標(biāo)題: Titlebook: Bone Morphogenetic Proteins; Methods and Protocol Melissa B. Rogers Book 2019 Springer Science+Business Media, LLC, part of Springer Nature [打印本頁(yè)] 作者: TEMPO 時(shí)間: 2025-3-21 16:26
書目名稱Bone Morphogenetic Proteins影響因子(影響力)
作者: Basal-Ganglia 時(shí)間: 2025-3-21 22:15 作者: prostatitis 時(shí)間: 2025-3-22 03:30
Cell-Based Gene Therapy System for Delivering BMPs,with accurate posttranslational processing. The use of transduced cells eliminates viral toxicity, allows for targeted expression of the secreted factor at a specific site, and ensures that the therapy will be turned off when the cells are cleared by the organism. Here we describe the delivery syste作者: 調(diào)色板 時(shí)間: 2025-3-22 08:19
Generation of Endogenous BMP Transcriptional Reporter Cells Through CRISPR/Cas9 Genome Editing,tificial cDNA overexpression vectors containing either a promoter fragment or specific nucleotide sequence repeats upstream of . luciferase or fluorescent reporters, such as green fluorescence protein (GFP) cDNA. These systems suffer mainly from the lack of chromatin context. Here, we describe the r作者: 大罵 時(shí)間: 2025-3-22 11:59 作者: MIR 時(shí)間: 2025-3-22 16:38
Mutagenesis and Imaging Studies of BMP Signaling Mechanisms in ,,onents and the power of genetic and cell biological approaches applied toward understanding how the pathway signals. In Subheading ., we detail approaches to study the BMP branch of the TGFβ pathway in ..作者: Neutropenia 時(shí)間: 2025-3-22 19:38
Gene Regulation of BMP Ligands in ,ols available. . has three BMP ligands, . (BMP2/4), ., and . (BMP5/6/7/8). Of these genes, the transcriptional regulation of . has been studied, and some of the enhancers directing its spatially specific gene expression have been described. These analyses have used many of the standard tools of mole作者: surrogate 時(shí)間: 2025-3-22 21:37 作者: 用樹皮 時(shí)間: 2025-3-23 01:34
Proteolytic Activation of Bmps: Analysis of Cleavage in , Oocytes and Embryos,that can modulate growth factor activity. Here we provide three protocols that can be used to examine the process of proteolytic activation of Bmps. The first protocol describes how to generate radiolabeled Bmp precursor proteins in . oocytes and then analyze the time course of precursor cleavage by作者: 柏樹 時(shí)間: 2025-3-23 09:07 作者: foppish 時(shí)間: 2025-3-23 11:09 作者: 天賦 時(shí)間: 2025-3-23 14:20
Generation and Identification of Genetically Modified Mice for BMP Receptors,een established to study BMP function in development and disease. Mice with altered BMP receptor genes (including global knockout, conditional knockout, and conditional constitutively active transgenic mouse lines) have been particularly informative. In this chapter, we describe how the genetically 作者: interior 時(shí)間: 2025-3-23 19:20
Phenotypic Analyses of Genetically Modified Mice for BMP Receptors, particularly informative for clarifying the role of BMP signaling. In this chapter, we introduce several selected protocols for in vivo functional characterization of BMP receptors in genetically modified mice, including immunohistochemistry of BMP downstream signaling (P-Smad1/5/9 or others), hist作者: cylinder 時(shí)間: 2025-3-24 00:04
Immunofluorescent Visualization of BMP Signaling Activation on Paraffin-Embedded Tissue Sections,bodies to bind to specific antigens. Cellular signaling can be visualized using antibodies raised against phosphorylated proteins. Phosphorylated Smad1, Smad5, and Smad9 are the activated signaling molecules of the BMP pathway that transfer BMP signals from the cell surface to the nucleus. Here we d作者: capillaries 時(shí)間: 2025-3-24 05:48
Spatial and Quantitative Detection of BMP Activity in Mouse Embryonic Limb Buds, BMP signaling at various stages of limb development causes defects ranging from complete limb agenesis to oligodactyly, polydactyly, webbing, and chondrodysplasia. To probe the state of BMP signaling in early limb buds, we designed two sets of primers to measure both spatially and quantitatively th作者: 越自我 時(shí)間: 2025-3-24 08:05
Pharmacologic Strategies for Assaying BMP Signaling Function,y diverse receptors and ligands whose combinatorial specificity encodes autocrine, paracrine, and endocrine signals essential for regulating tissue growth, differentiation, and survival during embryonic patterning and postnatal tissue remodeling. Aberrant signaling of these receptors and ligands is 作者: Cougar 時(shí)間: 2025-3-24 14:38
