作者: 腐爛 時間: 2025-3-22 00:10
Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2bed in this volume. Whole cell suspensions are loaded with Fura-2 and then placed into a cuvette-based fluorimetric system (measuring 510 nm emission at alternating 340/340 nm excitation). Cells can be stimulated with agonists and antagonists to enable temporal response profiling and concentration–r作者: 偏見 時間: 2025-3-22 03:36
Confocal Microscopy: Theory and Applications for Cellular Signalingful approach to examine cellular structure and function. Allied with the development of suitable tools, it is now possible to interrogate a wide range of structural and functional aspects on both fixed and live cells. Here we describe the basic principles underlying confocal microscopy and provide m作者: OVER 時間: 2025-3-22 06:04
Ratiometric Ca2+ Measurements Using the FlexStation?Scanning Fluorometer dual-wavelength fluorescent probes. The FlexStation uses a Xenon flashlamp and monochromators for both excitation and emission light to allow the use of a wide range of fluorescent indicators. The system incorporates a fluid transfer system for addition of test compounds from a source plate to the 作者: 駁船 時間: 2025-3-22 12:21
Measuring Ca2+ Changes in Multiwell Format Using the Fluorometric Imaging Plate Reader (FLIPR?)onal plate readers is the ability to measure fluorescence emission from multiple wells (96 wells or 384 wells) simultaneously and with high temporal resolution. Consequently, FLIPR has been used extensively to record dynamic intracellular processes such as changes in intracellular Ca. ion concentrat作者: Hla461 時間: 2025-3-22 14:47
Ratiometric [Ca2+]i Measurements in Adherent Cell-Lines Using the NOVOstar Microplate Readere of cellular events. Consequentially, experimental measurement of [Ca.]. is a potent technique for the medical science laboratory. The NOVOstar microplate reader is a versatile system, which may be easily configured to measure [Ca.].. Moreover, the relatively low cost of this system makes it an att作者: Hla461 時間: 2025-3-22 17:37
Whole-Cell Patch-Clamp Recording of Voltage-Sensitive Ca2+ Channel Currents in Single Cells: Heterolle cells, heart pacemaker tissue and endocrine cells. Whole-cell recording is a functional electrophysiological assay that allows real-time measurement of macroscopic VSCC activity at the level of single cells. Using this technique, it is possible to probe the molecular physiology, pharmacology, and作者: 植物茂盛 時間: 2025-3-22 23:16 作者: 故意釣到白楊 時間: 2025-3-23 03:10 作者: 冷漠 時間: 2025-3-23 05:56 作者: CAGE 時間: 2025-3-23 09:54 作者: Vital-Signs 時間: 2025-3-23 15:37 作者: PAC 時間: 2025-3-23 20:45 作者: ANT 時間: 2025-3-23 22:42
Measurement of Changes in Endothelial and Smooth Muscle Ca2+ in Pressurized Arterieshas provided information linking Ca. events to changes in arterial function. Here we describe the in vitro techniques used to selectively load Ca. indicators into either the endothelium or the smooth muscle of cannulated rat cremaster arteries. These vessels normally develop spontaneous myogenic ton作者: Anthropoid 時間: 2025-3-24 04:39 作者: GLIB 時間: 2025-3-24 09:59
Neue Konzepte für das Kostenmanagementoteins by immunocytochemistry; cytoplasmic and organelle Ca. signaling using fluorescent indicators; second messenger generation using fluorescently tagged biosensors; and ligand/receptor internalization using fluorescently tagged peptide agonists and receptors.作者: 過份好問 時間: 2025-3-24 13:32 作者: allude 時間: 2025-3-24 16:37 作者: Efflorescent 時間: 2025-3-24 19:23
Fluorescent Measurement of [Ca2+]c: Basic Practical Considerationso take great care in this process. This chapter describes the theory of these processes and some of the pitfalls users should be aware of. Precise experimental details can be found in the subsequent chapters of this volume.作者: Corral 時間: 2025-3-25 00:35
Confocal Microscopy: Theory and Applications for Cellular Signalingoteins by immunocytochemistry; cytoplasmic and organelle Ca. signaling using fluorescent indicators; second messenger generation using fluorescently tagged biosensors; and ligand/receptor internalization using fluorescently tagged peptide agonists and receptors.作者: omnibus 時間: 2025-3-25 06:54 作者: 可以任性 時間: 2025-3-25 11:15 作者: resilience 時間: 2025-3-25 11:52 作者: Jogging 時間: 2025-3-25 19:44 作者: 助記 時間: 2025-3-25 21:08
Zeitschrift für Betriebswirtschaft of contractile function and cell signalling. Here the loading of cells either with an esterified fluorescence indicator prior to patch clamp recording, or dye loading via the patch pipette with “free” indicator, is described to allow simultaneous measurement of fluorescence and electrical signals.作者: 顯赫的人 時間: 2025-3-26 03:00 作者: 軍火 時間: 2025-3-26 06:52
Combined Calcium Fluorescence Recording with Ionic Currents in Contractile Cells of contractile function and cell signalling. Here the loading of cells either with an esterified fluorescence indicator prior to patch clamp recording, or dye loading via the patch pipette with “free” indicator, is described to allow simultaneous measurement of fluorescence and electrical signals.作者: forestry 時間: 2025-3-26 09:59 作者: SCORE 時間: 2025-3-26 14:39
