標(biāo)題: Titlebook: Atherosclerosis; Methods and Protocol Dipak Ramji Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license to Spr [打印本頁(yè)] 作者: 助手 時(shí)間: 2025-3-21 17:01
書(shū)目名稱(chēng)Atherosclerosis影響因子(影響力)
作者: Original 時(shí)間: 2025-3-21 22:44
Die Entwicklung der Frauenliteraturproliferate, taking up the available lipids to become foam cells and producing extracellular matrix proteins such as collagen and elastin. Plaque progression is characterized by the formation of a fibrous cap composed of extracellular matrix proteins and smooth muscle cells, which acts to stabilize 作者: SAGE 時(shí)間: 2025-3-22 02:02
Rechtspolitik als Frauenpolitik,ication of classical proliferative markers such as phopsho-Ser807/811 retinoblastoma (Rb), proliferating cell nuclear antigen (PCNA) and cyclin D1 by Western blotting. As these markers are involved in different aspects of the cell cycle regulation, examining their expression levels can not only reve作者: 原告 時(shí)間: 2025-3-22 07:45
https://doi.org/10.1007/978-3-663-10102-4 to the understanding of the process but also in providing numerous serendipitous discoveries and methodologies. In this chapter, we outline the methodologies for the preparation and testing of various lipoproteins for oxidation studies.作者: hangdog 時(shí)間: 2025-3-22 11:45 作者: 胡言亂語(yǔ) 時(shí)間: 2025-3-22 16:14
Frauenportr?ts und -protokolle aus der DDR1,6-diphenyl-1,3,5-hexatriene; DPH) that mimic the molecular movements of the sample analyzed. Since . refers to the rigidity and fluidity refers to the viscosity of lipid layers, the fluidity index is the inverse value of . (i.e., 1/.). This chapter describes a method for measuring HDL monolayer fl作者: 博識(shí) 時(shí)間: 2025-3-22 18:02 作者: 傻瓜 時(shí)間: 2025-3-22 21:15 作者: 殘酷的地方 時(shí)間: 2025-3-23 03:12 作者: 疏忽 時(shí)間: 2025-3-23 07:53
A Fluorescence-Based In Vitro Method to Assess Cholesterol Efflux), HDL. and HDL. subfractions, serum, plasma or ApoB-depleted serum or plasma. While lipid-free ApoA-1 mediates ChE via only ATP-binding cassette (ABC)A1 transporter, the remaining acceptors mediate ChE via ABCA1, ABCG1 and scavenger receptor class B type 1 (SRB1) transporters. The reproducibility o作者: 跳脫衣舞的人 時(shí)間: 2025-3-23 13:40 作者: 可以任性 時(shí)間: 2025-3-23 17:16
Atherosclerosis: Pathogenesis and Key Cellular Processes , Current and Emerging Therapies , Key Chal about 31% of all global deaths. Atherogenesis is influenced by a range of factors, including oxidative stress, inflammation, hypertension, and hyperlipidemia, and is ultimately driven by the accumulation of low-density lipoprotein cholesterol within the arterial wall of medium and large arteries. L作者: CLAN 時(shí)間: 2025-3-23 21:38 作者: CAPE 時(shí)間: 2025-3-23 22:25 作者: 爆炸 時(shí)間: 2025-3-24 03:17 作者: AGONY 時(shí)間: 2025-3-24 06:55
Exploring the Associations Between Clonal Hematopoiesis of Indeterminate Potential , Myeloid Maligna a potential forerunner to hematologic malignancy or as a clinically silent phase in leukemia that antedates symptomatic disease. That definition of clonal hematopoiesis has now been expanded to encompass patients who harbor specific genetic/epigenetic mutations that lead to clonal hematopoiesis of 作者: Cupidity 時(shí)間: 2025-3-24 13:01 作者: MEEK 時(shí)間: 2025-3-24 16:15 作者: PLUMP 時(shí)間: 2025-3-24 19:10
Effective Transfection and Gene Silencing of Primary Murine Macrophages with Small Interfering RNAllular intrusion. The introduction of small interfering RNA (siRNA) encapsulated in liposomes to the cell to decrease gene expression is one of the methods that can be used to achieve gene silencing. There are different commercially available compounds to introduce siRNA into the cell, including Lip作者: cardiac-arrest 時(shí)間: 2025-3-25 00:17 作者: Gobble 時(shí)間: 2025-3-25 06:10
Monocyte Adhesion Assays for Detecting Endothelial Cell Activation in Vascular Inflammation and Athes in vitro and measures EC activation. We describe in this chapter a monocyte adhesion assay based on two published papers from our laboratory that can be effectively used in studying the mechanisms of both pro- and anti-inflammatory cytokines in EC activation. Endothelial cell monolayers are cultur作者: 簡(jiǎn)潔 時(shí)間: 2025-3-25 09:13
