標(biāo)題: Titlebook: Cardiac Tissue Engineering; Methods and Protocol Milica Radisic,Lauren D. Black III Book 2014 Springer Science+Business Media New York 2014 [打印本頁(yè)] 作者: nourish 時(shí)間: 2025-3-21 18:36
書目名稱Cardiac Tissue Engineering影響因子(影響力)
作者: NAUT 時(shí)間: 2025-3-21 21:15 作者: TOXIC 時(shí)間: 2025-3-22 02:25
A Physical Space Powerfully Shaped by Man, presence of intrinsic regulatory factors regulating cardiac function. We describe here how to obtain native ECM hydrogels from porcine hearts for the culture of human embryonic, induced pluripotent, and somatic stem cells for cardiac tissue engineering and regenerative medicine applications.作者: ESPY 時(shí)間: 2025-3-22 07:52 作者: Parallel 時(shí)間: 2025-3-22 11:28 作者: Judicious 時(shí)間: 2025-3-22 16:17
Natural Cardiac Extracellular Matrix Hydrogels for Cultivation of Human Stem Cell-Derived Cardiomyo presence of intrinsic regulatory factors regulating cardiac function. We describe here how to obtain native ECM hydrogels from porcine hearts for the culture of human embryonic, induced pluripotent, and somatic stem cells for cardiac tissue engineering and regenerative medicine applications.作者: Judicious 時(shí)間: 2025-3-22 20:37 作者: Perigee 時(shí)間: 2025-3-22 21:37
Book 2014rchers around the globe. These include methods and protocols for cell preparation, biomaterial preparation, cell seeding, and cultivation in various systems. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, list作者: Spinous-Process 時(shí)間: 2025-3-23 04:07
Book 2014 pitfalls..Authoritative and practical, .Cardiac Tissue Engineering: Methods and Protocols .highlights the major techniques, both experimental and computational, for the study of cardiovascular tissue engineering..作者: chuckle 時(shí)間: 2025-3-23 08:39 作者: Oscillate 時(shí)間: 2025-3-23 13:13
,The Politics of the Past,1998–2008,loped to address the many requirements of an injectable biomaterial in cardiac restoration. This chapter highlights the fundamental aspects of making this biomimetic hydrogel matrix for cardiac applications.作者: 內(nèi)部 時(shí)間: 2025-3-23 16:46 作者: 即席 時(shí)間: 2025-3-23 20:48
Second Generation Codon Optimized Minicircle (CoMiC) for Nonviral Reprogramming of Human Adult Fibr, have recently been developed. Utilizing a newly developed codon optimized 4-in-1 minicircle (CoMiC), we were able to reprogram human adult fibroblasts using chemically defined media and without the need for feeder cells.作者: 外星人 時(shí)間: 2025-3-23 23:42
Fabrication of PEGylated Fibrinogen: A Versatile Injectable Hydrogel Biomaterial,loped to address the many requirements of an injectable biomaterial in cardiac restoration. This chapter highlights the fundamental aspects of making this biomimetic hydrogel matrix for cardiac applications.作者: Lacunar-Stroke 時(shí)間: 2025-3-24 06:02
Cell Sheet Technology for Cardiac Tissue Engineering,nsive culture surfaces, can be easily transplanted onto heart tissues of animal models, and those cell sheet constructs enhance the cell transplant efficiency, resulting in the induction of effective therapy.作者: 磨碎 時(shí)間: 2025-3-24 08:12
Northern Irish Poetry and Theologye introduction of variability in other organ systems has demonstrated beneficial effects to cell function in vitro. We hypothesize that the introduction of variability in engineered cardiac tissue could have a similar effect.作者: 放縱 時(shí)間: 2025-3-24 14:34 作者: 暴行 時(shí)間: 2025-3-24 18:49 作者: 珊瑚 時(shí)間: 2025-3-24 20:32 作者: Pedagogy 時(shí)間: 2025-3-25 01:42 作者: 嫻熟 時(shí)間: 2025-3-25 06:45
https://doi.org/10.1057/9781137271723to modulate cardiomyocyte properties. In this chapter we illustrate the use of the whole-cell patch clamp technique by recording hERG (human ether-à-go-go-related gene) currents and action potentials in ESC-derived cardiomyocytes and also examine the effects of hERG channel blocker, dofetilide.作者: insightful 時(shí)間: 2025-3-25 09:18 作者: 滔滔不絕的人 時(shí)間: 2025-3-25 13:19
Isolation and Expansion of C-Kit-Positive Cardiac Progenitor Cells by Magnetic Cell Sorting,and clonogenic, can be harvested from mature tissue, and have the distinct advantages of autologous transplant and lack of tumor formation in a clinical setting. Here we focus on the isolation, expansion, and myocardial differentiation of rat CPCs. Brief adaptations of the protocol for isolation from mouse and human tissue are also provided.作者: 消息靈通 時(shí)間: 2025-3-25 18:14
