標(biāo)題: Titlebook: Antigen Processing; Methods and Protocol Peter van Endert Book 2019Latest edition Springer Science+Business Media, LLC, part of Springer Na [打印本頁(yè)] 作者: obesity 時(shí)間: 2025-3-21 17:34
書(shū)目名稱Antigen Processing影響因子(影響力)
作者: Saline 時(shí)間: 2025-3-21 22:24
https://doi.org/10.1007/978-3-319-40832-3 in the analysis of MHC class I molecule biosynthesis and degradation as well as complementary studies using conventional conformationally specific antibodies. Coupled together with pharmacological manipulation which can target both biosynthetic and degradative pathways, this offers a powerful tool in analyzing MHC class I molecules.作者: 好色 時(shí)間: 2025-3-22 02:26 作者: 縮短 時(shí)間: 2025-3-22 07:11
Trimming of MHC Class I Ligands by ERAP Aminopeptidases,function of ERAPs in peptide repertoire development. Specifically, our assay allows for monitoring trimming of free but also of MHC I-bound peptides which may reflect the physiological situation best.作者: JIBE 時(shí)間: 2025-3-22 12:29 作者: 鈍劍 時(shí)間: 2025-3-22 14:28
Identification of MHC Ligands and Establishing MHC Class I Peptide Motifs,ple, the ligands of monoallelic C1R cells and multiallelic peripheral blood mononuclear cell tissue will be identified and a scoring matrix for the prediction of HLA-C*01:02-presented T cell epitopes will be developed.作者: AUGER 時(shí)間: 2025-3-22 18:48 作者: aerial 時(shí)間: 2025-3-23 01:07 作者: fulcrum 時(shí)間: 2025-3-23 04:24
https://doi.org/10.1007/978-3-319-40832-3tionated using reverse phase high performance liquid chromatography and detected by the activation of CD8+ T cell hybridomas. The results provide information on the structure and amount of the peptides and yield insights into the mechanisms that generate the naturally processed peptides.作者: absorbed 時(shí)間: 2025-3-23 06:00 作者: Panacea 時(shí)間: 2025-3-23 12:13
Book 2019Latest editionnic peptides produced in the standard processing pathways for MHC class I and II molecules. The new chapters cover topics such as biochemical and cellular approaches to study the impact of the endoplasmic reticulum aminopeptidases; techniques to monitor MHC class I synthesis and degradation; approac作者: Functional 時(shí)間: 2025-3-23 16:48 作者: 致敬 時(shí)間: 2025-3-23 19:10 作者: 責(zé)難 時(shí)間: 2025-3-24 00:19
https://doi.org/10.1007/978-3-662-33043-2eral diseases including autoimmune diseases, viral infections, and virally induced cancers. In this chapter, we describe two basic methods for monitoring peptide-trimming activity by ER aminopeptidases and screening potential chemical inhibitors.作者: 不可接觸 時(shí)間: 2025-3-24 03:01
Chapter 3 The Togetherness of Trustce proteins can be used to precisely probe various aspects of antigen presentation, including the kinetics of peptide generation, MHC class I surface stability, and presentation efficiency following pharmacological and genetic manipulations including genome wide and high throughput drug screening.作者: Introvert 時(shí)間: 2025-3-24 07:51
Shiu Yeh Yu,C. N. Sun,Murtie F. Stillreview the existing strategies for the isolation of MHC-restricted peptides and provide a detailed protocol for the immunoaffinity purification of MHC class I- and II-presented peptides from primary tissues or cells.作者: STELL 時(shí)間: 2025-3-24 14:19 作者: 刀鋒 時(shí)間: 2025-3-24 15:07 作者: 捕鯨魚(yú)叉 時(shí)間: 2025-3-24 19:22
Purification of Large Cytosolic Proteases for In Vitro Assays: 20S and 26S Proteasomes,g. Here, we describe the purification of active human 20S and 26S proteasomes from human erythrocytes by DEAE-ion exchange chromatography, ammonium sulfate precipitation, glycerol density gradient centrifugation, and Superose-6 size exclusion chromatography and their characterization using fluorogenic substrates and specific inhibitors.作者: 在駕駛 時(shí)間: 2025-3-24 23:56
Peptide Trimming for MHC Class I Presentation by Endoplasmic Reticulum Aminopeptidases,eral diseases including autoimmune diseases, viral infections, and virally induced cancers. In this chapter, we describe two basic methods for monitoring peptide-trimming activity by ER aminopeptidases and screening potential chemical inhibitors.作者: GIDDY 時(shí)間: 2025-3-25 04:22 作者: 肉身 時(shí)間: 2025-3-25 08:34 作者: MIME 時(shí)間: 2025-3-25 12:53
Evaluating CD8+ T Cell Responses In Vitro,ology and killing pathways has more recently resulted in the design of reliable nonradioactive tests to analyze CD8. T cell responses. Allowing for real-time evaluation of target cell viability, some of them are particularly attractive and are described here together with traditional assays.作者: 去才蔑視 時(shí)間: 2025-3-25 19:34 作者: Recessive 時(shí)間: 2025-3-25 21:08 作者: 分期付款 時(shí)間: 2025-3-26 03:10 作者: 側(cè)面左右 時(shí)間: 2025-3-26 06:54 作者: 培養(yǎng) 時(shí)間: 2025-3-26 09:02
Monitoring MHC-II Endocytosis and Recycling Using Cell-Surface Protein Biotinylation-Based Assays,nt that contains a cleavable disulfide bond that allows for reversible labeling of proteins. Biotinylation is rapid and stable, and does not transfer from cell to cell, and the small size of the biotin probe does not affect cell function.作者: acheon 時(shí)間: 2025-3-26 15:10 作者: BRIBE 時(shí)間: 2025-3-26 20:19 作者: figure 時(shí)間: 2025-3-27 00:06
