作者: vitrectomy 時(shí)間: 2025-3-21 20:27 作者: Fester 時(shí)間: 2025-3-22 00:59 作者: Digest 時(shí)間: 2025-3-22 05:01 作者: Feigned 時(shí)間: 2025-3-22 09:31
Quantitation Procedures a plot of UV absorbance vs time. Therefore, HPLC data handling and peak integration packages are generally applicable to CE. Main component assay may be performed using external and internal standardization or by standard addition. Impurity data can be calculated and reported as either % w/w or % a作者: Perceive 時(shí)間: 2025-3-22 14:52 作者: Perceive 時(shí)間: 2025-3-22 17:47 作者: 無動(dòng)于衷 時(shí)間: 2025-3-22 22:13
Method ValidationThe extent of tests applied will vary according to the nature of the application and the extent of intended usage. Generally, such aspects as accuracy, precision, reproducibility, linearity, robustness, and method of transfer are evaluated during validation.作者: evasive 時(shí)間: 2025-3-23 05:06 作者: aviator 時(shí)間: 2025-3-23 08:45 作者: 噴出 時(shí)間: 2025-3-23 10:43
Capillary Gel Electrophoresisein and complex carbohydrate molecules (.,.). For relatively short single-stranded DNA units (i.e., oligonucleotides) and carbohydrate molecules, there is a need to separate by a single base difference (for DNA sequencing) (.) or even for identical chain length with a different sequence (identificat作者: 謙卑 時(shí)間: 2025-3-23 15:41
Chiral Separations by Capillary Electrophoresisns have been achieved in Free Solution Capillary Electrophoresis (FSCE) employing cyclodextrins (CDs) as chiral selectors. This is because of their ready availability and broad applicability. Chiral resolutions have also been achieved in micellar electrokinetic chromatography (MEKC) through the use 作者: 出沒 時(shí)間: 2025-3-23 19:39
Capillary Electrochromatographyf a high-pressure mechanical pump. However, under certain conditions, the mobile phase can be forced to migrate through the chromatographic medium by the application of an electric field. This phenomenon of electrically induced fluid flow is commonly referred to as electro-osmosis or electro-osmotic作者: Loathe 時(shí)間: 2025-3-23 22:47 作者: 行為 時(shí)間: 2025-3-24 05:38
Analysis of Bases, Nucleosides, and (Oligo)nucleotides by Capillary Electrophoresislogy associated with capillary gel electrophoresis (CGE) is covered elsewhere in this book (.) and in recent reviews (.–.). A number of nucleosides and nucleotides have been used in chemotherapy as antiviral agents, e.g., 3′-azido-3′-deoxythymidine (azT), and 2′,3′-dideoxyinosine (ddI), and 2′,3′-di作者: B-cell 時(shí)間: 2025-3-24 06:51
Application of Capillary Electrophoresis to Pharmaceutical Analysisrug may be analyzed in its anionic form at high pH, and basic drugs may be tested at low pH in their cationic form. . shows the efficient separation of a test mixture containing 11 basic drugs using a simple low-pH electrolyte. Zwitterionic drugs (those containing both acidic and basic groups) may b作者: 能量守恒 時(shí)間: 2025-3-24 13:35
Separation of Peptides and Protein Digests by Capillary Electrophoresisences between peptides (.,.). Recently, however, capillary electrophoresis (CE) has increasingly been used for peptide analysis. In capillary zone electrophoresis (CZE), the separation mechanism is based mainly on differences in charge-to-mass ratios, and since peptides are amphoteric, they are idea作者: Agility 時(shí)間: 2025-3-24 17:32 作者: 取之不竭 時(shí)間: 2025-3-24 19:47 作者: 轉(zhuǎn)向 時(shí)間: 2025-3-25 02:05
The Industrial Context of Newspaper Firms, a plot of UV absorbance vs time. Therefore, HPLC data handling and peak integration packages are generally applicable to CE. Main component assay may be performed using external and internal standardization or by standard addition. Impurity data can be calculated and reported as either % w/w or % area/area.作者: LIMIT 時(shí)間: 2025-3-25 03:37 作者: cumulative 時(shí)間: 2025-3-25 07:47
Least Squares Problems: Gauss-Newton MethodsThe extent of tests applied will vary according to the nature of the application and the extent of intended usage. Generally, such aspects as accuracy, precision, reproducibility, linearity, robustness, and method of transfer are evaluated during validation.作者: Legend 時(shí)間: 2025-3-25 15:43
