標(biāo)題: Titlebook: CRISPR Guide RNA Design; Methods and Protocol Tudor A. Fulga,David J. H. F. Knapp,Quentin R. V. Book 2021 Springer Science+Business Media, [打印本頁] 作者: bile-acids 時(shí)間: 2025-3-21 20:05
書目名稱CRISPR Guide RNA Design影響因子(影響力)
作者: 隱士 時(shí)間: 2025-3-22 00:07 作者: anarchist 時(shí)間: 2025-3-22 01:20
978-1-0716-0689-6Springer Science+Business Media, LLC, part of Springer Nature 2021作者: Jingoism 時(shí)間: 2025-3-22 05:30
Tudor A. Fulga,David J. H. F. Knapp,Quentin R. V. Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts作者: adumbrate 時(shí)間: 2025-3-22 11:22 作者: 委托 時(shí)間: 2025-3-22 16:15
https://doi.org/10.1057/9780230245099onsiderations for the design of short guide (sg) RNAs include the assessment of possible off-target activities, the prediction of on-target efficacies and mutational outcome. Manual design of sgRNAs taking into account these parameters, however, remains a difficult task. Thus, computational tools to作者: 委托 時(shí)間: 2025-3-22 19:46
https://doi.org/10.1057/9780230245099ine, and gene therapy. However, CRISPR nucleases can cleave off-target sites as well as on-target sites, causing unwanted mutations. Furthermore, after CRISPR treatments are delivered into cells or organisms, it is important to estimate the resulting mutation rates and to determine the patterns of m作者: Grating 時(shí)間: 2025-3-22 23:57 作者: FRONT 時(shí)間: 2025-3-23 01:49
The Thin Filament in Insect Flight Muscleing promise as a therapeutic for patients with genetic diseases. Multiple different CRISPR systems have been identified, each with its own target DNA recognition sequence, expanding the editable mammalian genome. It is also now appreciated that chemically modified nucleic acids can substitute for un作者: 脫毛 時(shí)間: 2025-3-23 06:42
The Thin Filament in Insect Flight Muscleby enzymatic reactions, however the process becomes time-consuming as the number of sgRNAs increases and does not allow the incorporation of chemical modifications that can improve or expand the functionality of CRISPR. Solid-phase RNA synthesis can overcome these issues, but highly pure full-length作者: gorgeous 時(shí)間: 2025-3-23 09:44 作者: 整潔 時(shí)間: 2025-3-23 16:42 作者: Exuberance 時(shí)間: 2025-3-23 20:21 作者: 天氣 時(shí)間: 2025-3-23 23:03 作者: 闡釋 時(shí)間: 2025-3-24 03:21
https://doi.org/10.1007/978-3-642-99611-5rganisms. Known as the CRISPR-Cas system, Cas endonucleases such as Cas9 and Cas12a (also known as Cpf1) and guide RNA (gRNA) complexes recognize and cleave the target DNA, allowing for targeted gene manipulation. Along with the Cas protein engineering, gRNA engineering has broadened the application作者: 薄荷醇 時(shí)間: 2025-3-24 09:16 作者: anniversary 時(shí)間: 2025-3-24 13:35 作者: 惰性女人 時(shí)間: 2025-3-24 18:12
,Schichtgesteine sind Geschichtsbücher,s first subjected to digestion by CRISPR-Cas9 in vitro and then to whole genome sequencing, which results in unusual patterns of straight alignments at on-target and potential off-target sites. Analysis of these data with the Digenome-seq computer program allows for identification of the in vitro cl作者: 使激動(dòng) 時(shí)間: 2025-3-24 21:32
Die Entfaltung der Wirbeltiere,ion activator-like effector nucleases (TALENs) show great promises for research and therapeutic applications. One major concern is the off-target effects generated by these nucleases at unintended genomic sequences. In silico methods are usually used for off-target site prediction. However, based on作者: Liability 時(shí)間: 2025-3-25 01:34 作者: temperate 時(shí)間: 2025-3-25 05:05
1064-3745 nd Protocols. provides a comprehensive pipeline for guide RNA design and aims to be an invaluable resource in applying this powerful technology to basic research and therapeutic applications..978-1-0716-0689-6978-1-0716-0687-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: avarice 時(shí)間: 2025-3-25 08:38
