標題: Titlebook: Candida Species; Methods and Protocol Richard Calderone Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under excl [打印本頁] 作者: Limbic-System 時間: 2025-3-21 17:06
書目名稱Candida Species影響因子(影響力)
書目名稱Candida Species影響因子(影響力)學科排名
書目名稱Candida Species網絡公開度
書目名稱Candida Species網絡公開度學科排名
書目名稱Candida Species被引頻次
書目名稱Candida Species被引頻次學科排名
書目名稱Candida Species年度引用
書目名稱Candida Species年度引用學科排名
書目名稱Candida Species讀者反饋
書目名稱Candida Species讀者反饋學科排名
作者: expunge 時間: 2025-3-21 22:46 作者: 現存 時間: 2025-3-22 00:29 作者: 左右連貫 時間: 2025-3-22 05:35
A Proteomic Approach for the Quantification of Posttranslational Protein Lysine Acetylation in stance of . is often regulated by posttranslational modifications (PTM) of proteins via lysine acetylation by lysine acetyltransferases. Here, we report an experimental approach using tandem mass tag (TMT) labeling for the detection and quantification of lysine acetylation of histone and nonhistone proteins in 作者: 脊椎動物 時間: 2025-3-22 12:02 作者: 金絲雀 時間: 2025-3-22 13:01 作者: 金絲雀 時間: 2025-3-22 19:20 作者: 清洗 時間: 2025-3-22 22:14 作者: AMBI 時間: 2025-3-23 03:51
The Techniques Innovated to Support NSE,can aid in bridging our understanding of complex genetic networks, with applications to diverse microbial pathogens to further our understanding of virulence, the use of antimicrobial therapies, and host–pathogen interactions.作者: DAUNT 時間: 2025-3-23 08:50
The Techniques Innovated to Support NSE,a direct link between a phenotypic screen (easy read-out) and the identity of the gene target. Screens using mutant libraries can identify chemical–genetic interactions and genes or pathways affected by compounds to decipher the mechanism of action. Herein, we describe a genetic screen of an anti-. natural product.作者: 迅速飛過 時間: 2025-3-23 10:47 作者: 燈絲 時間: 2025-3-23 16:48 作者: 樂意 時間: 2025-3-23 21:27
The Techniques Innovated to Support NSE,ologic response and resulting symptomatic condition and focuses further on assays used to demonstrate “neutrophil anergy” in the model. These methods may serve as a source or resource for further experimentation with the ultimate goal to reduce or eliminate VVC/RVVC.作者: 自制 時間: 2025-3-23 22:48
Construction of Double Heterozygous Deletion Strains for Complex Haploinsufficiency-Based Genetic An1000783, 2009). We also outline the steps to confirming the genotype of the resulting transformants as well as an example of the use of double heterozygous deletion mutants for complex haploinsufficiency analysis of biofilm formation.作者: Vulnerable 時間: 2025-3-24 03:38
Using Synthetic Genetic Interactions in , as a Novel Method to Detect Genes with Roles in Antifungalcan aid in bridging our understanding of complex genetic networks, with applications to diverse microbial pathogens to further our understanding of virulence, the use of antimicrobial therapies, and host–pathogen interactions.作者: Temporal-Lobe 時間: 2025-3-24 08:05
Genetic Screens of an Anti-, Natural Product Using the Heterozygous , Mutant Librarya direct link between a phenotypic screen (easy read-out) and the identity of the gene target. Screens using mutant libraries can identify chemical–genetic interactions and genes or pathways affected by compounds to decipher the mechanism of action. Herein, we describe a genetic screen of an anti-. natural product.作者: 伙伴 時間: 2025-3-24 13:52 作者: 粗野 時間: 2025-3-24 16:18 作者: instill 時間: 2025-3-24 21:59
Methods Related to the Immunopathogenesis of Vulvovaginal Candidiasis and Associated Neutrophil Anerologic response and resulting symptomatic condition and focuses further on assays used to demonstrate “neutrophil anergy” in the model. These methods may serve as a source or resource for further experimentation with the ultimate goal to reduce or eliminate VVC/RVVC.作者: circuit 時間: 2025-3-25 00:51 作者: Flustered 時間: 2025-3-25 05:11 作者: 種子 時間: 2025-3-25 08:27
Plasmid-Based CRISPR–Cas9 Editing in Multiple , Speciescombination with a repair template for disrupting gene function by insertion of a premature stop codon or by gene deletion. The plasmids are easily lost in the absence of selection, allowing scarless gene editing and minimizing detrimental effects of prolonged Cas9 expression.作者: 尾隨 時間: 2025-3-25 15:11
A Simple Method for Growth of , Biofilms Under Continuous Media Flow and for Recovery of Biofilm Disories. Biofilms developed using this system are robust and particularly suitable for studies requiring large amounts of biofilm (dispersed) cells for downstream analyses. Importantly, this apparatus mimics in vivo flow conditions, thereby making it a physiologically relevant model.作者: 案發(fā)地點 時間: 2025-3-25 16:04 作者: 脖子 時間: 2025-3-25 22:11
