作者: Ceramic 時(shí)間: 2025-3-21 21:28
Analyzing Mouse B Cell Responses Specific to LCMV Infection,center (GC) B cells and follicular helper CD4 T (T.) cells from the spleens and lymph nodes via flow cytometry, titration of LCMV-specific IgG in the serum after LCMV infection using an enzyme-linked immunosorbent assay (ELISA) analysis, and detection of LCMV-IgG secreted plasma cells from bone marr作者: 陰郁 時(shí)間: 2025-3-22 03:41
Imaging the Interactions Between B Cells and Antigen-Presenting Cells,on including cytoskeletal reorganization, BCR microcluster coalescence, BCR-mediated antigen gathering, and BCR signaling. Here, we provide protocols for expressing these model antigens on the surface of Cos-7 cells, transfecting B cells with siRNAs or with?plasmids encoding fluorescent proteins, us作者: 結(jié)構(gòu) 時(shí)間: 2025-3-22 07:59 作者: resuscitation 時(shí)間: 2025-3-22 12:21 作者: 使尷尬 時(shí)間: 2025-3-22 13:24
Cristina M. Rosell,Raquel Garzoncenter (GC) B cells and follicular helper CD4 T (T.) cells from the spleens and lymph nodes via flow cytometry, titration of LCMV-specific IgG in the serum after LCMV infection using an enzyme-linked immunosorbent assay (ELISA) analysis, and detection of LCMV-IgG secreted plasma cells from bone marr作者: Credence 時(shí)間: 2025-3-22 19:23
Kristina Norman PhD, MSc,Matthias Pirlich MDon including cytoskeletal reorganization, BCR microcluster coalescence, BCR-mediated antigen gathering, and BCR signaling. Here, we provide protocols for expressing these model antigens on the surface of Cos-7 cells, transfecting B cells with siRNAs or with?plasmids encoding fluorescent proteins, us作者: 疼死我了 時(shí)間: 2025-3-22 22:19
Refrigeration and Freezing Equipment,w imaging window, and a shallow depth of imaging. We have found that supplementing intravital TP-LSM with conventional confocal microscopy using thick LN sections helps to overcome some of these shortcomings. Here, we describe procedures for visualizing the behavior and trafficking of fluorescently 作者: alabaster 時(shí)間: 2025-3-23 01:58 作者: considerable 時(shí)間: 2025-3-23 08:56 作者: 下級(jí) 時(shí)間: 2025-3-23 10:34
Cristina M. Rosell,Raquel Garzonanding the basic principles of the B cell responses during viral infection is of substantial importance for anti-viral vaccine development. In inbred mice, lymphocytic choriomeningitis virus (LCMV) infection elicits robust and typical T cell-dependent B cell responses, including germinal center reac作者: indicate 時(shí)間: 2025-3-23 16:57
General Properties of Major Food Componentsmemory cells and plasma cells. Expressing genes in altered versions in primary B cells and B cell lines is an important approach to understanding how B cell receptor signaling leads to B cell activation and differentiation. Recombinant retrovirus-based transduction is the most efficient method to de作者: COLON 時(shí)間: 2025-3-23 19:50
Chemical Composition of Beverages and Drinkswith antigen leads to cell activation, which induces profound changes in the cell including cytoskeleton remodeling and membrane deformation. During this process, receptors and signaling molecules undergo spatiotemporal reorganization to form signaling microclusters and the immunological synapse. Th作者: 不可知論 時(shí)間: 2025-3-23 23:45 作者: 核心 時(shí)間: 2025-3-24 06:08
Food Fortification Policy in Canada analysis, quantitative information about cell biological parameters, such as percentage of cells dividing, time of division, and time of death, can be produced. Here, we describe the method to generate quantitative cell biological insights from dye-dilution experiments. We describe experimental met作者: Insatiable 時(shí)間: 2025-3-24 08:30
