標(biāo)題: Titlebook: Cardiac Regeneration; Methods and Protocol Kenneth D. Poss,Bernhard Kühn Book 2021 Springer Science+Business Media, LLC, part of Springer N [打印本頁] 作者: Tamoxifen 時(shí)間: 2025-3-21 19:36
書目名稱Cardiac Regeneration影響因子(影響力)
書目名稱Cardiac Regeneration影響因子(影響力)學(xué)科排名
書目名稱Cardiac Regeneration網(wǎng)絡(luò)公開度
書目名稱Cardiac Regeneration網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Cardiac Regeneration被引頻次
書目名稱Cardiac Regeneration被引頻次學(xué)科排名
書目名稱Cardiac Regeneration年度引用
書目名稱Cardiac Regeneration年度引用學(xué)科排名
書目名稱Cardiac Regeneration讀者反饋
書目名稱Cardiac Regeneration讀者反饋學(xué)科排名
作者: 意外的成功 時(shí)間: 2025-3-21 20:54
Apical Resection and Cryoinjury of Neonatal Mouse Hearte regenerative capacity of neonatal mouse hearts, including ventricular amputation, cryoinjury, and ligation of a major coronary artery. While the ventricular resection model can be utilized to study how tissue forms and regenerates de novo, cryoinjury and coronary artery?ligation are methods that m作者: 涂掉 時(shí)間: 2025-3-22 03:02
Left Ventricular Pressure Volume Loop Measurements Using Conductance Catheters to Assess Myocardial sure volume loop recordings greatly enhancing the technique. While a powerful tool, proper execution of the procedure is paramount to ensure reproducibility and reliability of the results obtained. Here, we describe the technique of left ventricular catheterization using the Scisense conductance cat作者: MUMP 時(shí)間: 2025-3-22 04:34
Myocardial Infarction in Pigsarge animal model with clinic-derived instrumentation allows for detailed imitation of interventional catheterization routines and application routes, whereas similar anatomy and heart proportions raise the possibility to precisely evaluate the efficacy of application modes, e.g., antegrade or retro作者: Spirometry 時(shí)間: 2025-3-22 08:54
Ventricular Cryoinjury as a Model to Study Heart Regeneration in Zebrafishbrafish is an excellent model organism to study the cellular and molecular mechanisms promoting heart regeneration. Several cardiac injury models have been developed in zebrafish, including ventricular resection, genetic ablation, and ventricular cryoinjury. This chapter provides a detailed protocol作者: 多骨 時(shí)間: 2025-3-22 14:14
Cardiac Resection Injury in Zebrafishdecades, contributing to the identification of signals and cellular mechanisms as potential targets for human heart repair. Investigations into cardiac regeneration in zebrafish have been facilitated by multiple methods of inducing cardiac tissue damage. Among the established methods, cardiac resect作者: 多骨 時(shí)間: 2025-3-22 19:30
A Genetic Cardiomyocyte Ablation Model for the Study of Heart Regeneration in Zebrafishey model system for studying the cellular and molecular mechanisms of innate heart regeneration. The ease of genetic manipulation in zebrafish has enabled?the establishment of a genetic ablation injury model in which over 60% of cardiomyocytes can be depleted, eliciting signs of heart failure. After作者: 含糊 時(shí)間: 2025-3-22 21:54 作者: installment 時(shí)間: 2025-3-23 03:58
Isolation, Culture, and Live-Cell Imaging of Primary Rat Cardiomyocytesl) as well as other cell types, including endothelial cells and vascular smooth muscle cells. Pericytes, neurons, and immune cells are less abundant, yet still important. Whereas cardiomyocytes account for around 75% of the heart volume, 50–70% of the cells in the heart are non-myocytes. This comple作者: cumulative 時(shí)間: 2025-3-23 07:31 作者: NIP 時(shí)間: 2025-3-23 11:13
Differentiation of Human Induced Pluripotent Stem Cells into Epicardial-Like Cells and smooth muscle populations of the heart and secrete paracrine factors that promote cardiomyocyte proliferation and angiogenesis. Despite a central role in cardiac biology, the mechanisms by which epicardial cells influence cardiac growth are largely unknown, and robust models of the epicardium a作者: 羽毛長成 時(shí)間: 2025-3-23 15:37 作者: GULF 時(shí)間: 2025-3-23 21:01
Frame-Hydrogel Methodology for Engineering Highly Functional Cardiac Tissue Constructs. Here, we describe a versatile “frame-hydrogel” methodology to generate engineered cardiac tissues with highly mature functional properties. This methodology has been successfully utilized with a variety of cell sources (neonatal rat ventricular myocytes, human and mouse pluripotent stem cell-deriv作者: 壯觀的游行 時(shí)間: 2025-3-23 22:15
