標(biāo)題: Titlebook: Cancer Cell Biology; Methods and Protocol Sherri L. Christian Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive li [打印本頁] 作者: 筆記 時(shí)間: 2025-3-21 17:31
書目名稱Cancer Cell Biology影響因子(影響力)
書目名稱Cancer Cell Biology影響因子(影響力)學(xué)科排名
書目名稱Cancer Cell Biology網(wǎng)絡(luò)公開度
書目名稱Cancer Cell Biology網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Cancer Cell Biology被引頻次
書目名稱Cancer Cell Biology被引頻次學(xué)科排名
書目名稱Cancer Cell Biology年度引用
書目名稱Cancer Cell Biology年度引用學(xué)科排名
書目名稱Cancer Cell Biology讀者反饋
書目名稱Cancer Cell Biology讀者反饋學(xué)科排名
作者: Nerve-Block 時(shí)間: 2025-3-22 00:06
https://doi.org/10.1007/978-1-0716-2376-3Biobanking; chemotaxis assay; Flow-cytometry; CRISPR/Cas; Immunofluorescence作者: panorama 時(shí)間: 2025-3-22 02:38
978-1-0716-2378-7The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines作者: glisten 時(shí)間: 2025-3-22 05:52
Sherri L. ChristianIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: Ancestor 時(shí)間: 2025-3-22 09:14
Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/221058.jpg作者: 走調(diào) 時(shí)間: 2025-3-22 16:07
Cancer Cell Biology978-1-0716-2376-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 走調(diào) 時(shí)間: 2025-3-22 18:59
Nicholas L. Zalewski,Sean J. Pittockancer. Here we describe a method using tandem mass tag (TMT) technology to multiplex up to 18 samples in a single analysis, paving the way for the analysis of large cohorts of tumors, cell lines, and perturbations thereof. The procedure we describe will result in samples ready for in-depth LC-MS/MS analysis in 3–4?days.作者: Nausea 時(shí)間: 2025-3-22 21:30
Robert L. Sainburg,Pratik K. Muthas and maintaining records on usage and confirmation of identity. Cross-contamination of cancer cell lines has been well documented by the ATCC and must be avoided at all costs. In addition, contamination by . sp. is a well-known issue, which, if left unchecked, can affect all data collected as this 作者: needle 時(shí)間: 2025-3-23 03:19 作者: cultivated 時(shí)間: 2025-3-23 07:57
Volker Dietz,Tobias Nef,William Zev Rymerired to lift the cells from the cell culture vessel for harvesting or subculturing. Many cancer cell lines are adherent, rendering adherent cell culture a critical experimental method in the fields of cell biology, biochemistry, and cancer research. In this chapter, we outline the protocols for cult作者: 旅行路線 時(shí)間: 2025-3-23 10:07
Passive Devices for Upper Limb Trainingd immunocompromised mouse models are used depending on the scope of the study. Immunocompetent models allow the study of the impact of the immune system in murine models of mammary cancer, while immunodeficient mice serve as ideal host organisms to understand the behavior of human breast cancers wit作者: 獨(dú)白 時(shí)間: 2025-3-23 16:31 作者: Debate 時(shí)間: 2025-3-23 21:31
https://doi.org/10.1007/978-1-4471-2277-7proximity. Here we describe co-culture of Simpson–Golabi–Behmel syndrome (SGBS) adipocyte cells with the MCF-7 breast cancer cell line using the Corning. Transwell. 12-mm, 0.4-μm pore polyester membrane insert cell culture system. The SGBS adipocyte cell line, which was developed from cells taken fr作者: 膠狀 時(shí)間: 2025-3-24 00:59 作者: 偽善 時(shí)間: 2025-3-24 02:35 作者: 詳細(xì)目錄 時(shí)間: 2025-3-24 10:25 作者: apiary 時(shí)間: 2025-3-24 13:56
https://doi.org/10.1007/978-3-319-58738-7ith advances in analytical methods, ELISA assay has been constantly optimized to improve its sensitivity, and different types of ELISA are now available to detect various biomarkers. This chapter provides an overall summary of the basic principle of ELISA, discusses different components of ELISA ass作者: TIGER 時(shí)間: 2025-3-24 17:08
Alessia Pellerino,Riccardo Soffietti number of positive cells and their expression levels. Loss or downregulation of HLA-I molecules on cancer cells, a well-documented immune escape mechanism, may occur via activation and integration of numerous signalling pathways that are operative in cancer. Whereas IFN-γ, produced during an adapti作者: 舊石器 時(shí)間: 2025-3-24 20:53 作者: 抓住他投降 時(shí)間: 2025-3-25 01:01
9 Functioneel oefenen en motorisch leren,preserved tissues available, there are possibilities to assess large cohorts using archived tissue which may have archived associated clinical outcomes. Although best practice guidelines for the assessment of tissues have been published, a standardized method for immunofluorescence of FFPE tumor tis作者: 字謎游戲 時(shí)間: 2025-3-25 05:53