Fortification of Corn Flour-Derived Productsese pathogens. The physiology of B cells is intimately connected with the function of the B cell antigen receptor (BCR). Upon activation of BCR, transmembrane signals are generated, and several downstream pathways are activated, which provide a primary directive for the cell’s subsequent response. m作者: 老巫婆 時(shí)間: 2025-3-24 13:24
https://doi.org/10.1007/978-1-4614-7076-2ntibody responses, the proliferation and differentiation of antigen-primed B cells essentially rely on the helper function from CD4. T cells. Follicular helper T (Tfh) cells constitute a specialized Th subset that localizes in close proximity to B cells and supports B cell proliferation and differen作者: 公社 時(shí)間: 2025-3-24 18:36 作者: fiscal 時(shí)間: 2025-3-24 19:43
Kristina Norman PhD, MSc,Matthias Pirlich MDantigens to the B cell receptor (BCR) causes rapid cytoskeleton-dependent changes in the spatial organization of the BCR and other B cell membrane proteins, leading to the formation of an immune synapse. This process has been modeled using antigens attached to artificial planar lipid bilayers or to 作者: Incise 時(shí)間: 2025-3-24 23:27
Saskia J. M. Osendarp,Lynnette M. Neufeldnsformed our understanding of the “how, when and where” B lymphocytes find antigen at secondary lymphoid organs in the last 10?years. Here, I described one of these experimental models, which uses highly fluorescent particulate antigen and B cell receptor (BCR)-transgenic B cells labeled with long-t作者: 世俗 時(shí)間: 2025-3-25 06:22
George Saravacos,Athanasios E. Kostaropouloselocalization of the BCR is thus likely to provide new clues to understand the activation of the BCR. Indeed, with the in situ Fab proximity ligation assay (Fab-PLA), we now obtain proofs for the dissociation activation of BCRs and start to gain insight into how the relocalization of B cell surface 作者: Narrative 時(shí)間: 2025-3-25 08:20 作者: 畢業(yè)典禮 時(shí)間: 2025-3-25 13:12
Refrigeration and Freezing Equipment, B cell biology field TP-LSM has detailed the movement of B cells in high endothelial venules and during their transmigration into lymph organs; described the movement and positioning of B cells within lymphoid organs; outlined the mechanisms by which antigen is delivered to B cells; observed B cell作者: novelty 時(shí)間: 2025-3-25 16:59 作者: organism 時(shí)間: 2025-3-25 20:22 作者: Mirage 時(shí)間: 2025-3-26 04:13 作者: BROOK 時(shí)間: 2025-3-26 07:08 作者: 連鎖,連串 時(shí)間: 2025-3-26 10:59 作者: 改良 時(shí)間: 2025-3-26 15:46
Chaohong LiuIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: 轉(zhuǎn)向 時(shí)間: 2025-3-26 20:43 作者: Pantry 時(shí)間: 2025-3-26 23:49 作者: 戰(zhàn)役 時(shí)間: 2025-3-27 02:47
978-1-4939-8497-8Springer Science+Business Media, LLC 2018作者: 態(tài)度暖昧 時(shí)間: 2025-3-27 07:44
Activation-Induced Cytidine Deaminase Aided In Vitro Antibody Evolution,n induce other mutations, and plays a central role in introducing diversification of the antibody repertoire in B cells. Ectopic expression of AID in bacteria and non-B cells can also lead to frequent mutations in highly expressed genes. Taking advantage of this feature of AID, in recent years, syst作者: cliche 時(shí)間: 2025-3-27 10:48
Analyzing Mouse B Cell Responses Specific to LCMV Infection,anding the basic principles of the B cell responses during viral infection is of substantial importance for anti-viral vaccine development. In inbred mice, lymphocytic choriomeningitis virus (LCMV) infection elicits robust and typical T cell-dependent B cell responses, including germinal center reac作者: ARK 時(shí)間: 2025-3-27 16:27
