作者: Anemia 時(shí)間: 2025-3-21 22:40 作者: frugal 時(shí)間: 2025-3-22 02:29
Jos Dute,Michael G. Faure,Helmut Koziolth CRISPR/Cas9 gene editing, to discreetly label genes for live cell imaging to study chromatin dynamics. Our approach improves on some of the fundamental shortfalls associated with current labelling strategies and has the potential for multiplexed observations.作者: Neuralgia 時(shí)間: 2025-3-22 05:13 作者: 地牢 時(shí)間: 2025-3-22 09:53
Single-Cell DamID to Capture Contacts Between DNA and the Nuclear Lamina in Individual Mammalian Cena protein lamin B1, genomic regions in contact with the nuclear periphery can be mapped. Single-cell DamID generates contact maps with sufficient throughput and resolution to reliably identify patterns of similarity as well as variation in nuclear organization of interphase chromosomes.作者: BLANK 時(shí)間: 2025-3-22 16:23
Visualization of Chromatin Dynamics by Live Cell Microscopy Using CRISPR/Cas9 Gene Editing and ANCHth CRISPR/Cas9 gene editing, to discreetly label genes for live cell imaging to study chromatin dynamics. Our approach improves on some of the fundamental shortfalls associated with current labelling strategies and has the potential for multiplexed observations.作者: BLANK 時(shí)間: 2025-3-22 19:59
1064-3745 expertsThis detailed book collects methods based on the evolution of the chromosome conformation capture (3C) technique and other complementary approaches to dissect chromatin conformation with an emphasis on dissection of nuclear compartmentalization and visualization in imaging. Written for the h作者: 抗原 時(shí)間: 2025-3-22 23:56 作者: Carminative 時(shí)間: 2025-3-23 01:56 作者: 直言不諱 時(shí)間: 2025-3-23 08:32 作者: 別炫耀 時(shí)間: 2025-3-23 10:16 作者: 縱欲 時(shí)間: 2025-3-23 13:55
C-HiC: A High-Resolution Method for Unbiased Chromatin Conformation Capture Targeting Small Locus,roach to a capture probe-based version named C-HiC. This updated assay has resulted in an improved throughput analysis, reduced input material, and good repeatability. The protocol described below details our procedure and notes for a C-HiC approach, designed to target only specific portion of a given genome.作者: 過(guò)多 時(shí)間: 2025-3-23 21:41 作者: syncope 時(shí)間: 2025-3-24 01:53 作者: Euthyroid 時(shí)間: 2025-3-24 03:14
https://doi.org/10.5822/978-1-59726-377-1en devoted to the study of the three-dimensional architecture of the genome and its functional implications. In this chapter, I will describe the chromosome conformation capture (3C), one of the first techniques used to detect and measure the frequency of interactions between genomic sequences that are kept in spatial proximity in the nucleus.作者: 辯論 時(shí)間: 2025-3-24 07:00
The Chromosome Conformation Capture (3C) in ,en devoted to the study of the three-dimensional architecture of the genome and its functional implications. In this chapter, I will describe the chromosome conformation capture (3C), one of the first techniques used to detect and measure the frequency of interactions between genomic sequences that are kept in spatial proximity in the nucleus.作者: Exclaim 時(shí)間: 2025-3-24 13:34
Space Geometry from Ancient Greece to Todayroach to a capture probe-based version named C-HiC. This updated assay has resulted in an improved throughput analysis, reduced input material, and good repeatability. The protocol described below details our procedure and notes for a C-HiC approach, designed to target only specific portion of a given genome.作者: 中止 時(shí)間: 2025-3-24 16:25
Best Practices im No-Line-Handel, interactors, such as proteins or DNA sequences. Common challenges faced by this methodology such as cell-type specific permeabilization, target accessibility, image acquisition, and post-acquisition analyses are also discussed.作者: 兩種語(yǔ)言 時(shí)間: 2025-3-24 22:29 作者: squander 時(shí)間: 2025-3-25 01:25
