標(biāo)題: Titlebook: Calcium signalling; Approaches and Findi Lars Kaestner Book 2013 Springer-Verlag Berlin Heidelberg 2013 calcium signalling.cardiac myocytes [打印本頁] 作者: Wilson 時(shí)間: 2025-3-21 16:17
書目名稱Calcium signalling影響因子(影響力)
書目名稱Calcium signalling影響因子(影響力)學(xué)科排名
書目名稱Calcium signalling網(wǎng)絡(luò)公開度
書目名稱Calcium signalling網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Calcium signalling被引頻次
書目名稱Calcium signalling被引頻次學(xué)科排名
書目名稱Calcium signalling年度引用
書目名稱Calcium signalling年度引用學(xué)科排名
書目名稱Calcium signalling讀者反饋
書目名稱Calcium signalling讀者反饋學(xué)科排名
作者: OUTRE 時(shí)間: 2025-3-21 21:46
http://image.papertrans.cn/c/image/220811.jpg作者: 包租車船 時(shí)間: 2025-3-22 03:13 作者: 考古學(xué) 時(shí)間: 2025-3-22 07:53 作者: Permanent 時(shí)間: 2025-3-22 09:10 作者: anthropologist 時(shí)間: 2025-3-22 15:03
https://doi.org/10.1007/978-3-8350-9682-0covered the essential role of calcium in the process that is now named excitation–contraction coupling (ECC). Ringer performed experiments with tap water and distilled water and identified the presence of calcium as a necessary requirement for ECC. This early experiments hinted that the physiologica作者: anthropologist 時(shí)間: 2025-3-22 17:48
https://doi.org/10.1007/978-3-8350-9682-0ted with relatively minimal disturbance. Fluorescence-based methods can deliver the following information: (i) a high temporal resolution, down to the order of milliseconds; (ii) spatial resolution down to diffraction-limited spots, and in the future, potentially even lower. The sensor itself can im作者: 朋黨派系 時(shí)間: 2025-3-22 23:29
https://doi.org/10.1007/978-3-8350-9682-0plex (GFP, green fluorescent protein) that naturally occurs in the jellyfish .. Interestingly, the famous paper by Shimomura that identified GFP as a protein only mentioned GFP in one sentence because the paper was primarily dealing with luminescent aequorin. Because the operating principles and mea作者: 沐浴 時(shí)間: 2025-3-23 04:46 作者: 枕墊 時(shí)間: 2025-3-23 05:53
https://doi.org/10.1007/978-3-8350-9682-0d above), there are many techniques that can be used to identify an unknown player. This biochemical and molecular biology-based methodology is reviewed in other sources. Examples include high-pressure liquid chromatography (HPLC), 2D-gel electrophoresis, mass spectrometry, matrix-assisted laser des作者: NAV 時(shí)間: 2025-3-23 10:23
https://doi.org/10.1007/978-3-662-32527-8 the application of a calcium ionophore, such as A23187or ionomycin, to provoke an intracellular situation similar to the digital approach discussed in chapter 2 - Direct evidence - the digital approach. An even more elegant approach is to induce a transient disturbance in cardiomyocytes, the applic作者: 額外的事 時(shí)間: 2025-3-23 17:29 作者: 臨時(shí)抱佛腳 時(shí)間: 2025-3-23 22:01 作者: 豐滿有漂亮 時(shí)間: 2025-3-24 00:11
Grundsatz der Entgeltgleichheit im AGG,alcium signalling has not been written. Although certain effects of calcium in red blood cells have been known for more than 50 years, the means by which calcium enters the red blood cells still has not been completely elucidated. However, what has been clear for quite some time is how the calcium i作者: 形狀 時(shí)間: 2025-3-24 03:44 作者: Temporal-Lobe 時(shí)間: 2025-3-24 10:14
https://doi.org/10.1007/978-3-662-57449-2is far from being completely understood. Therefore further basic research is necessary. With recent developments of optical technologies novel tools to investigate cardiac physiology and pathophysiology became available. They comprise non-linear imaging techniques such as second harmonic generation 作者: medium 時(shí)間: 2025-3-24 11:12 作者: Crumple 時(shí)間: 2025-3-24 16:24
https://doi.org/10.1007/978-3-322-94190-9ent screens. This starts with an optimised culture of human adult cardiomyocytes, allowing culture with diminished dedifferentiation for one week. To allow fluorescence based molecular imaging genetically encoded biosensors need to be expressed in the cardiomyocytes. For transduction of end-differen作者: 多產(chǎn)子 時(shí)間: 2025-3-24 21:54 作者: capsaicin 時(shí)間: 2025-3-25 03:10