Chick Embryo Ex Vivo Aortic Sprouting Assays for Cardiovascular Researchan be replicated in vitro under chemically specified conditions by cultivating chick aortic explants in biomatrices. In this technique, angiogenesis is powered by endogenous molecules that the aorta releases to promote its outgrowth. In an ordered series of morphogenetic events, sprouting endothelia作者: 我們的面粉 時(shí)間: 2025-3-25 11:57
Purification and Analysis of Circulating Lipid Particles particles provide essential lipids and fats which enable cells to synthesize new membranes and regulate different biochemical pathways. Imbalance in lipid particle metabolism can cause pathological states such as atherosclerosis. Here, elevated low-density lipoprotein (LDL) accumulation leads to fa作者: Intruder 時(shí)間: 2025-3-25 18:46 作者: Endearing 時(shí)間: 2025-3-25 22:10 作者: 我不怕?tīng)奚?nbsp; 時(shí)間: 2025-3-26 00:21 作者: ALIBI 時(shí)間: 2025-3-26 08:03
Monitoring the Anisotropy and Fluidity of the HDL Monolayer as Surrogates of HDL Functionalityis considered as a surrogate of HDL functionality. In particular, the more fluid the HDL monolayer is, the greater the cholesterol efflux (ChE) is observed. Fluidity depends on cholesterol and on the saturation and length of the fatty acids present in lipid layers. Specifically, low cholesterol and 作者: absorbed 時(shí)間: 2025-3-26 10:26
Assessment of Ex Vivo Antioxidative Potential of Murine HDL in Atherosclerosisinciple of the method is based on the rearrangement of double bonds of polyunsaturated fatty acids that occurs during the oxidation of human LDL, which generates a sigmoidal curve. The shape and length of the curve is modified in the presence of HDL, and such modifications are easily quantifiable by作者: ILEUM 時(shí)間: 2025-3-26 16:43 作者: MAIM 時(shí)間: 2025-3-26 19:50 作者: anaerobic 時(shí)間: 2025-3-27 00:25 作者: Prognosis 時(shí)間: 2025-3-27 03:31 作者: cultivated 時(shí)間: 2025-3-27 07:03
Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/163945.jpg作者: 相同 時(shí)間: 2025-3-27 09:50
Die Entwicklung der Frauenliteratur about 31% of all global deaths. Atherogenesis is influenced by a range of factors, including oxidative stress, inflammation, hypertension, and hyperlipidemia, and is ultimately driven by the accumulation of low-density lipoprotein cholesterol within the arterial wall of medium and large arteries. L作者: FEIGN 時(shí)間: 2025-3-27 17:39
H?ndlerinnen-Assoziationen der Yorubaesponse and in atherosclerosis, the balance of anti- and pro-inflammatory cytokines is tipped in favor of the latter, resulting in persistent and unresolved inflammation. Although reducing plasma cholesterol levels mainly via the use of statins has positively impacted patient outcomes and reduced mo作者: 著名 時(shí)間: 2025-3-27 21:24
Frauenzusammenschlüsse der Mendenction, chemokine-driven recruitment of immune cells, differentiation of monocytes to macrophages and their subsequent transformation into lipid laden foam cells, activation of inflammasome and pro-inflammatory signaling, and migration of smooth muscle cells from the media to the intima. The use of 作者: Conduit 時(shí)間: 2025-3-28 01:11 作者: 含水層 時(shí)間: 2025-3-28 06:03
Der sozialp?dagogische Blick auf Familie a potential forerunner to hematologic malignancy or as a clinically silent phase in leukemia that antedates symptomatic disease. That definition of clonal hematopoiesis has now been expanded to encompass patients who harbor specific genetic/epigenetic mutations that lead to clonal hematopoiesis of 作者: 奇思怪想 時(shí)間: 2025-3-28 07:17
Einleitung: Frauenpolitik in Familienhand?ce homology to any of the exchangeable apolipoproteins. Based on the apolipoprotein A-I (the major protein component of high density lipoproteins, HDL) mimicking properties, they were termed as ApoA-I mimicking peptides. Several laboratories around the world started studying such de novo-designed pe作者: 最后一個(gè) 時(shí)間: 2025-3-28 13:41
https://doi.org/10.1007/978-3-531-92200-3en phagocytosis. However, in recent years, several new functions such as angiogenesis, tissue remodeling, clearance of apoptotic cells, pro- and anti-inflammatory properties and tumor growth have been attributed to macrophages. To perform such varied functions, macrophages acquire specific phenotype作者: Cirrhosis 時(shí)間: 2025-3-28 15:33 作者: deactivate 時(shí)間: 2025-3-28 21:47
