標(biāo)題: Titlebook: Alpha-Synuclein; Methods and Protocol Tim Bartels Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 endocytosis. [打印本頁(yè)] 作者: Blandishment 時(shí)間: 2025-3-21 18:24
書目名稱Alpha-Synuclein影響因子(影響力)
書目名稱Alpha-Synuclein影響因子(影響力)學(xué)科排名
書目名稱Alpha-Synuclein網(wǎng)絡(luò)公開度
書目名稱Alpha-Synuclein網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Alpha-Synuclein被引頻次
書目名稱Alpha-Synuclein被引頻次學(xué)科排名
書目名稱Alpha-Synuclein年度引用
書目名稱Alpha-Synuclein年度引用學(xué)科排名
書目名稱Alpha-Synuclein讀者反饋
書目名稱Alpha-Synuclein讀者反饋學(xué)科排名
作者: Brain-Waves 時(shí)間: 2025-3-21 23:12 作者: Interdict 時(shí)間: 2025-3-22 03:13
1064-3745 , .Alpha-Synuclein: Methods and Protocols. is a valuable reference guide that aids researchers working on topics related to alpha-synuclein and its various aspects..978-1-4939-9124-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 原諒 時(shí)間: 2025-3-22 06:39
Book 2019ily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and thorough, .Alpha-Synuclein: Methods and Protocols. is a valuable reference guide that aids researchers working on topics related to alpha-synuclein and its various aspects..作者: LAY 時(shí)間: 2025-3-22 12:40 作者: 大笑 時(shí)間: 2025-3-22 13:10
Economic Material Use in Injection Moulding,on procedures for initiating aSyn inclusion formation at various regions of the mouse brain using computer-assisted motorized stereotaxic microinjection of aSyn PFFs and discuss appropriate strategies for controls and analysis.作者: FAZE 時(shí)間: 2025-3-22 17:02 作者: 相一致 時(shí)間: 2025-3-23 01:14 作者: 共同給與 時(shí)間: 2025-3-23 01:54 作者: Creatinine-Test 時(shí)間: 2025-3-23 09:26
Developments in Injection Moulding—1gates from human CSF samples. Given the amplificative nature of the technique, minute amounts of misfolded protein aggregates circulating in human bodily fluids can be multiplied and thereafter detected by more conventional methods, such as immune assays or fluorescence. Following these principles, 作者: 希望 時(shí)間: 2025-3-23 10:01
Economic Material Use in Injection Moulding,ound in several neurodegenerative disorders including Parkinson’s disease (PD). Emerging evidence indicates that aSyn amyloid fibrils, a configuration that is present within these characteristic inclusions, are capable of self-replicating by templating the conversion of endogenously expressed aSyn i作者: hypnogram 時(shí)間: 2025-3-23 14:59 作者: DAUNT 時(shí)間: 2025-3-23 20:45
Developments in Injection Moulding—3 . in diseased brain has been a challenge in the field for some time. Here we describe a method, the alpha-synuclein proximity ligation assay (AS-PLA), to detect alpha-synuclein oligomers in paraffin-embedded brain sections. Using AS-PLA previously unobserved alpha-synuclein oligomeric pathology is 作者: 克制 時(shí)間: 2025-3-24 00:36 作者: 癡呆 時(shí)間: 2025-3-24 05:07 作者: MULTI 時(shí)間: 2025-3-24 07:34 作者: 享樂(lè)主義者 時(shí)間: 2025-3-24 14:32 作者: 人類 時(shí)間: 2025-3-24 16:13 作者: PHONE 時(shí)間: 2025-3-24 21:51
Mauricio Diaz Arancibia,Pinar Okumus states, with hitherto unclear physiological role of such conformational changes. Due to its ability to provide rich structural information for proteins in their near-native environment, nuclear magnetic resonance (NMR) spectroscopy has been a valuable tool to study these conformational states. In t作者: 準(zhǔn)則 時(shí)間: 2025-3-24 23:59 作者: 描繪 時(shí)間: 2025-3-25 06:39
https://doi.org/10.1007/978-3-030-59169-4in dopaminergic and other types of neurons. Because α-synuclein accumulation leads to a toxic gain of function, its ectopic expression in . has been a useful in vivo model for testing modifiers of its toxicity. This chapter describes four assays: the rapid iterative negative geotaxis, rough eye phen作者: 沖突 時(shí)間: 2025-3-25 11:09 作者: neurologist 時(shí)間: 2025-3-25 13:42
R. Hamid,Y. Khairullah,A. R. Khalimin the immediate vicinity of αSyn in living rat cortical neurons. To isolate these interactions, we transduced primary cortical neurons with a lentivirus encoding APEX2 tagged to the C-terminus of alpha-synuclein (αSyn) and under the control of a synapsin promoter. Neural protein lysates were then i作者: 捏造 時(shí)間: 2025-3-25 18:23