Bone Morphogenetic Proteins (BMPs) and Bone Regeneration,e studies and provides details on the rat femur defect model. This animal model is frequently utilized to measure the efficacy of osteogenic factors like BMP-2. Detailed information about delivery methods, dose range, and dose duration used in BMP-2-related studies are provided.作者: 吹牛大王 時(shí)間: 2025-3-24 15:46 作者: exophthalmos 時(shí)間: 2025-3-24 19:08 作者: reptile 時(shí)間: 2025-3-24 23:49
1064-3745 tion advice from the experts.This volume provides methods to study bone morphogenetic proteins (BMPs) and BMP modulators in cell culture, in both invertebrate and vertebrate animal models, and for therapeutic applications. Chapters guide the reader in the use of primary and immortalized cell lines, 作者: Corporeal 時(shí)間: 2025-3-25 05:22 作者: 反話 時(shí)間: 2025-3-25 07:50 作者: Lobotomy 時(shí)間: 2025-3-25 11:50 作者: ROOF 時(shí)間: 2025-3-25 17:09
Phenotypic Analyses of Genetically Modified Mice for BMP Receptors,aracterization of BMP receptors in genetically modified mice, including immunohistochemistry of BMP downstream signaling (P-Smad1/5/9 or others), histological analysis, whole-mount skeletal staining for cartilage and bone tissues, and whole-mount cartilage staining.作者: deficiency 時(shí)間: 2025-3-25 20:39 作者: Pudendal-Nerve 時(shí)間: 2025-3-26 00:50
Double-Humanized Mouse Model to Study Bone Morphogenetic Protein (BMP) Signaling in Tumor Xenograftment of BMP receptor inhibitors has allowed the study of tumor xenografts in mice. We describe a double-humanized mouse model with adoptively transferred human immune and human tumor cells that can be used to assess the effects of BMP inhibitors on these human cells in vivo.作者: 制度 時(shí)間: 2025-3-26 05:20
Book 2019al models, and for therapeutic applications. Chapters guide the reader in the use of primary and immortalized cell lines, methodologies that exploit transcription and receptor mechanisms, newly developed animal models using nematodes, flies, amphioxus, frogs, zebrafish, and genetically engineered mi作者: Defiance 時(shí)間: 2025-3-26 10:47 作者: Tracheotomy 時(shí)間: 2025-3-26 15:27 作者: Corporeal 時(shí)間: 2025-3-26 18:27 作者: antiquated 時(shí)間: 2025-3-27 00:39 作者: 蟄伏 時(shí)間: 2025-3-27 04:03
Book 2019agents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls...??Authoritative and cutting-edge, .Bone Morphogenetic Proteins: Methods and Protocols. aims to ensure successful results in the further study of this vital field..作者: 表主動(dòng) 時(shí)間: 2025-3-27 08:31 作者: 多骨 時(shí)間: 2025-3-27 12:50 作者: CUB 時(shí)間: 2025-3-27 17:37
Anne Jardin,Karine Broto,Timothée Perdrizet whole-mount mRNA in situ hybridization, while the second set is used for SYBR. Green-based quantitative PCR on limb bud cDNA. Here we describe step-by-step protocols for both methods around this specific set of genes.作者: 機(jī)密 時(shí)間: 2025-3-27 21:36 作者: 馬賽克 時(shí)間: 2025-3-28 01:43 作者: micturition 時(shí)間: 2025-3-28 03:28
Spatial and Quantitative Detection of BMP Activity in Mouse Embryonic Limb Buds, whole-mount mRNA in situ hybridization, while the second set is used for SYBR. Green-based quantitative PCR on limb bud cDNA. Here we describe step-by-step protocols for both methods around this specific set of genes.作者: ACTIN 時(shí)間: 2025-3-28 08:01
Cell-Based Gene Therapy System for Delivering BMPs,m which utilizes cells transduced with a non-replicating adenovirus containing bone morphogenetic protein 2 (BMP-2) in the E1 region of the cassette. With this method of delivery, small amounts of the protein can incite de novo bone formation.作者: 指耕作 時(shí)間: 2025-3-28 12:39 作者: ASTER 時(shí)間: 2025-3-28 16:35
Generation and Identification of Genetically Modified Mice for BMP Receptors,modified mice were generated and introduce genotyping methods. These methods include regular PCR and genomic real-time PCR using specific primers based on different constructs in different mice strains.作者: 舊石器時(shí)代 時(shí)間: 2025-3-28 21:42 作者: Detonate 時(shí)間: 2025-3-29 01:04
https://doi.org/10.1007/978-3-663-01758-5m which utilizes cells transduced with a non-replicating adenovirus containing bone morphogenetic protein 2 (BMP-2) in the E1 region of the cassette. With this method of delivery, small amounts of the protein can incite de novo bone formation.作者: 繼而發(fā)生 時(shí)間: 2025-3-29 03:38