Nicole Yang,Jonathan Scott Coblyno system, we prepared whole cell lysates from HER2-overexpressing breast cancer cell lines, treated or not with the tyrosine kinase inhibitor, lapatinib, in the presence and absence of IFN-γ. Here we describe the protocol whereby proteins in the lysates were separated by SDS-PAGE, electrophoreticall作者: 監(jiān)禁 時(shí)間: 2025-3-25 10:35 作者: modish 時(shí)間: 2025-3-25 15:30 作者: BRINK 時(shí)間: 2025-3-25 19:47
The Hierarchical Nature of Brain Dynamics,uring cellular metabolism is therefore central to understanding the cellular physiology of cancer cells. The Seahorse XFe Analyzer series allows real-time measurement of cellular metabolism. In the basic assay, two parameters, the oxygen consumption rate (OCR) and the extracellular acidification rat作者: 有特色 時(shí)間: 2025-3-25 21:32 作者: 蔓藤圖飾 時(shí)間: 2025-3-26 01:04
The Hierarchical Nature of Brain Dynamics,olution. The Fluidigm C1 is an automated system for preparing complementary deoxyribonucleic acid (cDNA) samples that have been synthesized and preamplified from single cells for next-generation sequencing (NGS) analysis. Herein, we detail a workflow and protocol for scRNA-seq analysis of 3′-end enr作者: –DOX 時(shí)間: 2025-3-26 05:24 作者: LEER 時(shí)間: 2025-3-26 10:12
Culturing Suspension Cancer Cell Lines,c cells that exist in the circulation of multicellular animals such as mouse and humans. Generally, cell lines derived from the blood such as lymphocytes, megakaryocyte, and neutrophils grow in suspension. These cell lines can be used for experimental studies to understand the biology/biochemistry o作者: oracle 時(shí)間: 2025-3-26 14:38 作者: Tinea-Capitis 時(shí)間: 2025-3-26 19:22
Breast Cancer Xenograft Murine Models,d immunocompromised mouse models are used depending on the scope of the study. Immunocompetent models allow the study of the impact of the immune system in murine models of mammary cancer, while immunodeficient mice serve as ideal host organisms to understand the behavior of human breast cancers wit作者: NEXUS 時(shí)間: 2025-3-26 22:26
Processing and Cryopreservation of Blood, Cancer Tissues, and Cancer Cells for Viable Biobanking,ions of these materials, however, do not enable the establishment of short-term cultures, cell lines, or patient-derived xenograft models for functional studies. Also, intact dissociated cells that are required for some single-cell analyses cannot be obtained from these material types. Adding viable作者: Mendacious 時(shí)間: 2025-3-27 04:23
,Adipocyte–Breast Cancer Cell Co-Culture in Transwells,proximity. Here we describe co-culture of Simpson–Golabi–Behmel syndrome (SGBS) adipocyte cells with the MCF-7 breast cancer cell line using the Corning. Transwell. 12-mm, 0.4-μm pore polyester membrane insert cell culture system. The SGBS adipocyte cell line, which was developed from cells taken fr作者: 白楊 時(shí)間: 2025-3-27 06:40 作者: nocturia 時(shí)間: 2025-3-27 12:28 作者: jumble 時(shí)間: 2025-3-27 15:29 作者: 修剪過的樹籬 時(shí)間: 2025-3-27 18:43
Enzyme-Linked Immunosorbent Assay (ELISA),ith advances in analytical methods, ELISA assay has been constantly optimized to improve its sensitivity, and different types of ELISA are now available to detect various biomarkers. This chapter provides an overall summary of the basic principle of ELISA, discusses different components of ELISA ass作者: intricacy 時(shí)間: 2025-3-28 01:52
,Flow Cytometry Analysis to Detect Lapatinib-Induced Modulation of Constitutive and IFN-γ-Induced HL number of positive cells and their expression levels. Loss or downregulation of HLA-I molecules on cancer cells, a well-documented immune escape mechanism, may occur via activation and integration of numerous signalling pathways that are operative in cancer. Whereas IFN-γ, produced during an adapti作者: Influx 時(shí)間: 2025-3-28 04:59
An End-to-End Workflow for Interrogating Tumor-Infiltrating Myeloid Cells Using Mass Cytometry,ts can impact tumor progression differently, with conventional type 1 DCs important in protective anti-tumor immune responses, while immunosuppressive tumor-associated macrophages and myeloid-derived suppressive cells (MDSCs) play tumor-promoting roles. Deep phenotyping of myeloid cells using single作者: inclusive 時(shí)間: 2025-3-28 09:07 作者: 身體萌芽 時(shí)間: 2025-3-28 10:24 作者: 著名 時(shí)間: 2025-3-28 16:51