A. I. Yilmaz,U. Albostan,A. Caner,O. Kurc interactions of intrinsically disordered proteins as alpha-synuclein..Here we describe all steps required for a corresponding study: the spin labeling procedure, sample preparation, spectroscopic experimental procedure, and data analysis.作者: 擦試不掉 時(shí)間: 2025-3-25 21:07 作者: 我不重要 時(shí)間: 2025-3-26 03:50
Methods in Molecular Biologyhttp://image.papertrans.cn/a/image/153908.jpg作者: 壯觀的游行 時(shí)間: 2025-3-26 05:22 作者: 我正派 時(shí)間: 2025-3-26 08:33
Developments in Injection Moulding—3 . in diseased brain has been a challenge in the field for some time. Here we describe a method, the alpha-synuclein proximity ligation assay (AS-PLA), to detect alpha-synuclein oligomers in paraffin-embedded brain sections. Using AS-PLA previously unobserved alpha-synuclein oligomeric pathology is revealed.作者: 油氈 時(shí)間: 2025-3-26 15:53 作者: Inculcate 時(shí)間: 2025-3-26 19:38
A. I. Yilmaz,U. Albostan,A. Caner,O. Kurc interactions of intrinsically disordered proteins as alpha-synuclein..Here we describe all steps required for a corresponding study: the spin labeling procedure, sample preparation, spectroscopic experimental procedure, and data analysis.作者: 不要不誠(chéng)實(shí) 時(shí)間: 2025-3-26 21:51 作者: 無(wú)聊的人 時(shí)間: 2025-3-27 03:30
Recombinant Expression and Purification of ,-Acetylated Alpha-Synuclein,n systems such as . would not normally be Nt-acetylated as these cells lack the appropriate .-α-terminal acetylation complex. Here we describe a simple protocol that allows the recombinant expression and purification of Nt-acetylated alpha-synuclein (aS) from ..作者: 詢問(wèn) 時(shí)間: 2025-3-27 06:12
Probing Alpha-Synuclein Conformations by Electron Paramagnetic Resonance (EPR) Spectroscopy, interactions of intrinsically disordered proteins as alpha-synuclein..Here we describe all steps required for a corresponding study: the spin labeling procedure, sample preparation, spectroscopic experimental procedure, and data analysis.作者: 減至最低 時(shí)間: 2025-3-27 12:14 作者: 尾隨 時(shí)間: 2025-3-27 16:29 作者: 滑動(dòng) 時(shí)間: 2025-3-27 18:59
https://doi.org/10.1007/978-3-030-59169-4Here we describe a method using mass spectrometry to characterize and quantify immuno-enriched α-synuclein forms from biochemically fractionated brain tissue.作者: Working-Memory 時(shí)間: 2025-3-28 01:31 作者: outset 時(shí)間: 2025-3-28 06:01 作者: Foreknowledge 時(shí)間: 2025-3-28 07:34 作者: ALERT 時(shí)間: 2025-3-28 11:28
Kinetic Measurements of Endocytosis and Exocytosis in Cultured Neurons,ws us to study exocytosis and endocytosis in neurons. When neurons are transfected with this protein we can measure the rate of vesicles fusing and internalizing from the membrane using live total internal reflection fluorescence (TIRF) imaging. Here, we describe transfection, culturing, and imaging作者: exercise 時(shí)間: 2025-3-28 16:14 作者: bonnet 時(shí)間: 2025-3-28 19:40
Stereotaxic Targeting of Alpha-Synuclein Pathology in Mouse Brain Using Preformed Fibrils,ound in several neurodegenerative disorders including Parkinson’s disease (PD). Emerging evidence indicates that aSyn amyloid fibrils, a configuration that is present within these characteristic inclusions, are capable of self-replicating by templating the conversion of endogenously expressed aSyn i作者: 殘暴 時(shí)間: 2025-3-29 00:32
Quantification of Alpha-Synuclein in Biological Fluids by Electrochemiluminescence-Based Detection, constituent of the intracellular aggregates Lewy bodies, a neuropathological hallmark of PD, and others. The extracellular presence of α-synuclein in blood, CSF, saliva, and conditioned media motivated the hypothesis that the quantification of CSF α-synuclein is a biomarker for α-synuclein-related 作者: Generalize 時(shí)間: 2025-3-29 03:11
