作者: ATP861 時間: 2025-3-21 20:22
Prevention and Control of Pests and Diseasesthe plants outright, insect pests and microbial pathogens can cause subtle changes in gene expression or plant metabolism that affect experimental results. Therefore, regular scouting for infestations, frequent cleaning of plant growth areas, proper disposal of dead or diseased plant material, and c作者: 謙卑 時間: 2025-3-22 01:25
Growth of Other Species Related to ,otocols for growth and maintenance of these plant species. Like ., many wild crucifers are amenable to growth in greenhouses, growth rooms, and growth chambers for laboratory study. Some aspects of their care are quite similar to .. However, several unique traits, such as perennial growth habit, lon作者: Contend 時間: 2025-3-22 05:30 作者: Fretful 時間: 2025-3-22 08:44 作者: Collected 時間: 2025-3-22 14:47
Genetic Analysisis chapter. These include the determination of the number of genes involved in the observed trait’s variation, the determination of dominance relationships between alleles of the same locus, and epistatic interactions with related genetic variants. A new variant should be compared with previously id作者: aquatic 時間: 2025-3-22 18:35
QTL Analysisal, and evolutionary levels. A major step in these studies is the identification and location of quantitative trait loci (QTLs) determining the variation for trait(s) of interest, so-called QTL analysis. Here we provide general methods to achieve this goal, including (1) the selection of wild access作者: 費(fèi)解 時間: 2025-3-23 00:52
EMS Mutagenesis of ,esponses. Various approaches for mutagenesis involving chemical, irradiation, and insertional methods have been developed; each has advantages and disadvantages for the study of gene function. In this post-genomic era, the use of reverse genetic approaches to understanding the role of genes in growt作者: ANTH 時間: 2025-3-23 04:37
Low-Resolution Mapping of Untagged Mutationsy labeled oligonucleotides as primers. For the genotyping of a single plant from a mapping population, only two simultaneous amplifications are required, the products of which are finally electrophoresed in an automated DNA sequencer controlled by fragment analysis software. An analysis of the genot作者: tic-douloureux 時間: 2025-3-23 08:46 作者: ORBIT 時間: 2025-3-23 11:55 作者: 專心 時間: 2025-3-23 17:52 作者: Comedienne 時間: 2025-3-23 20:06 作者: SENT 時間: 2025-3-23 22:51
PCR-Based Screening for Insertional Mutantsction mutants in genes of interest. Many collections of T-DNA and transposon-tagged plants have been generated and several have been made available to the research community. With recent sequencing of the insertion sites and the creation of public databases, identification of knockouts in most of th作者: obligation 時間: 2025-3-24 06:23
Cytogenetic Analyses of ,cable to this model plant during the past decade. Recently, the very small genome, the low content, and strong clustering of repetitive sequences even turned out to be advantageous for the first establishment of chromosome painting in a euploid plant. Chromosome painting together with the high-resol作者: 感激小女 時間: 2025-3-24 07:17
Using Information From Public , Databases to Aid Researchs. Much of the data are stored in public databases, where data are organized, analyzed, and made freely accessible to the research community. These databases are resources that researchers can utilize for making predictions and developing testable hypotheses. The methods in this chapter describe way作者: lavish 時間: 2025-3-24 14:17
Cathleen Grunert,Karin Bock,Wolfgang Schr?erions, insecticide or fungicide spraying by a licensed applicator may be necessary. Bacterial and viral infections of ., though they do occur, tend to be less common and can usually be controlled by maintaining optimal growth conditions and promptly disposing of dead or diseased plant material.作者: OGLE 時間: 2025-3-24 17:16
Schlüsselprobleme weiter denken! is applicable both to . and the tobacco BY2 cell lines. Cell-cycle synchronization capacity of the parental lines is maintained after both transformation and recovery from cryopreservation. The techniques described here require no specialized equipment and are suitable for routine laboratory use, g作者: incontinence 時間: 2025-3-24 21:19
J?rg Dinkelaker,Matthias Herrlene fine-scale genetic linkage, ideally narrowing to a chromosomal region of about 40 kbp; and (4) sequencing of mutant and wild-type DNA is used to verify the identity of the mutated gene. Given a mutant phenotype that can be determined unambiguously in a single F2 plant, it is possible to complete 作者: 女歌星 時間: 2025-3-25 00:21
https://doi.org/10.1007/978-3-531-92362-8once again, are becoming valuable for obtaining loss-of-function alleles in the genes “missed” by the sequencing projects. In this chapter, we provide a detailed description of the design and implementation of a PCR-based screen for insertional knockouts using T-DNA-mutagenized lines as an example.作者: vanquish 時間: 2025-3-25 05:21 作者: 粘 時間: 2025-3-25 09:32
Synchronization, Transformation, and Cryopreservation of Suspension-Cultured Cells is applicable both to . and the tobacco BY2 cell lines. Cell-cycle synchronization capacity of the parental lines is maintained after both transformation and recovery from cryopreservation. The techniques described here require no specialized equipment and are suitable for routine laboratory use, g作者: 關(guān)心 時間: 2025-3-25 14:28
Gene Identification and Cloning by Molecular Marker Mappingne fine-scale genetic linkage, ideally narrowing to a chromosomal region of about 40 kbp; and (4) sequencing of mutant and wild-type DNA is used to verify the identity of the mutated gene. Given a mutant phenotype that can be determined unambiguously in a single F2 plant, it is possible to complete 作者: Manifest 時間: 2025-3-25 17:54
