標(biāo)題: Titlebook: Cancer Genomics and Proteomics; Methods and Protocol Narendra Wajapeyee Book 2014Latest edition Springer Science+Business Media New York 20 [打印本頁] 作者: SPARK 時間: 2025-3-21 17:11
書目名稱Cancer Genomics and Proteomics影響因子(影響力)
書目名稱Cancer Genomics and Proteomics影響因子(影響力)學(xué)科排名
書目名稱Cancer Genomics and Proteomics網(wǎng)絡(luò)公開度
書目名稱Cancer Genomics and Proteomics網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Cancer Genomics and Proteomics被引頻次
書目名稱Cancer Genomics and Proteomics被引頻次學(xué)科排名
書目名稱Cancer Genomics and Proteomics年度引用
書目名稱Cancer Genomics and Proteomics年度引用學(xué)科排名
書目名稱Cancer Genomics and Proteomics讀者反饋
書目名稱Cancer Genomics and Proteomics讀者反饋學(xué)科排名
作者: 弄臟 時間: 2025-3-21 22:55
Design of Novel Bioreductive Drugs,creen is performed in this cell line and genes that activate . expression are identified. The negative selection approach described here allows RNAi screening to be used for identifying genes controlling cell survival in cancers or perhaps other human diseases with potential in therapeutic intervent作者: 注意 時間: 2025-3-22 03:44
https://doi.org/10.1007/978-3-642-51175-2rtners to specific alpha-helical motifs. In addition to markedly improved alpha-helicity, stapled peptides also display resistance to protease cleavage and enhanced cell permeability. Most importantly, they are useful for intracellular experiments that explore the functional consequences of blocking作者: ensemble 時間: 2025-3-22 07:24
Marcel Mechali,Anne-Marie de Recondothat extend beyond modeling cancer to investigations that define new cancer genes and mechanisms of cancer progression. Together, these attributes have established the zebrafish as a robust and versatile model system for investigating cancer. In this chapter we describe methods that are used to stud作者: 我說不重要 時間: 2025-3-22 12:25 作者: 使虛弱 時間: 2025-3-22 15:48 作者: 使虛弱 時間: 2025-3-22 18:23 作者: Cerebrovascular 時間: 2025-3-22 22:38
A Diphtheria Toxin Negative Selection in RNA Interference Screening,creen is performed in this cell line and genes that activate . expression are identified. The negative selection approach described here allows RNAi screening to be used for identifying genes controlling cell survival in cancers or perhaps other human diseases with potential in therapeutic intervent作者: 極端的正確性 時間: 2025-3-23 05:06
Synthesis of Stabilized Alpha-Helical Peptides,rtners to specific alpha-helical motifs. In addition to markedly improved alpha-helicity, stapled peptides also display resistance to protease cleavage and enhanced cell permeability. Most importantly, they are useful for intracellular experiments that explore the functional consequences of blocking作者: Additive 時間: 2025-3-23 07:09
Zebrafish as a Platform to Study Tumor Progression,that extend beyond modeling cancer to investigations that define new cancer genes and mechanisms of cancer progression. Together, these attributes have established the zebrafish as a robust and versatile model system for investigating cancer. In this chapter we describe methods that are used to stud作者: cultivated 時間: 2025-3-23 09:51 作者: 鞠躬 時間: 2025-3-23 14:03 作者: Chameleon 時間: 2025-3-23 18:48
Narendra WajapeyeeIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: 量被毀壞 時間: 2025-3-24 01:31
Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/221117.jpg作者: 黃油沒有 時間: 2025-3-24 05:01
https://doi.org/10.1007/978-981-33-4676-5de localization of histone modifications, histone variants, and other chromatin-associating factors. In brief, chromatin pellets are fractionated from the nuclei, and then fragmented by enzymatic digestion or sonication. Chromatin regions associated with proteins of interest are enriched by immunopr作者: Minikin 時間: 2025-3-24 10:28 作者: Engaging 時間: 2025-3-24 13:37 作者: occult 時間: 2025-3-24 18:20 作者: 整頓 時間: 2025-3-24 21:37
Design of Novel Bioreductive Drugs,r upstream activators that are essential for the survival of cancer cells often dictate cancer formation/progression. Hence, they are preferable therapeutic targets. Identifying these genes using RNAi is, however, problematic because knocking them down leads to cell death. Here we describe a diphthe作者: Daily-Value 時間: 2025-3-25 00:16 作者: Amplify 時間: 2025-3-25 04:27
https://doi.org/10.1007/978-3-642-51175-2en coordinating homologous recombination events. Here, we describe the use of TFOs such as peptide nucleic acids for targeted genome modification. We discuss this method and its applications and describe protocols for TFO design, delivery, and evaluation of activity in vitro and in vivo.作者: 地殼 時間: 2025-3-25 07:52
