標題: Titlebook: Calcium Signaling Protocols; David G. Lambert Book 19991st edition Springer Science+Business Media New York 1999 [打印本頁] 作者: tricuspid-valve 時間: 2025-3-21 17:31
書目名稱Calcium Signaling Protocols影響因子(影響力)
作者: 制造 時間: 2025-3-21 23:19
https://doi.org/10.1007/978-3-658-33012-5An elevation in intracellular calcium concentration ([Ca.].) acts to trigger a range of cellular events including neurotransmitter release, muscle contraction, and oocyte fertilization (.,.). The pattern of elevation in [Ca.]. and response to that elevation is dependent on the agonist and the cell type.作者: Gum-Disease 時間: 2025-3-22 03:01
Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2An elevation in intracellular calcium concentration ([Ca.].) acts to trigger a range of cellular events including neurotransmitter release, muscle contraction, and oocyte fertilization (.,.). The pattern of elevation in [Ca.]. and response to that elevation is dependent on the agonist and the cell type.作者: Glower 時間: 2025-3-22 04:34
Springer Science+Business Media New York 1999作者: DEFER 時間: 2025-3-22 09:57 作者: Visual-Field 時間: 2025-3-22 13:34
Realit?tsbezüge im Mathematikunterrichtven the wide diversity of fluorescent Ca. indicators that are now available, the variety of cells to be investigated, and an increasing range of detection equipment that can be used. Therefore, this chapter is designed to provide the user with sufficient background in the technology so that he or sh作者: Visual-Field 時間: 2025-3-22 18:37 作者: Decrepit 時間: 2025-3-22 22:19 作者: 恃強凌弱的人 時間: 2025-3-23 01:48
Helmuth Harig,Christian J. Langenbachal importance. To determine Ca.-related cellular dynamics accurately, it is necessary to measure three-dimensionally resolved [Ca.]. with sufficient temporal resolution to follow fast cellular responses that generate signal pulses and wave propagation. Several techniques have been developed to assay作者: 變量 時間: 2025-3-23 09:25
Neue Materialien für innovative Produkter cell parameters, but the usefulness of this approach is limited by several problems, not the least of which is temporal averaging. In population studies, agonists often evoke a characteristic peak and plateau type of response irrespective of the stimulus intensity. However, equivalent experiments 作者: 獨輪車 時間: 2025-3-23 10:52 作者: 抒情短詩 時間: 2025-3-23 15:42
,?Neue Medien ‘— Was ist das eigentlich?,tudies was performed by Hodgkin and Keynes (.) to measure the rate at which .Ca. labeled with .Ca. crosses the surface membrane of resting squid axons, or during nervous activity. In a few large excitable cells, it was also possible to measure Ca. entry indirectly through the measurement of inward c作者: 永久 時間: 2025-3-23 19:28
Gerhard Strube,Christoph H?lscherembrane down the steep concentration gradient (.–.). Ca. can enter through two main classes of plasma membrane-located Ca. channels: receptor operated and voltage sensitive (.–.). Voltage-sensitive Ca. channels are involved in a wide and diverse array of physiological responses including neurotransm作者: 頌揚國家 時間: 2025-3-24 01:57
G. Günter Vo?,Werner Holly,Klaus Boehnkeing inositol phospholipid-specific phospholipase C (PLC). Activated PLC catalyzes the hydrolysis of phosphatidylinositol bisphosphate (PIP.), a minor membrane phospholipid, to form two second messengers, diacylglycerol (DAG) and inositol (1,4,5)trisphosphate [Ins(1,4,5,)P.]. DAG is a direct activato作者: 慷慨援助 時間: 2025-3-24 03:40 作者: 咒語 時間: 2025-3-24 06:31