Expression of Exogenous Genes in Murine Primary B Cells and B Cell Lines Using Retroviral Vectors,memory cells and plasma cells. Expressing genes in altered versions in primary B cells and B cell lines is an important approach to understanding how B cell receptor signaling leads to B cell activation and differentiation. Recombinant retrovirus-based transduction is the most efficient method to de作者: 啪心兒跳動(dòng) 時(shí)間: 2025-3-27 20:34 作者: Mere僅僅 時(shí)間: 2025-3-28 01:50
DNA-Based Probes for Measuring Mechanical Forces in Cell-Cell Contacts: Application to B Cell Antigthe B cell receptor (BCR), which recognizes and binds antigen displayed on the surface of an antigen-presenting cell (APC). To acquire the antigen, B cells use myosin contractility to physically pull BCR-antigen clusters from the APC membrane. These mechanical forces influence association and dissoc作者: mechanism 時(shí)間: 2025-3-28 05:28 作者: IST 時(shí)間: 2025-3-28 08:22 作者: 細(xì)菌等 時(shí)間: 2025-3-28 12:42 作者: 假 時(shí)間: 2025-3-28 14:39 作者: 難管 時(shí)間: 2025-3-28 19:46 作者: HACK 時(shí)間: 2025-3-28 23:16 作者: Esophagitis 時(shí)間: 2025-3-29 06:31 作者: Compatriot 時(shí)間: 2025-3-29 07:37
Single-Particle Tracking of Cell Surface Proteins,cell surface proteins. These studies provide fundamental insights into the regulation of membrane receptor activation and the assembly of signaling clusters. Here, we describe a method to label and track B cell receptor (BCR) and other cell surface proteins and how this method can be adapted to simu作者: 清楚 時(shí)間: 2025-3-29 13:24 作者: instate 時(shí)間: 2025-3-29 17:11
,Time-Lapse F?rster Resonance Energy Transfer Imaging by Confocal Laser Scanning Microscopy for AnalET imaging by the sensitized acceptor emission method has been widely applied to study the dynamical association between two molecules at a nanometer scale in live cells. Here, we provide a detailed protocol for FRET imaging by sensitized emission using a confocal laser scanning microscope to analyz作者: vasospasm 時(shí)間: 2025-3-29 21:53 作者: 責(zé)怪 時(shí)間: 2025-3-30 01:53 作者: Accord 時(shí)間: 2025-3-30 07:30
Visualization and Quantitative Analysis of the Actin Cytoskeleton Upon B Cell Activation,ymerization of actin monomers into filaments provides the force required for B cell spreading on the antigen-presenting cell (APC). Interestingly, the actin network also participates in cellular signaling at multiple levels. Fluorescence microscopy plays a critical role in furthering our understandi作者: 北極熊 時(shí)間: 2025-3-30 11:44 作者: 平淡而無(wú)味 時(shí)間: 2025-3-30 15:25 作者: 虛假 時(shí)間: 2025-3-30 18:20 作者: brassy 時(shí)間: 2025-3-31 00:25 作者: 鎮(zhèn)壓 時(shí)間: 2025-3-31 01:50
Expression of Exogenous Genes in Murine Primary B Cells and B Cell Lines Using Retroviral Vectors,B cell receptor signaling leads to B cell activation and differentiation. Recombinant retrovirus-based transduction is the most efficient method to deliver exogenous genes for expression in B cells. In this chapter, we describe streamlined protocols for using recombinant retroviral vectors to transduce both murine primary B cells and B cell lines.作者: APNEA 時(shí)間: 2025-3-31 06:49
In Vivo Tracking of Particulate Antigen Localization and Recognition by B Lymphocytes at Lymph Noded one of these experimental models, which uses highly fluorescent particulate antigen and B cell receptor (BCR)-transgenic B cells labeled with long-term fluorescent probes, combined with confocal and multiphoton microscopy.作者: leniency 時(shí)間: 2025-3-31 10:06
Study B Cell Antigen Receptor Nano-Scale Organization by In Situ Fab Proximity Ligation Assay,assay (Fab-PLA), we now obtain proofs for the dissociation activation of BCRs and start to gain insight into how the relocalization of B cell surface signaling molecules could activate the cells. This chapter describes our methods to study the nano-scale organization of B cell surface receptors and co-receptors with Fab-PLA.
