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標(biāo)題: Titlebook: Applications of Mass Spectrometry in Microbiology; From Strain Characte Plamen Demirev,Todd R. Sandrin Book 2016 Springer International Pub [打印本頁]

作者: vein220    時間: 2025-3-21 17:12
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作者: osteopath    時間: 2025-3-21 22:14

作者: Stagger    時間: 2025-3-22 01:36
Maximizing the Taxonomic Resolution of MALDI-TOF-MS-Based Approaches to Bacterial Characterization: of counter-bioterrorism strategies, prevention of food contamination, and rapid diagnosis of bacterial infections. This chapter focuses on factors, including culture medium, sample preparation, data acquisition, and data analysis that affect strain-level characterization. In addition, the chapter hi
作者: athlete’s-foot    時間: 2025-3-22 06:04
Discriminatory Power of MALDI-TOF Mass Spectrometry for Phylogenetically Closely Related Microbial Svel requires rigorous standardization, high spectral quality and the application of adequate sample-processing methods, spectra acquisition procedures, and an optimized data analysis pipeline that includes multivariate fingerprinting approaches. However, these conditions cannot be expected in a rout
作者: RAFF    時間: 2025-3-22 10:16

作者: 災(zāi)禍    時間: 2025-3-22 13:53
Bacterial Identification at the Serovar Level by Top-Down Mass Spectrometrytion of un-sequenced or newly acquired SNPs and plasmid proteins that may be specific to a given strain without prior knowledge of the sample. Subsequent identification of expressed proteins, serovar-specific biomarkers, and post-translational modifications by top-down LC-MS/MS is integral to rapid
作者: Retrieval    時間: 2025-3-22 20:23

作者: 變色龍    時間: 2025-3-22 23:31
Stable-Isotope-Based Strategies for Rapid Determination of Drug Resistance by Mass Spectrometrybiomarker masses for phages, initially proliferated in isotopically manipulated growth medium and subsequently grown in microorganisms in growth medium with elements of natural abundance ratios, is monitored and is an indication of drug resistance. Advantages of these assays as well as tools for aut
作者: 玩笑    時間: 2025-3-23 04:22

作者: Debark    時間: 2025-3-23 05:38
Bernhard Kornelius M.A.,Dieter Rothd biomolecules in microbial mixtures or complex biological samples. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is the method of choice for the rapid identification of microorganisms. This chapter concludes with a brief discussion of the selection of MALDI matrices and m
作者: 職業(yè)    時間: 2025-3-23 11:21
Reimut Zohlnh?fer,Christoph Egle M.A.ng such peptides to reference microbial proteomes. Phylogenomic profiles of sequenced peptides may be then analyzed using numerical taxonomy tools to reveal strain identities up to subspecies level. The results thereof reveal not only the taxonomic position of an investigated strain but also allow f
作者: 2否定    時間: 2025-3-23 17:37

作者: 共同確定為確    時間: 2025-3-23 21:08
,Looking Ahead: 2022–2035 and Beyond,vel requires rigorous standardization, high spectral quality and the application of adequate sample-processing methods, spectra acquisition procedures, and an optimized data analysis pipeline that includes multivariate fingerprinting approaches. However, these conditions cannot be expected in a rout
作者: Graphite    時間: 2025-3-23 22:30

作者: 嫻熟    時間: 2025-3-24 05:59
Claire Hooker,Chris Degeling,Paul Masontion of un-sequenced or newly acquired SNPs and plasmid proteins that may be specific to a given strain without prior knowledge of the sample. Subsequent identification of expressed proteins, serovar-specific biomarkers, and post-translational modifications by top-down LC-MS/MS is integral to rapid
作者: crutch    時間: 2025-3-24 10:22
Claire Hooker,Chris Degeling,Paul Masonlarity. Two main approaches have been applied to resolve mass peak variations below the species level: the library-based and the proteomics-based approach. Within the library-based approach, both sample pre-treatment and data reduction strategies have been developed. Proteomics-based approaches comp
作者: anthesis    時間: 2025-3-24 12:00
Claire Hooker,Chris Degeling,Paul Masonbiomarker masses for phages, initially proliferated in isotopically manipulated growth medium and subsequently grown in microorganisms in growth medium with elements of natural abundance ratios, is monitored and is an indication of drug resistance. Advantages of these assays as well as tools for aut
作者: cognizant    時間: 2025-3-24 15:33

作者: DUCE    時間: 2025-3-24 20:40

作者: Accede    時間: 2025-3-25 01:47

作者: 民間傳說    時間: 2025-3-25 05:50
Sample Preparation Methods for the Rapid MS Analysis of Microorganismsent sample preparation steps used in the context of rapid analysis of microorganisms by mass spectrometry (MS). Sample preparation methods that might eliminate the need for culturing of the target cells (the key rate-limiting step) to obtain detectable signals are important for the rapid analysis of
作者: 1FAWN    時間: 2025-3-25 08:54
Advantages Offered by Proteomic Strategies for Rapid Biodetectioneliable than library matching. Identifications can be made without controlling sample preparation or instrumental variables. One rapidly growing database supports all ionization techniques. In addition, specific biomarkers can be identified for strain identification and forensics. The advantages of
作者: incubus    時間: 2025-3-25 11:45
Bottom-Up Proteomics Methods for Strain-Level Typing and Identification of Bacteria-based methods provide an attractive alternative to well-established microbiological procedures and are characterized by the relatively high speed of acquiring taxonomically relevant information. Bottom-up, shotgun-proteomics techniques allow for high-throughput sequencing of peptides from protease-
作者: delusion    時間: 2025-3-25 17:16