Alpha-Synuclein Proximity Ligation Assay (AS-PLA) in Brain Sections to Probe for Alpha-Synuclein Ol . in diseased brain has been a challenge in the field for some time. Here we describe a method, the alpha-synuclein proximity ligation assay (AS-PLA), to detect alpha-synuclein oligomers in paraffin-embedded brain sections. Using AS-PLA previously unobserved alpha-synuclein oligomeric pathology is 作者: receptors 時(shí)間: 2025-3-29 09:37 作者: vascular 時(shí)間: 2025-3-29 15:27 作者: 孤獨(dú)無(wú)助 時(shí)間: 2025-3-29 15:54 作者: 憤慨點(diǎn)吧 時(shí)間: 2025-3-29 20:28
,Circular Dichroism and Isothermal Titration Calorimetry to Study the Interaction of α-Synuclein witnals. It is therefore critical for researchers investigating the determinants of these interactions to rely on methods capable of providing an accurate and complete physicochemical snapshot of the binding events. Circular dichroism (CD) and isothermal titration calorimetry (ITC) are established tech作者: SLING 時(shí)間: 2025-3-30 01:07
Yeast-Based Screens to Target Alpha-Synuclein Toxicity,on of cellular and molecular processes between the budding yeast and higher eukaryotes has made it a valuable system for the investigation of the molecular mechanisms behind various types of devastating human pathologies. Genetic screens in yeast provided important insight into the toxic mechanisms 作者: 含沙射影 時(shí)間: 2025-3-30 06:31 作者: Cuisine 時(shí)間: 2025-3-30 11:06
,Measuring Mitochondrial Dysfunction Caused by Soluble α-Synuclein Oligomers,tween these two phenomena as oligomeric α-synuclein (αSyn) can interact with mitochondria and impair their function. Standardization of methods to prepare αSyn oligomers and isolate functional mitochondria will facilitate efforts to expand upon early findings. Here we present detailed protocols for 作者: Euphonious 時(shí)間: 2025-3-30 15:13
,Monitoring α-Synuclein Proteotoxicity in , Models,in dopaminergic and other types of neurons. Because α-synuclein accumulation leads to a toxic gain of function, its ectopic expression in . has been a useful in vivo model for testing modifiers of its toxicity. This chapter describes four assays: the rapid iterative negative geotaxis, rough eye phen作者: 蔓藤圖飾 時(shí)間: 2025-3-30 20:34 作者: Indolent 時(shí)間: 2025-3-30 20:47 作者: 不要嚴(yán)酷 時(shí)間: 2025-3-31 03:51
Probing Alpha-Synuclein Conformations by Electron Paramagnetic Resonance (EPR) Spectroscopy, interactions of intrinsically disordered proteins as alpha-synuclein..Here we describe all steps required for a corresponding study: the spin labeling procedure, sample preparation, spectroscopic experimental procedure, and data analysis.作者: 凌辱 時(shí)間: 2025-3-31 06:36 作者: 華而不實(shí) 時(shí)間: 2025-3-31 13:04
Hui Yun Chan,Anantharaman Muralidharanion of small unilamellar vesicles (SUVs), mimicking the curved bilayers α-synuclein normally interacts with, the CD-monitored titration of α-synuclein with SUVs, the ITC (lipid-into-protein) experiment, and the subsequent data analysis using an . independent binding site model.作者: chemical-peel 時(shí)間: 2025-3-31 15:51
as a Model for Synucleinopathies and Other Neurodegenerative Diseases: Tools and Techniques, proteotoxicity, while relatively simple molecular, cellular (fractionation studies), metabolic (respiration studies), and behavioral (thrashing and crawling) readouts can be used to study the effects of disease proteins and modifiers more closely.作者: ORE 時(shí)間: 2025-3-31 21:13
,Circular Dichroism and Isothermal Titration Calorimetry to Study the Interaction of α-Synuclein wition of small unilamellar vesicles (SUVs), mimicking the curved bilayers α-synuclein normally interacts with, the CD-monitored titration of α-synuclein with SUVs, the ITC (lipid-into-protein) experiment, and the subsequent data analysis using an . independent binding site model.作者: 手工藝品 時(shí)間: 2025-3-31 23:27 作者: 常到 時(shí)間: 2025-4-1 04:10
Developments in Injection Moulding—1 of wild-type and αβγ-synuclein knockout hippocampal neurons. This technique can be used to evaluate the effect of different phenotypes and treatments in the physiology of synaptic vesicle recycling in cultured neurons.