Efficient Protocols for Fabricating a Large Human Cardiac Muscle Patch from Human Induced Pluripotendisease; however, their full potential cannot be met without robust methods for differentiating them into cardiac-lineage cells. Here, we present novel protocols for generating hiPSC-derived cardiomyocytes (CMs), endothelial cells (ECs), and smooth muscle cells (SMCs) and for assembling them into a 作者: 多山 時(shí)間: 2025-3-24 04:38 作者: 曲解 時(shí)間: 2025-3-24 07:40
Ex Vivo Techniques to Study Heart Regeneration in Zebrafishanisms of cardiac regeneration. Upon injury, the epicardium, the outermost mesothelial tissue layer of vertebrate hearts, serves dual purposes in the regenerating heart as both a signaling center and a source for crucial cell types. Traditional in vivo genetic approaches to study heart regeneration 作者: 倔強(qiáng)不能 時(shí)間: 2025-3-24 12:14 作者: Anal-Canal 時(shí)間: 2025-3-24 18:39 作者: 排出 時(shí)間: 2025-3-24 21:07 作者: 積習(xí)已深 時(shí)間: 2025-3-25 00:06
https://doi.org/10.1057/9780230109056actors, with tissues routinely reaching conduction velocities and specific forces of 25?cm/s and 20?mN/mm., respectively, and forces per input cardiomyocyte of up to 12?nN. This method is reproducible and readily scalable to generate small tissues ideal for in vitro testing as well as tissues with large, clinically relevant dimensions.作者: 流動性 時(shí)間: 2025-3-25 03:30
Aidan Norrie,Carolyn Harris,Elena Woodacreation are monitored in real time after uninjured or injured hearts are excised, washed, and cultured for up to 30?days. In addition to these techniques, we describe ex vivo genetic ablation of the epicardium, cell proliferation assays, partial ventricular explant culturing, and chemical screening.作者: 不如樂死去 時(shí)間: 2025-3-25 08:02
Differentiation of Human Induced Pluripotent Stem Cells into Epicardial-Like Cellstic of the developing epicardium. We also review our protocol for differentiating iECs into fibroblasts and smooth muscle cells through treatment with bFGF and TGF-β1, respectively. iECs provide a platform for studying fundamental epicardial biology and can inform strategies for therapeutic heart regeneration.作者: 引水渠 時(shí)間: 2025-3-25 14:48 作者: 在駕駛 時(shí)間: 2025-3-25 19:23 作者: 障礙物 時(shí)間: 2025-3-25 20:23
https://doi.org/10.1007/978-3-319-74048-5njury models and describe a set of methods used to accurately analyze and quantify cardiac outcomes. Importantly, a comprehensive understanding of cardiac regeneration and repair requires a combination of multiple functional, histological, and molecular analyses.作者: fastness 時(shí)間: 2025-3-26 02:24 作者: Ringworm 時(shí)間: 2025-3-26 07:54
https://doi.org/10.1057/9780230109056ventional methods, while the hCMP fabrication procedure produces a patch of clinically relevant size and incorporates a simple method for maturing the engineered tissue via mechanical stimulation. We also describe how the patch can be evaluated in a large-animal (swine) model of myocardial injury.作者: Vasodilation 時(shí)間: 2025-3-26 09:58
Normative Aspekte der Public Relationstes. Moreover, we describe a cardiac differentiation protocol to derive cardiomyocytes from hiPSCs, as well as a strategy to use fluorescence-activated cell sorting to isolate pure populations of fluorescently labeled cardiomyocytes for downstream applications.作者: FER 時(shí)間: 2025-3-26 13:35 作者: Indigence 時(shí)間: 2025-3-26 19:16 作者: Sedative 時(shí)間: 2025-3-26 22:26 作者: Obstruction 時(shí)間: 2025-3-27 01:41
Purification of Pluripotent Stem Cell-Derived Cardiomyocytes Using CRISPR/Cas9-Mediated Integration tes. Moreover, we describe a cardiac differentiation protocol to derive cardiomyocytes from hiPSCs, as well as a strategy to use fluorescence-activated cell sorting to isolate pure populations of fluorescently labeled cardiomyocytes for downstream applications.作者: 使更活躍 時(shí)間: 2025-3-27 05:22 作者: 單獨(dú) 時(shí)間: 2025-3-27 09:47
https://doi.org/10.1007/978-1-349-02402-5grade intracoronary application of locally acting pharmaceutical agents, viruses, and cells. Here, we describe the techniques of left ventricular catheterization and induction of ischemia and reperfusion, as well as hemodynamic monitoring and regional application of therapeutic agents in pigs.作者: nonplus 時(shí)間: 2025-3-27 17:20 作者: Judicious 時(shí)間: 2025-3-27 21:41 作者: 冰河期 時(shí)間: 2025-3-27 21:57
1064-3745 expertsThis detailed book addresses major goals of regenerative medicine and the cardiovascular research community with techniques to replenish lost cardiomyocytes, avoid scar-associated pathology, and improve myocardial infarction (MI) outcomes. The collection begins with a section on cardiac inju作者: 獨(dú)裁政府 時(shí)間: 2025-3-28 02:28