https://doi.org/10.1057/978-1-137-54109-3is (CE). This is not an exhaustive treatment, but is considered sufficient to comprehend and appreciate the principles of CE. More detailed theoretical background can be obtained from a number of reference books (.–.). Developments in the field of CE are reviewed in detail annually in the journal ..作者: harbinger 時(shí)間: 2025-3-25 16:28
https://doi.org/10.1007/978-3-030-73624-8d the relative performance and features of these commercial systems (.,.). Systems are available in both single bench-top units or in modular form. . shows a photograph of a commercial unit. Currently the CE equipment market is dominated by the major HPLC instrument supply companies, such as Beckman作者: 外表讀作 時(shí)間: 2025-3-25 20:32
https://doi.org/10.1007/978-3-030-73624-8l positions, injection parameters, and rinse cycles, are defined in the steps within the method. A typical method is given below. The exact settings for the parameters are determined during method development. A method can be run for a single sample. To analyze several samples, a sequence is created作者: 憤怒事實(shí) 時(shí)間: 2025-3-26 01:47 作者: Rheumatologist 時(shí)間: 2025-3-26 07:49
The Industrial Context of Newspaper Firms, a plot of UV absorbance vs time. Therefore, HPLC data handling and peak integration packages are generally applicable to CE. Main component assay may be performed using external and internal standardization or by standard addition. Impurity data can be calculated and reported as either % w/w or % a作者: facetious 時(shí)間: 2025-3-26 11:47
Systems of Equations: Global Newton Methodse expected. The advent of reliable commercial instruments and improved methodology can now enable RSD figures of 1–2% or better to be routinely obtained (.). The following chapter provides some guidelines toward optimizing precision.作者: lethargy 時(shí)間: 2025-3-26 13:45
Systems of Equations: Global Newton Methods be only 50 × 200 μm. Both these factors influence the detector sensitivity to a large extent. CE is less sensitive when directly compared to HPLC with typical injections of 10–50 μL and 1-cm detector cells. The difference may be up to an order of magnitude (.) when comparing at the same UV waveleng作者: 中止 時(shí)間: 2025-3-26 20:32 作者: 評論性 時(shí)間: 2025-3-27 00:07
Springer Series in Computational Mathematics fraction collection. Even using optimized conditions, the amounts collected employing preparative CE are extremely small and are on the order of nanogram quantities. However, these levels can be useful to perform operations such as: (1) peak purity verification by reinjection on CE or onto another 作者: micronutrients 時(shí)間: 2025-3-27 04:30
https://doi.org/10.1007/978-94-017-2426-5d. The technique was first introduced by Mikkers et al. (.), Jorgenson and Lukacs (.), and Hjertén (.), as an instrumental version of electrophoresis. Although CE can generally give a higher resolution within a shorter time compared with conventional high-performance liquid chromatography (HPLC), on作者: 無思維能力 時(shí)間: 2025-3-27 06:02 作者: 門閂 時(shí)間: 2025-3-27 11:51 作者: 向前變橢圓 時(shí)間: 2025-3-27 15:49 作者: declamation 時(shí)間: 2025-3-27 21:16 作者: 襲擊 時(shí)間: 2025-3-28 01:57 作者: 涂掉 時(shí)間: 2025-3-28 03:06
https://doi.org/10.1007/b101082rug may be analyzed in its anionic form at high pH, and basic drugs may be tested at low pH in their cationic form. . shows the efficient separation of a test mixture containing 11 basic drugs using a simple low-pH electrolyte. Zwitterionic drugs (those containing both acidic and basic groups) may b作者: Palpate 時(shí)間: 2025-3-28 08:02
Introduction and Basic Concepts,ences between peptides (.,.). Recently, however, capillary electrophoresis (CE) has increasingly been used for peptide analysis. In capillary zone electrophoresis (CZE), the separation mechanism is based mainly on differences in charge-to-mass ratios, and since peptides are amphoteric, they are idea作者: 內(nèi)行 時(shí)間: 2025-3-28 14:02 作者: Inscrutable 時(shí)間: 2025-3-28 14:37 作者: 影響 時(shí)間: 2025-3-28 18:44 作者: addition 時(shí)間: 2025-3-29 02:16
