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標題: Titlebook: Calcium-Binding Protein Protocols; Volume 2: Methods an Hans J. Vogel Book 2002 Humana Press 2002 [打印本頁]

作者: 退縮    時間: 2025-3-21 20:00
書目名稱Calcium-Binding Protein Protocols影響因子(影響力)




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書目名稱Calcium-Binding Protein Protocols網(wǎng)絡(luò)公開度學(xué)科排名




書目名稱Calcium-Binding Protein Protocols被引頻次




書目名稱Calcium-Binding Protein Protocols被引頻次學(xué)科排名




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書目名稱Calcium-Binding Protein Protocols年度引用學(xué)科排名




書目名稱Calcium-Binding Protein Protocols讀者反饋




書目名稱Calcium-Binding Protein Protocols讀者反饋學(xué)科排名





作者: abject    時間: 2025-3-21 22:06

作者: 輕觸    時間: 2025-3-22 01:20

作者: 混合物    時間: 2025-3-22 07:48

作者: 吞噬    時間: 2025-3-22 09:34

作者: ADAGE    時間: 2025-3-22 15:09

作者: ADAGE    時間: 2025-3-22 19:24
Fluorescence Methods for Measuring Calcium Affinity and Calcium Exchange with Proteinsnd smooth muscle contraction, neurotransmission, cell proliferation, and division. Nature has designed hundreds of Ca.-binding proteins that sense the “Ca. signal” and transduce it into cellular action. Ca.-binding proteins are “tuned” to respond to these Ca.transients by virtue of their specific Ca
作者: Ablation    時間: 2025-3-22 22:23

作者: gastritis    時間: 2025-3-23 04:40
Isothermal Titration Calorimetrynteracting systems. The method of choice for the direct measurements of energetics of protein-ligand interactions is the isothermal titration calorimetry (ITC) technique. The use of ITC to measure the binding of a macromolecule (in general, a protein) to its ligand (ion, peptide, another protein, DN
作者: 同義聯(lián)想法    時間: 2025-3-23 08:38
Multiangle Laser Light Scattering and Sedimentation Equilibrium proteins and their interactions in solution under physiological conditions. Both techniques have undergone a resurgence as a result of the advent of recombinant technologies which has enabled the generation of reasonable quantities of biologically significant proteins that exist in vivo in small am
作者: Firefly    時間: 2025-3-23 12:28
Small-Angle Solution Scattering Reveals Information on Conformational Dynamics in Calcium-Binding Prce of this scattering is related to the structure of the protein. For a solution of randomly oriented proteins, the scattering is concentrated in the vicinity of the direct beam, or zero-angle. Solution scattering is therefore often referred to as small-angle or low-angle scattering. Structural info
作者: 笨拙處理    時間: 2025-3-23 15:42
Investigation of Calcium-Binding Proteins Using Electrospray Ionization Mass Spectrometrys that are used to characterize macromolecules. It is particularly suitable for studying proteins in their native state as the solvent conditions for transferring and ionizing protein molecules from solution to the gas phase can be similar to those used for characterizing proteins in solution. Follo
作者: accessory    時間: 2025-3-23 20:00
Proteolytic Fragments of Calcium-Binding Proteinsifs. These motifs predominantly exist in pairs in these proteins, which is important for high-affinity, cooperative binding of Ca.ions. Separation of individual helix-loop-helix domains through proteolysis provides an ideal starting point to asses the importance of having these domains in pairs. In
作者: 飛行員    時間: 2025-3-24 00:53

作者: Lineage    時間: 2025-3-24 02:29
Cadmium-113 and Lead-207 NMR Spectroscopic Studies of Calcium-Binding Proteinsrotein. For calcium-binding proteins, the NMR active isotope .Ca is a quadrupolar (I = 7/2) nucleus that produces broad peaks when bound to a protein (.–.) Although much useful information can be gleaned from studies of this nucleus (.,.), it is impossible to resolve the signals for individual calci
作者: AVOW    時間: 2025-3-24 06:58

作者: N防腐劑    時間: 2025-3-24 11:06
Book 2002 it modifies their biological activity or their stability. Because of its distinct che- cal properties calcium is uniquely suited to act as an on–off switch or as a light dimmer of biological activities. The two books entitled Calcium-Binding Protein Protocols (Volumes I and II) focus on modern expe
作者: Frenetic    時間: 2025-3-24 16:22

