標(biāo)題: Titlebook: Animal Endo-SiRNAs; Methods and Protocol Andreas Werner Book 2014 Springer Science+Business Media New York 2014 C. elegans.Dicer molecule.S [打印本頁] 作者: Constrict 時間: 2025-3-21 18:48
書目名稱Animal Endo-SiRNAs影響因子(影響力)
書目名稱Animal Endo-SiRNAs影響因子(影響力)學(xué)科排名
書目名稱Animal Endo-SiRNAs網(wǎng)絡(luò)公開度
書目名稱Animal Endo-SiRNAs網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Animal Endo-SiRNAs被引頻次
書目名稱Animal Endo-SiRNAs被引頻次學(xué)科排名
書目名稱Animal Endo-SiRNAs年度引用
書目名稱Animal Endo-SiRNAs年度引用學(xué)科排名
書目名稱Animal Endo-SiRNAs讀者反饋
書目名稱Animal Endo-SiRNAs讀者反饋學(xué)科排名
作者: 提升 時間: 2025-3-21 21:43 作者: MEEK 時間: 2025-3-22 00:23 作者: Atheroma 時間: 2025-3-22 07:38 作者: 舊式步槍 時間: 2025-3-22 12:42 作者: Jubilation 時間: 2025-3-22 15:53
Craig H. Faunce,Craig A. Laymanethod yields pure fractions of CBs, and it is robust, reproducible and does not require special equipment or abundant starting material. The CB is packed with large amounts of RNA, especially small RNAs. Isolation of the CBs provides a tool to enrich these RNA species.作者: 意外 時間: 2025-3-22 18:07
Ecological Crisis Through a Social Lens,tly detecting endo-siRNA levels in bulk, as well as an optimized qPCR method for detecting the effect of endo-siRNAs on gene targets. Intriguingly, the loss of endo-siRNAs frequently results in enhanced experimental RNAi. Thus, we also present an optimized method to assess the indirect impact of endo-siRNAs on experimental RNAi efficiency.作者: Limited 時間: 2025-3-23 00:15
The empirical value of diversity measures,orthern blotting using DIG-labelled RNA probes. Moreover, it includes a strategy to design and generate cheap hybridization probes with greatly increased sensitivity. These methods may be used as a simple and robust protocol for nonradioactive detection of small RNAs or be combined with other strategies to potentially enhance signal intensity.作者: 頌揚(yáng)國家 時間: 2025-3-23 02:30
The Economic System and the Environmentalso provide guidance for analysis of primary bisulfite sequencing data and interpretation of the methylation status using the Web-based bisulfite sequencing DNA methylation (BISMA) analysis. This refined and reproducible assay can be performed even using a small amount of genomic DNA and is suitable for the analysis of clinical tissue samples.作者: 招人嫉妒 時間: 2025-3-23 07:57 作者: emission 時間: 2025-3-23 10:59 作者: APO 時間: 2025-3-23 17:57 作者: Abutment 時間: 2025-3-23 21:46 作者: 阻塞 時間: 2025-3-24 01:07
Hugo Rosa Da Concei??o,Jan B?rnerligonucleotide probes consisting of combinations of LNA and 2′-O-methyl RNAs that under optimized hybridization buffer conditions can provide a highly sensitive assay performance with only 1 h hybridization time.作者: 迅速成長 時間: 2025-3-24 05:09 作者: 反感 時間: 2025-3-24 10:01 作者: gangrene 時間: 2025-3-24 13:18
Enhanced Detection of Small RNAs Using a Nonradioactive Approach,cross-linking method (EDC) and digoxigenin (DIG) labeling, and it can detect small RNAs with concentrations as low as 0.05 fmol and requires as little as a few seconds of membrane exposure for signal generation.作者: 我悲傷 時間: 2025-3-24 16:14 作者: intertwine 時間: 2025-3-24 22:26 作者: Flat-Feet 時間: 2025-3-25 03:10
https://doi.org/10.1007/978-3-030-35379-7 sequencing libraries. We also present a biochemical purification protocol for enriching 5′ tRFs and separating them from miRNAs. And finally, we suggest reliable methods for detecting and quantifying tRFs.作者: Freeze 時間: 2025-3-25 04:29 作者: nepotism 時間: 2025-3-25 07:41
Thorsten Dittmar,Boris Koch,Rudolf Jaffétheir similarity with miRNAs and products of physiological RNA turn-over, endo-siRNAs are difficult to investigate. Here, we report a system, oocytes from ., that allows for the generation and analysis of endo-siRNAs from double-stranded RNA precursors.作者: 蘆筍 時間: 2025-3-25 12:42
Ecological Crisis Through a Social Lens,ovides the identity and quantity of a particular class of small RNAs. In this chapter we describe a detailed protocol for preparing small RNA libraries for deep sequencing on the Illumina platform from the nematode ..作者: adroit 時間: 2025-3-25 19:39
Generation of Endo-siRNAs in , Oocytes,their similarity with miRNAs and products of physiological RNA turn-over, endo-siRNAs are difficult to investigate. Here, we report a system, oocytes from ., that allows for the generation and analysis of endo-siRNAs from double-stranded RNA precursors.作者: 抵押貸款 時間: 2025-3-25 23:39 作者: 白楊魚 時間: 2025-3-26 02:37 作者: atopic-rhinitis 時間: 2025-3-26 05:10 作者: legitimate 時間: 2025-3-26 10:23