Neue Medien und kindliche Entwicklungroduce the second messengers, inositol(1,4,5)trisphosphate [Ins(1,4,5)P.] and diacylglycerol (.). Ins(1,4,5)P. interacts with specific receptor populations of ligand-gated channels to mobilize nonmitochondrial intracellular calcium (Ca.) stores (.). Because Ins(1,4,5)P. is very hydrophilic, it canno作者: Hectic 時間: 2025-3-24 14:35 作者: shrill 時間: 2025-3-24 18:02 作者: Conspiracy 時間: 2025-3-24 20:53 作者: 詞匯 時間: 2025-3-25 00:47 作者: APEX 時間: 2025-3-25 05:33 作者: 入會 時間: 2025-3-25 09:55
Measurement of Inositol (Poly)phosphate Formation Using [3H]Inositol Labeling Protocols in Permeabilr of protein kinase C isozymes, Ins(1,4,5)P. mobilizes intracellular Ca.. G protein-coupled receptors couple to the PLC-β family via G proteins, and tyrosine kinase receptors activate PLCγ isozymes (.). Regardless of the PLC isozyme activated, the product is invariantly Ins(1,4,5)P..作者: 注射器 時間: 2025-3-25 14:43
Helmuth Harig,Christian J. Langenbach [Ca.]., but a revolution in the study of intracellular [Ca.]. occurred when fluorescent dyes for Ca. were developed (.). Since then, fluorescent dyes and fluorescent microscopy have been used to observe resting and nonresting [Ca.] in intact cells as well as in subcellular fractions.作者: AFFIX 時間: 2025-3-25 17:55
Neue Materialien — Trends und Entwicklungen current knowledge of cellular calcium homeostasis (.). Typically, the fluorescent signal has been monitored using cuvet-based fluorometers or by confocal fluorescent microscopy. Although acceptable for a number of applications, these methods are relatively labor intensive and are not suitable for screening large numbers of compounds.作者: 不透明性 時間: 2025-3-25 21:30
G. Günter Vo?,Werner Holly,Klaus Boehnker of protein kinase C isozymes, Ins(1,4,5)P. mobilizes intracellular Ca.. G protein-coupled receptors couple to the PLC-β family via G proteins, and tyrosine kinase receptors activate PLCγ isozymes (.). Regardless of the PLC isozyme activated, the product is invariantly Ins(1,4,5)P..作者: 鑒賞家 時間: 2025-3-26 01:13 作者: PRISE 時間: 2025-3-26 06:06 作者: Kidnap 時間: 2025-3-26 11:49
Realit?tsbezüge im Mathematikunterrichttion equipment that can be used. Therefore, this chapter is designed to provide the user with sufficient background in the technology so that he or she can move toward developing a protocol that will suit the cells, the experimental objectives, and the equipment available to the investigator.作者: happiness 時間: 2025-3-26 15:03
Gerhard Strube,Christoph H?lscher and voltage sensitive (.–.). Voltage-sensitive Ca. channels are involved in a wide and diverse array of physiological responses including neurotransmitter release (.). In addition, many guanine nucleotide protein (G) coupled receptors couple to voltage-sensitive Ca. channels to reduce Ca. influx (.,.).作者: 心胸狹窄 時間: 2025-3-26 16:57
Fluorescent Measurement of [Ca2+]ction equipment that can be used. Therefore, this chapter is designed to provide the user with sufficient background in the technology so that he or she can move toward developing a protocol that will suit the cells, the experimental objectives, and the equipment available to the investigator.作者: BULLY 時間: 2025-3-26 23:20
Measurement of [3H]PN200-110 and [125I]ω-Conotoxin MVIIA Binding and voltage sensitive (.–.). Voltage-sensitive Ca. channels are involved in a wide and diverse array of physiological responses including neurotransmitter release (.). In addition, many guanine nucleotide protein (G) coupled receptors couple to voltage-sensitive Ca. channels to reduce Ca. influx (.,.).作者: 拍下盜公款 時間: 2025-3-27 04:44 作者: Freeze 時間: 2025-3-27 08:47