Evaluating Cell Membrane Localization and Intracellular Transport of Proteins by Biotinylation,Abnormal protein localization can affect key cell functions, including transduction of extracellular signals and organization of the cytoskeleton, significantly affecting oncogenicity and therapeutic responses. In this chapter, we describe a surface protein biotinylation method that allows the study作者: FLACK 時(shí)間: 2025-3-28 22:15 作者: Spina-Bifida 時(shí)間: 2025-3-29 02:48
The Use of Seahorse XF Assays to Interrogate Real-Time Energy Metabolism in Cancer Cell Lines,uring cellular metabolism is therefore central to understanding the cellular physiology of cancer cells. The Seahorse XFe Analyzer series allows real-time measurement of cellular metabolism. In the basic assay, two parameters, the oxygen consumption rate (OCR) and the extracellular acidification rat作者: 咯咯笑 時(shí)間: 2025-3-29 06:50 作者: LEVER 時(shí)間: 2025-3-29 08:44 作者: Talkative 時(shí)間: 2025-3-29 14:38
1064-3745 ology: Methods and Protocols.?aims?to?provide a?..comprehensive set of tools for the analysis of cancer cell biology in the lab..978-1-0716-2378-7978-1-0716-2376-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: perpetual 時(shí)間: 2025-3-29 15:53 作者: crescendo 時(shí)間: 2025-3-29 23:02
,Flow Cytometry Analysis to Detect Lapatinib-Induced Modulation of Constitutive and IFN-γ-Induced HLy data clearly show lapatinib-mediated downregulation of both constitutive and IFN-γ-induced HLA class I expression. These results, for which we do not yet have an explanation, may have important implications for our understanding of lapatinib resistance in metastatic HER2+ cancer.作者: 懶惰人民 時(shí)間: 2025-3-30 00:19
Manual Immunofluorescence of Formalin-Fixed Paraffin-Embedded Human Tumor Tissues,y and highly accessible method for immunofluorescence of FFPE-embedded tissues, hoping to enable more laboratories to take on this highly informative technique and empower them to begin IF analysis in their own tissues of interest.作者: 有偏見 時(shí)間: 2025-3-30 07:26
The Use of Seahorse XF Assays to Interrogate Real-Time Energy Metabolism in Cancer Cell Lines,e Seahorse Real-Time ATP Rate Assay for adherent and suspension cancer cell lines. Depending on individual experimental results, more refined subsequent assays can then be performed to specifically determine, for example, the ability to utilize different substrates by the cell lines in the presence 作者: 投票 時(shí)間: 2025-3-30 10:22
https://doi.org/10.1007/978-1-4471-2277-7membrane and in the well beneath. This co-culture procedure described here can be applied to analyze the effects of cancer cells on the process of adipogenesis and the changes in cancer cells due to adipocyte-secreted factors.作者: In-Situ 時(shí)間: 2025-3-30 15:50 作者: 持久 時(shí)間: 2025-3-30 18:40 作者: 暫時(shí)中止 時(shí)間: 2025-3-31 00:33
The Hierarchical Nature of Brain Dynamics,e Seahorse Real-Time ATP Rate Assay for adherent and suspension cancer cell lines. Depending on individual experimental results, more refined subsequent assays can then be performed to specifically determine, for example, the ability to utilize different substrates by the cell lines in the presence 作者: 帽子 時(shí)間: 2025-3-31 02:03 作者: declamation 時(shí)間: 2025-3-31 06:21 作者: 越自我 時(shí)間: 2025-3-31 11:16 作者: 獸皮 時(shí)間: 2025-3-31 17:21
Symptomatik von Demenz in der Praxis,ultiple cell types under the influence of the ECM. Here we describe a 3D co-culture technique for studying breast cancer—adipocyte interactions. This technique could easily be modified to analyze interactions between other cancer cell types and different fibroblast-like cells.作者: enflame 時(shí)間: 2025-3-31 19:53
Culturing Suspension Cancer Cell Lines,reserving cancer cell lines. Importantly, this chapter demonstrates the best practices required to work with suspension cell lines, to minimize the risk of contaminations from adventitious microorganisms or from other cell lines.作者: 碳水化合物 時(shí)間: 2025-4-1 00:11
Culture of Adherent Cancer Cell Lines,ring adherent cells, freezing cells, and counting cells. Most notably, we outline the best techniques and practices for optimal growth of healthy adherent cells while diminishing the risk of contamination.