作者: 不確定    時間: 2025-3-24 20:25
Investigation of Calcium-Binding Proteins Using Electrospray Ionization Mass Spectrometrywing a study of myoglobin at the beginning of decade (.), there have been a number of reported ESI-MS studies of specific noncovalent protein-ligand complexes. A recent review by Loo provides a thorough list of ESI-MS studies of proteins interacting with metals, small molecules, peptides and proteins, and nucleic acids (.).
作者: 西瓜    時間: 2025-3-25 02:19

作者: 玉米棒子    時間: 2025-3-25 04:22

作者: miscreant    時間: 2025-3-25 10:57

作者: achlorhydria    時間: 2025-3-25 11:48
https://doi.org/10.1007/978-3-662-32776-0roteins in this class are very similar in how they coordinate the bound metal ion (apparently all octahedral). Below, we review some of these results with proteins such as calmodulin, parvalbumin, troponin C, which are contrasted with α-lactalbumin, a protein characterized by a distorted trigonal bipyramidal coordination sphere.
作者: BRUNT    時間: 2025-3-25 16:02
Beschreibung neuerer Kraftwerke,ing site from the observed shifts. .Cd, and to a lesser extent, .Pb, are two such I = 1/2 metal nuclei that have been successfully used to characterize the Ca.-binding properties of a variety of metalloproteins (.–.)
作者: Calculus    時間: 2025-3-25 23:00
https://doi.org/10.1007/978-3-662-25268-0ng with calciumbinding proteins consider the .Ca isotope as an exotic one and only a limited number of groups have been involved in .Ca NMR studies of biological systems (.-.). Only the Swedish group in Lund and the Japanese group in Sendai have been using .Ca NMR more consistently.
作者: sparse    時間: 2025-3-26 03:37
Proteolytic Fragments of Calcium-Binding Proteinspprox 35 residues) of the peptides needed. Whatever the route they obtain, isolated EF-hands are interesting models of Ca.-binding proteins. Often, these isolated motifs, such as thrombic fragements of calmodulin (CaM) (.) and synthetic troponin-C peptides (.,.,.,.,.) dimerize in vitro to form native-like structures.
作者: aptitude    時間: 2025-3-26 05:56

作者: 委托    時間: 2025-3-26 10:26
Cadmium-113 and Lead-207 NMR Spectroscopic Studies of Calcium-Binding Proteinsing site from the observed shifts. .Cd, and to a lesser extent, .Pb, are two such I = 1/2 metal nuclei that have been successfully used to characterize the Ca.-binding properties of a variety of metalloproteins (.–.)
作者: gait-cycle    時間: 2025-3-26 15:33

作者: 誘拐    時間: 2025-3-26 17:27
https://doi.org/10.1007/978-3-642-74770-0n family containing EF-hand domains (.–.). Because of its pivotal role in many Ca.-dependent cellular events, the understanding of the mechanism of action of this protein at the molecular level has been the aim of several research groups. For such a study, four main points have to be tackled:
作者: 刺耳的聲音    時間: 2025-3-26 22:42
Entwicklungsstadien der LWS im R?ntgenbildhromophore to the total absorbance of the protein will depend, to some extent at least, upon its environment. The experimentally measured parameter, the absorbance A is related to the molar extinction coefficient, ε. (.-.cm-.), the path length l (cm), and the protein concentration C (.) by the Beer-Lambert law A = .
作者: Constitution    時間: 2025-3-27 01:21

作者: Abnormal    時間: 2025-3-27 08:36
Deconvolution of Calcium-Binding Curvesn family containing EF-hand domains (.–.). Because of its pivotal role in many Ca.-dependent cellular events, the understanding of the mechanism of action of this protein at the molecular level has been the aim of several research groups. For such a study, four main points have to be tackled:
作者: irreducible    時間: 2025-3-27 11:26
Absorption and Circular Dichroism Spectroscopyhromophore to the total absorbance of the protein will depend, to some extent at least, upon its environment. The experimentally measured parameter, the absorbance A is related to the molar extinction coefficient, ε. (.-.cm-.), the path length l (cm), and the protein concentration C (.) by the Beer-Lambert law A = .
作者: 建筑師    時間: 2025-3-27 17:33