In Vitro Studies of MHC Class I Peptide Loading and Exchange,molecular level how antigenic peptides are selected for presentation to CD8+ T-cells. Here, we describe practical procedures for generating a cell-free system made up of MHC class I molecules and tapasin that can be used for mechanistic studies of peptide loading and exchange.作者: 立即 時(shí)間: 2025-3-27 04:15 作者: Cacophonous 時(shí)間: 2025-3-27 07:15 作者: 懶洋洋 時(shí)間: 2025-3-27 09:26 作者: Germinate 時(shí)間: 2025-3-27 17:37
F. Lamy,D. Gibson,M. Ledoux,J. C. Moreuxely, the numerous advantages of poxvirus recombinants have made the Vaccinia expression system a mainstay in the study of processing and presentation over the past two decades. In an attempt to address one shortcoming of Vaccinia virus while simultaneously retaining the benefits inherent to poxvirus作者: 重力 時(shí)間: 2025-3-27 18:29
1064-3745 ing known pitfalls..?.Cutting-edge and comprehensive, .Antigen Processing: Methods and Protocols, Second Edition. is a valuable tool for both novice and expert researchers interested in studying antigen processing and venturing out further into this evolving field..978-1-4939-9452-6978-1-4939-9450-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 暴行 時(shí)間: 2025-3-27 23:09 作者: hypnogram 時(shí)間: 2025-3-28 02:12
Quantitating MHC Class I Ligand Production and Presentation Using TCR-Like Antibodies,icity for peptide–MHC complexes are created, this method will be helpful in quantifying the exact numbers of complexes generated by cell types and relating these numbers to physiological outcomes of T cell activation.作者: irritation 時(shí)間: 2025-3-28 09:42
A Flow Cytometry-Based Approach to Unravel Viral Interference with the MHC Class I Antigen Processir MHC I downregulation has been identified, the same cells can be used to elucidate the mechanism of action. The stage at which interference with antigen processing occurs can be identified using specific assays. An essential step frequently targeted by viruses is the translocation of peptides into 作者: 美色花錢 時(shí)間: 2025-3-28 10:42 作者: oblique 時(shí)間: 2025-3-28 14:34 作者: entail 時(shí)間: 2025-3-28 22:37 作者: Adjourn 時(shí)間: 2025-3-29 01:38 作者: –DOX 時(shí)間: 2025-3-29 04:27 作者: cumulative 時(shí)間: 2025-3-29 10:04
Peptide Trimming for MHC Class I Presentation by Endoplasmic Reticulum Aminopeptidases, trimming peptide ligands for MHC class I molecules. Functional polymorphisms in ERAP1 and ERAP2 genes have been associated with predisposition to several diseases including autoimmune diseases, viral infections, and virally induced cancers. In this chapter, we describe two basic methods for monitor作者: 全部逛商店 時(shí)間: 2025-3-29 13:03 作者: 大門在匯總 時(shí)間: 2025-3-29 19:31 作者: 大包裹 時(shí)間: 2025-3-29 23:21 作者: 處理 時(shí)間: 2025-3-30 00:36 作者: 未成熟 時(shí)間: 2025-3-30 07:58 作者: BLUSH 時(shí)間: 2025-3-30 08:33 作者: 揭穿真相 時(shí)間: 2025-3-30 15:18
Identification of MHC Ligands and Establishing MHC Class I Peptide Motifs,l an invaluable tool for the identification of disease-associated epitopes ranging from pathogen associated epitopes, tumor associated natural and neoepitopes to autoimmune disease associated epitopes. As a matter of fact, the vast majority of T cell epitopes discovered during the past two decades w作者: irritation 時(shí)間: 2025-3-30 18:56
Quantitating MHC Class I Ligand Production and Presentation Using TCR-Like Antibodies,als that alter the antigen presentation machinery. Here I describe a quantitative flow cytometry application for determining the number of peptide–MHC complexes on the surface of cells grown in tissue culture that express an endogenous protein from which the peptide is derived. The procedure require作者: GNAT 時(shí)間: 2025-3-30 23:57
Identifying Antigens Recognized by Cytolytic T Lymphocytes on Tumors,y approaches. This chapter provides a global approach for the identification of peptides recognized by CTL. It implies the identification of the HLA molecule presenting the peptide as well as the design and screening of a cDNA library derived from the tumor cells. Methods used for the identification作者: Anhydrous 時(shí)間: 2025-3-31 01:15
A Flow Cytometry-Based Approach to Unravel Viral Interference with the MHC Class I Antigen Processig viral infection, MHC class I molecules carry and display viral peptides at the cell surface. CD8. T cells that recognize these peptides will eliminate the virus-infected cells. Viruses counteract this highly sophisticated host detection system by downregulating cell surface expression of MHC class作者: HAIRY 時(shí)間: 2025-3-31 05:28
Evaluating CD8+ T Cell Responses In Vitro, laboratories. Whereas other radioactive tests were later on described, they never ousted the .Cr-release assay. More thorough understanding of CTL biology and killing pathways has more recently resulted in the design of reliable nonradioactive tests to analyze CD8. T cell responses. Allowing for re作者: absolve 時(shí)間: 2025-3-31 12:34 作者: DOSE 時(shí)間: 2025-3-31 14:42 作者: 獨(dú)輪車 時(shí)間: 2025-3-31 20:39