Measurement of Inositol(1,4,5)Trisphosphate Using a Stereospecific Radioreceptor Mass Assayis is mixed with [.H]-labeled and unlabeled (generated from biological samples or standards) Ins(1,4,5)P.. Using the same principles as for radioimmunoassay/ELISA the mass of Ins(1,4,5)P. in biological samples can be estimated.作者: SUE 時間: 2025-3-26 19:00 作者: DALLY 時間: 2025-3-26 21:00
neue betriebswirtschaftliche forschung (nbf)erature and 45°C. The FlexStation can be configured to read a range of plate sizes. In this chapter generic methods for assessing intracellular Ca. on the FlexStation using ratiometric dyes are described.作者: AGGER 時間: 2025-3-27 04:08
https://doi.org/10.1007/978-3-642-85128-5g or damage, and that the fluorescent indicator-loaded vessels behave similarly to unloaded preparations. Importantly, these approaches are applicable to both isobaric and isometric preparations and have been also used for the study of a number of vascular beds including cerebral, mesenteric, coronary, and skeletal muscle vasculatures.作者: Prophylaxis 時間: 2025-3-27 07:50
Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2ceptors and channels. Loading characteristics and the need for agents to retain loaded dye (e.g., probenecid) need to be determined empirically. Calibration of whole cell suspensions to convert the fluorescent signal into Ca. is simply performed using Triton-X lysis (to determine ..) and EGTA chelation (to determine ..).作者: 人類學(xué)家 時間: 2025-3-27 12:53
Ratiometric Ca2+ Measurements Using the FlexStation?Scanning Fluorometererature and 45°C. The FlexStation can be configured to read a range of plate sizes. In this chapter generic methods for assessing intracellular Ca. on the FlexStation using ratiometric dyes are described.作者: Inculcate 時間: 2025-3-27 15:31
Measurement of Changes in Endothelial and Smooth Muscle Ca2+ in Pressurized Arteriesg or damage, and that the fluorescent indicator-loaded vessels behave similarly to unloaded preparations. Importantly, these approaches are applicable to both isobaric and isometric preparations and have been also used for the study of a number of vascular beds including cerebral, mesenteric, coronary, and skeletal muscle vasculatures.作者: Fibrin 時間: 2025-3-27 19:34 作者: Self-Help-Group 時間: 2025-3-27 22:57 作者: consolidate 時間: 2025-3-28 04:08
Neue Konzepte für das Kostenmanagementbed in this volume. Whole cell suspensions are loaded with Fura-2 and then placed into a cuvette-based fluorimetric system (measuring 510 nm emission at alternating 340/340 nm excitation). Cells can be stimulated with agonists and antagonists to enable temporal response profiling and concentration–r作者: 蘑菇 時間: 2025-3-28 06:39
Neue Konzepte für das Kostenmanagementful approach to examine cellular structure and function. Allied with the development of suitable tools, it is now possible to interrogate a wide range of structural and functional aspects on both fixed and live cells. Here we describe the basic principles underlying confocal microscopy and provide m作者: RAFF 時間: 2025-3-28 13:12
neue betriebswirtschaftliche forschung (nbf) dual-wavelength fluorescent probes. The FlexStation uses a Xenon flashlamp and monochromators for both excitation and emission light to allow the use of a wide range of fluorescent indicators. The system incorporates a fluid transfer system for addition of test compounds from a source plate to the 作者: MILK 時間: 2025-3-28 15:17 作者: 埋伏 時間: 2025-3-28 20:07
Frank Feldmann,Kai-Uwe Hellmanne of cellular events. Consequentially, experimental measurement of [Ca.]. is a potent technique for the medical science laboratory. The NOVOstar microplate reader is a versatile system, which may be easily configured to measure [Ca.].. Moreover, the relatively low cost of this system makes it an att作者: 減至最低 時間: 2025-3-29 00:24
Neue Konzeptionen für das Wohnen im Alterle cells, heart pacemaker tissue and endocrine cells. Whole-cell recording is a functional electrophysiological assay that allows real-time measurement of macroscopic VSCC activity at the level of single cells. Using this technique, it is possible to probe the molecular physiology, pharmacology, and作者: 阻撓 時間: 2025-3-29 04:27 作者: Musculoskeletal 時間: 2025-3-29 08:07 作者: creatine-kinase 時間: 2025-3-29 12:30
Schriften des Institut Arbeit und Technikzymes of the phospholipase C (PLC) family. Binding of IP. to its cognate receptor in the endoplasmic reticulum membrane leads to release of Ca. into the cytoplasm, which is involved in the regulation of an array of cellular functions. Traditional techniques for the detection of IP. have required the作者: 縫紉 時間: 2025-3-29 16:07 作者: nullify 時間: 2025-3-29 22:07 作者: mastoid-bone 時間: 2025-3-30 01:01 作者: laparoscopy 時間: 2025-3-30 04:36
https://doi.org/10.1007/978-3-642-85128-5has provided information linking Ca. events to changes in arterial function. Here we describe the in vitro techniques used to selectively load Ca. indicators into either the endothelium or the smooth muscle of cannulated rat cremaster arteries. These vessels normally develop spontaneous myogenic ton作者: Glutinous 時間: 2025-3-30 09:06 作者: 蓋他為秘密 時間: 2025-3-30 12:42
https://doi.org/10.1007/978-3-658-20840-0ded from multiple locations using imaging devices and organic dyes or genetic probe (Tallini et al. PNAS 103(12):4753–4758, 2006) from whole heart. Here, we describe the optical apparatus and the method to record intracellular calcium transients.