作者: Trabeculoplasty    時間: 2025-3-27 19:36

作者: 艱苦地移動    時間: 2025-3-28 00:14

作者: 牙齒    時間: 2025-3-28 05:02

作者: 刺耳的聲音    時間: 2025-3-28 06:46
M. L. Ternes,B. F. Pontz,C. R. Paterson ligand molecules by macromolecules. In this method, a semipermeable cellulose bag containing a solution of macromolecules is immersed in the buffer solution containing ligand molecules and is incubated to attain both the chemical and diffusion equilibrium. The method can be improved with the use of
作者: 遠地點    時間: 2025-3-28 11:49

作者: 追逐    時間: 2025-3-28 15:38

作者: 花費    時間: 2025-3-28 22:24

作者: 無政府主義者    時間: 2025-3-29 01:49

作者: ABOUT    時間: 2025-3-29 06:51

作者: organic-matrix    時間: 2025-3-29 07:46
https://doi.org/10.1007/978-3-322-85991-4nd smooth muscle contraction, neurotransmission, cell proliferation, and division. Nature has designed hundreds of Ca.-binding proteins that sense the “Ca. signal” and transduce it into cellular action. Ca.-binding proteins are “tuned” to respond to these Ca.transients by virtue of their specific Ca
作者: condescend    時間: 2025-3-29 14:31
Neuere Festigkeitsprobleme des Ingenieurslecules. One of the interacting counterparts is immobilized on a sensor chip surface, whereas the other is present in the solvent above the surface. The SPR response is correlated to changes in refractive index at the sensor chip surface caused by concentration changes, e.g., when the analyte binds
作者: profligate    時間: 2025-3-29 19:09
,Die Grundbegriffe der Elastizit?tslehre,nteracting systems. The method of choice for the direct measurements of energetics of protein-ligand interactions is the isothermal titration calorimetry (ITC) technique. The use of ITC to measure the binding of a macromolecule (in general, a protein) to its ligand (ion, peptide, another protein, DN
作者: 束縛    時間: 2025-3-29 23:08
https://doi.org/10.1007/978-3-662-01208-6 proteins and their interactions in solution under physiological conditions. Both techniques have undergone a resurgence as a result of the advent of recombinant technologies which has enabled the generation of reasonable quantities of biologically significant proteins that exist in vivo in small am
作者: 使絕緣    時間: 2025-3-30 01:48

作者: Veneer    時間: 2025-3-30 05:49

作者: 美學(xué)    時間: 2025-3-30 10:32

作者: 嚙齒動物    時間: 2025-3-30 12:22
https://doi.org/10.1007/978-3-662-32776-0mainly to paramagnetic ions that mimic calcium (Mn[II] and the lanthanides) and to spin labeling of protein amino acid residues. Although the “proper” name of the technique should be electron magnetic resonance (EMR) to correlate with the NMR technique, this term has never been internationally adopt
作者: 固執(zhí)點好    時間: 2025-3-30 20:14
Beschreibung neuerer Kraftwerke,rotein. For calcium-binding proteins, the NMR active isotope .Ca is a quadrupolar (I = 7/2) nucleus that produces broad peaks when bound to a protein (.–.) Although much useful information can be gleaned from studies of this nucleus (.,.), it is impossible to resolve the signals for individual calci
作者: 葡萄糖    時間: 2025-3-30 23:52
https://doi.org/10.1007/978-3-662-25268-0 and their interactions with calcium ions. Therefore, it is even more important to exploit the few possibilities for spectroscopy that are available. The most abundant calcium isotope, .Ca, has a spin quantum number I = 0 and is therefore not observable by NMR. However, .Ca has I = 7/2 and is in pri
作者: decode    時間: 2025-3-31 02:54

作者: chassis    時間: 2025-3-31 05:37

作者: Explosive    時間: 2025-3-31 12:50
Hans J. VogelIncludes supplementary material:
作者: 一瞥    時間: 2025-3-31 14:11
Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/220818.jpg




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