作者: 刺激 時間: 2025-3-22 00:08
Crystallographic Analysis of Drug and Inhibitor-Binding Structure of RND-Type Multidrug Exporter AcrMurakami et al., Nature 443:173–179, 2006; Nakashima et al., Nature 480:565–569, 2011; Nakashima et al., Nature 500:120–126, 2013). Analyses revealed the existence of a specific mechanism to recognize numerous substrates that the multisite binding is the base of multidrug recognition rather than ind作者: BIBLE 時間: 2025-3-22 00:37
Generation of Conformation-Specific Antibody Fragments for Crystallization of the Multidrug Resistannizing the native conformation of a major facilitator superfamily (MFS)-type MDR transporter MdfA from .. We confirmed that the use of Fab fragments was efficient for stabilization of MdfA and improvement of its crystallization properties.作者: infelicitous 時間: 2025-3-22 07:00
Covalently Linked Trimers of RND (Resistance-Nodulation-Division) Efflux Transporters to Study Theirnvolved in proton relay, or in “real time” by using a protein in which one protomer contains two Cys residues on both sides of the large cleft in the periplasmic domain and then by rapidly inactivating this protomer with a methanethiosulfonate cross-linker.作者: 刪減 時間: 2025-3-22 11:39
Molecular Modeling of Multidrug Properties of Resistance Nodulation Division (RND) Transportersmics simulations and in silico free energy estimations can all together give precious insights into the processes of recognition and extrusion of substrates, as well as on the possible inhibition strategies.作者: 膽汁 時間: 2025-3-22 14:59 作者: 粗魯?shù)娜?nbsp; 時間: 2025-3-22 20:08
https://doi.org/10.1007/978-3-658-19558-8nizing the native conformation of a major facilitator superfamily (MFS)-type MDR transporter MdfA from .. We confirmed that the use of Fab fragments was efficient for stabilization of MdfA and improvement of its crystallization properties.作者: 脆弱帶來 時間: 2025-3-22 23:31 作者: 輕快來事 時間: 2025-3-23 02:59
https://doi.org/10.1007/978-3-476-05869-0mics simulations and in silico free energy estimations can all together give precious insights into the processes of recognition and extrusion of substrates, as well as on the possible inhibition strategies.作者: Graphite 時間: 2025-3-23 09:12 作者: GUILE 時間: 2025-3-23 11:07
https://doi.org/10.1007/978-3-476-04735-9aka et al.. Nature 496:247–251, 2013). In this chapter, we describe methodologies of the screening and synthesis of macrocyclic peptides as inhibitors, as well as the purification, crystallization, and structure determination of the complexes of the MATE-type multidrug exporter with its inhibitors.作者: minimal 時間: 2025-3-23 14:24
Crystallographic Analysis of MATE-Type Multidrug Exporter with Its Inhibitorsaka et al.. Nature 496:247–251, 2013). In this chapter, we describe methodologies of the screening and synthesis of macrocyclic peptides as inhibitors, as well as the purification, crystallization, and structure determination of the complexes of the MATE-type multidrug exporter with its inhibitors.作者: NEXUS 時間: 2025-3-23 19:26 作者: 根除 時間: 2025-3-23 23:13
1064-3745 ucible results.Contains key notes and implementation advice .This detailed volume utilizes our current understanding of the structural basis of multidrug recognition and multidrug efflux mechanisms to provide protocols involving these vital intrinsic membrane proteins widely distributed in bacteria.作者: RACE 時間: 2025-3-24 05:45
https://doi.org/10.1007/978-3-476-04735-9 we present our protocol and methodology for crystallizing the CusBA adaptor–transporter complex of .. Using these procedures, we were able to produce the first co-crystal structure of a resistance-nodulation-cell division (RND) transporter in complex with its associated membrane fusion protein.作者: Accord 時間: 2025-3-24 09:07
