作者: Chemotherapy 時(shí)間: 2025-3-22 00:18
https://doi.org/10.1007/978-1-0716-1621-5Microfluidics; flow cytometry; Metabolomics; aminoglycosides; population dynamics作者: Inculcate 時(shí)間: 2025-3-22 01:22 作者: xanthelasma 時(shí)間: 2025-3-22 05:45 作者: 和平 時(shí)間: 2025-3-22 10:45 作者: ENNUI 時(shí)間: 2025-3-22 13:42
https://doi.org/10.1007/978-3-531-92015-3alled persister phenotype, which makes it difficult to study this health-threatening phenotype. We recently found that certain abrupt nutrient shifts generate . populations that consist almost entirely of antibiotic-tolerant cells. These nearly homogeneous persister cell populations enable assessmen作者: eulogize 時(shí)間: 2025-3-22 17:31 作者: 駭人 時(shí)間: 2025-3-22 23:43
Rudolf Tippelt,Bernhard Schmidtlly, the abundance of persister cells is very low (about 1 in 10. cells) in actively growing bacterial populations. Therefore, in order to study bacterial persistence mechanisms for therapeutics development, persister cells need to be enriched from a larger culture. Here, we describe three enrichmen作者: 辭職 時(shí)間: 2025-3-23 01:39 作者: conscience 時(shí)間: 2025-3-23 09:11
Henning P?tzold,Albert Schmelzerr the antibiotic treatment. Therefore, time-lapse microscopy is the gold-standard method to investigate this phenomenon. Here, we describe an exhaustive procedure for acquiring single-cell data which is particularly suitable for persister cell analysis but could be applied to any other fields of res作者: Apoptosis 時(shí)間: 2025-3-23 12:57
https://doi.org/10.1007/978-3-662-54368-9spatial and temporal variation in nutrient availability lead to increased survival of bacteria to antibiotics. The first setup is designed to mimic the growth dynamics of bacteria in spatially structured populations (e.g., biofilms) and can be used to study how spatial gradients in nutrient availabi作者: Liability 時(shí)間: 2025-3-23 16:45 作者: 得體 時(shí)間: 2025-3-23 18:14 作者: Counteract 時(shí)間: 2025-3-24 00:25 作者: Constant 時(shí)間: 2025-3-24 03:20 作者: 閑蕩 時(shí)間: 2025-3-24 06:48
Biographie – Genealogie – Generationingle-cell techniques and newly obtained insights in the persister phenotype, studying persister awakening has been unlocked and it has gained much interest in the scientific community. However, a framework on how this research should be conducted is currently lacking. Therefore, we here present a m作者: 輕率看法 時(shí)間: 2025-3-24 10:48 作者: 原來(lái) 時(shí)間: 2025-3-24 16:52 作者: 脫水 時(shí)間: 2025-3-24 21:46
Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/180312.jpg作者: 慢慢流出 時(shí)間: 2025-3-25 02:40 作者: 大約冬季 時(shí)間: 2025-3-25 03:53
Maria Montessoris p?dagogischer Reformimpulsstence. We describe a method of single-cell imaging using glass bottom dishes and a nutrient agarose pad that allows for long-term single-cell microscopy observation in a stable environment. We apply this method to characterize the lag phase and persistence of individual . cells.作者: 天賦 時(shí)間: 2025-3-25 11:20 作者: 莊嚴(yán) 時(shí)間: 2025-3-25 12:54
Helmut Niegemann,Armin Weinbergerin persister cells, which are isolated by treatment with beta-lactam antibiotics. Furthermore, persister proteomes during the postantibiotic recovery phase are analyzed by label-free quantification. The presented methods are valuable tools to shed light on persister physiology.作者: Emasculate 時(shí)間: 2025-3-25 17:06
Biographie – Genealogie – Generationterest in the scientific community. However, a framework on how this research should be conducted is currently lacking. Therefore, we here present a method to detect and validate genes important for persister awakening.作者: 獨(dú)白 時(shí)間: 2025-3-25 23:47