Generation of Endo-siRNAs in , Oocytes, is much less clear. They are thought to be produced by Dicer and to contribute to transposon silencing. Because of their generally low abundance and their similarity with miRNAs and products of physiological RNA turn-over, endo-siRNAs are difficult to investigate. Here, we report a system, oocytes 作者: 省略 時間: 2025-3-26 13:08 作者: antiquated 時間: 2025-3-26 17:39 作者: 假設(shè) 時間: 2025-3-26 22:53 作者: Esophagus 時間: 2025-3-27 02:06
Assays for Direct and Indirect Effects of , Endo-siRNAs,) have emerged in the last few years as a largely independent class of small RNAs that regulate endogenous gene expression, with mechanisms distinct from those of piRNAs and miRNAs. Quantification of these small RNAs and their effect on target RNAs is a powerful tool for the analysis of RNAi; howeve作者: 失眠癥 時間: 2025-3-27 07:40 作者: nauseate 時間: 2025-3-27 11:48 作者: 固執(zhí)點好 時間: 2025-3-27 14:06 作者: transdermal 時間: 2025-3-27 19:48 作者: dagger 時間: 2025-3-28 00:53
Computing siRNA and piRNA Overlap Signatures,nate. A bioinformatic approach revealed a strong tendency of sense and antisense piRNAs to overlap with each other over ten nucleotides and had a major role in understanding the mechanisms of piRNA biogenesis. Using similar approaches, it is possible to detect a tendency of sense and antisense siRNA作者: Eulogy 時間: 2025-3-28 03:41 作者: kindred 時間: 2025-3-28 08:40
Computational Analysis, Biochemical Purification, and Detection of tRNA-Derived Small RNA Fragmentscs tools for their detection. Small RNAs generated from tRNAs, transfer RNA-derived fragments (tRFs), represent a novel challenge in accurately identifying and distinguishing them from random degradation products of tRNAs. Here, we describe a bioinformatics approach to detect tRFs in next-generation作者: 分開如此和諧 時間: 2025-3-28 13:04 作者: Astigmatism 時間: 2025-3-28 17:14
Animal Endo-SiRNAs978-1-4939-0931-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: ADORN 時間: 2025-3-28 20:09
https://doi.org/10.1007/978-1-4939-0931-5C; elegans; Dicer molecule; Short interfering RNAs (siRNAs); endo-siRNAs; gene expression; model systems; 作者: 取回 時間: 2025-3-28 22:55
978-1-4939-4160-5Springer Science+Business Media New York 2014作者: 他一致 時間: 2025-3-29 03:33
https://doi.org/10.1007/978-90-481-2406-0tic modifications to particular loci in the genome. Classical examples of such regulation are X-chromosome inactivation and genomic imprinting; however it is now clear that ncRNAs exert their influence over a wider array of genes throughout the metazoan genome. Accumulating evidence suggests that th作者: 代理人 時間: 2025-3-29 11:02 作者: Digitalis 時間: 2025-3-29 11:51 作者: Dysplasia 時間: 2025-3-29 16:07 作者: endocardium 時間: 2025-3-29 21:18 作者: GLOSS 時間: 2025-3-30 01:04
Ecological Crisis Through a Social Lens, wide array of cellular functions. Because of the abundance of these small RNAs, library preparation from an RNA sample followed by deep sequencing provides the identity and quantity of a particular class of small RNAs. In this chapter we describe a detailed protocol for preparing small RNA librarie作者: macular-edema 時間: 2025-3-30 07:43 作者: OMIT 時間: 2025-3-30 10:26 作者: 左右連貫 時間: 2025-3-30 16:06
The empirical value of diversity measures,ng suppressor that binds small interfering RNA (siRNA) with high affinity. A bifunctional p19 fusion protein, with an N-terminal maltose binding protein (MBP) and a C-terminal chitin binding domain (CBD) allows protein purification and binding of p19 to chitin magnetic beads via the chitin binding d作者: Nuance 時間: 2025-3-30 17:49
Hugo Rosa Da Concei??o,Jan B?rnern-fixed paraffin-embedded (FFPE) samples of tissue. Particularly the recently discovered classes of noncoding RNA (ncRNA) including endo-siRNAs and microRNAs require such a technique to enable their study and visualization in natural contexts, and in the last decade, many advances have been made, in作者: 無所不知 時間: 2025-3-30 21:04 作者: antipsychotic 時間: 2025-3-31 01:00 作者: minaret 時間: 2025-3-31 06:20 作者: Condescending 時間: 2025-3-31 10:12 作者: 正式通知 時間: 2025-3-31 17:07
The Economic System and the Environment simple genome-wide DNA methylation assay that allows for a precise quantitative analysis of differences in the promoter of human long interspersed nuclear element 1 (LINE-1 or L1) retrotransposons in response to endogenous and exogenous expression of endo-siRNAs. Using the DNA bisulfite modificatio作者: 最有利 時間: 2025-3-31 17:37
Andreas WernerIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: BABY 時間: 2025-4-1 00:19
