作者: BROW 時間: 2025-3-21 22:46 作者: 縱火 時間: 2025-3-22 01:32
Acellular Myocardial Scaffolds and Slices Fabrication, and Method for Applying Mechanical and Electial infarction (MI) treatment. Here, we described an optimal decellularization protocol to generate 3D porcine myocardial scaffolds with well-preserved cardiomyocyte lacunae, myocardial slices as a biomimetic cell culture and delivery platform, and a multi-stimulation bioreactor that is able to prov作者: osteoclasts 時間: 2025-3-22 08:31
,FRESH 3D Bioprinting a Ventricle-like Cardiac Construct Using Human?Stem Cell-Derived Cardiomyocytee embedding of suspended hydrogels (FRESH) 3D bioprinting. To do this, we print a support structure using a collagen type I ink and a cellular component using a high-density cell ink supplemented with fibrinogen. The gelation of the collagen and the fibrinogen into fibrin is initiated by pH change a作者: 愛管閑事 時間: 2025-3-22 10:04
Engineered Heart Tissues for Contractile, Structural, and Transcriptional Assessment of Human Plurivides a useful platform for in vitro cardiac development and disease modeling. This protocol describes the generation of fibrin-based engineered heart tissues (EHTs) containing hiPSC-CMs and human stromal cells. The platform makes use of racks of silicone posts that fit a standard 24-well dish. Stro作者: 水土 時間: 2025-3-22 15:26
High-Throughput Analysis of Drug Safety Responses in Induced Pluripotent Stem Cell-Derived Cardiomyes are seeded to form a cell monolayer on a field of sensors able to detect electrical signals, called extracellular field potentials (EFPs). This noninvasive tool allows researchers to investigate key parameters such as EFP amplitude, duration, and arrhythmias. MEA is progressively considered the g作者: 水土 時間: 2025-3-22 18:05 作者: 使饑餓 時間: 2025-3-22 23:44
,Arrhythmia Assessment in Heterotypic Human Cardiac Myocyte–Fibroblast Microtissues,g a focus on single select ion channels, the use of non-human species in vitro and in vivo, and limited direct physiological translation. To be predictive of actual adverse clinical arrhythmic risk, arrhythmia assessment models for chemicals and drugs should be fit-for-purpose and suited for evaluat作者: Proclaim 時間: 2025-3-23 04:25 作者: 帶來的感覺 時間: 2025-3-23 07:58
Design and Fabrication of Biological Wires for Cardiac Fibrosis Disease Modeling,forms of cardiac disease and has a direct deleterious effect on cardiac function. As currently there is no effective therapeutic strategy specifically designed to target fibrosis, in vitro diagnostic platforms for drug testing have generated significant interest. In this context, we have developed a作者: 強壯 時間: 2025-3-23 10:43
Methods for Transepicardial Cell Transplantation in a Swine Myocardial Infarction Model,ing lost muscle mass and contractile function in injured hearts. Early preclinical work with hPSC-CMs employed rodent models, but the field has recently advanced to transplantation studies in more translationally relevant large animal models including non-human primates and swine. The pig is a parti作者: AGONY 時間: 2025-3-23 16:23
Defined Engineered Human Myocardium for Disease Modeling , Drug Screening, and Heart Repair, 3D engineered versus 2D monolayer and 3D aggregate cardiomyocyte cultures is a clearly advanced degree of maturation, which in many aspects resembles the postnatal rather than the embryonic or fetal heart, in the most advanced 3D culture formats. According to the desired in vitro (disease modeling 作者: ACME 時間: 2025-3-23 21:16 作者: Mystic 時間: 2025-3-24 01:19 作者: corn732 時間: 2025-3-24 03:54 作者: Directed 時間: 2025-3-24 10:24
Encapsulation of Pediatric Cardiac-Derived C-Kit + Cells in Cardiac Extracellular Matrix Hydrogel fts in recent studies and offer multiple benefits, such as easy isolation and autologous transplant. However, concerns about failure of engraftment and transient paracrine effects have thus far limited their use. To overcome these issues, an appropriate cell delivery vehicle such as a cardiac extrace作者: 急急忙忙 時間: 2025-3-24 12:54 作者: gerontocracy 時間: 2025-3-24 16:31
,FRESH 3D Bioprinting a Ventricle-like Cardiac Construct Using Human?Stem Cell-Derived Cardiomyocytels are provided on FRESH support preparation, bioink preparation, dual-extruder needle alignment, print parameter selection, and post-processing. This protocol can also be adapted by altering the 3D model design, cell concentration, or cell type to FRESH 3D bioprint other cardiac tissue constructs.作者: 詼諧 時間: 2025-3-24 19:00