Realit?tsbezüge im Mathematikunterrichtally dyes that respond to Ca. with shifts of excitation or emission spectra (.–.). By measuring at two selected wavelengths (either dual emission or dual excitation), it is possible to calculate the proportion of dye in the Ca.-bound and Ca.-free forms.作者: Cocker 時間: 2025-3-27 11:27 作者: 捏造 時間: 2025-3-27 14:01
Neue Materialien für innovative Produktehese patterns cannot be observed in population studies since cells oscillate out of phase and at different frequencies, resulting in a smooth population average. It is only when cells are electrically well coupled that synchronized, population oscillations can be recorded, but this case is more the exception than the rule.作者: needle 時間: 2025-3-27 21:21
https://doi.org/10.1007/978-3-662-39669-8 single-channel characteristics of the Ins(1,4,5)P. receptor (such as channel unit conductance and open and closed times) in artificial planar lipid bilayers and relating this information to that obtained from various other studies.作者: Lipoprotein 時間: 2025-3-28 01:43
Measurement of [Ca2+]i in Cell Suspensions Using Indo-1ally dyes that respond to Ca. with shifts of excitation or emission spectra (.–.). By measuring at two selected wavelengths (either dual emission or dual excitation), it is possible to calculate the proportion of dye in the Ca.-bound and Ca.-free forms.作者: 完整 時間: 2025-3-28 03:57
Confocal Microscopye a few examples of basic preparation techniques for defining structure and localizing target molecules in cells. As one example, however, we will refer to our simple imaging technique for studying the three-dimensional (3D) microscopic distribution of calcium during skeletogenesis in the mammalian fetus.作者: Innocence 時間: 2025-3-28 09:58 作者: aggravate 時間: 2025-3-28 14:23 作者: assent 時間: 2025-3-28 18:27 作者: CALL 時間: 2025-3-28 21:26 作者: STENT 時間: 2025-3-29 01:49
Measurement of Inositol(1,4,5)trisphosphate Using a Stereospecific Radioreceptor Mass AssayP. receptor on the smooth endoplasmic reticulum, causing a conformational change that opens the intrinsic calcium channel in the receptor, thus allowing the efflux of calcium ions from the intracellular stores (.). For further details, see the reviews by Berridge (.) and Furuichi and Mikoshiba (.).作者: 遠足 時間: 2025-3-29 05:19
Measurement of Calcium Fluxes in Permeabilized Cells Using a 45Ca2+ Uptake and Release Assay,5)P.” (.). An alternative approach involves disruption of the plasma membrane and preparation of microsomes from the intracellular vesicular Ca. stores (.,.), however, these preparations exhibit a loss of Ins(1,4,5)P. responsiveness compared to cells. The author will describe a .Ca.-release assay u作者: 連鎖 時間: 2025-3-29 10:05 作者: 正面 時間: 2025-3-29 13:42 作者: OFF 時間: 2025-3-29 16:58 作者: 可能性 時間: 2025-3-29 22:54 作者: irreducible 時間: 2025-3-30 00:45 作者: 挑剔為人 時間: 2025-3-30 05:14 作者: DIS 時間: 2025-3-30 08:32 作者: FLINT 時間: 2025-3-30 13:10 作者: jumble 時間: 2025-3-30 20:28
Measurement of [Ca2+]i in Cell Suspensions Using Indo-1mulation of receptors, belonging to almost all the classes so far described, will result in changes in [Ca.].. These changes might be directly induced by either Ca.-influx or Ca.-mobilization from intracellular stores, or indirectly by a number of other mechanisms (.–.). The development of fluoresce作者: 受人支配 時間: 2025-3-31 00:35
Confocal Microscopyhis is intended to be a general introduction to the possibilities available, it is not appropriate to include detailed descriptions of the specialized techniques used in physiological studies leading to the imaging and measurement of Ca.-containing samples. Instead, the reader is referred to . in th作者: tendinitis 時間: 2025-3-31 01:27
