標(biāo)題: Titlebook: C. elegans; Methods and Applicat Gal Haspel,Anne C. Hart Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under exc [打印本頁(yè)] 作者: Fuctionary 時(shí)間: 2025-3-21 16:12
書目名稱C. elegans影響因子(影響力)
書目名稱C. elegans影響因子(影響力)學(xué)科排名
書目名稱C. elegans網(wǎng)絡(luò)公開(kāi)度
書目名稱C. elegans網(wǎng)絡(luò)公開(kāi)度學(xué)科排名
書目名稱C. elegans被引頻次
書目名稱C. elegans被引頻次學(xué)科排名
書目名稱C. elegans年度引用
書目名稱C. elegans年度引用學(xué)科排名
書目名稱C. elegans讀者反饋
書目名稱C. elegans讀者反饋學(xué)科排名
作者: 楓樹(shù) 時(shí)間: 2025-3-21 21:53
The Process of Developing Microformatsar expansion). The epitope-preserving ExCel protocol enables imaging of endogenous proteins with off-the-shelf antibodies, at a?~?100?nm resolution (~2.8× linear expansion). The iterative ExCel protocol allows readout of fluorescent proteins at ~25?nm resolution (~20× linear expansion). The protocol作者: 寬宏大量 時(shí)間: 2025-3-22 03:03 作者: Ejaculate 時(shí)間: 2025-3-22 07:24
1064-3745 nown pitfalls.?.Authoritative and up-to-date, .C. elegans: Methods and Applications, Third Edition. offers the kind of clear guidance that will serve all researchers working with these vital and dynamic organisms..978-1-0716-2183-7978-1-0716-2181-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 考得 時(shí)間: 2025-3-22 09:57 作者: 無(wú)法取消 時(shí)間: 2025-3-22 14:43 作者: 無(wú)法取消 時(shí)間: 2025-3-22 18:31
Microbial Rhodopsin Optogenetic Tools: Application for Analyses of Synaptic Transmission and of Neutated variants with specificity for cyclic AMP. These can be used to regulate intracellular levels of cGMP and cAMP, which are important second messengers in sensory and other neurons. We further show how they can be combined with cyclic nucleotide-gated channels in two-component optogenetics, for d作者: 誘拐 時(shí)間: 2025-3-22 23:51 作者: 進(jìn)步 時(shí)間: 2025-3-23 05:27 作者: Concerto 時(shí)間: 2025-3-23 07:36
Book 2022Latest editionfalls.?.Authoritative and up-to-date, .C. elegans: Methods and Applications, Third Edition. offers the kind of clear guidance that will serve all researchers working with these vital and dynamic organisms..作者: 引水渠 時(shí)間: 2025-3-23 12:53
https://doi.org/10.1007/978-1-0716-2181-3Caenorhabditis elegans; Nematode; Optogenetics; Genetic tools; Research laboratories作者: PRE 時(shí)間: 2025-3-23 16:23 作者: Camouflage 時(shí)間: 2025-3-23 18:02
Gal Haspel,Anne C. HartIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts作者: 的事物 時(shí)間: 2025-3-24 01:00
Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/220096.jpg作者: gimmick 時(shí)間: 2025-3-24 03:13 作者: Connotation 時(shí)間: 2025-3-24 08:03
Observing and Quantifying Fluorescent Reporters, processes in intact animals. Here, we discuss the basic microscope components required to observe, image, and measure fluorescent proteins in live animals for students and researchers who work with . but have limited experience with fluorescence imaging and analysis.作者: 尾巴 時(shí)間: 2025-3-24 13:54 作者: 不滿分子 時(shí)間: 2025-3-24 15:59 作者: 樹(shù)木中 時(shí)間: 2025-3-24 22:56 作者: SLAY 時(shí)間: 2025-3-24 23:20
The State of the Art in Microformats processes in intact animals. Here, we discuss the basic microscope components required to observe, image, and measure fluorescent proteins in live animals for students and researchers who work with . but have limited experience with fluorescence imaging and analysis.作者: Hemoptysis 時(shí)間: 2025-3-25 04:24 作者: 不能強(qiáng)迫我 時(shí)間: 2025-3-25 09:15 作者: 身體萌芽 時(shí)間: 2025-3-25 15:26 作者: Foreshadow 時(shí)間: 2025-3-25 15:53 作者: 圓柱 時(shí)間: 2025-3-25 21:46
