標題: Titlebook: Basic Cell Culture Protocols; Cheryl D. Helgason,Cindy L. Miller Book 20053rd edition Humana Press 2005 DNA.Macrophages.cell.cell culture. [打印本頁] 作者: Hazardous 時間: 2025-3-21 19:37
書目名稱Basic Cell Culture Protocols影響因子(影響力)
書目名稱Basic Cell Culture Protocols影響因子(影響力)學科排名
書目名稱Basic Cell Culture Protocols網(wǎng)絡公開度
書目名稱Basic Cell Culture Protocols網(wǎng)絡公開度學科排名
書目名稱Basic Cell Culture Protocols被引頻次
書目名稱Basic Cell Culture Protocols被引頻次學科排名
書目名稱Basic Cell Culture Protocols年度引用
書目名稱Basic Cell Culture Protocols年度引用學科排名
書目名稱Basic Cell Culture Protocols讀者反饋
書目名稱Basic Cell Culture Protocols讀者反饋學科排名
作者: 中子 時間: 2025-3-21 21:02 作者: 有權威 時間: 2025-3-22 01:08
Ergebnisse des quantitativen Studienteils,eadily accessible at the level of the intact organism. Successful maintenance of cells in culture, whether primary or immortalized, requires knowledge and practice of a few essential techniques. The purpose of this chapter is to explain the basic principles of cell culture using the maintenance of a作者: enflame 時間: 2025-3-22 05:43 作者: faction 時間: 2025-3-22 09:57 作者: Bother 時間: 2025-3-22 14:54
https://doi.org/10.1007/978-3-476-05768-6es. In this regard, it is crucial that the cells faithfully correspond to the purported objects of study. A number of recent publications have shown an unacceptable level of cell lines to be false, in part as a result of the nonavailability of a simple and easy DNA profiling technique. We have valid作者: 莎草 時間: 2025-3-22 17:55 作者: LATE 時間: 2025-3-23 00:10
Maiken Umbach,Claus-Christian W. Szejnmannprogenitors that can be detected in vitro using colony-forming cell (CFC) assays. Hematopoietic progenitor cells, when cultured in a semisolid methylcellulose-based medium that is supplemented with suitable growth factors, proliferate and differentiate to produce clonal clusters (colonies) of maturi作者: 神經(jīng) 時間: 2025-3-23 05:00
https://doi.org/10.1007/978-0-230-39111-6ly be harvested from mice and purified by adherence to tissue culture plastic. The injection of Bio-Gel polyacrylamide beads or thioglycollate broth into the peritoneal cavity produces an inflammatory response allowing the purification of large numbers of elicited macrophages. The production of an a作者: 擦掉 時間: 2025-3-23 06:38
https://doi.org/10.1007/978-0-230-39111-6rations by Ficoll density gradient centrifugation, followed by adhesion-mediated purification on tissue culture or gelatin-coated plastic. The monocytes differentiate into macrophages in vitro by culturing in medium containing autologous human fibrin-depleted plasma. Alveolar macrophages can be puri作者: 富饒 時間: 2025-3-23 11:01
Maiken Umbach,Claus-Christian W. Szejnmannt in FTOC well represents fetal thymocyte development in vivo. Here, we describe the basic method for FTOC as well as several related techniques, including the reconstitution of thymus lobes with T-lymphoid progenitor cells, high-oxygen submersion culture, time-lapse visualization of thymic emigrati作者: 音樂會 時間: 2025-3-23 15:36 作者: 躲債 時間: 2025-3-23 21:26
https://doi.org/10.1007/978-3-658-22253-6s of human blood development. This chapter describes two methods to promote hematopoietic differentiation of human ES cells: stromal cell coculture and embryoid body formation. Better understanding of basic human hematopoiesis through the study of human ES cells will likely have future therapeutic b作者: AVOW 時間: 2025-3-23 23:43 作者: 惡名聲 時間: 2025-3-24 04:25
Der deutsche Hans kehrt heim ins Glückonment cells, colony-forming-unit fibroblasts (CFU-F), and mesenchymal cells. These cells were originally thought to provide an appropriate matrix for hematopoietic cell development, but recent examination of these cell populations suggests a much broader spectrum of activity, including the generati作者: 粗鄙的人 時間: 2025-3-24 08:32
https://doi.org/10.1007/978-3-658-04740-5d maintain protection from adverse environmental exposure. The keratinocyte is the cell primarily responsible for this structure. Isolation and in vitro cultivation of this cell type is widely used in dermatological and other investigations as opposed to using whole animals. However, this cell is ve作者: ELATE 時間: 2025-3-24 11:02 作者: inveigh 時間: 2025-3-24 17:38