Cell Tri-Culture for Cardiac Vascularization,howed microstructural details characteristic of cardiomyocytes and nascent vessels and exhibited synchronous beating activity in vitro. Upon implantation, stable grafts were formed presenting intense vascularization, with evidence of anastomosis between the pre-formed endothelial capillaries and host neovessels.作者: 錯(cuò) 時(shí)間: 2025-3-25 20:46
Patch-Clamp Technique in ESC-Derived Cardiomyocytes,to modulate cardiomyocyte properties. In this chapter we illustrate the use of the whole-cell patch clamp technique by recording hERG (human ether-à-go-go-related gene) currents and action potentials in ESC-derived cardiomyocytes and also examine the effects of hERG channel blocker, dofetilide.作者: 文字 時(shí)間: 2025-3-26 01:47 作者: CIS 時(shí)間: 2025-3-26 08:03
A Physical Space Powerfully Shaped by Man,ed to an alternating magnetic field of 40 Hz for 7 days. The magnetic stimulation per se promoted EC organization into capillary-like structures with no supplementation of angiogenic factors; in the non-stimulated constructs, the cells formed sheets or aggregates..This chapter describes in detail th作者: BULLY 時(shí)間: 2025-3-26 08:36
https://doi.org/10.1057/9780333977866chniques for culturing stem cells and their cardiac derivatives on these substrates. Importantly, this wrinkled cell culture platform is compatible with both fluorescence and bright-field imaging; real-time physiological monitoring of CM action potential propagation and contraction properties can el作者: RALES 時(shí)間: 2025-3-26 15:59
https://doi.org/10.1057/9780333977866diaphragm. Although the methods shown here are for injection of an acellular biomaterial, cells or other therapeutics could also be added to the injection for testing other regenerative medicine strategies.作者: GEON 時(shí)間: 2025-3-26 18:48 作者: 攝取 時(shí)間: 2025-3-27 00:43
1064-3745 .highlights the major techniques, both experimental and computational, for the study of cardiovascular tissue engineering..978-1-4939-4613-6978-1-4939-1047-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 表示向前 時(shí)間: 2025-3-27 04:28
Optogenetic Control of Cardiomyocytes via Viral Delivery,作者: 種類 時(shí)間: 2025-3-27 06:41 作者: 瘙癢 時(shí)間: 2025-3-27 09:48 作者: Esalate 時(shí)間: 2025-3-27 14:14 作者: 盡管 時(shí)間: 2025-3-27 20:36
Injectable ECM Scaffolds for Cardiac Repair,diaphragm. Although the methods shown here are for injection of an acellular biomaterial, cells or other therapeutics could also be added to the injection for testing other regenerative medicine strategies.作者: MAIZE 時(shí)間: 2025-3-28 01:39
Generation of Strip-Format Fibrin-Based Engineered Heart Tissue (EHT),ibrin cell blocks into new 24-well dishes with culture medium. Without further handling, the cells start to remodel the fibrin gel, form contacts with each other, elongate, and condense the gel to approximately ? of the initial volume. Spontaneous and rhythmic contractions start after 1 week. EHTs a作者: lymphoma 時(shí)間: 2025-3-28 03:49 作者: 松軟無(wú)力 時(shí)間: 2025-3-28 06:36 作者: 手工藝品 時(shí)間: 2025-3-28 11:18 作者: Ccu106 時(shí)間: 2025-3-28 16:09 作者: scrutiny 時(shí)間: 2025-3-28 22:48
Cardiac Tissue Engineering978-1-4939-1047-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Cupidity 時(shí)間: 2025-3-29 02:13
Northern Irish Poetry and Theologythe many published methods it appears that hydrogels constitute the preferred scaffolds for myocardial tissue engineering and support of organotypic development. Here we describe a simple and highly robust protocol for the generation of engineered heart muscle using a collagen-based hydrogel method.作者: 帶傷害 時(shí)間: 2025-3-29 05:43
Northern Irish Poetry and the Russian Turndial infarction (MI) treatment. Here we describe an optimal decellularization protocol to generate 3D porcine myocardial scaffolds with well-preserved cardiomyocyte lacunae and a multi-stimulation bioreactor that is able to provide coordinated mechanical and electrical stimulation for facilitating cardiac construct development.作者: 溫順 時(shí)間: 2025-3-29 07:47