作者: Fsh238    時間: 2025-3-25 22:07

作者: 舊式步槍    時間: 2025-3-26 00:12

作者: Incommensurate    時間: 2025-3-26 07:04
MALDI-TOF MS as a Novel Tool for Dereplication and Characterization of Microbiota in Bacterial Diverionally, this involves first a dereplication step in which all isolates are examined to group those that represent the same taxon in a rapid and cost-effective manner. Dereplication therefore is the assessment of novelty and aims to reduce a large number of isolates to a smaller, nonredundant set fo
作者: neolith    時間: 2025-3-26 08:49

作者: inferno    時間: 2025-3-26 13:54

作者: Obituary    時間: 2025-3-26 17:41
Detection of β-Lactamases and Their Activity Using MALDI-TOF MS, their fast and reliable detection is important for prevention of their uncontrolled spread. Here, we describe two methods based on matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry allowing functional detection of hydrolysis products of β-lactams as well as a
作者: MAG    時間: 2025-3-26 23:17

作者: Genteel    時間: 2025-3-27 01:51

作者: buoyant    時間: 2025-3-27 06:53

作者: Intrepid    時間: 2025-3-27 12:59

作者: 支柱    時間: 2025-3-27 15:14
Advantages Offered by Proteomic Strategies for Rapid Biodetectionase supports all ionization techniques. In addition, specific biomarkers can be identified for strain identification and forensics. The advantages of proteomic strategies are illustrated in the analysis of components in mixtures, genetic engineering in bacteria, and bacteria with unsequenced genomes.
作者: 誓言    時間: 2025-3-27 17:53

作者: 天氣    時間: 2025-3-28 00:28
Detection of β-Lactamases and Their Activity Using MALDI-TOF MS important for treatment and setting up barrier precautions preventing their spread is possible. On the contrary, direct enzyme molecular signal monitoring is reliable for epidemiological studies, typing of the isolates, and precise identification of β-lactamase type.
作者: single    時間: 2025-3-28 02:37
Methods and Instrumentation in Mass Spectrometry for the Differentiation of Closely Related Microorgted bacteria at the strain level and antibiotic-resistant/susceptible bacteria. An overview of the MS instrumentation will be presented in the context of microorganism detection and identification with selected examples from the current scientific literature.
作者: Cardioplegia    時間: 2025-3-28 08:03

作者: 使殘廢    時間: 2025-3-28 13:03
https://doi.org/10.1007/978-3-319-52417-7erize microorganisms at the species level or above, this book focuses on strain-level and subtyping applications. In this introduction, a short summary of each individual chapter is presented. MS methods and approaches with potential to impact future clinical applications, not covered explicitly in the book, are also briefly reviewed.
作者: FILTH    時間: 2025-3-28 16:34

作者: correspondent    時間: 2025-3-28 18:46
Introduction,erize microorganisms at the species level or above, this book focuses on strain-level and subtyping applications. In this introduction, a short summary of each individual chapter is presented. MS methods and approaches with potential to impact future clinical applications, not covered explicitly in the book, are also briefly reviewed.
作者: 紅潤    時間: 2025-3-29 00:54
Modulation of the Discriminatory Power of MALDI-TOF MS Profiling for Distinguishing Between Closely otypes led to the development of two alternative sample preparation protocols. By switching to an alternative matrix solution or by microwave-assisted tryptic digestion of the cellular material, these simple approaches enabled detection of new specific signals.
作者: 殺蟲劑    時間: 2025-3-29 04:38
https://doi.org/10.1007/978-3-319-26070-9Mass Spectrometry; Strain-level focus; Microorganism Characterization; MS-based Assay; infectious diseas
作者: 哄騙    時間: 2025-3-29 10:00

作者: anesthesia    時間: 2025-3-29 13:03

作者: 神化怪物    時間: 2025-3-29 19:23
Children in Violent Circumstances,ex problems in microbiology. The successful application of MS for the analysis of microorganisms involves a concerted selection of hardware along with the development of methodology. This chapter will focus on this interplay between MS instrumentation and methodology for the analysis of closely rela
作者: 不愿    時間: 2025-3-29 20:50

作者: Comedienne    時間: 2025-3-30 02:47

作者: 詼諧    時間: 2025-3-30 07:58

作者: acrimony    時間: 2025-3-30 08:39
Unwiederholbarkeit, das letzte Paradies,l for characterizing diverse bacteria. Historically, the primary application of this technology has been to identify bacteria at the genus and species levels in a few minutes. The most commonly used identification method involves matching mass spectra of unknown bacteria to reference spectra in comm




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