https://doi.org/10.1007/978-3-658-19558-8chanistic picture of this highly dynamical, multicomponent, efflux system. We describe protocols for the reconstitution and the in vitro study of transporters belonging to RND and ABC superfamilies: the AcrAB–TolC and MacAB–TolC efflux systems from . and the MexAB–OprM efflux pump from .作者: 可憎 時間: 2025-3-24 13:20 作者: Foolproof 時間: 2025-3-24 16:19
Biochemical Reconstitution and Characterization of Multicomponent Drug Efflux Transporterschanistic picture of this highly dynamical, multicomponent, efflux system. We describe protocols for the reconstitution and the in vitro study of transporters belonging to RND and ABC superfamilies: the AcrAB–TolC and MacAB–TolC efflux systems from . and the MexAB–OprM efflux pump from .作者: deciduous 時間: 2025-3-24 21:08
Alternsforschung: Entwicklung und Tendenzennd purification of the inner-membrane multidrug transporter AcrB and of DARPins, which are used for crystallization of the AcrB/DARPin complex, resulting in high-resolution diffraction and subsequent structure determination.作者: Enthralling 時間: 2025-3-24 23:18
Book 2018adily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.. .Authoritative and practical,?.Bacterial Multidrug Exporters: Methods and Protocols .serves as an ideal guide to. this fastmoving field of study..作者: fibroblast 時間: 2025-3-25 03:54 作者: 碎石 時間: 2025-3-25 10:28
Crystallographic Analysis of Drug and Inhibitor-Binding Structure of RND-Type Multidrug Exporter Acrm in cellular level. These pumps not only confer multidrug resistance of cancer cells and pathogenic bacteria but also cause hereditary diseases through the mutation. Our purposes are to elucidate the molecular structures and mechanisms of these xenobiotic exporters..We had succeeded to determine th作者: rheumatology 時間: 2025-3-25 14:46 作者: Basilar-Artery 時間: 2025-3-25 16:54 作者: 缺陷 時間: 2025-3-25 22:52
Purification of AcrAB-TolC Multidrug Efflux Pump for Cryo-EM Analysisdrug transporter AcrAB-TolC, which uses proton electrochemical gradients to vectorially drive the efflux of drugs from the cell, spans this envelope. We describe here details of the methods used to prepare the recombinant tripartite assembly for high-resolution structure determination by cryo-EM.作者: goodwill 時間: 2025-3-26 02:33
NMR Spectroscopy Approach to Study the Structure, Orientation, and Mechanism of the Multidrug Exportance to the organism. This chapter outlines the sample preparation and optimization of oriented solid-state NMR experiments applied to the study of structure and dynamics for the model transporter EmrE from the small multidrug resistance (SMR) family.作者: gnarled 時間: 2025-3-26 06:00 作者: maintenance 時間: 2025-3-26 08:45 作者: 過分自信 時間: 2025-3-26 16:29 作者: organism 時間: 2025-3-26 18:59
Determining Ligand Path Through a Major Drug Transporter, AcrB, in ,theoretical considerations. The protocols for bacterial culture preparation, membrane protein purification, fluorescent assay standardization, data collection, and data analysis are provided. Useful software tools are recommended.作者: Middle-Ear 時間: 2025-3-26 23:16
Molecular Modeling of Multidrug Properties of Resistance Nodulation Division (RND) Transportersn Gram-negative bacteria. Structural information on AcrAB-TolC and MexAB-OprM, major efflux pumps of . and . respectively, boosted intensive research aimed at understanding the molecular mechanisms ruling the active extrusion processes. In particular, several studies were devoted to the understandin作者: Mystic 時間: 2025-3-27 01:32
https://doi.org/10.1007/978-1-4939-7454-2Multidrug-resistant pathogens; Bacterial tolerance against antibiotics; Drug resistance; Crystal struct作者: CREEK 時間: 2025-3-27 06:36 作者: enlist 時間: 2025-3-27 10:07
Akihito Yamaguchi,Kunihiko NishinoIncludes cutting-edge methods and protocols for the study of bacterial multidrug exporters.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice 作者: mendacity 時間: 2025-3-27 17:12
Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/180302.jpg作者: Guaff豪情痛飲 時間: 2025-3-27 20:18
Alternsforschung: Entwicklung und Tendenzen sample quality is the most important parameter that should be addressed. In almost every case, highly pure, monodisperse, and stable protein sample is a prerequisite. Vapor diffusion is in general the method of choice for obtaining crystals. Here, we discuss a detailed protocol for overproduction a作者: Annotate 時間: 2025-3-27 22:24
Allmacht, Allwissenheit und Allgütem in cellular level. These pumps not only confer multidrug resistance of cancer cells and pathogenic bacteria but also cause hereditary diseases through the mutation. Our purposes are to elucidate the molecular structures and mechanisms of these xenobiotic exporters..We had succeeded to determine th作者: 消極詞匯 時間: 2025-3-28 05:00 作者: WAX 時間: 2025-3-28 06:51
https://doi.org/10.1007/978-3-476-04735-9on of this technique is not straightforward and often hampered by the difficulties associated with expression, purification, and crystallization. Here we present our protocol and methodology for crystallizing the CusBA adaptor–transporter complex of .. Using these procedures, we were able to produce作者: 善辯 時間: 2025-3-28 12:40 作者: 巨碩 時間: 2025-3-28 18:19
Thomas Meyer,Esther Lea Neuhannance to the organism. This chapter outlines the sample preparation and optimization of oriented solid-state NMR experiments applied to the study of structure and dynamics for the model transporter EmrE from the small multidrug resistance (SMR) family.作者: 必死 時間: 2025-3-28 19:34
https://doi.org/10.1007/978-3-658-19558-8ed not only to the difficulty of obtaining functionally active protein in mg amounts but also to the intrinsic flexibility of its multiple conformations. The cocrystallization of membrane proteins with antibody fragments has been reported as an effective approach to improve the diffraction quality o作者: chemoprevention 時間: 2025-3-28 23:02 作者: 咆哮 時間: 2025-3-29 07:06
Inklusion/Teilhabe und Anerkennungxist as homotrimers, and each protomer was proposed to undergo a cycle of conformational changes in succession so that at any given time the trimer would contain three protomers of different conformations, the functionally rotating mechanism of transport. This mechanism implies that the inactivation作者: Hyperalgesia 時間: 2025-3-29 07:42
Thomas Meyer,Esther Lea Neuhanntheoretical considerations. The protocols for bacterial culture preparation, membrane protein purification, fluorescent assay standardization, data collection, and data analysis are provided. Useful software tools are recommended.作者: Entrancing 時間: 2025-3-29 12:31 作者: Baffle 時間: 2025-3-29 17:31
Purification of AcrAB-TolC Multidrug Efflux Pump for Cryo-EM Analysisdrug transporter AcrAB-TolC, which uses proton electrochemical gradients to vectorially drive the efflux of drugs from the cell, spans this envelope. We describe here details of the methods used to prepare the recombinant tripartite assembly for high-resolution structure determination by cryo-EM.作者: 極小量 時間: 2025-3-29 23:34
NMR Spectroscopy Approach to Study the Structure, Orientation, and Mechanism of the Multidrug Exportance to the organism. This chapter outlines the sample preparation and optimization of oriented solid-state NMR experiments applied to the study of structure and dynamics for the model transporter EmrE from the small multidrug resistance (SMR) family.作者: 支形吊燈 時間: 2025-3-30 02:47
Determining Ligand Path Through a Major Drug Transporter, AcrB, in ,theoretical considerations. The protocols for bacterial culture preparation, membrane protein purification, fluorescent assay standardization, data collection, and data analysis are provided. Useful software tools are recommended.作者: Feedback 時間: 2025-3-30 05:49 作者: installment 時間: 2025-3-30 11:18
