作者: 粗魯性質(zhì) 時(shí)間: 2025-3-21 20:57
,Capillary Electrophoresis–Mass Spectrometry (CE-MS) by Sheath–Flow Nanospray Interface and Its Use cterization of biologics in the biopharmaceutical industry. The direct coupling of CE with MS is an attractive approach, in that the high separation capability of CE and the ultrasensitive detection and accurate identification performance of MS can be combined to provide a powerful system for the an作者: exhilaration 時(shí)間: 2025-3-22 03:45 作者: 綁架 時(shí)間: 2025-3-22 07:44 作者: Canary 時(shí)間: 2025-3-22 09:29
Successive Multiple Ionic-Polymer Layer Coatings for Intact Protein Analysis by Capillary Zone Elecften are applied to prevent adsorption and to determine the electroosmotic flow (EOF), which is of major importance for the separation in CE. Successive multiple ionic-polymer layer (SMIL) coatings are frequently used for protein analysis in capillary electrophoresis resulting in high separation eff作者: 枯萎將要 時(shí)間: 2025-3-22 14:22 作者: 枯萎將要 時(shí)間: 2025-3-22 17:51
Bottom-Up Analysis of Proteins by Peptide Mass Fingerprinting with tCITP-CZE-ESI-TOF MS After Trypttically digested, mostly by trypsin, and the resulting peptides are then separated prior to their transfer to a mass spectrometer. The following protocol portrays a bottom-up method, which was optimized for the application of CZE-ESI-TOF MS. Protein denaturation is achieved by addition of 2,2,2-trif作者: Jogging 時(shí)間: 2025-3-22 23:32 作者: 金哥占卜者 時(shí)間: 2025-3-23 04:01
Assessment of Macro- and Microheterogeneity of Monoclonal Antibodies Using Capillary Zone Electrophlonal antibodies (mAbs). mAbs are complex molecules comprising different glycoforms and many other posttranslational modifications. In addition to this inherent microheterogeneity, misassembling of antibodies can take place during production contributing to their macroheterogeneity. CZE-MS is a vers作者: 冷淡周邊 時(shí)間: 2025-3-23 08:43
High-Sensitivity Glycoproteomic Analysis of Biological Samples by CZE-ESI-MS,icroheterogeneity of a glycoprotein in a bottom-up approach. This chapter describes in detail the sample preparation procedures using a purified biological sample, prostate-specific antigen, as a model protein, including proteolytic digestion (trypsin). In addition, insights are provided into the st作者: Intruder 時(shí)間: 2025-3-23 13:23 作者: orthopedist 時(shí)間: 2025-3-23 15:00 作者: modish 時(shí)間: 2025-3-23 18:48 作者: 向宇宙 時(shí)間: 2025-3-24 00:58
Applications of Capillary Electrophoresis-Mass Spectrometry to Chiral Analysis, spectrometry (MS) detection further boosts the possibilities of CE in terms of achieving higher sensitivity with the possibility of an unambiguous assignment of analytes of interest. This chapter details four different examples using CE in the electrokinetic chromatography (EKC) mode coupled to MS 作者: 有害 時(shí)間: 2025-3-24 02:23
Improved Sensitivity to Determine Antibiotic Residues in Chicken Meat by In-Line Solid-Phase Extractration sensitivity has been not high enough, mainly in relation to the monitoring of contaminants and residues, such as pesticides, veterinary medicines, environmental contaminants, toxins, etc. For this reason, researchers have proposed several strategies to overcome this limitation. So far, appro作者: 的闡明 時(shí)間: 2025-3-24 07:19
CE Coupled to ICP-MS and Single Particle ICP-MS for Nanoparticle Analysis,ICP-MS) or single particle (sp)-ICP-MS enhances the analytical performance and capabilities of the method compared to CE with a standard detector (ultraviolet visible spectroscopy), in particular for trace analysis of metals or metal-containing compounds. spICP-MS is a method for NP analysis, where 作者: 食物 時(shí)間: 2025-3-24 13:21 作者: harpsichord 時(shí)間: 2025-3-24 16:53 作者: Magnitude 時(shí)間: 2025-3-24 21:46 作者: hemoglobin 時(shí)間: 2025-3-24 23:33 作者: 討厭 時(shí)間: 2025-3-25 05:38