PCR-Based Screening for Insertional Mutantsonce again, are becoming valuable for obtaining loss-of-function alleles in the genes “missed” by the sequencing projects. In this chapter, we provide a detailed description of the design and implementation of a PCR-based screen for insertional knockouts using T-DNA-mutagenized lines as an example.作者: magenta 時間: 2025-3-25 21:25
1064-3745 . Completion of the Arabidopsis genome sequence offered for the first time the opportunity to have in hand all of the genetic information required for studying plant function. In addition, the 978-1-61737-539-2978-1-59745-003-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 喃喃訴苦 時間: 2025-3-26 00:35 作者: PALMY 時間: 2025-3-26 07:46 作者: 勉勵 時間: 2025-3-26 10:28 作者: Cosmopolitan 時間: 2025-3-26 13:17 作者: monogamy 時間: 2025-3-26 20:02
Empirische Forschung und Waldorfp?dagogikescribe standard cytogenetic methods including preparation of chromosomes and chromatin fibers, isolation of interphase nuclei, FISH and immunostaining, painting of . chromosomes, and comparative chromosome painting in other . species.作者: 固執(zhí)點好 時間: 2025-3-26 22:33
Growth of Other Species Related to ,h and good seed set. This chapter attempts to provide sources for acccessions and standard protocols for plant growth and seed harvest of wild crucifers while providing special tips for species with which we have experience.作者: Encapsulate 時間: 2025-3-27 05:06 作者: 沒收 時間: 2025-3-27 07:14
EMS Mutagenesis of ,esulfonate (EMS) mutagenesis can be used for both forward and reverse genetic studies. Generation of diverse mutant alleles in the same gene provides critical tools to understand the role of these genes in the function of the organism. Here we describe the general method of EMS mutagenesis for the molecular genetic model plant ..作者: 侵略主義 時間: 2025-3-27 09:59
Cytogenetic Analyses of ,escribe standard cytogenetic methods including preparation of chromosomes and chromatin fibers, isolation of interphase nuclei, FISH and immunostaining, painting of . chromosomes, and comparative chromosome painting in other . species.作者: 外形 時間: 2025-3-27 14:25
https://doi.org/10.1007/978-3-658-20596-6ions that can serve as parental lines for QTL mapping; (2) the development of mapping populations; (3) the construction of the required genomewide linkage maps of the populations; and (4) the mapping and characterization of QTLs affecting the trait(s).作者: integral 時間: 2025-3-27 20:28 作者: Antagonist 時間: 2025-3-27 23:48
https://doi.org/10.1007/978-3-531-92362-8 isolation of insertion/genome junctions, and the stabilization of transposon-induced mutations are provided, together with an overview of available insertional mutant collections and of their characteristics.作者: 獸皮 時間: 2025-3-28 05:10 作者: NORM 時間: 2025-3-28 09:17
Martin Heinrich,Jan Christoph St?rtl?nder now several well-established grafting procedures available, which we described here, and their use has already contributed greatly to understanding of such processes as shoot branching control, flowering, and disease resistance.作者: 馬籠頭 時間: 2025-3-28 12:09
https://doi.org/10.1007/978-3-658-20596-6ed, the products of which are finally electrophoresed in an automated DNA sequencer controlled by fragment analysis software. An analysis of the genotypes of 50 plants allows mapping of the mutation of interest within a candidate genomic interval of about 15 cM (3 Mb, corresponding to about 40 BAC clones).作者: meretricious 時間: 2025-3-28 17:47 作者: Restenosis 時間: 2025-3-28 19:12
Grafting now several well-established grafting procedures available, which we described here, and their use has already contributed greatly to understanding of such processes as shoot branching control, flowering, and disease resistance.作者: 織布機(jī) 時間: 2025-3-29 00:55 作者: dearth 時間: 2025-3-29 03:12 作者: agitate 時間: 2025-3-29 07:31
QTL Analysisions that can serve as parental lines for QTL mapping; (2) the development of mapping populations; (3) the construction of the required genomewide linkage maps of the populations; and (4) the mapping and characterization of QTLs affecting the trait(s).作者: 我怕被刺穿 時間: 2025-3-29 12:48
High-Throughput TILLING for ,re substrates for cleavage by the endonuclease CEL I. Following cleavage, products are analyzed on denaturing polyacrylamide gels using the LI-COR DNA analyzer system. High-throughput TILLING has been adopted by the . TILLING Project (ATP) to provide allelic series of point mutations for the general . community.作者: FATAL 時間: 2025-3-29 18:07
Forward Genetic Screening of Insertional Mutants isolation of insertion/genome junctions, and the stabilization of transposon-induced mutations are provided, together with an overview of available insertional mutant collections and of their characteristics.作者: harangue 時間: 2025-3-29 22:58 作者: 圓木可阻礙 時間: 2025-3-30 03:49 作者: 泥沼 時間: 2025-3-30 05:23 作者: Observe 時間: 2025-3-30 12:00 作者: 動作謎 時間: 2025-3-30 16:01
Arabidopsis Protocols, 2nd Edition978-1-59745-003-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 高興一回 時間: 2025-3-30 18:36
Erziehungswissenschaftliche Jugendforschunging, seed quality, and seed preservation are also considered. In addition, this chapter elaborates the conditions that are critical to the growth and development of healthy plants that produce high quality and quantity of seeds. The plant and seed management methods are discussed in the chronological order in which they would normally be utilized.作者: 全部逛商店 時間: 2025-3-30 23:24 作者: geometrician 時間: 2025-3-31 04:04 作者: 合同 時間: 2025-3-31 07:51
Cathleen Grunert,Karin Bock,Wolfgang Schr?erthe plants outright, insect pests and microbial pathogens can cause subtle changes in gene expression or plant metabolism that affect experimental results. Therefore, regular scouting for infestations, frequent cleaning of plant growth areas, proper disposal of dead or diseased plant material, and c