Cloud-Based Design of Short Guide RNA (sgRNA) Libraries for CRISPR Experimentssage. We will also discuss the installation of a dockerized version of CRISPR Library Designer (CLD) that can be deployed locally or in the cloud to support the end-to-end design of sgRNA libraries for more than 50 different organisms. CLD was built upon E-CRISP to further increase the scope of sgRN作者: 乞丐 時(shí)間: 2025-3-25 14:58 作者: Myocyte 時(shí)間: 2025-3-25 17:00 作者: 尊重 時(shí)間: 2025-3-25 20:19 作者: characteristic 時(shí)間: 2025-3-26 02:04 作者: cyanosis 時(shí)間: 2025-3-26 06:03 作者: Allergic 時(shí)間: 2025-3-26 11:23
https://doi.org/10.1057/9780230245099), a tool for designing CRISPR targets (Cas-Designer), and an assessment tool (Cas-Analyzer). These programs are all implemented on our website so that researchers can easily design CRISPR guide RNAs and assess the resulting mutations by simply clicking on the appropriate buttons; no login process is required.作者: neolith 時(shí)間: 2025-3-26 13:46
Web-Based CRISPR Toolkits: Cas-OFFinder, Cas-Designer, and Cas-Analyzer), a tool for designing CRISPR targets (Cas-Designer), and an assessment tool (Cas-Analyzer). These programs are all implemented on our website so that researchers can easily design CRISPR guide RNAs and assess the resulting mutations by simply clicking on the appropriate buttons; no login process is required.作者: palliative-care 時(shí)間: 2025-3-26 19:59 作者: 包裹 時(shí)間: 2025-3-26 23:52 作者: 個(gè)人長篇演說 時(shí)間: 2025-3-27 01:29 作者: disrupt 時(shí)間: 2025-3-27 09:21 作者: 意外 時(shí)間: 2025-3-27 11:28 作者: FIS 時(shí)間: 2025-3-27 15:33 作者: NOTCH 時(shí)間: 2025-3-27 19:16
Harnessing tRNA for Processing Ability and Promoter Activityengineered tRNAs can be used to process gRNAs from Pol-II transcripts, thus enabling spatial/temporal control of gRNA expression. Here we describe the design, cloning, and testing of tRNA scaffolds for both Pol-III-driven expression of different levels of gRNAs, and for processing gRNAs from Pol-II transcripts.作者: amenity 時(shí)間: 2025-3-28 01:02 作者: Endearing 時(shí)間: 2025-3-28 05:10
https://doi.org/10.1007/978-3-662-25288-8the presence of a user defined inducer. In this chapter, I cover the know-how relating to the design and synthesis of iSBH-sgRNAs, as well as the implementation in mammalian cells of inducible CRISPR-TR strategies based on this technology.作者: Incumbent 時(shí)間: 2025-3-28 08:53 作者: 微塵 時(shí)間: 2025-3-28 14:12 作者: Budget 時(shí)間: 2025-3-28 16:05
Controlling the Activity of CRISPR Transcriptional Regulators with Inducible sgRNAsthe presence of a user defined inducer. In this chapter, I cover the know-how relating to the design and synthesis of iSBH-sgRNAs, as well as the implementation in mammalian cells of inducible CRISPR-TR strategies based on this technology.作者: 萬靈丹 時(shí)間: 2025-3-28 19:31
Gene Manipulation Using Fusion Guide RNAs for Cas9 and Cas12as of the CRISPR-Cas system. Recently, we have developed fusion guide RNAs (fgRNAs) for orthogonal gene manipulation using Cas9 and Cas12a. Here, we describe the methods for designing and generating fgRNAs-expression constructs to achieve multiplex genome editing and gene manipulation in human cells.作者: sinoatrial-node 時(shí)間: 2025-3-29 00:07 作者: 激怒某人 時(shí)間: 2025-3-29 06:59 作者: 獨(dú)白 時(shí)間: 2025-3-29 07:37
The Thin Filament in Insect Flight Muscleprovide a detailed protocol for use of CRISPR-Cpf1 and chemically modified guide RNAs in cell lines, outlining the steps from designing guide RNAs to a target gene of interest, delivery and expression in cells, and analysis of gene editing events.作者: cravat 時(shí)間: 2025-3-29 15:29
The Thin Filament in Insect Flight Muscle active sgRNA. The benefits of our approach lie in the stringent purification of the DNA-targeting 20-mer, the reduced synthesis of the constant 79-mer each time a new sgRNA is required, and the rapid access it provides to custom libraries of sgRNAs.作者: heterogeneous 時(shí)間: 2025-3-29 16:10
Noch einmal: Geschichte in Schichtgesteinen,identify off-target cleavage events throughout an entire genome. Together, these techniques can be used to assess the reliability of experimental models generated using CRISPR/Cas9 as well as the safety of therapeutics employing this technology.作者: 過于光澤 時(shí)間: 2025-3-29 21:53 作者: 轎車 時(shí)間: 2025-3-30 00:22 作者: 臭了生氣 時(shí)間: 2025-3-30 07:58 作者: 入伍儀式 時(shí)間: 2025-3-30 09:00 作者: 一加就噴出 時(shí)間: 2025-3-30 16:11
