標(biāo)題: Titlebook: Cancer Cell Signaling; Methods and Protocol David M. Terrian Book 2003 Humana Press 2003 [打印本頁] 作者: CLIP 時(shí)間: 2025-3-21 16:06
書目名稱Cancer Cell Signaling影響因子(影響力)
作者: 擔(dān)憂 時(shí)間: 2025-3-21 23:26 作者: 鞏固 時(shí)間: 2025-3-22 01:37 作者: 策略 時(shí)間: 2025-3-22 05:28 作者: Expertise 時(shí)間: 2025-3-22 10:20 作者: Dysarthria 時(shí)間: 2025-3-22 16:42 作者: Dysarthria 時(shí)間: 2025-3-22 19:07
TUNEL and Immunofluorescence Double-Labeling Assay for Apoptotic Cells with Specific Antigen(s)ssay, and antigens of interest, using immunofluorescence, is described. The assay has been used successfully on fixed, cultured cells and formalin-fixed paraffin-embedded tissue sections. This . detection system can be used to correlate apoptosis with specific antigen expression to identify factors 作者: 無情 時(shí)間: 2025-3-22 22:49 作者: 陶瓷 時(shí)間: 2025-3-23 04:45
Protein Tyrosine Kinase and Phosphatase Expression Profiling in Human Cancerse of the large number of tyrosine kinase/phosphatase genes in such gene family, it is essential to use an efficient and simple approach to obtain comprehensive protein tyrosine kinase and protein tyrosine phosphatase expression profiles. Knowledge of such an overall expression pattern of tyrosine ki作者: 煩人 時(shí)間: 2025-3-23 06:34
Association of Nonreceptor Tyrosine Kinase ,-Yes with Tight Junction Protein Occludin by Coimmunoprean immunoprecipitation procedure: 1) lysis of the cells to make the antigen of interest available; 2) formation of the antibody-antigen complex by adding specific antibody; 3) separation and detection of the immune complex by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and i作者: delusion 時(shí)間: 2025-3-23 13:09
Manipulating Expression of Endogenous Oncogenic Proteins Using an Antisense Oligonucleotide Approachoteins. Using this method, we have acquired additional evidence that protein kinase C-epsilon functions as an oncogenic protein in the progression of recurrent human prostate cancer. This chapter describes the use of antisense ODN to directly target cellular protein kinase C-epsilon as a potential c作者: ANA 時(shí)間: 2025-3-23 17:31
Measurements of Phospholipases A2, C, and D (PLA2, PLC, and PLD)to mitosis. Phospholipases A., C, and D (PLA., PLC, and PLD) are involved in regulatory events of intracellular mitogen signaling pathways. We describe here three methods for comprehensively assaying those phospholipases: 1) in vitro microassays, in which a radiolabeled substrate is exogenously adde作者: hieroglyphic 時(shí)間: 2025-3-23 20:04 作者: 舊石器 時(shí)間: 2025-3-24 01:43 作者: 憤慨點(diǎn)吧 時(shí)間: 2025-3-24 04:44 作者: 平庸的人或物 時(shí)間: 2025-3-24 07:07 作者: 職業(yè)拳擊手 時(shí)間: 2025-3-24 12:43 作者: 影響帶來 時(shí)間: 2025-3-24 16:11
Introduction to the Study of Stress,it allows the measurement of, in parallel, the activities of three phospholipases utilizing aliquots derived from . biological sample. The approach for immunoprecipitation described in this chapter can be extrapolated to the study of a large array of enzyme isoforms. Finally, the intact-cell assays 作者: Spinal-Fusion 時(shí)間: 2025-3-24 20:36
https://doi.org/10.1007/978-3-031-00864-1ed by adding to EMSA an antibody specific for that protein, which will result in supershift complexes on the gel. The presence of the proteins in question can be further verified by standard Western analysis of the nuclear proteins.作者: Odyssey 時(shí)間: 2025-3-25 02:00
Association of Nonreceptor Tyrosine Kinase ,-Yes with Tight Junction Protein Occludin by Coimmunopreterest is tyrosine phosphorylated, and this tyrosine phosphorylation is under investigation, each step has to be handled with extra care to ensure success of the procedure. The buffer used to lyse the cells depends on the nature of the antigen, with more stringent conditions required for integral me作者: 贊美者 時(shí)間: 2025-3-25 03:25