An Imaging System for Monitoring , Behavior and Aging,Many experiments in . neurobiology rely on imaging its behavior. Here we describe procedures for building a flexible and inexpensive imaging system using standard optical and mechanical components.作者: 中世紀(jì) 時(shí)間: 2025-3-26 03:59
Location Microformats: GEO and ADRon setting up the laboratory for . work, and on putting into place effective strategies for running a productive and inclusive research group. Also discussed are strategies for managing the group, standard practices in the . field, lists of resources, and several sample handouts for new research gro作者: Indigence 時(shí)間: 2025-3-26 06:06
Location Microformats: GEO and ADRts genome and biology, its short-generation time and the ease of working with it in the lab. However, it can be daunting to begin a short-term project or a teaching lab with an organism with which one has little or no experience. Therefore, in this article, we will discuss the minimal equipment, rea作者: 防御 時(shí)間: 2025-3-26 11:11 作者: Generic-Drug 時(shí)間: 2025-3-26 13:46 作者: Hyperopia 時(shí)間: 2025-3-26 19:23 作者: tattle 時(shí)間: 2025-3-26 22:45 作者: 針葉樹(shù) 時(shí)間: 2025-3-27 05:07 作者: Ondines-curse 時(shí)間: 2025-3-27 06:14 作者: BIDE 時(shí)間: 2025-3-27 12:07 作者: aggrieve 時(shí)間: 2025-3-27 17:18 作者: CHANT 時(shí)間: 2025-3-27 21:47
,Gas–Liquid Flow Pressure Drop,ptimized methods to extract and analyze lipids from the nematode . based on standard methods. Here we describe a method to extract total lipids from . larvae, adults, or embryos. We describe a thin-layer chromatography method to separate major lipid classes and a gas chromatography method to analyze作者: myocardium 時(shí)間: 2025-3-28 01:31 作者: Mhc-Molecule 時(shí)間: 2025-3-28 05:37 作者: 尾隨 時(shí)間: 2025-3-28 07:16
,Gas–Liquid Flow Pressure Drop,nique was pioneered in . where it led to exciting discoveries in the fields of development and neurobiology. All neurons studied so far in . can regenerate and regrow axons and dendrites after injury, allowing studies of the molecular and cellular basis of neuroregeneration. In this chapter, we desc作者: CLEAR 時(shí)間: 2025-3-28 11:53
,Gas–Liquid Flow Pressure Drop,died offer only a narrow glimpse into the richness of natural behaviors this remarkable animal evolved over 500 million years of evolution. For example, burrowing behavior naturally occurs in the wild, but it remains understudied. Our group studies burrowing in an attempt to expand our understanding作者: 迅速成長(zhǎng) 時(shí)間: 2025-3-28 17:55
Starting a , Research Laboratory: Practical Advice,on setting up the laboratory for . work, and on putting into place effective strategies for running a productive and inclusive research group. Also discussed are strategies for managing the group, standard practices in the . field, lists of resources, and several sample handouts for new research gro作者: BOOST 時(shí)間: 2025-3-28 19:08 作者: 堅(jiān)毅 時(shí)間: 2025-3-28 23:33 作者: AUGER 時(shí)間: 2025-3-29 03:35 作者: 可以任性 時(shí)間: 2025-3-29 10:20 作者: Injunction 時(shí)間: 2025-3-29 14:22
Optogenetic Perturbation of Individual , Pharyngeal Neurons While Monitoring Feeding Behavior,ctrophysiological manipulation is not possible. Here we describe a method for optogenetically manipulating single pharyngeal neurons in intact . while monitoring pharyngeal behavior. This approach provides bidirectional and dynamic control of pharyngeal neural activity while quantitatively assessing作者: 花爭(zhēng)吵 時(shí)間: 2025-3-29 19:02