https://doi.org/10.1007/978-3-319-47042-9bility and reliability of the results obtained. Here, we describe the technique of left ventricular catheterization using the Scisense conductance catheter system by Transonic; however, the basic method applies to all murine catheter systems. We furthermore indicate possible pitfalls during the procedure and how to avoid them.作者: 蔓藤圖飾 時(shí)間: 2025-3-28 08:15
https://doi.org/10.1007/978-1-349-02402-5 been developed in zebrafish, including ventricular resection, genetic ablation, and ventricular cryoinjury. This chapter provides a detailed protocol of zebrafish ventricular cryoinjury and highlights factors and critical steps to be considered when performing this method.作者: lesion 時(shí)間: 2025-3-28 12:09
Left Ventricular Pressure Volume Loop Measurements Using Conductance Catheters to Assess Myocardial bility and reliability of the results obtained. Here, we describe the technique of left ventricular catheterization using the Scisense conductance catheter system by Transonic; however, the basic method applies to all murine catheter systems. We furthermore indicate possible pitfalls during the procedure and how to avoid them.作者: 變化 時(shí)間: 2025-3-28 16:30
Ventricular Cryoinjury as a Model to Study Heart Regeneration in Zebrafish been developed in zebrafish, including ventricular resection, genetic ablation, and ventricular cryoinjury. This chapter provides a detailed protocol of zebrafish ventricular cryoinjury and highlights factors and critical steps to be considered when performing this method.作者: 煩人 時(shí)間: 2025-3-28 22:08 作者: STEER 時(shí)間: 2025-3-29 01:48 作者: Silent-Ischemia 時(shí)間: 2025-3-29 06:07 作者: growth-factor 時(shí)間: 2025-3-29 11:05
A Genetic Cardiomyocyte Ablation Model for the Study of Heart Regeneration in Zebrafish this severe injury, adult zebrafish efficiently regenerate lost cardiomyocytes and reverse heart failure. In this chapter, we describe the methods for inducing genetic cardiomyocyte?ablation in adult zebrafish, assessing cardiomyocyte proliferation, and histologically analyzing regeneration after injury.作者: 箴言 時(shí)間: 2025-3-29 15:23
Cardiac Regeneration978-1-0716-0668-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 墊子 時(shí)間: 2025-3-29 15:46
Kenneth D. Poss,Bernhard KühnIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts作者: Felicitous 時(shí)間: 2025-3-29 20:52
Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/221817.jpg作者: incontinence 時(shí)間: 2025-3-30 01:59 作者: 不給啤 時(shí)間: 2025-3-30 06:26 作者: TIA742 時(shí)間: 2025-3-30 12:07
https://doi.org/10.1007/978-3-319-47042-9sure volume loop recordings greatly enhancing the technique. While a powerful tool, proper execution of the procedure is paramount to ensure reproducibility and reliability of the results obtained. Here, we describe the technique of left ventricular catheterization using the Scisense conductance cat作者: synchronous 時(shí)間: 2025-3-30 13:07 作者: 帶來墨水 時(shí)間: 2025-3-30 18:02
https://doi.org/10.1007/978-1-349-02402-5brafish is an excellent model organism to study the cellular and molecular mechanisms promoting heart regeneration. Several cardiac injury models have been developed in zebrafish, including ventricular resection, genetic ablation, and ventricular cryoinjury. This chapter provides a detailed protocol作者: Engulf 時(shí)間: 2025-3-31 00:03 作者: 1FAWN 時(shí)間: 2025-3-31 04:14 作者: Hyperlipidemia 時(shí)間: 2025-3-31 07:29
Norman Mailer Quick-Change Artistology and mechanisms of cardiovascular diseases and accelerated the development of therapeutic strategies. It is vitally important to be able to evaluate the therapeutic efficacy and have reliable surrogate markers for therapeutic development for cardiac regeneration research. Magnetic resonance ima作者: 尊嚴(yán) 時(shí)間: 2025-3-31 10:33 作者: deceive 時(shí)間: 2025-3-31 13:53 作者: 彎彎曲曲 時(shí)間: 2025-3-31 19:40 作者: 哀悼 時(shí)間: 2025-4-1 00:30
https://doi.org/10.1057/9780230109056f which are associated with tremendous loss of functional cardiomyocytes and simultaneous formation of scar tissue. Burgeoning studies have shown that the introduction of three minimal transcriptional factors, Gata4, Mef2c, and Tbx5 (G/M/T), could convert murine fibroblasts into iCMs that closely re作者: COMA 時(shí)間: 2025-4-1 02:17