Measurements of Phospholipases A2, C, and D (PLA2, PLC, and PLD)it allows the measurement of, in parallel, the activities of three phospholipases utilizing aliquots derived from . biological sample. The approach for immunoprecipitation described in this chapter can be extrapolated to the study of a large array of enzyme isoforms. Finally, the intact-cell assays 作者: 輕彈 時(shí)間: 2025-3-25 09:01
Detection of the Content and Activity of the Transcription Factor AP-1 in a Multistage Skin Carcinoged by adding to EMSA an antibody specific for that protein, which will result in supershift complexes on the gel. The presence of the proteins in question can be further verified by standard Western analysis of the nuclear proteins.作者: 啟發(fā) 時(shí)間: 2025-3-25 12:11
1064-3745 olution of this field is provided by an analysis of the Human Genome Project data, which reveal a previously unsuspected diversity in the multigene families encoding for most signaling pathway 978-1-61737-341-1978-1-59259-356-9Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 跳脫衣舞的人 時(shí)間: 2025-3-25 17:59 作者: 樹木中 時(shí)間: 2025-3-25 21:04
Isolation of Novel Substrates Using a Tyrosine Kinase Overlay/, Assay作者: 性學(xué)院 時(shí)間: 2025-3-26 02:09
1064-3745 ly maintain a critical balance between cellular proliferation, differentiation, senescence, and death. One current research challenge is to identify those aberrations in signal transd- tion that directly contribute to a loss of this division-limited equilibrium and the progression to malignant trans作者: 法律的瑕疵 時(shí)間: 2025-3-26 08:14 作者: 殘酷的地方 時(shí)間: 2025-3-26 09:23
Manfred Laucht,Daniel Brandeis,Katrin Zohseled paraffin-embedded tissue sections. This . detection system can be used to correlate apoptosis with specific antigen expression to identify factors involved in the induction and execution of the apoptotic pathway of cell death in a wide variety of experimental systems.作者: Flinch 時(shí)間: 2025-3-26 12:52 作者: judicial 時(shí)間: 2025-3-26 19:27 作者: vanquish 時(shí)間: 2025-3-26 21:59 作者: troponins 時(shí)間: 2025-3-27 02:31
Generation of Axons and Dendrites of, protein-protein interactions in cells that do not typically occur, or are weaker, under normal redox conditions. In this chapter, I describe a method to measure the strength of protein-protein interactions that may be enhanced during oxidative stress using the yeast two-hybrid system.作者: MENT 時(shí)間: 2025-3-27 07:32 作者: LAP 時(shí)間: 2025-3-27 09:38 作者: adulterant 時(shí)間: 2025-3-27 16:09 作者: 北極人 時(shí)間: 2025-3-27 18:52
Regressive Phenomena: Refining Connectionssion. One is based on BrdU incorporation (i.e., DNA replication) and the other on flow cytometry. Together, this triad of techniques is sufficient to determine the action of tumor suppressors and other antiproliferative agents.作者: 減弱不好 時(shí)間: 2025-3-28 01:09 作者: Paradox 時(shí)間: 2025-3-28 04:54
Neuroscience in the 21st Centuryce in human neuroblastoma cells to doxorubicin, a topoisomerase-2 inhibitor, and to MDL-28842, an inhibitor of S-adenosylhomocysteine hydrolase, and reductions in the activation and nuclear translocation of MAPk.作者: PET-scan 時(shí)間: 2025-3-28 09:02
Eric D. Finegood,Gregory E. Millere-modified antisense ODNs were transferred into prostate cancer cells with high efficiency, effectively inhibiting the expression of endogenous protein kinase C-epsilon and the androgen-independent (AI) proliferation of several independent human prostate cancer cell lines.作者: motivate 時(shí)間: 2025-3-28 13:18
Introduction to the Study of Stress,ocus on basic tissue culture techniques which are used to elucidate signal transduction pathways. Furthermore, this chapter will provide a general background for understanding how gene transfer techniques can be used to elucidate signal transduction pathways as well as various pitfalls commonly encountered with their usage.作者: 作繭自縛 時(shí)間: 2025-3-28 15:56