Observing Bacterial Persistence at Single-Cell Resolutionstence. We describe a method of single-cell imaging using glass bottom dishes and a nutrient agarose pad that allows for long-term single-cell microscopy observation in a stable environment. We apply this method to characterize the lag phase and persistence of individual . cells.作者: 上流社會(huì) 時(shí)間: 2025-3-26 00:24 作者: 高腳酒杯 時(shí)間: 2025-3-26 05:35
Analyzing Persister Proteomes with SILAC and Label-Free Methodsin persister cells, which are isolated by treatment with beta-lactam antibiotics. Furthermore, persister proteomes during the postantibiotic recovery phase are analyzed by label-free quantification. The presented methods are valuable tools to shed light on persister physiology.作者: 兇殘 時(shí)間: 2025-3-26 09:31 作者: ornithology 時(shí)間: 2025-3-26 13:05
https://doi.org/10.1007/978-3-531-92015-3subsequent microscopic analyses. We here describe a protocol to enrich persisters in a culture using β-lactam-induced filamentation followed by size separation. This protocol minimizes the amount of cell debris in the final sample, facilitating single-cell studies of persister cells.作者: 果核 時(shí)間: 2025-3-26 19:52 作者: 傻瓜 時(shí)間: 2025-3-26 22:16
Enrichment of Persister Cells Through Β-Lactam-Induced Filamentation and Size Separationsubsequent microscopic analyses. We here describe a protocol to enrich persisters in a culture using β-lactam-induced filamentation followed by size separation. This protocol minimizes the amount of cell debris in the final sample, facilitating single-cell studies of persister cells.作者: zonules 時(shí)間: 2025-3-27 04:29 作者: Blemish 時(shí)間: 2025-3-27 08:35
Instruktionsdesign und Unterrichtsplanungrsistence to antibiotics. Here, we describe methodologies to segregate bacterial populations based on chromosome number, assess the purity of those subpopulations, and suggest assays that could be used to quantify the impacts of genome abundance on persistence.作者: 誘騙 時(shí)間: 2025-3-27 10:48
Zentrale Begriffe und Konzepte der Bioethik,ion of components involved in alleviating antibiotic stress and helps to unravel specific molecular pathways leading to drug tolerance or resistance. We discuss the influence of single or double drug treatment regimens and environmental aspects on the evolution of antibiotic resilience mechanisms.作者: Comedienne 時(shí)間: 2025-3-27 14:01 作者: expunge 時(shí)間: 2025-3-27 19:12 作者: 拉開這車床 時(shí)間: 2025-3-28 00:51 作者: 催眠藥 時(shí)間: 2025-3-28 04:44
The Use of Experimental Evolution to Study the Response of , to Single or Double Antibiotic Treatmenion of components involved in alleviating antibiotic stress and helps to unravel specific molecular pathways leading to drug tolerance or resistance. We discuss the influence of single or double drug treatment regimens and environmental aspects on the evolution of antibiotic resilience mechanisms.作者: 使人入神 時(shí)間: 2025-3-28 07:01 作者: Cardioversion 時(shí)間: 2025-3-28 12:02 作者: 材料等 時(shí)間: 2025-3-28 16:03 作者: malign 時(shí)間: 2025-3-28 20:51 作者: Negotiate 時(shí)間: 2025-3-29 02:18
Studying Bacterial Persistence: Established Methods and Current Advancestechniques. In this chapter, we provide an overview of well-established methods in the field and recent advances that have facilitated the investigation of persister cells and we highlight the challenges to be tackled in future persistence research.作者: 藐視 時(shí)間: 2025-3-29 04:22 作者: 仇恨 時(shí)間: 2025-3-29 08:38 作者: 主動(dòng) 時(shí)間: 2025-3-29 13:28 作者: 斜谷 時(shí)間: 2025-3-29 17:09 作者: 脊椎動(dòng)物 時(shí)間: 2025-3-29 23:34 作者: 外來(lái) 時(shí)間: 2025-3-30 01:38 作者: 河流 時(shí)間: 2025-3-30 06:24 作者: 逃避現(xiàn)實(shí) 時(shí)間: 2025-3-30 09:56
