標(biāo)題: Titlebook: Arthritis Research; Volume 1: Methods an Andrew P. Cope Book 2007 Humana Press 2007 Arthritis.PCR.gene expression.gene transfer.genes.hybri [打印本頁] 作者: misperceive 時(shí)間: 2025-3-21 17:16
書目名稱Arthritis Research影響因子(影響力)
書目名稱Arthritis Research影響因子(影響力)學(xué)科排名
書目名稱Arthritis Research網(wǎng)絡(luò)公開度
書目名稱Arthritis Research網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Arthritis Research被引頻次
書目名稱Arthritis Research被引頻次學(xué)科排名
書目名稱Arthritis Research年度引用
書目名稱Arthritis Research年度引用學(xué)科排名
書目名稱Arthritis Research讀者反饋
書目名稱Arthritis Research讀者反饋學(xué)科排名
作者: Geyser 時(shí)間: 2025-3-21 20:15
Hybridization of Synovial Tissuee, specific, and sensitive, because even small quantities of mRNA can be detected. Presented here contemporary protocols for ISH using a combined nonradioactive immunohistochemical detection system..In overview, the following steps have to be covered to perform ISH. (1) mRNA probes (sense and antise作者: 存心 時(shí)間: 2025-3-22 00:54
Subtractive Hybridization is a screening method for differentially expressed genes. At first, poly-A.-RNA is isolated and reverse transcribed into cDNA. With the SMART technology, total RNA can be used. For subtractive hybridization, two adaptors are ligated to the tester cDNA. The tester cDNA is then mixed twice with cDNA 作者: ironic 時(shí)間: 2025-3-22 05:51
Laser Capture as a Tool for Analysis of Gene Expression in Inflamed Synoviumal biopsies. However, this strategy does not distinguish between specific gene expression profiles of cells originating from discrete tissue areas. Therefore, we established the combination of laser-mediated microdissection and RNA arbitrarily primed polymerase chain reaction (RAP-PCR) for different作者: Hypopnea 時(shí)間: 2025-3-22 09:20 作者: 抒情短詩 時(shí)間: 2025-3-22 13:40 作者: 粗魯?shù)娜?nbsp; 時(shí)間: 2025-3-22 20:53 作者: 進(jìn)取心 時(shí)間: 2025-3-23 01:04
Image Analysis of Aggrecan Degradation in Articular Cartilage With Formalin-Fixed Samplesrtilage. The early histochemical/histological scale of Mankin (.) has been widely used but recently challenged as insufficient (.). Imaging techniques such as microscopic magnetic resonance imaging (MRI) (.), polarized light microscopy (.), atomic force microscopy (.), and infrared spectral analysis作者: SOW 時(shí)間: 2025-3-23 03:45 作者: 影響 時(shí)間: 2025-3-23 05:41
Measurement of Glycosaminoglycan Release from Cartilage Explantscy of different cytokines alone or in combination in simulating cartilage catabolism. Two approaches for GAG assay are described, with special attention being paid to the advantages and limitations of each method作者: interference 時(shí)間: 2025-3-23 12:05
Assessment of Gelatinase Expression and Activity in Articular Cartilagealysis of tissue sections provides information about the precise localization of the enzymes within the tissue, pinpointing the cells that synthesize the proteinases, and zymography of cell/tissue conditioned culture media allows a semi-quantitative assessment of the gelatinases and their activation作者: DENT 時(shí)間: 2025-3-23 14:38
Analyses of MT1-MMP Activity in Cellsthe characteristic functions of this enzyme is activation of proMMP-2 on the cell surface (.). This process can be monitored using gelatin zymography to detect the pro- and active forms of MMP-2. Cellular MT1-MMP activity can also be directly detected by . degradation of layers of either gelatin or 作者: SLAY 時(shí)間: 2025-3-23 18:20 作者: meretricious 時(shí)間: 2025-3-23 22:24 作者: 鋼筆記下懲罰 時(shí)間: 2025-3-24 04:02 作者: POINT 時(shí)間: 2025-3-24 09:41
Andrew P. CopeBroad range of topics covering many aspects of rheumatic disease.In-depth studies of cutting-edge technology.Robust tools for translational research.Contributions from leaders in their fields作者: 使入迷 時(shí)間: 2025-3-24 12:12
Methods in Molecular Medicinehttp://image.papertrans.cn/b/image/161918.jpg作者: 幻影 時(shí)間: 2025-3-24 15:23
Measurement of Glycosaminoglycan Release from Cartilage Explantscy of different cytokines alone or in combination in simulating cartilage catabolism. Two approaches for GAG assay are described, with special attention being paid to the advantages and limitations of each method作者: Congruous 時(shí)間: 2025-3-24 22:47 作者: 吼叫 時(shí)間: 2025-3-25 00:39 作者: Lacunar-Stroke 時(shí)間: 2025-3-25 06:27
Arthritis Research978-1-59745-401-8Series ISSN 1543-1894 Series E-ISSN 1940-6037 作者: BLOT 時(shí)間: 2025-3-25 09:48
https://doi.org/10.1007/978-0-585-30665-0cy of different cytokines alone or in combination in simulating cartilage catabolism. Two approaches for GAG assay are described, with special attention being paid to the advantages and limitations of each method作者: 沒花的是打擾 時(shí)間: 2025-3-25 12:28 作者: 改進(jìn) 時(shí)間: 2025-3-25 19:49 作者: laceration 時(shí)間: 2025-3-25 21:00
Verbal and Non-verbal Communication,Assay of collagenase activity involves the use of radiolabeled collagen. Stimulation of cartilage with proinflammatory cytokines results in the upregulation of collagenases and the subsequent release of degraded collagen fragments. These enzymes can be localized in both osteoarthritic and rheumatoid arthritis cartilage and synovial tissues.作者: Endoscope 時(shí)間: 2025-3-26 02:17 作者: 欲望小妹 時(shí)間: 2025-3-26 07:29 作者: Palpate 時(shí)間: 2025-3-26 09:21 作者: 進(jìn)步 時(shí)間: 2025-3-26 15:47