https://doi.org/10.1007/978-3-658-04740-5 are purified from the rabbit kidney by the method of Brendel and Meezan. To summarize, each kidney is perfused with iron oxide, which becomes associated with glomeruli. The renal cortex is sliced and homogenized to liberate nephron segments. Renal proximal tubules and glomeruli are purified by siev作者: 誘拐 時間: 2025-3-24 21:26 作者: 租約 時間: 2025-3-25 02:19 作者: Arthr- 時間: 2025-3-25 06:52 作者: 反對 時間: 2025-3-25 11:01
Hematopoietic Development of Human Embryonic Stem Cells in Culture,s of human blood development. This chapter describes two methods to promote hematopoietic differentiation of human ES cells: stromal cell coculture and embryoid body formation. Better understanding of basic human hematopoiesis through the study of human ES cells will likely have future therapeutic benefits.作者: Ferritin 時間: 2025-3-25 14:31
Cheryl D. Helgason,Cindy L. MillerIncludes supplementary material: 作者: 加花粗鄙人 時間: 2025-3-25 17:54
Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/180966.jpg作者: 煉油廠 時間: 2025-3-25 22:57
https://doi.org/10.1385/1592598382DNA; Macrophages; cell; cell culture; cell lines; development; enzyme; human cell lines; neural stem cells; s作者: 狂熱文化 時間: 2025-3-26 02:17 作者: 向下五度才偏 時間: 2025-3-26 08:01
Maiken Umbach,Claus-Christian W. Szejnmannuding the reconstitution of thymus lobes with T-lymphoid progenitor cells, high-oxygen submersion culture, time-lapse visualization of thymic emigration, reaggregation culture, and retrovirus-mediated gene transfer to developing thymocytes in FTOC.作者: Bumble 時間: 2025-3-26 08:32 作者: Coma704 時間: 2025-3-26 14:48
Culture of Primary Adherent Cells and a Continuously Growing Nonadherent Cell Line, nonadherent cell line, the P815 mouse mastocytoma cell line, and the isolation and culture of adherent primary mouse embryonic fibroblasts (MEFs) as examples. Procedures for thawing, culture, determination of cell numbers and viability, and cryopreservation are described.作者: 欺騙世家 時間: 2025-3-26 19:10 作者: babble 時間: 2025-3-26 21:42 作者: 頂點 時間: 2025-3-27 03:48
Ergebnisse des quantitativen Studienteils, nonadherent cell line, the P815 mouse mastocytoma cell line, and the isolation and culture of adherent primary mouse embryonic fibroblasts (MEFs) as examples. Procedures for thawing, culture, determination of cell numbers and viability, and cryopreservation are described.作者: 改正 時間: 2025-3-27 05:33 作者: 天賦 時間: 2025-3-27 11:18 作者: Armory 時間: 2025-3-27 14:40
Book 20053rd editionisolation and growth of major primary cell types, such as kidney proximal tubule cells, hepatocytes, keratinocytes, and cardiomyocytes. The authors offer readily reproducible new methods for the differentiation of embryonic stem (ES) cells into various hematopoietic cell types, for fetal thymic orga作者: 反復拉緊 時間: 2025-3-27 19:30 作者: 取消 時間: 2025-3-27 23:14
https://doi.org/10.1007/978-3-476-05768-6t mycoplasma strains, and to cure heavily contaminated and damaged cells. To date, no consistent and permanent alterations that affect the eukaryotic cells during or after the treatment have been detected.作者: 品牌 時間: 2025-3-28 04:23 作者: 起波瀾 時間: 2025-3-28 07:55 作者: 準則 時間: 2025-3-28 12:27 作者: synovial-joint 時間: 2025-3-28 17:03
Der deutsche Hans kehrt heim ins Glück and to enhance the engraftment of hematopoietic cells. This chapter describes methods for the human CFU-F assay, culture and expansion of mesenchymal cells, as well as their differentiation to adipocytes. In addition, this chapter describes the mouse CFU-F assay.作者: colony 時間: 2025-3-28 21:48
https://doi.org/10.1007/978-3-658-04740-5 chapter describes the methodology required to isolate, purify, and cultivate keratinocytes to produce both monolayer and stratified cultures. The methodologies for producing cultures of keratinocytes obtained from rat skin and from human skin are described.作者: glisten 時間: 2025-3-29 01:19
https://doi.org/10.1007/978-3-658-04740-5es are plated in hormonally defined serum-free medium supplemented with 5 μg/mL bovine insulin, 5 μg/mL human transferrin, and 5 × 10.. hydrocortisone. After 5–6 d of incubation, confluent monolayers are obtained that possess multicellular domes, indicative of their capacity for transepithelial solute transport.作者: Gourmet 時間: 2025-3-29 04:53 作者: Adjourn 時間: 2025-3-29 09:44 作者: subacute 時間: 2025-3-29 12:16
Eradication of Mycoplasma Contaminations,t mycoplasma strains, and to cure heavily contaminated and damaged cells. To date, no consistent and permanent alterations that affect the eukaryotic cells during or after the treatment have been detected.作者: tattle 時間: 2025-3-29 15:45