https://doi.org/10.1007/978-3-322-85987-7matic because of the autofluorescence or absorption properties of the intracellular milieu. Here, we describe experiments to assess the usability of popular calcium indicators such as Fura-2, Indo-1 and Fluo-4. In our experiments, Fluo-4 turned out to be the preferable indicator because (i) its exci作者: Scintillations 時(shí)間: 2025-3-25 04:15
https://doi.org/10.1007/978-3-8350-9682-0esses ranging from cell proliferation to cell suicide. Life is based on millions of such processes, which can be competitive with, independent of or synergistic with other processes, and builds a network that results in physiological function.作者: 植物茂盛 時(shí)間: 2025-3-25 11:19
Auf dem Weg zur lernenden Organisation,ium buffers play crucial roles, and other calcium transport proteins, such as inositol triphosphate (IP.)-receptors, transient receptor potential (TRP)-channels and .-(NMDA) receptors, just to name a few, have a further modulatory function. 作者: 新手 時(shí)間: 2025-3-25 11:45 作者: 擦試不掉 時(shí)間: 2025-3-25 16:19 作者: defuse 時(shí)間: 2025-3-25 20:48 作者: Nostalgia 時(shí)間: 2025-3-26 04:06
Calcium signalling in cardiac myocytesium buffers play crucial roles, and other calcium transport proteins, such as inositol triphosphate (IP.)-receptors, transient receptor potential (TRP)-channels and .-(NMDA) receptors, just to name a few, have a further modulatory function. 作者: PALL 時(shí)間: 2025-3-26 08:09 作者: 骨 時(shí)間: 2025-3-26 11:44 作者: 單調(diào)女 時(shí)間: 2025-3-26 16:25
Fluorescence-based visualisationprove the spatial resolution by being targeted to particular organelles, cells or organs; (iii) quantitative information once the sensor (preferentially, but not exclusively, a ratiometric sensor) is calibrated.作者: 厭惡 時(shí)間: 2025-3-26 20:29 作者: 未完成 時(shí)間: 2025-3-26 21:41
https://doi.org/10.1007/978-3-8350-9682-0prove the spatial resolution by being targeted to particular organelles, cells or organs; (iii) quantitative information once the sensor (preferentially, but not exclusively, a ratiometric sensor) is calibrated.作者: Parallel 時(shí)間: 2025-3-27 02:58
Grundsatz der Entgeltgleichheit im AGG, that are mediated by calcium as a second messenger range from proliferation signals to the induction of apoptosis. Accordingly, the questions of how these signals are modulated, read by the cell and differentiated by the cell will be a general focus in calcium signalling-related research.作者: isotope 時(shí)間: 2025-3-27 05:51 作者: 阻撓 時(shí)間: 2025-3-27 12:19
https://doi.org/10.1007/978-3-322-94190-9 Example measurements of calcium transients were performed by wide-field video imaging. We discuss the potential application of these cellular and molecular tools in basic research, cardiac safety screens and personalised diagnostics.作者: 涂掉 時(shí)間: 2025-3-27 17:01 作者: 皮薩 時(shí)間: 2025-3-27 19:17
Includes supplementary material: The book combines general concepts and methods to investigate calcium signalling in cells ranging from molecular biology approaches to manipulation of calcium in living cells. The focus within these methods in on the broad range of fluorescence imaging technology, in作者: prosperity 時(shí)間: 2025-3-27 23:49
https://doi.org/10.1007/978-3-8350-9682-0ter and distilled water and identified the presence of calcium as a necessary requirement for ECC. This early experiments hinted that the physiologically relevant calcium concentration is rather small and is in the μM range.作者: canonical 時(shí)間: 2025-3-28 02:16
https://doi.org/10.1007/978-3-8350-9682-0protein only mentioned GFP in one sentence because the paper was primarily dealing with luminescent aequorin. Because the operating principles and measurement techniques are different from those of fluorescent probes, they are discussed in their own chapter. 作者: 都相信我的話 時(shí)間: 2025-3-28 08:01
