標(biāo)題: Titlebook: Cardiomyocytes; Methods and Protocol Gary R. Skuse,Maureen C. Ferran Book 2015 Springer Science+Business Media New York 2015 Cardiac myocyt [打印本頁(yè)] 作者: introspective 時(shí)間: 2025-3-21 19:59
書目名稱Cardiomyocytes影響因子(影響力)
書目名稱Cardiomyocytes影響因子(影響力)學(xué)科排名
書目名稱Cardiomyocytes網(wǎng)絡(luò)公開(kāi)度
書目名稱Cardiomyocytes網(wǎng)絡(luò)公開(kāi)度學(xué)科排名
書目名稱Cardiomyocytes被引頻次
書目名稱Cardiomyocytes被引頻次學(xué)科排名
書目名稱Cardiomyocytes年度引用
書目名稱Cardiomyocytes年度引用學(xué)科排名
書目名稱Cardiomyocytes讀者反饋
書目名稱Cardiomyocytes讀者反饋學(xué)科排名
作者: 瑣事 時(shí)間: 2025-3-21 21:40
1064-3745 ns key notes and implementation advice from the experts.This volume has been assembled for scientists interested in basic and applied biomedical research directed toward understanding the development, genetics and function of cardiomyocytes.?The methods and protocols contained within it address cell作者: 紀(jì)念 時(shí)間: 2025-3-22 01:50 作者: FLOAT 時(shí)間: 2025-3-22 06:11
James Chesko,Thomas Vedvick,Steve Reed by the yield from initial steps of isolation procedures. This protocol describes an efficient and rapid method for isolation and maintenance of long-term cultures of neonatal murine cardiomyocytes by effectively shortening the trypsin enzyme digestion period and the cardiomyocyte enrichment step.作者: 沙文主義 時(shí)間: 2025-3-22 11:54 作者: extinct 時(shí)間: 2025-3-22 16:53 作者: extinct 時(shí)間: 2025-3-22 20:24 作者: 憤怒事實(shí) 時(shí)間: 2025-3-22 21:18
Generating Primary Cultures of Murine Cardiac Myocytes and Cardiac Fibroblasts to Study Viral Myocatailed protocols for generation and characterization of primary cultures of murine cardiac myocytes and cardiac fibroblasts, offering a powerful tool to probe cell type-specific responses that determine protection against viral myocarditis.作者: Aspirin 時(shí)間: 2025-3-23 02:58 作者: infantile 時(shí)間: 2025-3-23 07:02 作者: BALE 時(shí)間: 2025-3-23 10:24 作者: Glossy 時(shí)間: 2025-3-23 14:53 作者: 構(gòu)成 時(shí)間: 2025-3-23 21:27 作者: 大洪水 時(shí)間: 2025-3-24 00:56
Leukotrienes and allergic asthma,ing cardiomyocytes have expected molecular, cellular, and electrophysiological characteristics. In this chapter, we describe in detail the differentiation protocol and follow-up characterization focusing on immunocytochemistry, quantitative RT-PCR, and flow cytometry analysis.作者: 確定 時(shí)間: 2025-3-24 05:36
Henri Michael von Blanquet,Michael Friebedroplets on the lid of culture dishes following a 2-day incubation, yield embryoid bodies, which are resuspended and plated. 1–2 weeks following plating of the EBs, spontaneous beating areas can be observed and staining for specific cardiac markers can be achieved.作者: Medicaid 時(shí)間: 2025-3-24 09:06 作者: Armada 時(shí)間: 2025-3-24 14:11
Mouse Embryonic Stem Cell-Derived Cardiac Myocytes in a Cell Culture Dish,droplets on the lid of culture dishes following a 2-day incubation, yield embryoid bodies, which are resuspended and plated. 1–2 weeks following plating of the EBs, spontaneous beating areas can be observed and staining for specific cardiac markers can be achieved.作者: 在駕駛 時(shí)間: 2025-3-24 18:52 作者: BIPED 時(shí)間: 2025-3-24 22:33
Methods of Myofibrillogenesis Modeling,ate different aspects of myofibrillogenesis. We present a specific example for a cardiomyocyte model, but the same procedure can be used to model fibrillogenesis with other mechanisms such as motility. In sum, the models allow for a better understanding of mechanisms behind self-assembly.作者: kidney 時(shí)間: 2025-3-25 02:52
Parminder Singh,Guohua Chen,Wade Worshamportant in the study of inherited and complex traits such as cardiovascular diseases (CVDs). Indeed, the identification and characterization of genes that enhance prediction of CVDs risk remain an important challenge for improving prevention and treatment.作者: 場(chǎng)所 時(shí)間: 2025-3-25 05:23 作者: arbiter 時(shí)間: 2025-3-25 09:57 作者: Oration 時(shí)間: 2025-3-25 11:42 作者: Proponent 時(shí)間: 2025-3-25 17:43
Isolation, Culturing, and Characterization of Cardiac Muscle Cells from Nonhuman Primate Heart Tissc muscle cells by flow cytometry. Based on the location within the heart tissue chosen for cell isolation, a dividing population of cardiac muscle cells expressing cardiomyocyte cell markers was obtained.作者: deviate 時(shí)間: 2025-3-25 21:16
Ankur Singh,Pallab Pradhan,Krishnendu Roystressed and failing hearts, to determine disease-induced changes in microRNAs, mRNAs, and the mRNAs targeted to the RISC, without cross-linking mRNAs to RISC proteins. Deep sequencing methods are used to determine RNA abundances, delivering unbiased, quantitative RNA data limited only by their anno作者: upstart 時(shí)間: 2025-3-26 01:23
Russell Pachynski,Holbrook Kohrtfactory because the yield and phenotypic stability of the hiPSC-ECs are low, and the hiPSC-CMs are often purified via selection for expression of a promoter-reporter construct. In this chapter, we describe an hiPSC-EC differentiation protocol that generates large numbers of stable ECs and an hiPSC-C作者: 你敢命令 時(shí)間: 2025-3-26 06:34