The Techniques Innovated to Support NSE,pper and how to browse large-scale datasets using the JBrowse genome browser. Finally, it shows how to search and retrieve data for user-defined sets of genes using the Advanced Search and Batch Download tools.作者: AXIOM 時間: 2025-3-26 00:40 作者: 有惡臭 時間: 2025-3-26 06:59
https://doi.org/10.1007/978-1-4419-7326-9e ever-increasing number of applications to the study of pathogenesis, drug resistance, gene expression, and host pathogen interaction and drug discovery. Here, we describe the use of plasmid-based systems for high efficiency CRISPR-Cas9 gene editing into any strain of four non-. species, namely, .,作者: Prophylaxis 時間: 2025-3-26 08:39 作者: 證實 時間: 2025-3-26 15:07 作者: 脫離 時間: 2025-3-26 17:53 作者: 捐助 時間: 2025-3-26 22:54 作者: 無聊的人 時間: 2025-3-27 01:53 作者: 傳授知識 時間: 2025-3-27 07:37
The Techniques Innovated to Support NSE,ovel candidates. However, both methods have limitations which hinder the rapid identification of physiologically active compounds that act via a defined mechanism of action. The method described herein is an efficient, sensitive, and HTP compatible approach that utilizes the principles of competitiv作者: 博愛家 時間: 2025-3-27 09:42
The Techniques Innovated to Support NSE,ntions..Accurate identification and quantification of lipid species in complex lipid mixtures are crucial for assigning biological functions to lipids of fungi. Recently, much has been achieved in the field of mass spectrometry, allowing high-throughput screening of simple and complex lipid structur作者: 漂泊 時間: 2025-3-27 14:47
The Techniques Innovated to Support NSE, antifungal agents with new pharmacological targets for a more efficient management of . infections. Drug repositioning or drug repurposing has been exploited to develop new antifungal approaches. Natural products may be more easily developed because they have been a useful source of active antimicr作者: 不適當 時間: 2025-3-27 20:32 作者: 親屬 時間: 2025-3-27 21:56 作者: 存在主義 時間: 2025-3-28 03:14
The Techniques Innovated to Support NSE,ding of secondary metabolites or enzyme-guided modification of protein targets can be assessed by quantifying the rate of energy transfer between two adequate fluorophores. The AKAR3 sensor contains a protein kinase A (PKA) phosphorylation site, which upon phosphorylation interacts with a ligand dom作者: Fatten 時間: 2025-3-28 09:00 作者: Innocence 時間: 2025-3-28 13:57
Mingzheng Wang,Xiuzhi Shi,Jian Zhouw of body fluids and subjected to shear stress forces. Cells dispersed from . biofilms are highly virulent and developmentally distinct from their parent biofilms. To study biofilm dispersed cells, it becomes imperative to isolate newly dispersed cells using a flow biofilm model. In this chapter, we作者: 機構 時間: 2025-3-28 17:20
Sustainable Civil Infrastructuresures metabolic activity of sessile cells and can also be easily adapted for antifungal susceptibility testing. The entire procedure takes 2–3?days to complete, reliably quantifies biofilms, and provides reproducible results that are imperative toward the standardization of antifungal susceptibility 作者: SSRIS 時間: 2025-3-28 21:02 作者: 大量 時間: 2025-3-28 23:41 作者: 協定 時間: 2025-3-29 03:08 作者: 茁壯成長 時間: 2025-3-29 09:10
Sustainable Civil Infrastructuresures metabolic activity of sessile cells and can also be easily adapted for antifungal susceptibility testing. The entire procedure takes 2–3?days to complete, reliably quantifies biofilms, and provides reproducible results that are imperative toward the standardization of antifungal susceptibility testing of biofilms.作者: 提升 時間: 2025-3-29 14:37
Richard CalderoneIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts作者: 鋼盔 時間: 2025-3-29 16:13 作者: 問到了燒瓶 時間: 2025-3-29 22:11 作者: 青少年 時間: 2025-3-30 00:25 作者: Jocose 時間: 2025-3-30 07:27 作者: 離開就切除 時間: 2025-3-30 10:45
High-Throughput Phospholipidomics of , Cells: From Sample Preparation to Data Analysislatform, we can achieve rapid, sensitive, and accurate quantification of lipids in . extracts. Moreover, the approach is simple and relatively cost-effective. Below, we discuss the key features of ion trap HPLC-ESI-MS/MS-based comparative lipidomics of . cells.作者: 憤怒歷史 時間: 2025-3-30 14:42 作者: 他日關稅重重 時間: 2025-3-30 16:48 作者: 評論性 時間: 2025-3-30 23:41 作者: Malfunction 時間: 2025-3-31 02:59
The Techniques Innovated to Support NSE,ixed with formaldehyde in whole cells which are then lysed; (2) the fixed chromatin is digested with a carefully chosen restriction enzymes to separate intervening DNA fragments; (3) the fragments brought into proximity by DNA looping are ligated in conditions favoring intramolecular ligation; and (