Antibody Staining for Nematodes with Heat-induced Antigen Retrieval (HIAR),ecular genetic tools and transgenics. Because of the highly idiosyncratic nature of successful immunostaining procedures, innovations can still be found for this long-established technique. Heat-induced antigen retrieval (HIAR) is well known from other systems, but seems not to have been applied to 作者: 線 時(shí)間: 2025-3-29 22:55 作者: Inflated 時(shí)間: 2025-3-30 00:26 作者: OVERT 時(shí)間: 2025-3-30 08:06 作者: Perceive 時(shí)間: 2025-3-30 10:49 作者: 阻撓 時(shí)間: 2025-3-30 14:55
Isolating , from the Natural Habitat, Here we provide a simple protocol on how to sample and rapidly identify . wild isolates. We outline how to find suitable habitats and organic substrates, followed by describing isolation and identification of . live cultures based on easily recognizable morphological characteristics, molecular barc作者: 相同 時(shí)間: 2025-3-30 18:53
Microfluidic Devices for Behavioral Analysis, Microscopy, and Neuronal Imaging in ,mals during imaging, quantifying behavior, and automating screens. However, challenges to their widespread adoption in the field include increased complexity over conventional methods, operational problems (such as clogging, leaks, and bubbles), difficulty in obtaining or fabricating devices, and th作者: Budget 時(shí)間: 2025-3-31 00:30
Neuronal Microsurgery with an Yb-Doped Fiber Femtosecond Laser,nique was pioneered in . where it led to exciting discoveries in the fields of development and neurobiology. All neurons studied so far in . can regenerate and regrow axons and dendrites after injury, allowing studies of the molecular and cellular basis of neuroregeneration. In this chapter, we desc作者: Slit-Lamp 時(shí)間: 2025-3-31 03:50 作者: Pillory 時(shí)間: 2025-3-31 05:22
Location Microformats: GEO and ADR introductory or advanced neuroscience undergraduate courses where students learn to understand the relationship between genetic mutations and behavior through using chemotaxis assays. In addition, instructions for students for the lab module are provided in Subheading “Example of Teaching Lab Module for Students”.作者: 凝乳 時(shí)間: 2025-3-31 13:00 作者: dapper 時(shí)間: 2025-3-31 16:20 作者: Water-Brash 時(shí)間: 2025-3-31 20:35 作者: 場(chǎng)所 時(shí)間: 2025-4-1 00:51
Cryopreservation of , and Other Nematodes with Dimethyl Sulfoxide and Trehalose,t survive a typical freeze–thaw cycle. Here I describe an alternative method based on the popular combination of DMSO and trehalose as a cryoprotectant. This method allows the survival of large numbers of worms and effectively protects most developmental stages including adults.作者: 凈禮 時(shí)間: 2025-4-1 03:06 作者: Trypsin 時(shí)間: 2025-4-1 06:51 作者: 一窩小鳥(niǎo) 時(shí)間: 2025-4-1 13:06 作者: Lumbar-Spine 時(shí)間: 2025-4-1 14:44 作者: tolerance 時(shí)間: 2025-4-1 20:42 作者: adhesive 時(shí)間: 2025-4-1 23:06 作者: 過(guò)剩 時(shí)間: 2025-4-2 05:03
1064-3745 expertsThis detailed new edition provides a guide for implementing a selection of novel techniques utilizing the pioneering organism, .Caenorhabditis elegans., a vital discovery engine for the rules of life, from molecules and genes, through cellular physiology, metabolism and aging, neurobiology a