Human and Mouse Hematopoietic Colony-Forming Cell Assays,genitors and committed progenitors of the erythroid, monocyte, and granulocyte lineages in samples from human peripheral blood, bone marrow, and cord blood as well as mouse fetal liver and bone marrow are described.作者: RAGE 時間: 2025-3-29 21:42
Isolation and Culture of Murine Macrophages, isolated following enzymatic separation of cells from bone marrow plugs and enrichment on 30% fetal calf serum containing medium or Ficoll-Hypaque gradients. Bone marrow-derived macrophages can be produced by differentiating nonadherent macrophage precursors with medium containing macrophage colony-stimulating factor.作者: resilience 時間: 2025-3-30 02:19 作者: 得意人 時間: 2025-3-30 05:03 作者: Paraplegia 時間: 2025-3-30 11:31
Isolation, Purification, and Cultivation of Murine and Human Keratinocytes, chapter describes the methodology required to isolate, purify, and cultivate keratinocytes to produce both monolayer and stratified cultures. The methodologies for producing cultures of keratinocytes obtained from rat skin and from human skin are described.作者: 星球的光亮度 時間: 2025-3-30 15:34 作者: Fraudulent 時間: 2025-3-30 20:37 作者: 抒情短詩 時間: 2025-3-30 23:56 作者: 贊美者 時間: 2025-3-31 03:30 作者: 玩笑 時間: 2025-3-31 07:36
Book 20053rd editionsolation of side-population cells, and scalable production of ES-derived cells) and detail quality control methods for cell lines (detection and elimination of mycoplasma, DNA fingerprinting, and cytogenetic analysis).作者: corpus-callosum 時間: 2025-3-31 09:12 作者: prostatitis 時間: 2025-3-31 16:42
Detection of Mycoplasma Contaminations,oplasma contamination is an important part of mycoplasma control and should be an established method in every cell culture laboratory. New cell lines as well as cell lines in continuous culture must be tested in regular intervals. The polymerase chain reaction (PCR) methodology offers a fast and sen作者: giggle 時間: 2025-3-31 18:52
Eradication of Mycoplasma Contaminations,active substances used in human medicine. The elimination of mycoplasma contaminations in cell cultures has become a practical alternative to discarding and re-establishing important or irreplaceable cell lines. Different quinolones, tetracyclins, and macrolides shown to have strong antimycoplasma p作者: Analogy 時間: 2025-3-31 23:41
Authentication of Scientific Human Cell Lines,es. In this regard, it is crucial that the cells faithfully correspond to the purported objects of study. A number of recent publications have shown an unacceptable level of cell lines to be false, in part as a result of the nonavailability of a simple and easy DNA profiling technique. We have valid作者: AUGUR 時間: 2025-4-1 03:55 作者: Graduated 時間: 2025-4-1 09:22
Human and Mouse Hematopoietic Colony-Forming Cell Assays,progenitors that can be detected in vitro using colony-forming cell (CFC) assays. Hematopoietic progenitor cells, when cultured in a semisolid methylcellulose-based medium that is supplemented with suitable growth factors, proliferate and differentiate to produce clonal clusters (colonies) of maturi作者: extemporaneous 時間: 2025-4-1 10:46 作者: GLIB 時間: 2025-4-1 15:28 作者: 陳腐思想 時間: 2025-4-1 22:06 作者: 口味 時間: 2025-4-2 01:51 作者: 生命 時間: 2025-4-2 04:34
Hematopoietic Development of Human Embryonic Stem Cells in Culture,s of human blood development. This chapter describes two methods to promote hematopoietic differentiation of human ES cells: stromal cell coculture and embryoid body formation. Better understanding of basic human hematopoiesis through the study of human ES cells will likely have future therapeutic b作者: 思考才皺眉 時間: 2025-4-2 09:32
Generation of Murine Stromal Cell Lines,rom subdissected AGM regions in “explant-” or “single-cell suspension”-type cultures from embryos transgenic for ., a temperature-sensitive mutant of the SV40 T antigen gene. The number of cells in such cultures initially expand, but in most cases, this expansion phase is followed by a stable or eve作者: 不能根除 時間: 2025-4-2 13:00
Culture of Human and Mouse Mesenchymal Cells,onment cells, colony-forming-unit fibroblasts (CFU-F), and mesenchymal cells. These cells were originally thought to provide an appropriate matrix for hematopoietic cell development, but recent examination of these cell populations suggests a much broader spectrum of activity, including the generati
