彩 時(shí)間: 2025-3-24 12:45
Analysis of Antihypertensive Drugs in the Heart of Animal Modelsofile of pathological left ventricular hypertrophy in comparison with normal heart in a model of rats and investigated the proteomic changes in response to different antihypertensive regimens in order to elucidate their cardioprotective effects. Here we describe in depth the protocol for this type of study.作者: 調(diào)整 時(shí)間: 2025-3-24 16:40 作者: 外來 時(shí)間: 2025-3-24 21:16 作者: 使成核 時(shí)間: 2025-3-25 01:08 作者: defuse 時(shí)間: 2025-3-25 04:46
https://doi.org/10.1007/978-1-4020-6660-3rgical limitations. The analysis of these tissues would be assisted if contaminating serum albumin could be reduced. This chapter outlines a protocol for the effective reduction of serum albumin levels from human myocardium extracts enriched for soluble cytoplasmic proteins.作者: Cubicle 時(shí)間: 2025-3-25 09:42 作者: 傀儡 時(shí)間: 2025-3-25 13:32 作者: 男學(xué)院 時(shí)間: 2025-3-25 17:11 作者: 修飾語 時(shí)間: 2025-3-25 20:20
Two-Dimensional Polyacrylamide Gel Electrophoresis for Cardiovascular Proteomicsinely can be applied to parallel quantitative expression profiling of large sets of complex protein mixtures, such as those represented by cardiovascular cell and tissue lysates. This chapter details a procedure for large-format 2DE, and its variations, that has been successfully applied in cardiovascular proteomic research.作者: Mechanics 時(shí)間: 2025-3-26 00:37 作者: Oligarchy 時(shí)間: 2025-3-26 05:24 作者: ALERT 時(shí)間: 2025-3-26 08:58 作者: Accolade 時(shí)間: 2025-3-26 14:56 作者: enormous 時(shí)間: 2025-3-26 17:22
Jan C. A. Boeyens,Demetrius C. Levendisllowing the analysis of proteins in their physiologic/ intracellular context, has become the most commonly used preparative/enrichment method. This chapter introduces state-of-the-art subcellular fractionation protocols and briefly discuss their suitability, advantages, and limitations.作者: Herd-Immunity 時(shí)間: 2025-3-26 23:19 作者: Encephalitis 時(shí)間: 2025-3-27 02:50 作者: 拒絕 時(shí)間: 2025-3-27 07:29
Springer Proceedings in Physicsradients to separate the sample proteins, combined with quantitative computer analysis to detect differentially expressed proteins and mass spectrometry technologies to identify proteins of interest. In spite of the development of novel gel-free technologies, 2DE remains the only technique that rout作者: 使聲音降低 時(shí)間: 2025-3-27 13:25 作者: 錯(cuò)事 時(shí)間: 2025-3-27 17:13
Some Extensions of the Lucas Functions,anisms still remain unclear. In order to identify proteins that are involved in ischemia-reperfusion injury, we compared precipitated 100,000. fractions of normal, ischemic, and ischemic-reperfused rat hearts using two-dimensional (2D) difference gel electrophoresis (2D-DIGE). 2D-DIGE is reliable me作者: Iniquitous 時(shí)間: 2025-3-27 20:25
Jan C. A. Boeyens,Demetrius C. Levendispertension is associated with such adverse effects as accelerated arteriosclerosis and pathological left ventricular hypertrophy, among others. The molecular mechanisms affecting left ventricular hypertrophy remain mostly unknown. The advent of proteome profiling has facilitated the elucidation of d作者: CHAR 時(shí)間: 2025-3-27 23:20 作者: 鬧劇 時(shí)間: 2025-3-28 04:53
https://doi.org/10.1007/978-1-4020-6660-3raction of proteins from tissue samples should be designed to maintain a limited dynamic range in the concentration of proteins present. However, in many tissue extracts a high concentration of serum albumin exists from tissue perfusion and/or an inability to effectively rinse the tissue owing to su作者: 特別容易碎 時(shí)間: 2025-3-28 08:27
Jan C. A. Boeyens,Demetrius C. Levendises in low abundant regulatory proteins at the organelle level. Subcellular fractionation, being flexible, adjustable (both in cell and tissues), and allowing the analysis of proteins in their physiologic/ intracellular context, has become the most commonly used preparative/enrichment method. This ch作者: nominal 時(shí)間: 2025-3-28 10:30 作者: 鐵塔等 時(shí)間: 2025-3-28 17:09 作者: SOB 時(shí)間: 2025-3-28 22:46
https://doi.org/10.1007/978-1-4020-6660-3, iron sulphur centers) andat least 46 different proteins. The hydrophobic nature of its membranebound subunits and the complexity of subunit content present a substantial analytical challenge. The complete protein chemical analysis of complex I purified from bovine mitochondria required the resolut作者: 發(fā)出眩目光芒 時(shí)間: 2025-3-29 01:13
https://doi.org/10.1007/978-1-4020-6660-3t the molecular level without changes in abundance and, therefore represent a critical phenomenon to study. The present report elucidates an approach to studying one such posttranslational modification, phosphorylation, which is an important event in a variety of signaling cascades initiated in the 作者: Jacket 時(shí)間: 2025-3-29 04:10 作者: 六邊形 時(shí)間: 2025-3-29 11:07 作者: Stable-Angina 時(shí)間: 2025-3-29 13:37 作者: GLARE 時(shí)間: 2025-3-29 18:22 作者: 退出可食用 時(shí)間: 2025-3-29 22:26 作者: 免除責(zé)任 時(shí)間: 2025-3-30 02:50
Cardiovascular Proteomics978-1-59745-214-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 記憶 時(shí)間: 2025-3-30 05:17
Two-Dimensional Polyacrylamide Gel Electrophoresis for Cardiovascular Proteomicsradients to separate the sample proteins, combined with quantitative computer analysis to detect differentially expressed proteins and mass spectrometry technologies to identify proteins of interest. In spite of the development of novel gel-free technologies, 2DE remains the only technique that rout作者: Pedagogy 時(shí)間: 2025-3-30 10:32
Analyzing the Cardiac Muscle Proteome by Liquid Chromatography-Mass Spectrometry-Based Expression Prse diseases represent a major cause of global morbidity and death. Innovative preventive and therapeutic measures are urgently needed for early detection, categorization, and treatment of patients at risk of cardiomyopathy. These developments will require a more complete understanding of the molecul