Successive Multiple Ionic-Polymer Layer Coatings for Intact Protein Analysis by Capillary Zone Elec containing 2?M acetic acid. DEAEDq-PMA coating was found to be the most suitable for hemoglobin analysis due to relatively low reversed electroosmotic mobility leading to increased electrophoretic resolution of closely related proteoforms. Thereby, not only alpha and beta subunit of the hemoglobin 作者: 縱欲 時(shí)間: 2025-3-25 08:32
Bottom-Up Analysis of Proteins by Peptide Mass Fingerprinting with tCITP-CZE-ESI-TOF MS After TryptHyphenation to a time-of-flight (TOF) mass spectrometer is carried out by a triple-tube coaxial sheath flow interface, which uses electrospray ionization (ESI). Peptide identification is done by peptide mass fingerprinting (PMF). The protocol is outlined exemplarily for a model protein, i.e., bovine作者: Affirm 時(shí)間: 2025-3-25 12:46 作者: 印第安人 時(shí)間: 2025-3-25 17:45
,Capillary Zone Electrophoresis–Electrospray Ionization Tandem Mass Spectrometry for Total Analysis ination contributed an essential progress to this field..In the present chapter, two powerful methods based on CZE for separation and ESI-MS for identification and structural analysis of CS/DS are presented. The first part is devoted to offline CZE-ESI-MS based on fraction collection, screening by n作者: 打擊 時(shí)間: 2025-3-25 20:41
Book 2022 introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols...?..Authoritative and cutting-edge,?.Capillary Electrophoresis-Mass Spectrometry: Methods and Protocols .aims to provide highly v作者: 任意 時(shí)間: 2025-3-26 01:56
1064-3745 and step-by-step, readily reproducible protocols...?..Authoritative and cutting-edge,?.Capillary Electrophoresis-Mass Spectrometry: Methods and Protocols .aims to provide highly v978-1-0716-2495-1978-1-0716-2493-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Cabg318 時(shí)間: 2025-3-26 08:04 作者: AMPLE 時(shí)間: 2025-3-26 10:21 作者: synovitis 時(shí)間: 2025-3-26 13:39 作者: 滔滔不絕地講 時(shí)間: 2025-3-26 20:42
Robert M. Bowers,Janey Lee,Tanja Woykerengths of using capillary electrophoresis for obtaining isomer separation of differently linked sialic acids. Lastly, approaches and potential pitfalls for the integration and quantitation of glycopeptide signals from the obtained CZE-MS data are discussed.作者: excrete 時(shí)間: 2025-3-26 23:58
1064-3745 ation advice from the experts.This volume details aspects and applications of interfacing capillary electrophoresis (CE) with mass spectrometry (MS). Chapters guide readers through approaches based on different types of CE-MS interfaces such as (nano)sheath liquid, porous tip, and liquid junction, a作者: Lumbar-Spine 時(shí)間: 2025-3-27 01:34 作者: amenity 時(shí)間: 2025-3-27 05:44
History of DNA Sequencing Technologiesr, we describe in detail the strategy for the targeted screening of modified amino acids (MAAs), using as an example .-proline and its modified derivatives. We illustrate the strategy with two case studies in human plasma.作者: cardiovascular 時(shí)間: 2025-3-27 13:28 作者: 不可接觸 時(shí)間: 2025-3-27 16:36
Top-Down Proteomics by Capillary Zone Electrophoresis-Tandem Mass Spectrometry for Large-Scale Charpillaries, a dynamic pH junction sample stacking method, a commercialized electro-kinetically pumped sheath flow CE-MS interface and an Orbitrap mass spectrometer. CZE-MS/MS can identify hundreds of proteoforms routinely from the . sample with a 1% proteoform-level false discovery rate (FDR).作者: 天賦 時(shí)間: 2025-3-27 20:10 作者: carbohydrate 時(shí)間: 2025-3-28 00:48
,Assessing the Energy Status of Low Numbers of Mammalian Cells by Capillary Electrophoresis–Mass Speng with cell cultures is the adenylate energy charge (AEC), which represents an index of the energy state of a cell. In this protocol, a CE-MS strategy is proposed for the reliable determination of the adenylate energy charge (AEC) in metabolomics studies dealing with low numbers of mammalian cells.作者: avulsion 時(shí)間: 2025-3-28 05:06