Functional Analysis of the Antimitogenic Activity of Tumor Suppressorssion. One is based on BrdU incorporation (i.e., DNA replication) and the other on flow cytometry. Together, this triad of techniques is sufficient to determine the action of tumor suppressors and other antiproliferative agents.作者: VAN 時(shí)間: 2025-3-28 21:19
Signal Transduction Study Using Gene-Targeted Embryonic Stem Cellsetermine the role of the genes in the mediation of various cellular activities such as proliferation, differentiation, apoptosis, survival, transformation, and so on. Furthermore, with the recent advance of in vitro differentiation techniques, it is now feasible to rapidly determine the role of specific molecules in particular tissues.作者: 遣返回國 時(shí)間: 2025-3-28 22:55 作者: institute 時(shí)間: 2025-3-29 04:21
Manipulating Expression of Endogenous Oncogenic Proteins Using an Antisense Oligonucleotide Approache-modified antisense ODNs were transferred into prostate cancer cells with high efficiency, effectively inhibiting the expression of endogenous protein kinase C-epsilon and the androgen-independent (AI) proliferation of several independent human prostate cancer cell lines.作者: 沉默 時(shí)間: 2025-3-29 10:57 作者: flaunt 時(shí)間: 2025-3-29 14:56
Signal Transduction-Dependent Channelsnes by determining the retention of prostate-specific epithelial expression markers, including cytokeratins, the androgen receptor, estrogen receptor β and the dorsolateral prostatic secretory protein (mDLP) are given.作者: BRIEF 時(shí)間: 2025-3-29 18:44 作者: NAG 時(shí)間: 2025-3-29 23:41
Jonathan Buening,Rhonda Douglas Brownrotein tyrosine kinase/phosphatase molecules from cancer cells by reverse-transcription polymerase-chain-reaction. An improved profiling approach (RAGE) was also described by utilizing restriction enzyme digestion and electrophoresis for quick and efficient kinase/phosphatase profiling.作者: 針葉類的樹 時(shí)間: 2025-3-30 00:03
Rescue and Isolation of ,-deficient Prostate Epithelium by Tissue Recombinationnes by determining the retention of prostate-specific epithelial expression markers, including cytokeratins, the androgen receptor, estrogen receptor β and the dorsolateral prostatic secretory protein (mDLP) are given.作者: Mercurial 時(shí)間: 2025-3-30 05:35 作者: 收養(yǎng) 時(shí)間: 2025-3-30 11:04
Protein Tyrosine Kinase and Phosphatase Expression Profiling in Human Cancersrotein tyrosine kinase/phosphatase molecules from cancer cells by reverse-transcription polymerase-chain-reaction. An improved profiling approach (RAGE) was also described by utilizing restriction enzyme digestion and electrophoresis for quick and efficient kinase/phosphatase profiling.作者: 清楚說話 時(shí)間: 2025-3-30 13:05
Regressive Phenomena: Refining Connectionsctivity and halt cell-cycle progression. In this chapter, we present three methods that have been utilized to monitor the antimitogenic action exerted by tumor suppressors. Tumor suppressor function can be demonstrated by colony formation assays and acquisition of the flat-cell phenotype. Because of作者: 沒有準(zhǔn)備 時(shí)間: 2025-3-30 18:18 作者: confederacy 時(shí)間: 2025-3-31 00:29 作者: 口訣法 時(shí)間: 2025-3-31 01:17 作者: Metamorphosis 時(shí)間: 2025-3-31 08:49 作者: foodstuff 時(shí)間: 2025-3-31 10:45
Neuroscience in the 21st Century resistance. In neuroblastomas there is evidence that acquired drug resistance may be associated with altered response to growth factor signals. The ubiquitous mitogen-activated protein kinase (MAPk) cascade, which transmits growth factor signals from the cell membrane to the nucleus, provides a pri作者: 慷慨不好 時(shí)間: 2025-3-31 14:55