https://doi.org/10.1007/978-3-642-51175-2eir secondary structure when isolated from the host protein, stapled peptides incorporate an all-hydrocarbon “staple” that reinforces their natural alpha-helical structure. Thus, stapled peptides retain their functional ability to bind their native protein targets and serve multiple experimental use作者: subacute 時間: 2025-3-25 15:15 作者: Compass 時間: 2025-3-25 19:49 作者: Laconic 時間: 2025-3-25 20:23 作者: 事物的方面 時間: 2025-3-26 01:28 作者: arterioles 時間: 2025-3-26 07:23
Marcel Mechali,Anne-Marie de Recondosponse to therapy, or to serve as therapeutic targets (Chapman et al., Ann Oncol 18(5):868–873, 2007; Soussi et al., Cancer Res 60:1777–1788, 2000; Anderson and LaBaer, J Proteome Res 4:1123–1133, 2005; Levenson, Biochim Biophy Acta 1770:847–856, 2007). In cancer endogenous levels of protein express作者: 漂泊 時間: 2025-3-26 10:07
https://doi.org/10.1007/978-3-319-70211-7ransgenic mouse models, coupled with mass spectrometry proteomics, have served as valuable platform for elucidating the in vivo function of individual genes and proteins. Here we discuss the methods we have recently employed to characterize protein–protein interactions and posttranslational modifica作者: 消極詞匯 時間: 2025-3-26 13:19
https://doi.org/10.1007/978-3-319-70211-7, soluble in the cytosol or associated to cellular membranes. Importantly, their membrane-inserted form is the main responsible for their apoptotic function. Unfortunately, there are only a limited number of methods available to study the membrane activity of these proteins. Here, we present a metho作者: 下船 時間: 2025-3-26 19:23
New Approaches in Intelligent Controlification arise when proteins are of low abundance or are unstable resulting in low yields or poor in vitro activity. In this protocol we describe a method to purify active, recombinant human proteins fused to a tandem MBP tag after expression in human 293T cells.作者: 幼稚 時間: 2025-3-26 21:36
Vladimir Kanchev,Roumen Kountcheval methods that use exome sequencing reads from cancer samples to identify somatic single nucleotide variants (SNVs), copy number alterations, and short insertions and deletions (InDels). We further describe analytical methods to generate lists of driver genes with more mutational events than expect作者: Torrid 時間: 2025-3-27 04:17 作者: 驚惶 時間: 2025-3-27 07:47
Cancer Genomics and Proteomics978-1-4939-0992-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: FIR 時間: 2025-3-27 12:04 作者: 豐富 時間: 2025-3-27 16:48
New Approaches in Intelligent Controlification arise when proteins are of low abundance or are unstable resulting in low yields or poor in vitro activity. In this protocol we describe a method to purify active, recombinant human proteins fused to a tandem MBP tag after expression in human 293T cells.作者: Gratuitous 時間: 2025-3-27 18:48 作者: absolve 時間: 2025-3-27 23:11
https://doi.org/10.1007/978-1-4939-0992-6cancer cells; cancer genome; genome; progression; proteome; transcriptome; tumor cells; tumor initiation; Pr作者: 大范圍流行 時間: 2025-3-28 05:19
978-1-4939-4654-9Springer Science+Business Media New York 2014作者: DUCE 時間: 2025-3-28 06:45
Targeted Genome Modification via Triple Helix Formation,en coordinating homologous recombination events. Here, we describe the use of TFOs such as peptide nucleic acids for targeted genome modification. We discuss this method and its applications and describe protocols for TFO design, delivery, and evaluation of activity in vitro and in vivo.作者: minimal 時間: 2025-3-28 14:20
Purification of Recombinant 2XMBP Tagged Human Proteins from Human Cells,ification arise when proteins are of low abundance or are unstable resulting in low yields or poor in vitro activity. In this protocol we describe a method to purify active, recombinant human proteins fused to a tandem MBP tag after expression in human 293T cells.作者: Jacket 時間: 2025-3-28 16:11
Computational Analysis in Cancer Exome Sequencing,al methods that use exome sequencing reads from cancer samples to identify somatic single nucleotide variants (SNVs), copy number alterations, and short insertions and deletions (InDels). We further describe analytical methods to generate lists of driver genes with more mutational events than expected by chance.作者: Robust 時間: 2025-3-28 19:06
Hans Stahl,Herbert K?nig,Rolf Knippersal F. or non-clonal genetic screen. Here we describe an F. or clonal screen in the nematode . to search for genes that modify partially penetrant phenotypes. Specifically we describe a screen to search for modifiers of genes that cause defects in migration of a specific developmentally regulated cell, the distal tip cell.作者: 物種起源 時間: 2025-3-29 02:49 作者: Peculate 時間: 2025-3-29 06:24 作者: degradation 時間: 2025-3-29 10:30