The Chromosome Conformation Capture (3C) in ,tched linear molecule. After that, a huge advance in the knowledge of the structure of the eukaryotic genome in the nuclear space has been made over the last decades, bringing us to the widely accepted concept that the genome is packaged into hierarchical levels of higher-order three-dimensional str作者: 迎合 時(shí)間: 2025-3-25 04:43
4C-Seq: Interrogating Chromatin Looping with Circular Chromosome Conformation Capture,croscopic methods. Among the method derivatives, 4C-seq (circular chromosome conformation capture, coupled to high-throughput sequencing) is a versatile, cost-effective means of assessing all chromatin interactions with a specific genomic region of interest, making it particularly suitable for inter作者: 取消 時(shí)間: 2025-3-25 07:49
Analysis, Modeling, and Visualization of Chromosome Conformation Capture Experiments, in topologically associating domains (TADs), and site-specific looping interactions. The discovery of this genome hierarchical organization emerged from the computational analysis of chromatin capture data. With the increasing availability of such data, automatic pipelines for the robust comparison作者: Chagrin 時(shí)間: 2025-3-25 15:10 作者: Coronary 時(shí)間: 2025-3-25 18:27 作者: 珍奇 時(shí)間: 2025-3-25 23:25 作者: Inclement 時(shí)間: 2025-3-26 03:23 作者: patella 時(shí)間: 2025-3-26 04:45
Single-Cell DamID to Capture Contacts Between DNA and the Nuclear Lamina in Individual Mammalian Cege. To comprehend cell-to-cell variation within a complex system, systematic analysis of individual cells is necessary. While many tools exist to capture DNA conformation and chromatin context, these methods generally require large populations of cells for sufficient output. Here we describe single-作者: jettison 時(shí)間: 2025-3-26 11:09 作者: 把…比做 時(shí)間: 2025-3-26 13:47 作者: Malleable 時(shí)間: 2025-3-26 18:39 作者: nonplus 時(shí)間: 2025-3-26 21:53 作者: debase 時(shí)間: 2025-3-27 03:33 作者: NAIVE 時(shí)間: 2025-3-27 05:27 作者: 注意 時(shí)間: 2025-3-27 09:27
,3D COMBO chrRNA–DNA–ImmunoFISH,ured via the concerted action of different players (transcription factors, “reader” and “writer” enzymes, histone marks, structural proteins, noncoding regulatory RNAs) that flow in the 3D organization of the genome. Indeed, nuclear architecture participates in the punctual and cell-type-specific re作者: 畏縮 時(shí)間: 2025-3-27 15:05 作者: Feckless 時(shí)間: 2025-3-27 18:43
Targeted DNase Hi-C,g phenotype-associated chromatin 3D signatures. Here, I describe a detailed protocol of targeted DNase Hi-C library preparation, which covers experimental steps starting from cell cross-linking to library amplification.作者: eczema 時(shí)間: 2025-3-28 01:45 作者: ALERT 時(shí)間: 2025-3-28 05:13 作者: 牌帶來(lái) 時(shí)間: 2025-3-28 06:47
Formaldehyde-Mediated Snapshot of Nuclear Architecture,hogens..Experimental treatments often require the isolation of cells from their physiological environment, exposing them to possible sudden changes in their nuclear architecture. Here we propose an early cross-linking on primary cells, a fixing method that can help to minimize the risk of nuclear st作者: OTTER 時(shí)間: 2025-3-28 11:15 作者: 火光在搖曳 時(shí)間: 2025-3-28 16:04 作者: 六個(gè)才偏離 時(shí)間: 2025-3-28 21:49 作者: 披肩 時(shí)間: 2025-3-29 00:00 作者: ABHOR 時(shí)間: 2025-3-29 04:49
Space Geometry from Ancient Greece to Todayg phenotype-associated chromatin 3D signatures. Here, I describe a detailed protocol of targeted DNase Hi-C library preparation, which covers experimental steps starting from cell cross-linking to library amplification.作者: 土產(chǎn) 時(shí)間: 2025-3-29 07:40 作者: 曲解 時(shí)間: 2025-3-29 14:48
,Pilze, Lebenspartner der Waldb?ume,n-mediated amplification (LMA) followed by deep sequencing to quantitatively detect specified genomic regions in ChIP samples. Here, we describe the generation of 2C-ChIP libraries and computational processing of the resulting sequencing data.作者: 假裝是你 時(shí)間: 2025-3-29 17:39