https://doi.org/10.1007/978-3-8350-9682-0ansmembrane transport can be stopped using low temperature; the cells were washed and lysed, and the radioactivity was measured using an appropriate device, such as a Coulter counter. Thus, the influx can be determined. 作者: 心痛 時(shí)間: 2025-3-28 13:33 作者: 窩轉(zhuǎn)脊椎動(dòng)物 時(shí)間: 2025-3-28 14:34 作者: STERN 時(shí)間: 2025-3-28 20:11
Neue Wege zur Wassersterilisierung (Katadyn)odulation in calcium. This concept is a very complex approach because function can be modulated by many parameters that are directly or indirectly influenced by calcium. Therefore, primary and secondary effects are difficult or impossible to discriminate.作者: 露天歷史劇 時(shí)間: 2025-3-29 02:30
Direct evidence - the digital approachter and distilled water and identified the presence of calcium as a necessary requirement for ECC. This early experiments hinted that the physiologically relevant calcium concentration is rather small and is in the μM range.作者: Finasteride 時(shí)間: 2025-3-29 03:27 作者: 繁榮中國(guó) 時(shí)間: 2025-3-29 11:12 作者: CERE 時(shí)間: 2025-3-29 13:12
Molecular biology based approachesed in other sources. Examples include high-pressure liquid chromatography (HPLC), 2D-gel electrophoresis, mass spectrometry, matrix-assisted laser desorption/ionisation in combination with time-of-flight mass spectrometer (MALDI-TOF) and expression analysis with DNA chips or next-generation sequencing. 作者: 持久 時(shí)間: 2025-3-29 15:45
Manipulation of calciumn chapter 2 - Direct evidence - the digital approach. An even more elegant approach is to induce a transient disturbance in cardiomyocytes, the application of 20 mM caffeine leads to the opening of the internal calcium stores, namely the sarcoplasmic reticulum (SR), by opening the ryanodine receptors. 作者: 裂縫 時(shí)間: 2025-3-29 20:38 作者: Defraud 時(shí)間: 2025-3-30 02:49
Book 2013 calcium in living cells. The focus within these methods in on the broad range of fluorescence imaging technology, in particular on optical sectioning techniques and fast image acquisition. In addition to these general guidelines there are application examples in a context beyond calcium signalling 作者: 平常 時(shí)間: 2025-3-30 06:31 作者: 軟膏 時(shí)間: 2025-3-30 11:26
A primary culture system for sustained expression of a calcium sensor in preserved adult rat ventric medium supplemented with an insulin–transferrin–selenite mixture on substrates coated with extracellular matrix proteins showed an increased cell attachment and a conserved cross-striation. Moreover, these myocytes displayed optimised preservation of their contractile behaviour and Ca. signalling e作者: Axillary 時(shí)間: 2025-3-30 12:39 作者: affect 時(shí)間: 2025-3-30 18:00 作者: 共同時(shí)代 時(shí)間: 2025-3-31 00:39 作者: 付出 時(shí)間: 2025-3-31 03:27
https://doi.org/10.1007/978-3-322-85987-7 medium supplemented with an insulin–transferrin–selenite mixture on substrates coated with extracellular matrix proteins showed an increased cell attachment and a conserved cross-striation. Moreover, these myocytes displayed optimised preservation of their contractile behaviour and Ca. signalling e作者: SNEER 時(shí)間: 2025-3-31 08:22
https://doi.org/10.1007/978-3-322-85987-7lation by prostaglandin E. and lysophosphatidic acid. For the latter stimulus, we recorded emission spectra of individual erythrocytes to confirm largely unaltered Fluo-4 emission. Our results emphasise that in erythrocytes measurements of intracellular calcium are reliably possible with Fluo-4 and 作者: Migratory 時(shí)間: 2025-3-31 09:45
Introductionminute amounts of calcium were necessary to maintain heart muscle contractility. Currently, approximately 130 years later, our knowledge of calcium as a versatile signalling molecule has increased tremendously, and we have a good understanding of its general principles. As described in these reviews