Anne Jardin,Karine Broto,Timothée Perdrizeten result in enhanced HO, which can exacerbate rather than improve diagnostic outcomes. As a result of these difficulties, a variety of animal models are needed to study human FOP. Here we describe the methods for creating and characterizing zebrafish conditionally expressing Acvr1l., the first adult zebrafish model for FOP.作者: Compass 時(shí)間: 2025-3-29 07:14
J.-P. Burg,A. Dolati,D. Bernoulli,J. Smitmodified mice were generated and introduce genotyping methods. These methods include regular PCR and genomic real-time PCR using specific primers based on different constructs in different mice strains.作者: 提名 時(shí)間: 2025-3-29 11:43
https://doi.org/10.1007/978-3-319-73942-7n are being made through the use of genetically engineered mouse models that recapitulate the human disease. We describe methods for inducing heterotopic ossification in a mouse model that conditionally expresses the .. allele.作者: MAIM 時(shí)間: 2025-3-29 16:36
1064-3745 ls...??Authoritative and cutting-edge, .Bone Morphogenetic Proteins: Methods and Protocols. aims to ensure successful results in the further study of this vital field..978-1-4939-8904-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 悲觀 時(shí)間: 2025-3-29 19:48 作者: SOBER 時(shí)間: 2025-3-30 03:18
https://doi.org/10.1007/978-3-319-44726-1yeast FLP/. recombination system facilitates experiments to interrogate loss- or gain-of-function for transcription factor activity on known enhancers. This chapter will outline the necessary steps to create the tools needed and conduct somatic cell recombination experiments to interrogate the funct作者: 模范 時(shí)間: 2025-3-30 07:58 作者: Frequency 時(shí)間: 2025-3-30 08:40
,High-Throughput, Biosensor-Based Approach to Examine Bone Morphogenetic Protein (BMP)–Receptor Inte this concentration range, equilibrium dissociation constants for high-affinity and medium-affinity binders can be estimated with good accuracy and with great precision from the single injection binding curves.作者: 使腐爛 時(shí)間: 2025-3-30 14:15
Gene Regulation of BMP Ligands in ,yeast FLP/. recombination system facilitates experiments to interrogate loss- or gain-of-function for transcription factor activity on known enhancers. This chapter will outline the necessary steps to create the tools needed and conduct somatic cell recombination experiments to interrogate the funct作者: encomiast 時(shí)間: 2025-3-30 17:28 作者: Defense 時(shí)間: 2025-3-31 00:17
Common Cell Lines Used to Study Bone Morphogenetic Proteins (BMPs),types used to measure the efficacy of osteogenic factors like BMP-2. Detailed information regarding media and culture conditions are provided. Parameters relevant to experimental reproducibility and cell line authentication are discussed.作者: BUDGE 時(shí)間: 2025-3-31 03:28 作者: Angioplasty 時(shí)間: 2025-3-31 06:36
Mutagenesis and Imaging Studies of BMP Signaling Mechanisms in ,,onents and the power of genetic and cell biological approaches applied toward understanding how the pathway signals. In Subheading ., we detail approaches to study the BMP branch of the TGFβ pathway in ..作者: nuclear-tests 時(shí)間: 2025-3-31 13:05
Bone Morphogenetic Proteins (BMPs) and Bone Regeneration,e studies and provides details on the rat femur defect model. This animal model is frequently utilized to measure the efficacy of osteogenic factors like BMP-2. Detailed information about delivery methods, dose range, and dose duration used in BMP-2-related studies are provided.作者: Dungeon 時(shí)間: 2025-3-31 16:48 作者: 分貝 時(shí)間: 2025-3-31 18:54 作者: 叫喊 時(shí)間: 2025-3-31 23:56 作者: 男生戴手銬 時(shí)間: 2025-4-1 01:51
Albrecht Rabenau,Ulrich Wannagattificial cDNA overexpression vectors containing either a promoter fragment or specific nucleotide sequence repeats upstream of . luciferase or fluorescent reporters, such as green fluorescence protein (GFP) cDNA. These systems suffer mainly from the lack of chromatin context. Here, we describe the r作者: 金絲雀 時(shí)間: 2025-4-1 08:21
Chuanlong Mou,Xiuping Wang,Wei Liang form is vital for understanding the physiological activities of a particular BMP. Here, we describe a surface plasmon resonance (SPR)-based, high-throughput approach that allows fast identification and evaluation of BMP–receptor complexes. Briefly, the extracellular, BMP-binding domains of receptor作者: 支架 時(shí)間: 2025-4-1 12:39
Chuanlong Mou,Xiuping Wang,Wei Liangonents and the power of genetic and cell biological approaches applied toward understanding how the pathway signals. In Subheading ., we detail approaches to study the BMP branch of the TGFβ pathway in ..作者: 原始 時(shí)間: 2025-4-1 16:20