Book 2014Latest editionminated us about the changes in cancer cells. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips作者: malign 時間: 2025-3-29 11:37
1064-3745 ation advice from the experts.Cancer Genomics and Proteomics: Methods and Protocols, Second Edition.?includes methods for the analyses of cancer genome and proteome that have illuminated us about the changes in cancer cells. Written in the highly successful .Methods in Molecular Biology .series form作者: optic-nerve 時間: 2025-3-29 17:32
Hein Venter,Mariki Eloff,Rossouw Solmse a detailed protocol for using the Hannon–Elledge miR30-based library to conduct dropout screens in cancer cell lines. This protocol is readily adaptable to other pooled shRNA libraries and should facilitate the functional annotation of the human genome.作者: 使苦惱 時間: 2025-3-29 23:06
Discovery of Improved Platinum Analogues,thway leads to elevated posttranslational addition of O-linked-β-.-acetylglucosamine (O-GlcNAc) on a diverse population of nuclear and cytosolic proteins, many of which regulate signaling pathways. This unit outlines techniques used to detect metabolic alterations in cancer cells, regulation by signaling pathways, and cellular O-GlcNAcylation.作者: 繞著哥哥問 時間: 2025-3-30 02:24
https://doi.org/10.1007/978-3-319-70211-7 genes and proteins. Here we discuss the methods we have recently employed to characterize protein–protein interactions and posttranslational modifications in tagged knock-in mouse models. These methods can be broadly applied to other systems for various applications in both basic and translational science.作者: 責(zé)任 時間: 2025-3-30 04:53 作者: 鐵塔等 時間: 2025-3-30 11:48 作者: accordance 時間: 2025-3-30 13:37
,Interrogation of In Vivo Protein–Protein Interactions Using Transgenic Mouse Models and Stable Isot genes and proteins. Here we discuss the methods we have recently employed to characterize protein–protein interactions and posttranslational modifications in tagged knock-in mouse models. These methods can be broadly applied to other systems for various applications in both basic and translational science.作者: OFF 時間: 2025-3-30 17:55 作者: 領(lǐng)帶 時間: 2025-3-30 23:55
https://doi.org/10.1007/978-981-97-0109-4or the acquisition of comprehensive information of the methylation landscape in diseases like cancer. Data generated by this approach is typically reproducible and often covers between 65 and 75 % of the whole genome.作者: 蕨類 時間: 2025-3-31 01:54
https://doi.org/10.1007/978-981-97-0109-4m total RNA samples and on-site biochemical reactions, coupled with bead-based detection in 96-well format for hundreds of miRNAs per sample. With low-cost, high-throughput, high detection specificity, and flexibility to profile both small and large numbers of samples, this protocol can be adapted in a wide range of laboratory settings.作者: 使激動 時間: 2025-3-31 07:14 作者: Halfhearted 時間: 2025-3-31 10:12 作者: 勤勞 時間: 2025-3-31 15:45 作者: VEN 時間: 2025-3-31 18:06 作者: semble 時間: 2025-4-1 00:07 作者: BULLY 時間: 2025-4-1 02:28
A High-Throughput MicroRNA Expression Profiling System,m total RNA samples and on-site biochemical reactions, coupled with bead-based detection in 96-well format for hundreds of miRNAs per sample. With low-cost, high-throughput, high detection specificity, and flexibility to profile both small and large numbers of samples, this protocol can be adapted in a wide range of laboratory settings.作者: 剛毅 時間: 2025-4-1 06:27 作者: CHAFE 時間: 2025-4-1 11:06
Using LacO Arrays to Monitor DNA Double-Strand Break Dynamics in Live , Cells,cO arrays with targeting efficiencies as high as 70 %. Combining this facile LacO/LacI-GFP system with a site-specific, inducible DSB provides a means to monitor DSB dynamics at engineered sites within the genome.作者: Vulvodynia 時間: 2025-4-1 14:47
New Biophysical Methods to Study the Membrane Activity of Bcl-2 Proteins,lamellar vesicles and involves directly visualization of the process with a confocal microscope. This approach allows for the characterization of the membrane activity of the Bcl-2 proteins (or of any other pore-forming molecule) with unprecedented detail.作者: grenade 時間: 2025-4-1 19:58 作者: apropos 時間: 2025-4-2 00:58
Reduced Representation Bisulfite Sequencing to Identify Global Alteration of DNA Methylation,ucleotide level. DNA methylation status is determined by utilizing DNA methylation-specific restriction enzymes to selectively amplify for genomic regions that are rich in methylated DNA. Although the method is genome-wide, DNA methyl sequencing does not require the sequencing of the whole genome, h作者: 潛移默化 時間: 2025-4-2 04:55 作者: 檢查 時間: 2025-4-2 08:09