https://doi.org/10.1007/978-1-4302-3991-8hogens..Experimental treatments often require the isolation of cells from their physiological environment, exposing them to possible sudden changes in their nuclear architecture. Here we propose an early cross-linking on primary cells, a fixing method that can help to minimize the risk of nuclear st作者: 小樣他閑聊 時(shí)間: 2025-3-29 21:16 作者: 羊欄 時(shí)間: 2025-3-30 03:16
Best Practices im No-Line-Handel,muno-DNA FISH detection of genes and Human immunodeficiency virus 1 (HIV-1) provirus in primary CD4. T cells from healthy donors, or cells infected in vitro with the virus. Our multicolor 3D-FISH technique allows, by using up to three fluorophores, visualization of spatial positioning of loci inside作者: Veneer 時(shí)間: 2025-3-30 06:40
https://doi.org/10.1007/978-1-4684-1467-7imary T cells. We believe that our protocol will contribute to the improvement of epigenetic studies on the 3D nuclear structure of T cell subsets, possibly shedding light on the still hidden epigenetic players responsible for the great plasticity and functional diversification exerted by T cells.作者: 新義 時(shí)間: 2025-3-30 09:50
https://doi.org/10.5822/978-1-59726-377-1tched linear molecule. After that, a huge advance in the knowledge of the structure of the eukaryotic genome in the nuclear space has been made over the last decades, bringing us to the widely accepted concept that the genome is packaged into hierarchical levels of higher-order three-dimensional str作者: 運(yùn)動(dòng)性 時(shí)間: 2025-3-30 15:04
https://doi.org/10.1007/978-3-319-21680-5croscopic methods. Among the method derivatives, 4C-seq (circular chromosome conformation capture, coupled to high-throughput sequencing) is a versatile, cost-effective means of assessing all chromatin interactions with a specific genomic region of interest, making it particularly suitable for inter作者: 陰謀小團(tuán)體 時(shí)間: 2025-3-30 16:58
Space Geometry from Ancient Greece to Today in topologically associating domains (TADs), and site-specific looping interactions. The discovery of this genome hierarchical organization emerged from the computational analysis of chromatin capture data. With the increasing availability of such data, automatic pipelines for the robust comparison作者: 額外的事 時(shí)間: 2025-3-30 21:59 作者: 儲(chǔ)備 時(shí)間: 2025-3-31 04:01
Space Geometry from Ancient Greece to Todaylar approaches has unraveled the existence of DNA territories. With the emergence of affordable deep-sequencing approaches, “Cs” techniques such as 4C, 5C, and HiC, to name a few, are now routinely performed by the scientific community in a large number of model systems. We have modified the HiC app作者: 讓步 時(shí)間: 2025-3-31 07:31
,Pilze, Lebenspartner der Waldb?ume,t diverse scale lengths with chromosomes occupying distinct volumes and individual chromosomes folding into compartments, inside which the chromatin fiber is packed in large domains (as the topologically associating domains, TADs) and forms short-range interactions (as enhancer-promoter loops). The 作者: lymphoma 時(shí)間: 2025-3-31 12:10
,Pilze, Lebenspartner der Waldb?ume,d is often used alongside chromosome conformation capture approaches to obtain a better-rounded view of the functional relationship between chromatin architecture and its landscape. Since the inception of ChIP, its protocol has been modified to improve speed, sensitivity, and specificity. Combining 作者: JAMB 時(shí)間: 2025-3-31 15:28 作者: 笨拙的我 時(shí)間: 2025-3-31 20:31 作者: 琺瑯 時(shí)間: 2025-3-31 22:27 作者: Obliterate 時(shí)間: 2025-4-1 05:40
Compensation in the Swiss Health Care Sector. Here we propose a methodology that combines the use of micro-patterning of adhesive molecules to impose single-cell geometry, with visualization of chromosome territories. This allows obtaining a representative statistical map of the absolute positions of chromosome territories relative to the geo