Rechtspolitik als Frauenpolitik,of the cell cycle is used to quantify cell proliferation. Quantification of incorporated BrdU is considered the most direct measure of cell proliferation, and here we describe BrdU incorporation into cultured vascular smooth muscle cells (VSMCs) and endothelial cells in vitro. Incorporation of fluor作者: reception 時(shí)間: 2025-3-29 02:16 作者: Magisterial 時(shí)間: 2025-3-29 06:09
https://doi.org/10.1007/978-3-322-97318-4an be replicated in vitro under chemically specified conditions by cultivating chick aortic explants in biomatrices. In this technique, angiogenesis is powered by endogenous molecules that the aorta releases to promote its outgrowth. In an ordered series of morphogenetic events, sprouting endothelia作者: 間諜活動(dòng) 時(shí)間: 2025-3-29 08:40
Frauenpolitische Chancen globaler Politik particles provide essential lipids and fats which enable cells to synthesize new membranes and regulate different biochemical pathways. Imbalance in lipid particle metabolism can cause pathological states such as atherosclerosis. Here, elevated low-density lipoprotein (LDL) accumulation leads to fa作者: medium 時(shí)間: 2025-3-29 15:24 作者: POINT 時(shí)間: 2025-3-29 18:38 作者: pacifist 時(shí)間: 2025-3-29 22:36
Barbara Holland-Cunz,Uta Ruppertactor. ChE has been traditionally assessed by in vitro radioactive methods but these are not appropriate when assessing a large number of samples. Therefore, alternative, reproducible nonradioactive methods have been developed. This chapter describes a robust nonradioactive method using a fluorescen作者: 怒目而視 時(shí)間: 2025-3-30 03:36
Frauenportr?ts und -protokolle aus der DDRis considered as a surrogate of HDL functionality. In particular, the more fluid the HDL monolayer is, the greater the cholesterol efflux (ChE) is observed. Fluidity depends on cholesterol and on the saturation and length of the fatty acids present in lipid layers. Specifically, low cholesterol and 作者: 玩笑 時(shí)間: 2025-3-30 06:17
Frauenportr?ts und -protokolle aus der DDRinciple of the method is based on the rearrangement of double bonds of polyunsaturated fatty acids that occurs during the oxidation of human LDL, which generates a sigmoidal curve. The shape and length of the curve is modified in the presence of HDL, and such modifications are easily quantifiable by作者: Vertebra 時(shí)間: 2025-3-30 10:42 作者: Commission 時(shí)間: 2025-3-30 15:55 作者: 原來(lái) 時(shí)間: 2025-3-30 19:10 作者: debris 時(shí)間: 2025-3-30 22:32
Book 2022is of atherosclerosis and model systems together with?.in vitro.,?.ex vivo.,?.in vivo.?and emerging methods in atherosclerosis research. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary ma作者: 表示向前 時(shí)間: 2025-3-31 02:53
Survey of In Vitro Model Systems for Investigation of Key Cellular Processes Associated with Atherosin vitro model systems has considerably advanced our understanding of these atherosclerosis-associated processes and they are also often used in drug discovery and other screening platforms. This chapter will describe key in vitro model systems employed frequently in atherosclerosis research.作者: 斥責(zé) 時(shí)間: 2025-3-31 07:51
Survey of Approaches for Investigation of Atherosclerosis In Vivorevent plaque formation and even reverse existing disease. This chapter will provide a survey of such animal models and associated techniques that are routinely used for research of atherosclerosis in vivo.作者: Expand 時(shí)間: 2025-3-31 09:36
Assessment of Ex Vivo Antioxidative Potential of Murine HDL in Atherosclerosis measuring the absorbance of conjugated dienes at 234?nm. The general technique described herein may be applied to evaluate the effect of HDL obtained from different experimental murine models of atherosclerosis.作者: Cardiac 時(shí)間: 2025-3-31 13:34
1064-3745 ation advice from the experts.This volume provides detailed, up-to-date methods used in.?.research on Atherosclerosis. Chapters guide readers through an overview of the pathogenesis of atherosclerosis and model systems together with?.in vitro.,?.ex vivo.,?.in vivo.?and emerging methods in atheroscle作者: vibrant 時(shí)間: 2025-3-31 19:15 作者: Infusion 時(shí)間: 2025-4-1 00:19
Rechtspolitik als Frauenpolitik,thods that can be used to achieve gene silencing. There are different commercially available compounds to introduce siRNA into the cell, including Lipofectamine RNAiMAX and HiPerfect. The chapter will describe a method for gene silencing in mouse primary macrophages using liposome-based transfection of siRNA.作者: 無(wú)表情 時(shí)間: 2025-4-1 03:37