The ,: Iceland’s Change of Fashionerative medicine. This technique allows researchers to obtain pluripotent stem cells without the controversial use of embryos, providing a novel and powerful tool for disease modeling and drug screening approaches. However, using viruses for the delivery of reprogramming genes and transcription fact作者: 背信 時(shí)間: 2025-3-29 14:36 作者: FLIT 時(shí)間: 2025-3-29 18:36
https://doi.org/10.1007/978-1-349-15721-1ipheral blood, bone marrow, and cord blood, and are one of the leading candidates for promoting vascularization in tissue engineering therapies. CACs can be isolated by culturing peripheral blood mononuclear cells (PBMCs) on fibronectin or by flow cytometry to obtain more specific subpopulations. He作者: fluffy 時(shí)間: 2025-3-29 23:43
https://doi.org/10.1007/978-1-349-15721-1donor availability. Multiple cell types have been examined for myocardial regeneration, including mesenchymal stem cells (and other bone marrow-derived cells), induced pluripotent stem cells, embryonic stem cells, cardiosphere-derived cells, and cardiac progenitor cells (CPCs). CPCs are multipotent 作者: TRACE 時(shí)間: 2025-3-30 03:42 作者: 冰雹 時(shí)間: 2025-3-30 07:41
,The Politics of the Past,1998–2008,r synthetic polymers, and their high water content gives these materials practical advantages in numerous biomedical applications. Semisynthetic hydrogels, such as those that combine synthetic and biological building blocks, have the added advantage of controlled bioactivity and material properties.作者: Wordlist 時(shí)間: 2025-3-30 11:39
A Physical Space Powerfully Shaped by Man, tissue formation. The use of native extracellular matrix (ECM) is of special interest for the culture of cardiac stem and progenitor cells due to the presence of intrinsic regulatory factors regulating cardiac function. We describe here how to obtain native ECM hydrogels from porcine hearts for the作者: FOIL 時(shí)間: 2025-3-30 12:59 作者: 賞錢 時(shí)間: 2025-3-30 20:00
https://doi.org/10.1057/9780333977866lture. Microenvironmental factors cue structural organization of the myocardium, which promotes the mechanical contractile properties and electrophysiological patterns seen in mature cardiomyocytes. Current nano- and microfabrication techniques, such as photolithography, generate simplified cell cul作者: Nucleate 時(shí)間: 2025-3-30 23:09 作者: Condescending 時(shí)間: 2025-3-31 04:28
,Seamus Heaney: ‘the Appetites of Gravity’,issue engineering method requires cardiomyocytes, a liquid matrix that forms a gel, a casting mold, and a device that keeps the developing tissue in place. This protocol refers to neonatal rat heart cells as the cell source; the matrix of choice is fibrin, and the tissues are generated in rectangula作者: 簡(jiǎn)潔 時(shí)間: 2025-3-31 07:47
https://doi.org/10.1057/9781137330390oach for induction of vascular networks embedded within cardiac tissue constructs. The construct is composed of human cardiomyocytes, endothelial cells (ECs), and embryonic fibroblast cells co-seeded onto highly porous three-dimensional (3D) scaffolds. The resulting vascularized cardiac constructs s作者: 單調(diào)女 時(shí)間: 2025-3-31 13:08
Seamus Heaney: Neighbours and Strangers,sue regeneration. A temperature-responsive culture surface can be fabricated by grafting a temperature-responsive polymer, poly(.-isopropylacrylamide), onto a polystyrene cell culture surface. Cells cultured confluently on such a culture surface can be recovered as an intact cell sheet, and function作者: Truculent 時(shí)間: 2025-3-31 15:48
Northern Irish Poetry and Domestic Spacetforms to study novel small molecules and cardiac disease at the tissue level. Yet, due to the immaturity of hPSC-CMs, there is a low fidelity between tissue-engineered cardiac tissues and adult cardiac tissues. To address this challenge, we have developed a platform that combines both physical and 作者: 系列 時(shí)間: 2025-3-31 20:55
Northern Irish Poetry and Theologythe many published methods it appears that hydrogels constitute the preferred scaffolds for myocardial tissue engineering and support of organotypic development. Here we describe a simple and highly robust protocol for the generation of engineered heart muscle using a collagen-based hydrogel method.作者: Exonerate 時(shí)間: 2025-3-31 23:33 作者: deciduous 時(shí)間: 2025-4-1 02:17