Quick Guide to Running a Successful SeparationThis chapter is intended to act as a brief guide to the operations required to perform a series of CE analyses and subsequent care of the capillary.作者: transient-pain 時(shí)間: 2025-3-29 07:01
Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/221389.jpg作者: 鎮(zhèn)壓 時(shí)間: 2025-3-29 07:45
Method Development/Optimizationstarting points and guidelines for method development of chiral separations. A more extensive treatment of chiral separations is given in .. A detailed background to the parameters affecting MECC separations is given in ..作者: NEG 時(shí)間: 2025-3-29 12:48
Quantitation Procedures a plot of UV absorbance vs time. Therefore, HPLC data handling and peak integration packages are generally applicable to CE. Main component assay may be performed using external and internal standardization or by standard addition. Impurity data can be calculated and reported as either % w/w or % area/area.作者: 遭遇 時(shí)間: 2025-3-29 17:23 作者: 招人嫉妒 時(shí)間: 2025-3-29 22:42 作者: JEER 時(shí)間: 2025-3-30 02:42
Newton and the Rosicrucian Enlightenment,l-defined pore size. Noncrosslinked, or so-called physical gels, have a dynamic pore structure. This major difference provides the noncrosslinked linear polymer networks with much higher flexibility when compared to the crosslinked gels. One can operate at high temperatures (up to 50–70°C) while app作者: 比目魚 時(shí)間: 2025-3-30 05:53
https://doi.org/10.1007/978-1-4684-0553-8,.). However, the first discussion since the advent of modern HPLC, proposing the use of electro-osmotic flow as an alternative to pressure driven flow was given by Pretorious and coworkers in 1974 (.).作者: 獸皮 時(shí)間: 2025-3-30 10:02 作者: degradation 時(shí)間: 2025-3-30 13:04 作者: Gum-Disease 時(shí)間: 2025-3-30 17:29 作者: Projection 時(shí)間: 2025-3-31 00:38 作者: Cardioversion 時(shí)間: 2025-3-31 03:14 作者: 津貼 時(shí)間: 2025-3-31 05:52 作者: 向宇宙 時(shí)間: 2025-3-31 11:55
Analysis of Bases, Nucleosides, and (Oligo)nucleotides by Capillary Electrophoresisd PCR, DNA fingerprinting, and antisense therapeutic reagents. Traditionally, chromatographic methods (ion-exchange-, ion-pairing, and re versed-phase LC) or enzymatic methods have been used for purification and purity analysis of synthetic oligonucleotides (.); classical electrophoretic methods are作者: hereditary 時(shí)間: 2025-3-31 16:40 作者: Ligneous 時(shí)間: 2025-3-31 19:58 作者: single 時(shí)間: 2025-3-31 22:37
Separation of Peptides and Protein Digests by Capillary Electrophoresishe provision of such a complementary separation mechanism is extremely useful in characterization of complex biological samples and, in particular, where purification of peptides and proteins is often done by preparative HPLC, such an orthogonal technique is essential for purity control (.,.).作者: Pander 時(shí)間: 2025-4-1 05:17 作者: growth-factor 時(shí)間: 2025-4-1 08:27 作者: GOAT 時(shí)間: 2025-4-1 13:32
1064-3745 y maturing technique, detailed validated protocols are not widely established. Therefore, extensive experimental procedures are not pro- vided for individual analyses. The intention is to provide general guide- lines on the principles and practice of CE and to give an overview of the specific techno作者: 不妥協(xié) 時(shí)間: 2025-4-1 17:28
https://doi.org/10.1057/978-1-137-54109-3l background can be obtained from a number of reference books (.–.). Developments in the field of CE are reviewed in detail annually in the journal .. For example, the 801 papers published in 1992–1993 were recently reviewed (.).作者: ALB 時(shí)間: 2025-4-1 21:10
https://doi.org/10.1007/978-3-030-73624-8shows a photograph of a commercial unit. Currently the CE equipment market is dominated by the major HPLC instrument supply companies, such as Beckman (Fullerton, CA), Hewlett Packard (Waldbronn, Germany), Perkin Elmer (Foster City, CA), and Waters (Milford, MA).作者: MUTE 時(shí)間: 2025-4-1 23:34
https://doi.org/10.1007/978-3-030-73624-8or the parameters are determined during method development. A method can be run for a single sample. To analyze several samples, a sequence is created in which the method, number of samples, and injection repetitions are defined. A typical method listing is given below. Additional information concerning each process is then described.