Verbal and Non-verbal Communication, is a screening method for differentially expressed genes. At first, poly-A.-RNA is isolated and reverse transcribed into cDNA. With the SMART technology, total RNA can be used. For subtractive hybridization, two adaptors are ligated to the tester cDNA. The tester cDNA is then mixed twice with cDNA 作者: 神秘 時(shí)間: 2025-3-26 17:31
Heredity, Environment and Intelligence,al biopsies. However, this strategy does not distinguish between specific gene expression profiles of cells originating from discrete tissue areas. Therefore, we established the combination of laser-mediated microdissection and RNA arbitrarily primed polymerase chain reaction (RAP-PCR) for different作者: 平靜生活 時(shí)間: 2025-3-26 23:07 作者: overreach 時(shí)間: 2025-3-27 02:19 作者: 后退 時(shí)間: 2025-3-27 08:06
https://doi.org/10.1007/978-0-585-30665-0medicine. Investigators now appreciate the need to gauge pathological changes and their response to treatment by quantifying susceptible structural parameters. But the desired information respecting three-dimensional structures must be gleaned from either actual or virtual two-dimensional sections t作者: AFFIX 時(shí)間: 2025-3-27 12:01
Heredity, Environment and Intelligence,rtilage. The early histochemical/histological scale of Mankin (.) has been widely used but recently challenged as insufficient (.). Imaging techniques such as microscopic magnetic resonance imaging (MRI) (.), polarized light microscopy (.), atomic force microscopy (.), and infrared spectral analysis作者: 門窗的側(cè)柱 時(shí)間: 2025-3-27 15:24 作者: Vertebra 時(shí)間: 2025-3-27 18:56
https://doi.org/10.1007/978-0-585-30665-0cy of different cytokines alone or in combination in simulating cartilage catabolism. Two approaches for GAG assay are described, with special attention being paid to the advantages and limitations of each method作者: 阻止 時(shí)間: 2025-3-28 00:58
https://doi.org/10.1007/978-0-585-30665-0alysis of tissue sections provides information about the precise localization of the enzymes within the tissue, pinpointing the cells that synthesize the proteinases, and zymography of cell/tissue conditioned culture media allows a semi-quantitative assessment of the gelatinases and their activation作者: 騷動(dòng) 時(shí)間: 2025-3-28 02:34 作者: ELUC 時(shí)間: 2025-3-28 08:17 作者: craving 時(shí)間: 2025-3-28 14:13 作者: palpitate 時(shí)間: 2025-3-28 16:33
Arthroscopy as a Research Toolrole of “gold standard” amongst currently available imaging techniques. The evolution of arthroscopy is reviewed together with an overview of the evidence obtained from its research application in the rheumatology. Methodology for the conduct of arthroscopy and synovial biopsy is described.作者: Ingratiate 時(shí)間: 2025-3-28 19:15 作者: 瑣碎 時(shí)間: 2025-3-29 02:01 作者: 放氣 時(shí)間: 2025-3-29 04:54
Verbal and Non-verbal Communication,tion of cell death is an important technique in studying articular cartilage as it most closely resembles the in vivo situation. The methods described here involve the use of light microscopy and electron microscopy in conjunction with fluorescent and biochemical methods to correctly ascertain the type of cell death that has occurred.作者: 新奇 時(shí)間: 2025-3-29 10:42 作者: 動(dòng)脈 時(shí)間: 2025-3-29 15:19 作者: 幼兒 時(shí)間: 2025-3-29 18:52
, Detection of Cell Death in Articular Cartilagetion of cell death is an important technique in studying articular cartilage as it most closely resembles the in vivo situation. The methods described here involve the use of light microscopy and electron microscopy in conjunction with fluorescent and biochemical methods to correctly ascertain the type of cell death that has occurred.作者: 江湖郎中 時(shí)間: 2025-3-29 20:49 作者: 同位素 時(shí)間: 2025-3-30 03:41 作者: OTHER 時(shí)間: 2025-3-30 04:05 作者: essential-fats 時(shí)間: 2025-3-30 10:22
Heredity, Environment and Intelligence,croscope. RNA was isolated and analyzed using nested RNA arbitrarily primed PCR to generate a fingerprint of the expressed gene sequences. Differentially expressed bands were isolated, cloned, and sequenced. Differential expression of identified sequences was confirmed by . hybridization and immunohistochemistry.作者: AGATE 時(shí)間: 2025-3-30 15:31 作者: 細(xì)微的差異 時(shí)間: 2025-3-30 19:02
Laser Capture as a Tool for Analysis of Gene Expression in Inflamed Synoviumcroscope. RNA was isolated and analyzed using nested RNA arbitrarily primed PCR to generate a fingerprint of the expressed gene sequences. Differentially expressed bands were isolated, cloned, and sequenced. Differential expression of identified sequences was confirmed by . hybridization and immunohistochemistry.作者: 易受騙 時(shí)間: 2025-3-30 22:52 作者: 藥物 時(shí)間: 2025-3-31 02:13 作者: 統(tǒng)治人類 時(shí)間: 2025-3-31 06:48 作者: 做作 時(shí)間: 2025-3-31 11:52 作者: 辯論的終結(jié) 時(shí)間: 2025-3-31 15:37