Quantifying Propagation Velocity from Engineered Cardiac Tissues with High-Speed Fluorescence Micro the same procedures can likely be used with human-induced pluripotent stem cell-derived cardiac myocytes, paving the way for patient-specific analysis of propagation due to features such as tissue architecture, substrate rigidity, genetic mutations, or drug treatments.作者: tackle 時間: 2025-3-25 03:08
,Arrhythmia Assessment in Heterotypic Human Cardiac Myocyte–Fibroblast Microtissues, and calcium transients—to 3D heterotypic cardiac myocyte–fibroblast tissues allows for the generation and functional analysis of a large number of individual microtissues to provide greater throughput and high statistical power in analyses. Hundreds of microtissues in standard cell culture plates c作者: 舊石器時代 時間: 2025-3-25 07:05
Methods for Transepicardial Cell Transplantation in a Swine Myocardial Infarction Model,pose of testing hPSC-CM transplantation in a pig model of subacute myocardial infarction, but these same techniques should be broadly applicable to the transepicardial delivery of other biologicals including other candidate cell populations, biomaterials, and/or viral vectors.作者: galley 時間: 2025-3-25 09:01 作者: 遺忘 時間: 2025-3-25 15:44
1064-3745 otocols, Second Edition. serves as an ideal resource for inspiring the advancement of cardiotoxicity assessment, drug discovery, and heart repair and regeneration in order to accelerate heart health improvement around the globe..978-1-0716-2263-6978-1-0716-2261-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: assent 時間: 2025-3-25 16:33 作者: osculate 時間: 2025-3-25 23:06
Successful Local Peacebuilding in Macedonia, the same procedures can likely be used with human-induced pluripotent stem cell-derived cardiac myocytes, paving the way for patient-specific analysis of propagation due to features such as tissue architecture, substrate rigidity, genetic mutations, or drug treatments.作者: 預(yù)防注射 時間: 2025-3-26 03:12
Lesser States and Niche Diplomacy, and calcium transients—to 3D heterotypic cardiac myocyte–fibroblast tissues allows for the generation and functional analysis of a large number of individual microtissues to provide greater throughput and high statistical power in analyses. Hundreds of microtissues in standard cell culture plates c作者: 矛盾心理 時間: 2025-3-26 06:47
https://doi.org/10.1057/9781137429193pose of testing hPSC-CM transplantation in a pig model of subacute myocardial infarction, but these same techniques should be broadly applicable to the transepicardial delivery of other biologicals including other candidate cell populations, biomaterials, and/or viral vectors.作者: 碳水化合物 時間: 2025-3-26 09:32
Nostalgia Marketing and Consumer Behavior,ial injection. Here, we describe the isolation of leukocytes from the heart, blood, and spleen of mice treated with a biomaterial post-myocardial infarction and describe a flow cytometry protocol used to quantify the levels of major leukocyte subtypes, including Ly6C. inflammatory monocytes.作者: 消耗 時間: 2025-3-26 13:13 作者: Venules 時間: 2025-3-26 17:07 作者: incision 時間: 2025-3-27 00:57
Self-Assembled Heterotypic Cardiac Spheroids from Human Pluripotent Stem Cells,r cell–ECM interactions, analogous to early development. Therefore, this approach creates a 3D platform to study how multicellular heterotypic interactions impact tissue structure, function, and phenotype.作者: saphenous-vein 時間: 2025-3-27 03:05
Defined Engineered Human Myocardium for Disease Modeling , Drug Screening, and Heart Repair,or drug screening) and in vivo (heart repair) application, scale and geometry of tissue engineered heart muscle must be adapted. In this updated methods paper, we report a simple and scalable (up and down) collagen-based protocol for the construction of Engineered Human Myocardium (EHM) under defined, serum-free conditions.作者: phytochemicals 時間: 2025-3-27 09:21 作者: GROVE 時間: 2025-3-27 11:55
Book 2022Latest editionnts in cell sourcing, assembly, and use of engineered cardiac tissues, imaging and diagnostics, as well as therapeutic applications. New animal models, biomaterials, and quantitative analyses are described for broad adoption. Written for the highly successful .Methods in Molecular Biology. series, c作者: 戰(zhàn)勝 時間: 2025-3-27 15:54