From SICKCARE to HEALTHCARE to HEALTHc muscle cells by flow cytometry. Based on the location within the heart tissue chosen for cell isolation, a dividing population of cardiac muscle cells expressing cardiomyocyte cell markers was obtained.作者: 急急忙忙 時(shí)間: 2025-3-26 12:15 作者: Gossamer 時(shí)間: 2025-3-26 15:05 作者: ostensible 時(shí)間: 2025-3-26 19:53 作者: 共同時(shí)代 時(shí)間: 2025-3-26 21:26 作者: Postulate 時(shí)間: 2025-3-27 04:13
Deep Sequencing of Cardiac MicroRNA-mRNA Interactomes in Clinical and Experimental Cardiomyopathy,art to stress stimuli. As the sequence-specific recognition element of RNA-induced silencing complexes (RISCs), microRNAs bind mRNAs and prevent their translation via mechanisms that may include transcript degradation and/or prevention of ribosome binding. Short microRNA sequences and the ability of作者: 名義上 時(shí)間: 2025-3-27 07:27
Next-Generation Sequencing Technology in the Genetics of Cardiovascular Disease,sible way for obtaining global genomic information. At present, three most NGS platforms are used in genetics for clonally amplified templates. These technologies share general processing steps but differing in specific technical details that determine their limits or advantages. NGS has been recent作者: SPER 時(shí)間: 2025-3-27 13:22
Computational Cardiac Electrophysiology: Implementing Mathematical Models of Cardiomyocytes to Simumake predictions requires solving the models computationally using numerical schemes. We discuss different solution methods and other computational considerations for simulating cardiac action potentials in single cells and multicellular preparations.作者: Throttle 時(shí)間: 2025-3-27 14:34 作者: 讓空氣進(jìn)入 時(shí)間: 2025-3-27 17:57
Using the Mechanical Bidomain Model to Analyze the Biomechanical Behavior of Cardiomyocytes, extracellular matrix and the intracellular cytoskeleton of cardiomyocytes. The goal of this chapter is to introduce the mechanical bidomain model, to describe the mathematical methods required to solve the model equations, and to predict where the membrane forces acting on integrin proteins couplin作者: 的染料 時(shí)間: 2025-3-27 23:50 作者: 使糾纏 時(shí)間: 2025-3-28 02:48 作者: ineptitude 時(shí)間: 2025-3-28 07:33 作者: 表示向前 時(shí)間: 2025-3-28 11:36
Mouse Embryonic Stem Cell-Derived Cardiac Myocytes in a Cell Culture Dish,ee germ layers. In the absence of differentiation inhibitory factors, when cultured in suspension, ES cells spontaneously differentiate and form three-dimensional cell aggregates termed embryoid bodies (EBs). Although various methods exist for the generation of EBs, the hanging drop method offers re作者: 菊花 時(shí)間: 2025-3-28 16:52
Cryopreservation of Neonatal Cardiomyocytes,oliferative abilities, must be performed frequently. To reduce the time requirements and the impact on research animals, we describe a method for cryopreserving neonatal rat cardiomyocytes (NRCMs), and subsequently thawing them for use in assays.作者: enlist 時(shí)間: 2025-3-28 21:34 作者: medieval 時(shí)間: 2025-3-29 02:44 作者: Palatial 時(shí)間: 2025-3-29 03:52 作者: Seizure 時(shí)間: 2025-3-29 07:54
Gary R. Skuse,Maureen C. FerranIncludes cutting-edge methods and protocols in cardiomyocyte research.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: definition 時(shí)間: 2025-3-29 14:25 作者: machination 時(shí)間: 2025-3-29 19:11 作者: 異教徒 時(shí)間: 2025-3-29 21:17
James Chesko,Thomas Vedvick,Steve Reedtain because they do not proliferate after birth. The maintenance of primary cultures of viable and functional cardiomyocytes is considerably affected by the yield from initial steps of isolation procedures. This protocol describes an efficient and rapid method for isolation and maintenance of long-作者: 歡呼 時(shí)間: 2025-3-30 03:53
Ankur Singh,Pallab Pradhan,Krishnendu Royart to stress stimuli. As the sequence-specific recognition element of RNA-induced silencing complexes (RISCs), microRNAs bind mRNAs and prevent their translation via mechanisms that may include transcript degradation and/or prevention of ribosome binding. Short microRNA sequences and the ability of作者: 消音器 時(shí)間: 2025-3-30 05:44
Parminder Singh,Guohua Chen,Wade Worshamsible way for obtaining global genomic information. At present, three most NGS platforms are used in genetics for clonally amplified templates. These technologies share general processing steps but differing in specific technical details that determine their limits or advantages. NGS has been recent作者: 整潔 時(shí)間: 2025-3-30 11:42 作者: HUMID 時(shí)間: 2025-3-30 14:50 作者: Physiatrist 時(shí)間: 2025-3-30 19:07 作者: 聯(lián)合 時(shí)間: 2025-3-30 23:44 作者: 潛伏期 時(shí)間: 2025-3-31 01:12 作者: Fresco 時(shí)間: 2025-3-31 06:14 作者: Expiration 時(shí)間: 2025-3-31 10:40
Henri Michael von Blanquet,Michael Friebeee germ layers. In the absence of differentiation inhibitory factors, when cultured in suspension, ES cells spontaneously differentiate and form three-dimensional cell aggregates termed embryoid bodies (EBs). Although various methods exist for the generation of EBs, the hanging drop method offers re