,Protocol for Etching Bare-Fused Silica Capillaries for Sheathless Capillary Electrophoresis–Mass Sption of a suitable capillary for the porous tip sprayer is more challenging. The porous tip is created by etching the bare-fused silica capillary with hydrofluoric acid. Here we describe the complete process of etching bare-fused silica capillaries, from length cutting to quality control of the newly etched capillary.作者: Absenteeism 時(shí)間: 2025-3-28 06:38 作者: Infantry 時(shí)間: 2025-3-28 11:46 作者: 未開化 時(shí)間: 2025-3-28 14:38 作者: institute 時(shí)間: 2025-3-28 21:17
Book 2022h approaches based on different types of CE-MS interfaces such as (nano)sheath liquid, porous tip, and liquid junction, as well as various capillary coatings, and a broad range of applications including several top-down and bottom-up proteomic approaches. Additionally, a list of analyte targets was 作者: 不整齊 時(shí)間: 2025-3-28 22:54
Hagen Woesner,Martin Maier,Adam Woliszn parameters of a new CE-MS interface design, assess its analytical performance, and compare it to a reference interface if desired. Electrospray stability, sensitivity, reproducibility, and robustness are practically evaluated as key elements of the process.作者: gonioscopy 時(shí)間: 2025-3-29 06:12
Hagen Woesner,Martin Maier,Adam Woliszn a single-dose pharmacokinetic mice study. Optimization processes, including the finetuning of CE conditions and MS parameters, are illustrated in this chapter, with focuses on method robustness and assay reproducibility.作者: gangrene 時(shí)間: 2025-3-29 11:03
https://doi.org/10.1007/978-1-4614-7001-4nation. In the last two examples, PFT was not needed as they were based on the use of low concentrations of chiral selector but a derivatization step was necessary to allow proper enantiorecognition with the chiral selectors. Description of these protocols is fully explained.作者: TOM 時(shí)間: 2025-3-29 12:50 作者: insular 時(shí)間: 2025-3-29 18:27
Victor Wei Zhang,Lee-Jun C. Wongoncentration. CE-spICP-MS allows obtaining the average size, size distribution, elemental composition, and particle number concentration (PNC) of NPs in addition to a CE separation profile in a single run. Moreover, CE-spICP-MS can be used in some cases to separate NPs with different coatings.作者: 斥責(zé) 時(shí)間: 2025-3-29 21:40 作者: GROUP 時(shí)間: 2025-3-30 02:46
,Capillary Electrophoresis–Mass Spectrometry (CE-MS) by Sheath–Flow Nanospray Interface and Its Use n a single-dose pharmacokinetic mice study. Optimization processes, including the finetuning of CE conditions and MS parameters, are illustrated in this chapter, with focuses on method robustness and assay reproducibility.作者: infelicitous 時(shí)間: 2025-3-30 06:14
Applications of Capillary Electrophoresis-Mass Spectrometry to Chiral Analysis,nation. In the last two examples, PFT was not needed as they were based on the use of low concentrations of chiral selector but a derivatization step was necessary to allow proper enantiorecognition with the chiral selectors. Description of these protocols is fully explained.作者: 土產(chǎn) 時(shí)間: 2025-3-30 09:37
Improved Sensitivity to Determine Antibiotic Residues in Chicken Meat by In-Line Solid-Phase Extrache analytes before separation takes place. In this chapter, this strategy is applied to CE coupled to tandem mass spectrometry (MS/MS) for the multiresidue detection of quinolone antibiotic residues in chicken meat. A previous sample treatment based on pressurized liquid extraction to obtain an optimum performance is also described.作者: 無(wú)王時(shí)期, 時(shí)間: 2025-3-30 15:30
CE Coupled to ICP-MS and Single Particle ICP-MS for Nanoparticle Analysis,oncentration. CE-spICP-MS allows obtaining the average size, size distribution, elemental composition, and particle number concentration (PNC) of NPs in addition to a CE separation profile in a single run. Moreover, CE-spICP-MS can be used in some cases to separate NPs with different coatings.作者: 苦惱 時(shí)間: 2025-3-30 17:12 作者: decode 時(shí)間: 2025-3-30 21:56 作者: 的闡明 時(shí)間: 2025-3-31 02:46 作者: disciplined 時(shí)間: 2025-3-31 05:41 作者: 很是迷惑 時(shí)間: 2025-3-31 10:08