1064-3745 expertsThis detailed volume presents an updated collection of state-of-the-art protocols in cardiac tissue engineering. These protocols demonstrate advancements in cell sourcing, assembly, and use of engineered cardiac tissues, imaging and diagnostics, as well as therapeutic applications. New anima作者: tattle 時間: 2025-3-27 21:34 作者: 凹室 時間: 2025-3-28 01:15 作者: MURKY 時間: 2025-3-28 05:03 作者: 材料等 時間: 2025-3-28 09:57 作者: 單挑 時間: 2025-3-28 11:52
https://doi.org/10.1007/978-3-319-46150-2he genes to be modified can be curated or whole genomes can be investigated. The use of lentiviral sgRNA delivery followed by high-throughput sequencing allows for rapid comparison and identification of candidate genes and epigenetic modifiers, which can be further validated individually or in sub-pooled libraries following screening.作者: 反應(yīng) 時間: 2025-3-28 16:55 作者: 闖入 時間: 2025-3-28 20:54 作者: 殺菌劑 時間: 2025-3-29 01:36 作者: 驚呼 時間: 2025-3-29 03:10
Successful Local Peacebuilding in Macedonia,ntractility parameters can be derived from these beating atrial EHTs by video-optical monitoring. The presented atrial EHT model is suitable to study chamber-specific mechanisms, drug effects and to serve for disease modeling.作者: 起皺紋 時間: 2025-3-29 08:26 作者: Mechanics 時間: 2025-3-29 13:54
Santiago Ewig,Stathis Phillipou cECM hydrogel to prepare a minimally invasive, dual therapeutic for in vivo delivery. We also outline a detailed method for ultrasound-guided intramyocardial injection of cell-laden hydrogels in a rodent model. Additional steps for labeling cells with a fluorescent dye for in vivo cell tracking are provided.作者: Largess 時間: 2025-3-29 16:03 作者: 分發(fā) 時間: 2025-3-29 19:42
Injectable ECM Scaffolds for Cardiac Repair,ugh the diaphragm. Although the methods shown here are for injection of an acellular biomaterial, cells or other therapeutics could also be added to the injection for testing other regenerative medicine strategies.作者: Encoding 時間: 2025-3-30 02:33 作者: Obligatory 時間: 2025-3-30 04:41 作者: 沒收 時間: 2025-3-30 08:34
https://doi.org/10.1007/978-94-011-7534-0ntractions. The platform described herein is amenable to various measures of cardiac function including measurement of contractile force and calcium handling, as well as molecular biology assays and immunostaining.作者: hardheaded 時間: 2025-3-30 15:12
Successful Local Peacebuilding in Macedonia, is constructed to recapitulate contractile, biomechanical, and electrophysiological complexities of fibrotic myocardium. Additionally, an integrated model with fibrotic and healthy cardiac tissues coupled together can be created to mimic focal fibrosis. The methods for constructing the Biowire fibrotic model will be described here.作者: vector 時間: 2025-3-30 16:41
Rebound: The Return of Norwegian Shipping, using wheat germ agglutinin to measure cardiomyocyte size, nuclear staining to quantify cardiomyocyte nucleation, and Trichrome staining to identify myocardial regeneration and scarring in the myocardium.作者: Maximizer 時間: 2025-3-30 23:53
Engineered Heart Tissues for Contractile, Structural, and Transcriptional Assessment of Human Plurintractions. The platform described herein is amenable to various measures of cardiac function including measurement of contractile force and calcium handling, as well as molecular biology assays and immunostaining.作者: 獸群 時間: 2025-3-31 03:05
Design and Fabrication of Biological Wires for Cardiac Fibrosis Disease Modeling, is constructed to recapitulate contractile, biomechanical, and electrophysiological complexities of fibrotic myocardium. Additionally, an integrated model with fibrotic and healthy cardiac tissues coupled together can be created to mimic focal fibrosis. The methods for constructing the Biowire fibrotic model will be described here.作者: Decline 時間: 2025-3-31 05:22
Quantifying Cardiomyocyte Proliferation and Nucleation to Assess Mammalian Cardiac Regeneration, using wheat germ agglutinin to measure cardiomyocyte size, nuclear staining to quantify cardiomyocyte nucleation, and Trichrome staining to identify myocardial regeneration and scarring in the myocardium.作者: 記成螞蟻 時間: 2025-3-31 11:17 作者: 莎草 時間: 2025-3-31 16:07 作者: 悄悄移動 時間: 2025-3-31 20:09
978-1-0716-2263-6Springer Science+Business Media, LLC, part of Springer Nature 2022作者: Mutter 時間: 2025-4-1 00:34
Kareen L.K. Coulombe,Lauren D. Black IIIIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts作者: 反應(yīng) 時間: 2025-4-1 05:04 作者: Conflagration 時間: 2025-4-1 07:15
